Re: [Histonet] Premade Eosin

[Cooper, Brian via Histonet]

When we're not using our Ventana HE600 (their proprietary products), we use 
Eosin Y 515 from Leica.  Our frozen section room uses Protocol Eosin-Y from 
Fisher. 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Piche, Jessica via Histonet  
Sent: Tuesday, April 2, 2024 9:12 AM
To: Histonet 
Subject: [Histonet] Premade Eosin (EXTERNAL EMAIL)

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Good Afternoon,

I hope everyone is having a great week so far!

Can anyone share what pre-made eosin brands they are using and recommend? We 
use Eosin Y. We make it ourselves and want to look into pre-made.

Thank you in advance. Have a great day.

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
Histology Team Leader
203-573-7167
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Re: [Histonet] Bone samples

[Cooper, Brian via Histonet]

Embed the bones diagonally in your molds if you're able (size depending) as 
this will allow for the greatest amount of paraffin support.  Trim them very 
slowly, and if need be, place the blocks into the same "slow decal" solution 
for additional amount of time sufficient to enable better sectioning.  Start 
checking in half hour or 45 minute intervals; rinse the blocks well in running 
water and attempt to section.  If they're not ready, back into decal solution 
they go.  

I feel that very wet ice helps to facilitate sectioning better than ice that is 
drier and fresh out of the freezer.  Just be sure to blot the face of the block 
with gauze before attempting to cut.  

There's my two cents!

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Chakib Boussahmain via Histonet  
Sent: Wednesday, January 24, 2024 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone samples (EXTERNAL EMAIL)

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Hi guys,

 I hope this messagefinds you well. I am currently working on a study involving 
bone samples thathave been treated with slow decal and embedded in paraffin. I 
am facingchallenges in obtaining nice sections, and I was wondering if you 
could providesome guidance or recommendations.

Thank you in advance for your help!
Chakib
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[Histonet] We were introduced to an awesome new product!

[Cooper, Brian via Histonet]

Good afternoon HistoNet!

You know that product that many of us use to skim our flotation baths in 
between cutting blocks?  You know, those individual sheets in the green box?  
Well, for years now, I for one have been frustrated when say, a white-pages 
sized chunk of them come out when I go to grab one!  (I know, I dated myself 
there).  Or how the next sheet will retract inside the box, and then you have 
to surgically extract it to get on with it.  Or how this product tears, and 
you're forced to skim your bath with only a fragment?  It's been in every lab 
I've ever worked in!

If you get a chance to try them, Tech-Wipe specialty task wipers are such an 
amazing upgrade!  We have no financial gain here, I'd never even heard of this 
product until a few weeks ago.  None of the issues I mentioned above have 
happened!  Every one of our crew has told me how much they love this new 
product!  These have a little bit of elasticity to them, and that seemingly 
prevents tears.  And they only come out one at a time!  Ask your consumables 
rep if they carry it!  I never thought I'd write an email about skimming a 
waterbath; it's such a mundane task we've done millions of times as Histotechs. 
 But here you go!

Happy Tuesday!

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Microwave Processing

[Cooper, Brian via Histonet]

They may make a combo UPS/line conditioner.  That would be ideal.  Line 
conditioners stabilize and protect against power spikes/surges.

Thanks, 

Brian

-Original Message-
From: Campbell, Tasha  
Sent: Wednesday, November 8, 2023 12:40 PM
To: Cooper, Brian 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave Processing (EXTERNAL EMAIL)

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What does a line conditioner do? The company was bought out recently and 
basically has no employees so I have no one to help me. 

Tasha Campbell
Sent from my iPhone

> On Nov 8, 2023, at 3:36 PM, Cooper, Brian  wrote:
> 
> Hi Tasha,
> 
> Maybe look into getting a line conditioner for your tissue processors.  Your 
> tissue processor vendor may have some suggestions.
> 
> Thanks,
> 
> Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
> Pathology and Laboratory Medicine Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357
> bcoo...@chla.usc.edu
> 
> -Original Message-
> From: Campbell, Tasha via Histonet 
> Sent: Wednesday, November 8, 2023 11:44 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL)
> 
> CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
> CHLA. Do not open attachments, click on links, or respond unless you expected 
> this message and recognize the email address: 
> histonet-boun...@lists.utsouthwestern.edu.
> 
> Is there anyone that is super familiar with microwave processing? I really 
> need some help.   I am having trouble with my tissue and I think it's the 
> processing.  I don't know what else it would be.  We moved to a new building 
> so I don't know if the power could be stronger and its changing the way it 
> processes or what.  I have tried messing with the program times and temps but 
> I cannot figure it out.  My blocks keep having what looks like knife lines 
> but it's not the microtomes. The only change has been moving into a brand new 
> building.  Any help would be appreciated!  Thanks.
> 
> 
> Tasha Campbell, B.S., HTL (ASCP)
> Frederick Gastroenterology Associates
> 7109 Guilford Dr. Suite 300
> Frederick, MD 21704
> 301-695-6800 ext. 144
> 
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Re: [Histonet] Microwave Processing

[Cooper, Brian via Histonet]

Hi Tasha, 

Maybe look into getting a line conditioner for your tissue processors.  Your 
tissue processor vendor may have some suggestions.

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Campbell, Tasha via Histonet  
Sent: Wednesday, November 8, 2023 11:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL)

CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
CHLA. Do not open attachments, click on links, or respond unless you expected 
this message and recognize the email address: 
histonet-boun...@lists.utsouthwestern.edu.

Is there anyone that is super familiar with microwave processing? I really need 
some help.   I am having trouble with my tissue and I think it's the 
processing.  I don't know what else it would be.  We moved to a new building so 
I don't know if the power could be stronger and its changing the way it 
processes or what.  I have tried messing with the program times and temps but I 
cannot figure it out.  My blocks keep having what looks like knife lines but 
it's not the microtomes. The only change has been moving into a brand new 
building.  Any help would be appreciated!  Thanks.


Tasha Campbell, B.S., HTL (ASCP)
Frederick Gastroenterology Associates
7109 Guilford Dr. Suite 300
Frederick, MD 21704
301-695-6800 ext. 144

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Re: [Histonet] Formalin pH

[Cooper, Brian via Histonet]

Ask your vendor to send a certificate of analysis that includes the pH. That's 
what we did and now they come with each lot. We do have to bug them for them 
occasionally.

I'm betting your vendor has at least heard about this from other customers so 
it should be on their radar at the very least.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357

bcoo...@chla.usc.edu


From: Paula Sicurello via Histonet 
Sent: Tuesday, September 12, 2023 7:12:05 PM
To: HistoNet 
Subject: [Histonet] Formalin pH (EXTERNAL EMAIL)

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Hello Histoteckies,
What are y'all doing regarding the CAP requirement to monitor the pH of 
formalin?
We buy tons and tons of the 5 gallon cubitainers and we are still debating over 
how to check the pH.

Looking forward to your replies.
Toodles!
Sincerely,

Paula Sicurello
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Re: [Histonet] Leica H 600

[Cooper, Brian via Histonet]

The Ventana HE600 is an awesome machine. You can't use xylene to remove the 
coverslips, you need to heat them on a hot plate to soften the glue, and then 
the slips just come right off.

I used other dip and dunk stainers in the past and I was skeptical at first 
too. Those concerns have been put to rest! The machine requires no manual user 
maintenance (PM service is of course required) or filtering of reagents. You 
can't put expired reagents on the machine, nor can you put reagents in the 
wrong place. The machine performs a cleaning cycle every evening, so we 
literally do nothing on this machine except press start.

No machine is completely perfect. Sometimes staining modules leak or fail. This 
machine has 3 separate staining modules so you can always disable the troubled 
module and the machine will still work. It's been very rare that our 
coverslipper has failed.

It's certainly not the cheapest stainer on the market, but I've told people who 
come to our lab that the machine is like another employee! It just works and 
works and works. I'll be happy to answer any further questions.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357

bcoo...@chla.usc.edu


From: O'Neil, Beth via Histonet 
Sent: Wednesday, August 23, 2023 5:28:11 AM
To: Histonet 
Subject: [Histonet] Leica H 600 (EXTERNAL EMAIL)

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I would appreciate any feedback from anyone with experience using the Leica H 
600. I recently attended a demo of the unit and we are still hung up on the 
large slide tray it uses instead of the traditional slide racks.   I also spoke 
with another user who indicated that if you need to re-coverslip a slide it is 
very difficult to remove the existing coverslip and when you do the hematoxylin 
is removed from the stain ???  We already know we will need two units to handle 
our workload of approximately 900 H  Any information would be greatly 
appreciated.

Beth O'Neil
beth.one...@wvumedicine.org
West Virginia University, JW Ruby Memorial Hospital
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Re: [Histonet] Tissue Processor Schedule Validations

[Cooper, Brian via Histonet]

WOW!  I came back from lunch and I have seven responses already!  Everyone said 
the same thing, which was my game plan, take punches from larger samples!  
Happy Friday Eve everyone!   

Thanks so much Histonet!  

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 11:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations (EXTERNAL EMAIL)

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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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[Histonet] Tissue Processor Schedule Validations

[Cooper, Brian via Histonet]

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tracking of blocks onto processors

[Cooper, Brian via Histonet]

Hi Chris, 

At our institution, we're currently utilizing CoPath Automatic Barcoding and 
Tracking (AB).  Similar to Vantage, each step of the workload is tracked from 
accessioning → grossing → embedding→ microtomy→ staining→ QC (or case assembly) 
and then delivery to pathologist. For every cassette that grossed by our PA's 
the day before, that information shows up on an automatically printed report 
for our techs to verify that the actual block exists the next day (when they 
are embedded and cut) or being accounted for at the time of filing.  At 10 AM, 
a 2nd report will print highlighting any block that has yet to be cut (these 
blocks will either be in the grossed or embedded status).  Usually, this final 
report is simply a blank page, because we typically have all of our routine 
workload cut before this time.  If anything IS present on this report, it 
indicates that there is a cassette we didn't embed, have yet to cut, or worst 
case scenario, lost.  This report is basically a red flag warning--it's a way 
to tell if anything is missing, and that you'd better locate that missing 
sample ASAP.

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Willis, Donna G via Histonet  
Sent: Wednesday, March 22, 2023 5:29 AM
To: Hagon, Christopher (Health) ; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tracking of blocks onto processors (EXTERNAL EMAIL)

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Chris,
We have been tracking blocks since we went live with Vantage over 10 years ago. 
 Roche (Ventana then) built a custom program for us to use at that time because 
they had not yet implemented it into Vantage.  In the past 2 years Roche has 
updated their software to allow documenting this information.  There are 
several options of how the scanning is performed.  For our lab we scan before 
we place the block into the formalin holding tanks, right after the 
PA/Resident/Grossing Tech have placed the tissue into the block.  I prefer it 
done there because I do not want my technicians exposed an extra time to 
formalin if the scanning is done right before the baskets are placed on the 
processors.  We have a manual log that is used to document the time the 
cassette are placed in and taken out of the processors.  We also track our 
blocks and slides into storage.  I hope this helps.  

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, March 21, 2023 5:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Tracking of blocks onto processors


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UNOFFICIAL

Hi Histonetters,

Just wondering if or how others are tracking/scanning racks of blocks onto 
processors. In this day and age of traceability with barcodes, we scan each 
block in each rack before loading onto a processor and recording which 
processor that rack went on to. Has anyone got a more efficient way of doing 
this? Assigning at grossing/cut-up is an option, but is an extra step for the 
PA/registrar and relies on knowing which processor is in use that day.

Looking in the crystal ball, will next gen processors automatically scan blocks 
as the first step? Load blocks, start the run and it produces a report of the 
blocks on that run? Interested to hear peoples thoughts.

Cheers,

Chris Hagon - Pathology
Digital Solutions Division, ACT Health Directorate


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Re: [Histonet] Special Stainers

[Cooper, Brian via Histonet]

Agilent-Artisan stainers are amazing.  You can search old Histonet posts with 
my explanation as to why! I once had a lot to say about this. 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Kara, Phillip via Histonet  
Sent: Wednesday, February 22, 2023 11:05 AM
To: Eddie Martin via Histonet 
Subject: [Histonet] Special Stainers (EXTERNAL EMAIL)

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So I am working for a brand new lab that has been open since September. We 
currently perform all our specials by hand (roughly 7 stains; 3 silvers) and 
our numbers are starting to shoot up big time on them.
Any recommendations on special stainers and kits that go with them? We are a 
Sakura flag ship lab but we are open to other equipment if the cost is good and 
the results are great.

Phillip Kara BS, HTL(ASCP) : Lead Histology Tech Wise Pathology LLC
609 Medical Center Dr.
Decatur TX. 76234
Office: 940-626-0120
pk...@wisehealthsystems.com

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Re: [Histonet] destain for IHC

[Cooper, Brian via Histonet]

We used to do this all the time in my old reference lab.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357

bcoo...@chla.usc.edu


From: Nancy Schmitt via Histonet 
Sent: Wednesday, February 8, 2023 9:29:13 AM
To: Histonet 
Subject: [Histonet] destain for IHC (EXTERNAL EMAIL)

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Hello-

Has anyone had success destaining an H and then running IHC on the same slide?

Thanks!

Nancy


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Re: [Histonet] CAP regulatory question

[Cooper, Brian via Histonet]

Hi Jessica, 

That's the way we read it here, but in most cases we have probably more like 3 
years-worth. 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

Thanks, 

Brian

-Original Message-
From: Piche, Jessica via Histonet  
Sent: Thursday, February 2, 2023 9:13 AM
To: Histonet 
Subject: [Histonet] CAP regulatory question (EXTERNAL EMAIL)

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Good Afternoon,

Do we only have to keep lot to lot comparisons for immunohistochemistry and 
special stains for 2 years?

I hope everyone is having a good day.

Thank you.

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
Histology Team Leader
203-573-7167
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Re: [Histonet] Temperature Monitoring System

[Cooper, Brian via Histonet]

We use Isensix here, and I really like the system.  It's not exactly "wireless" 
because each unit is hardwired (with AA battery backup) into a CP unit which 
transmits relays data back to an Access Point (also hard wired), which in turn 
relays the information to the Control Center software. When a unit goes out of 
range, the system can email designated individuals or page specific numbers, 
and has backup notifications to designated individuals if the primary contact 
doesn't respond within a designated number of notifications sent.   They are 
"wireless" in the sense that there aren't wires running through ceilings back 
to a single central location, but there are certainly visible wires wherever a 
CP or AP is located.  We monitor refrigerators, freezers, room temp and 
humidity in countless sections of the pathology lab, and even elsewhere in the 
hospital here.  

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Matthew D. Roark via Histonet  
Sent: Wednesday, January 18, 2023 8:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature Monitoring System (EXTERNAL EMAIL)

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Good morning all!

Is there a wireless temperature monitoring system that you would recommend?





Matthew Roark, HTL(ASCP)CM
Histology Specialist
Laboratory

P 573-331-3982 | M 573-979-1925 | F 573-331-5049 
mro...@sfmc.net

Saint Francis Healthcare System
211 Saint Francis Drive
Cape Girardeau, MO 63703

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

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Re: [Histonet] Cassette printers

[Cooper, Brian via Histonet]

Good Morning Paula, 

All I've ever used is the Leica IPC Cassette printer across my last few 
institutions.  They are absolute workhorses; printing is fast and beautiful and 
the maintenance is really easy.  The only downsides I can think of is that the 
IPC has a large footprint, and the ink is really pricey.  You're supposed to 
change the cartridge every 3.5 months or 60,000 prints or so.  We never hit 
that volume so we're always throwing away ink! 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Paula Sicurello via Histonet  
Sent: Wednesday, December 28, 2022 10:44 AM
To: HistoNet 
Subject: [Histonet] Cassette printers (EXTERNAL EMAIL)

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Good Morning Listers,
We are in the market for a new cassette printer.  Has anyone used the General 
Data LaserTrack FLEX system?  I'm interested in how well it actually prints on 
3 sides of the cassettes.
Any input about any other vendors printers is helpful.  I have quotes from 
General Data, Sakura, and Leica.
Please provide your opinions and related experiences with each of the vendor's 
cassette printer.  We currently have a General Data CL12 that is dying a slow, 
painful death.
Thanks oodles for your help.

Paula Sicurello
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[Histonet] Cryogenic Vials?

[Cooper, Brian via Histonet]

Good afternoon Histonet,

What type of containers are you using for storage of frozen tissue samples 
collected in the gross room?  Our current cryogenic vials have been on 
backorder for more than a year now, and they keep pushing back our delivery 
date.

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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[Histonet] Epon Resin Embedding Question

[Cooper, Brian via Histonet]

Good afternoon Histonet,

Asking this question for a friend:  Is there a way to correct an improperly 
prepared Epon resin embedded block?   Following embedding, the block is soft 
and "tacky" to the touch.  This block is proving VERY difficult to section.  
Any assistance from those of you doing EM in your labs would be greatly 
appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Molds- cold vs warm

[Cooper, Brian via Histonet]

Thanks for saying this Jay!! I have to say, it's been a while since we've had 
such a great response on Histonet!! Everything you said is spot on.

Happy Friday everyone!

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles
Sent from my mobile





On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet 
 wrote:
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Whoever is telling you to use cold molds needs to go back to clown college.

That is totally, 100%, absolutely, wrong.

There is some debate as to embed "wet" (cassettes submerged in paraffin
bath) or "dry", and I will accept either, as mostly a matter of personal
preference.  BUT, in both of these cases, the molds are hot.

I have been a Histotech for five decades, trained at Armed Forces Institute
of Pathology (back when that used to mean something) and I have NEVER seen
anyone using cold molds.

It is a guaranteed way to get cold fractures and cracks in your blocks, or
to pop tissue out when you are cutting, which might be irretrievable. Just
think how much time all those re-embedded blocks are going to save you!

Also, you won't be able to easily re-position specimens in the block, to
put them "on edge" or "on end", for example.  The tissue will instantly
stick to the cold mold.  And if you want to re-position it, guess what,
you'll have to warm the mold up to get the tissue unstuck.  How's that
(non-existent anyway) time savings now?


If you want to prove to whatever jackass suggested this that they are
wrong, get a big stack of every histopathology textbook you can find.
There is nothing in any of them talking about paraffin embedding with cold
molds.

As a matter of fact, every single textbook will specify molds at the same
temp as paraffin.

Anyway, it doesn't even make sense, thermodynamically.  Heat travels from
hot to cold.  Those "cold" molds will be the same temperature as the
paraffin, almost instantly. Did it take a tiny amount of heat out of the
hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The
amount of heat from the paraffin used to warm the mold is trivial compared
to the total heat of the system. That's why cold plates have huge, noisy
refrigeration units.  You can't argue with thermodynamics.

If you are having trouble getting your blocks to release, use mold
release!  Viola!  
https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com
 I used to think it was
superfluous, but now I consider it compulsory.  This is probably the answer
to most of your issues.

I don't know who is suggesting using cold molds, but I can pretty much
guarantee that it's a pathologist who thinks his slides are taking too
long, and knows nothing about histopathology, or a lab manager, who knows
nothing about histopathology.  This next part is directly to them.

To Whoever Suggested Cold Molds:  The answer to getting your slides out
quicker is buying more equipment and hiring more techs, and holding
everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding).
Making nonsensical, uninformed suggestions only exposes your ignorance.

Please feel free to show them this reply.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)
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Re: [Histonet] Automated ihc staining of bone

[Cooper, Brian via Histonet]

Charles, 

Try and blot your sections dry before placing them into the oven.  After 
picking up your section, place your slide onto a flat surface and take a paper 
towel or piece of filter paper, and gently press it down on top of the section 
to wick away all of the moisture.  We use this technique frequently here on 
bone marrow cores.

Thanks, 

Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Charles Riley via Histonet  
Sent: Tuesday, August 30, 2022 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL)

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I am working on trying to get IHC stains optimized on bone samples of rat 
tibias. The issue I am running into is that the periosteum is separating from 
the bone marrow and wrinkling up over top of the rest of the sample.

If anyone has any techniques to prevent this wrinkling/detachment of tissue 
from the main section during automated ihc staining it would be greatly 
appreciated.
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Re: [Histonet] Slide Printer

[Cooper, Brian via Histonet]

Similar story here, except we went with SlideMates.  Before AB LIS 
integration, we were able to have CoPath print a barcode on our cassettes 
(Leica IPC cassette printer). The SlideMate Field Service Reps were able to 
translate that barcode info on the slide printers (which were still standalone 
at that point).  That barcode on the cassettes translated to a patient name and 
accession number on each slide.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Terri Braud via Histonet  
Sent: Friday, August 5, 2022 12:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide Printer (EXTERNAL EMAIL)

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We've been using our Leica IPS and IPC printers for over 15 years, first as 
stand-alone units, and now, integrated into CoPath.  What a workhorse these 
instruments this have been!  Their modern equivalent is almost identical, so 
parts are readily available.  My recommendation would be to repair or replace 
with the same. 

Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
  Honesty
AccouNtability
    AgiLity
    CoLlaboration
  CoMpassion

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, August 5, 2022 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4

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Today's Topics:

   1. slide printers (Colleen Forster)


--

Message: 1
Date: Thu, 4 Aug 2022 17:15:11 -0500
From: Colleen Forster 
To: histonet-request 
Subject: [Histonet] slide printers
Message-ID:

Content-Type: text/plain; charset="UTF-8"

HEllo Histonetters,

We are a research lab that has been using the Leica IPC/IPS printed for many 
years. Today the flash motor went out in the IPS. We are now looking into 
updating the slide printer.

We are research only and NOT hooked into any LIS system. It would be a stand 
alone process. Can some of you share what you have and the pros/cons?
I need to start the search for the replacement I would like.

Thank you in advance.

--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930


--

Subject: Digest Footer

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End of Histonet Digest, Vol 225, Issue 4



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Re: [Histonet] HSV testing

[Cooper, Brian via Histonet]

We run Cell Marque's HSV1 and HSV2 on our Leica Bond platform here.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Nancy Schmitt via Histonet  
Sent: Wednesday, July 6, 2022 4:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HSV testing (EXTERNAL EMAIL)

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Hello-
What are you using for HSV IHC testing?
Thank you
Nancy

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Re: [Histonet] microtome pm

[Cooper, Brian via Histonet]

Once a year is good for us.  Just an added thought: we always make sure they 
change the extension springs in the cassette clamp with each PM as well.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Paula via Histonet  
Sent: Tuesday, June 28, 2022 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome pm (EXTERNAL EMAIL)

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Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6 
months but I'm thinking once a year is sufficient and I wanted to get some 
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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Re: [Histonet] Melanoma Control Blocks?

[Cooper, Brian via Histonet]

Good morning Histonet!  

Just wanted to let you know that we've received a lot of offers for help on 
melanoma controls since my email yesterday.  Thank you to all who have 
responded. I think we will likely have melanoma controls for many years to come 
if these all pan out!  Much appreciated Histonet; when one of us needs help, 
this is always such a valuable resource.   Happy almost Friday!  

Thanks, 

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Wednesday, March 30, 2022 10:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Melanoma Control Blocks? (EXTERNAL EMAIL)

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Good morning Histonet!

Does any of you have any melanoma control blocks you'd be willing to share?  We 
have a bunch of other controls we can trade for: CMV, EBV, Pneumocystis, HSV 1 
or HSV2 just to name a few.  Please message me offline if you're able to help!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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[Histonet] Melanoma Control Blocks?

[Cooper, Brian via Histonet]

Good morning Histonet!

Does any of you have any melanoma control blocks you'd be willing to share?  We 
have a bunch of other controls we can trade for: CMV, EBV, Pneumocystis, HSV 1 
or HSV2 just to name a few.  Please message me offline if you're able to help!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] best paraffin

[Cooper, Brian via Histonet]

Paraplast for processing and Paraplast Xtra for microtomy at our institution.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Jay Lundgren via Histonet  
Sent: Thursday, March 10, 2022 9:22 AM
To: John Garratt ; Rinker, Jeffrey 
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] best paraffin (EXTERNAL EMAIL)

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Paraplast plus is my personal favorite.

On March 10, 2022, at 11:04 AM, John Garratt via Histonet 
 wrote:

The paraffins available from lab suppliers (not candle makers) is all of 
excellent quality these days. There is some variability, but choice of paraffin 
usually comes down to personal preference.
If you are having problems cutting it may well be related to other factors like 
fixation and processing, and of course, the microtomy.
What are the issues you are encountering?

John
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On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet 
 wrote:

> I was wondering what people think is the best paraffin to use. I find it hard 
> to get a good read on what is best for our lab. We do around 60-100 blocks a 
> day and do a bx and surgical run overnight. I think that we are having 
> problems with cutting because we are not optimized and i am trying to fix 
> that.
> --
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Re: [Histonet] contents of Histonet digest

[Cooper, Brian via Histonet]

Our formalin vendor is sending us certificates of analysis with every shipment 
now. We review these forms to ensure the lot is acceptable and file them in a 
binder.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles
Sent from my mobile

On Mar 4, 2022 7:18 AM, "Maimone-Schoen, Michele via Histonet" 
 wrote:
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respond unless you expected this message and recognize the email address: 
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How are labs addressing the new CAP standard ANP.10041: Quality of Formalin 
Monitoring?   Thank you.


Michele Maimone-Schoen, MS
Manager, Anatomic Pathology
New York-Presbyterian Hospital
525 East 68th Street, Starr 1003
New York, NY  10065
212-746-2633 (Office)
212-746-5007 (Fax)
646-856-1738 (Cell)

Be Amazing!

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[Histonet] Stat6 control material

[Cooper, Brian via Histonet]

Good afternoon Histonet,

Do any of you have any STAT6 control material available to share? Please 
message me offline if you're able to help; we have lots of control materials 
that we can trade.

Thanks,

Brian Cooper
Histology Supervisor, Children's Hospital Los Angeles
bcoo...@chla.usc.edu
Sent from my mobile phone
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[Histonet] Histochemical autostainer

[Cooper, Brian via Histonet]

Dako (Agilent) Artisan is the way to go.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles

Sent from my cell phone, so please excuse any funny typos.

On Jan 26, 2020 5:47 PM, "Azam, Muhammad via Histonet" 
 wrote:
Hi all:

Our special stainer went kaput last week. Anybody would recommend a reliable 
benchtop autostainer for histochemical stains.

V/r

Muhammad Azam, MD
Chief, Pathology & Laboratory Medicine Service (113)
James H. Quillen VAMC
Mountain Home, TN
Phone: 423-979-3529
Cell: 423-741-9872

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[Histonet] Deep Water Bath with Lighting

[Cooper, Brian via Histonet]

I really like the TFB 35 flotation baths from Medite. They make a shallow and a 
deeper dish version; you'll want to go with the deeper dish. I'm not in front 
of one right now, but I suspect they're about 3" give or take.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles

Sent from my cell phone, so please excuse typos


On Dec 18, 2019 3:45 PM, Sandra Cheasty via Histonet 
 wrote:
Ho-ho-ho all,
I'm looking for a deep water bath, (3" minimum), that has an 
interior light source. Most of them seem to be only 2" tall, or have a slanted 
front control panel that none of us in the lab want. Does anyone know of a 
brand of flotation water baths that are 3" deep and have interior lighting?
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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[Histonet] Cryostat Samples for Molecular Testing

[Cooper, Brian via Histonet]

Good afternoon Histonet!

We would like to know what decontamination procedures you are performing to 
prevent cross-contamination in between cutting scrolls for frozen samples for 
molecular testing.  We've had a few complaints of trace carryover from one 
sample to the next.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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[Histonet] TJP2 antibody

[Cooper, Brian via Histonet]

Good morning Histonet,

Is anyone out there working with TJP2 (tight junction protein) antibody?  If 
so, we'd love to hear from you, and ideally, get a slide or two that 
demonstrates loss of this protein in human liver.  We're willing to provide you 
with a control block for something else if you can help us out.  Pediatric 
institutions-this one may be in your wheelhouse!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Sectioning over processed GI biopsy

[Cooper, Brian via Histonet]

Did you try soaking them face-down on your flotation bath for 20 seconds or so, 
prior to placing on a very wet ice bath (over a Labsorb or paper towel)?  It 
sounds like these GI's were processed with an inappropriate protocol on your 
tissue processor.

Good luck.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 18, 2019 9:49 AM
To: Histo List
Subject: [Histonet] Sectioning over processed GI biopsy (EXTERNAL EMAIL)

What is the best way to get a section from an over-processed GI biopsy?
Paraffin keeps pulling away from the tissue and nothing sections. Tissue
very hard even after lenghty time on ice water bath

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] Mini Lab Incubators?

[Cooper, Brian via Histonet]

Good Afternoon Histonetters!

Does anyone out there have any recommendations as far as Mini Lab Incubators 
go?  Have looked at the VWR Digital Mini Incubator and LabNet International 
Mini Incubator online this morning.  Haven't seen much in the way of comments 
on these products; would love to hear your feedback.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] New Lab

[Cooper, Brian via Histonet]

Know what else you can't have enough of?  Data ports.  We just went live with 
barcoding and tracking and we had to add TONS of them in nearly every room!

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, January 11, 2019 10:28 AM
To: Campbell, Tasha M.
Cc: Histonet
Subject: Re: [Histonet] New Lab (EXTERNAL EMAIL)

Hi Tasha,

How fun!  All I can say is ventilation, ventilation, ventilation.  If you
can't get proper ventilation I recommend Sentry Air Systems.  They have
portable fume extractors that use a series of carbon filters, to suck up
the xylene or formaldehyde vapors.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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On Fri, Jan 11, 2019 at 5:37 AM Campbell, Tasha M. via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello, I am a small GI lab and our facility is moving to a brand new
> building!  The building has yet to be built so I have full control on how
> to design my lab.  I will have about 1000-1500 sq ft.  I was just wondering
> if anyone had any tips for me?  One thing I want to change is the type of
> flooring.  Right now we have the normal 12 x 12 in tiles that are built in
> most buildings.  Is there any other type of flooring that is better
> especially when dealing with the paraffin? And the fact that I am scraping
> the floors?   I use to work at the local hospital here and they remodeled
> the lab there and they put vinyl flooring in that is supposed to be made
> for labs.  Come to find out during the first xylene spill that xylene melts
> vinyl!  So I know I don't want to go that route.
>
>
> Thanks!!
>
>
>
>
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
>
>
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Re: [Histonet] Brachyury

[Cooper, Brian via Histonet]

We use the mouse monoclonal (1H9A2) from AbCam.  Works like a charm.

https://www.abcam.com/brachyury--bry-antibody-1h9a2-ab140661.html 

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Oler, April via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, December 26, 2018 6:20 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Brachyury (EXTERNAL EMAIL)

Good morning Histonet,

Has anyone had luck finding a Brachyury antibody that works well? After the 
Santa Cruz (H-210) was discontinued, I've been unable to find an antibody to 
replace the H-210. I've tried two antibodies (Santa Cruz D-10 and BioSB X1A02), 
but I was unable to validate either of them due to inconsistent staining 
results.

Thank you in advance for any information you can provide.

April Oler, HT
Lead Tech IHC/DIF/Muscle
Michigan Medicine
University of Michigan
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Re: [Histonet] Low Oxygen Sensor/Alarms

[Cooper, Brian via Histonet]

Hi,

Posting this question again to my colleagues on Histonet, as I have yet to 
receive a response.   In regards to newly revised LABGEN checklist,  GEN.77550 
states "In areas where liquid nitrogen is used, there are oxygen sensors with a 
low oxygen alarm mounted in an appropriate location and sufficient airflow to 
prevent asphyxiation."   For those of you who already do this, can you please 
tell me the type and model of sensor you're using?

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
bcoo...@chla.usc.edu


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[Histonet] Low Oxygen Sensor/Alarms

[Cooper, Brian via Histonet]

Hi,

Looking at the newly revised LABGEN checklist this afternoon.  GEN.77550 states 
"In areas where liquid nitrogen is used, there are oxygen sensors with a low 
oxygen alarm mounted in an appropriate location and sufficient airflow to 
prevent asphyxiation."   For those of you who already do this, can you please 
tell me the type and model of sensor you're using?

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
bcoo...@chla.usc.edu


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Re: [Histonet] Experience (especially long term)

[Cooper, Brian via Histonet]

I've used both.  Agree that the Sakura's are awesome; used them for years at 
another institution.  We currently have two of the Histostars, and we actually 
like the design a little better.  The Histostar's hot plates are set back a 
little further than those on the Sakura model.  In my case, this results in 
much fewer instances of "paraffin sleeves" on my lab coat!  The remaining 
plastic space in front of the hot plate is a great place to align GI biopsies 
in your mold during orientation, or to flatten out and scrape paper wrapped 
cell block material.   On our Histostar models (probably about 5 years old now) 
we don't have the ability to adjust the temperature of the cold plate.  I do 
miss that, because we've found ours too be a little too cold, and we sometimes 
get cracks in our paraffin blocks (this is REALLY inconvenient on needle 
biopsies). I believe that on newer models, this feature has been added.   

That's my two cents worth...

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Kristyn Ferber via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 27, 2018 10:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Experience (especially long term) (EXTERNAL EMAIL)

Hello Histonetters!

I'm looking to gather histotech's experience with Thermo Scientific's
HistoStar embedding workstation. I am very familiar with Sakura and think
it's top notch as far as service and support and, especially, longevity.
How does everyone feel the HistoStar compares with the Tissue Tek system if
you've used both?

Thanks in advance!

Kristyn Ferber, HTL
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Re: [Histonet] Luxol fast Blue Question

[Cooper, Brian via Histonet]

Hi Lisa, 

We keep them in solution at 56-60 degrees overnight, and typically start 
differentiation between the 15-20 hour range. 

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 


-Original Message-
From: Chapman, Lisa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, September 12, 2018 12:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luxol fast Blue Question (EXTERNAL EMAIL)

Hello All,

When performing a LFB for myelin and nissl, how long does everyone keep their 
slides in the LFB solution for and what temp do you use?  Most procedures say 
"overnight" and Sheehan states 16-24 hours.

Any advice would be greatly appreciated!  Thank you in advance!

Lisa Chapman HT (ASCP)
ACL Laboratories
Milwaukee, WI
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Re: [Histonet] tissue processor

[Cooper, Brian via Histonet]

We absolutely love our Leica Peloris II.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Lisa Brenner via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, February 02, 2018 10:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue processor (EXTERNAL EMAIL)

Hello,

   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?

Lisa Brenner


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[Histonet] Type of Nitrile Gloves in your lab

[Cooper, Brian via Histonet]

Hi,

Does anyone out there use a brand of nitrile glove that they really like?  The 
ones we use here are not so good.  When we pull them on, the part closet to the 
wrist often tears off, leaving behind a nice blue rubber band.  At least 
several gloves per box have tears in them before we even put them on.  One of 
our Quality Managers asked us to come up with alternatives.  Can you help me 
out?   You can email me offline if you don't want to name particular brands for 
everyone to see.  PLEASE HELP!!!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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Re: [Histonet] LFB-H controls

[Cooper, Brian via Histonet]

We use spinal cord.

-Original Message-
From: Diane Satterfield via Histonet [histonet@lists.utsouthwestern.edu]
Received: Friday, 03 Nov 2017, 7:39AM
To: 'histonet@lists.utsouthwestern.edu' [histonet@lists.utsouthwestern.edu]
Subject: [Histonet] LFB-H controls (EXTERNAL EMAIL)

I am getting ready to learn how to do the LFB-H staining on brain tissue.  I 
was wondering want I would use for a control with this staining.

Diane
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Re: [Histonet] Lab assistants

[Cooper, Brian via Histonet]

Too funny Jay!  I take it you've met my old boss!!  LOL! 

Brian

-Original Message-
From: Jay Lundgren via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, October 12, 2017 1:04 PM
To: Hannen, Valerie
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Lab assistants (EXTERNAL EMAIL)

The most important role of the lab assistant in the Histology laboratory is to 
be the target of middle management ire.

  Sincerely,

   Jay A. Lundgren, M.S., HTL(ASCP)

On Wed, Oct 11, 2017 at 4:00 AM, Hannen, Valerie via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Good Morning,   We are having a debate about what a Lab assistant can and
> can not do in the State of Florida.  So, those of you in Florida 
> please weigh in.  Can they make alcohol  dilutions and use those alcohols to:
>
> 1)  Change an automatic stainer
>
> 2)  Change the tissue processor
>
>
> What about recycling said alcohols?   Are they allowed to pour formalin
> into the processor wells? Can they fill small bottles with formalin to 
> be used by doctor's offices?  The list could go on and on... but I 
> will stop here.
>
> Thanks so much for any insight into this!
>
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
> ==
> "This email is intended solely for the use of the individual to whom 
> it is addressed and may contain information that is privileged, 
> confidential or otherwise exempt from disclosure under applicable law. 
> If the reader of this email is not the intended recipient or the 
> employee or agent responsible for delivering the message to the 
> intended recipient, you are hereby notified that any dissemination, 
> distribution, or copying of this communication is strictly prohibited. 
> If you have received this communication in error, please immediately 
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Re: [Histonet] What is the best microtome?

[Cooper, Brian via Histonet]

Microm HM325 gets my vote.

-Original Message-
From: Dawn Bugge via Histonet [histonet@lists.utsouthwestern.edu]
Received: Wednesday, 13 Sep 2017, 3:04PM
To: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: [Histonet] What is the best microtome? (EXTERNAL EMAIL)

Hello everyone,

What is your favorite microtome?  I am helping a lab with purchases and I
only have experience with the Finesse ME+ and an old Leica.  Any thoughts
would be great.

Thanks.

--
Dawn R Bugge
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[Histonet] Need help identifying a product!!!

[Cooper, Brian via Histonet]

Happy Friday Histonet!

Our cassette printed died this week, and we've been handwriting cassettes for 
the last couple of days.  It's been YEARS since I had to do this; BOY has my 
handwriting atrophied since then!  I remember we used to have this little metal 
rack that held the cassettes perfectly stationary when you wrote on them; the 
rack held about 20-25 cassettes in 4 or 5 rows.  Do any of you remember these?  
I've been searching online for a couple days, but Google and all the lab supply 
sites just aren't catching what I'm looking for.  Our printer will be fixed in 
a few hours, but just in case, I'd like to have one of these racks for the 
future.  Any ideas?  Should I check antique shops?  :)

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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Re: [Histonet] Leica SelecTech Stains

[Cooper, Brian via Histonet]

Hi Amanda, 

We used to use their Define Solution, but now we simply stain with their 
Hematoxylin 560 MX and Alcoholic Eosin-Y.  1% acid alcohol (5 quick dips) for 
differentiation and dilute ammonia water for bluing.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 

-Original Message-
From: Amanda Reichard via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, July 10, 2017 6:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica SelecTech Stains (EXTERNAL EMAIL)

For those of you using the SelecTech stains...

Do you use the entire system (define and blue buffer), or just the hematoxylin 
and eosin?

It seems from previous posts everyone who has used it is very happy with it, 
I'm just curious if we can stick with a less pricey differentiation and bluing 
solution.

Thanks in advance

Amanda Reichard, HTL (ASCP)cm
Histology/Cytology Supervisor
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
(220) 564-4163
areich...@lmhealth.org




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[Histonet] Empty Chemical Containers

[Cooper, Brian via Histonet]

Happy Lab Week Histonet!

How are you all disposing of empty chemical containers?   Triple rinsing and 
throwing them in regular trash (with the labels defaced,) or having the empty 
containers disposed of by your outside chemical contractors? I'm sure this 
answer will vary from state to state, and institution to institution (and 
chemical to chemical for that matter).  Sorry if this has been asked 
previously-I searched the archives, and an answer wasn't readily found.  This 
came up during safety rounds the other day, and our institution's policy on 
this is pretty vague.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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Re: [Histonet] BRAF V600E

[Cooper, Brian via Histonet]

Thank you to all who replied to my inquiry about BRAF on the Bond Max.  As 
always, I'm truly grateful for the valuable resource that is Histonet. 

Happy Holidays everyone!

Brian

-Original Message-
From: Cooper, Brian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, December 13, 2016 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BRAF V600E

Good Afternoon Histonet!

It's been a few years since I posted this question initially; thought I'd give 
it a try again.  Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or 
Spring Bioscience's) antibody on a Leica Bond Max?  If you are, I'd really 
appreciate hearing how you did it.  This antibody is quite expensive, and we 
don't particularly want to burn through massive amounts of money trying to 
reinvent the wheel.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>



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[Histonet] BRAF V600E

[Cooper, Brian via Histonet]

Good Afternoon Histonet!

It's been a few years since I posted this question initially; thought I'd give 
it a try again.  Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or 
Spring Bioscience's) antibody on a Leica Bond Max?  If you are, I'd really 
appreciate hearing how you did it.  This antibody is quite expensive, and we 
don't particularly want to burn through massive amounts of money trying to 
reinvent the wheel.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu



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Re: [Histonet] On the hunt for new microtomes!

[Cooper, Brian via Histonet]

We've used Microm microtomes for years and they have always been 100% trouble 
free.

-Original Message-
From: Jennifer MacDonald via Histonet [histonet@lists.utsouthwestern.edu]
Received: Monday, 01 Aug 2016, 12:45PM
To: Rene J Buesa [rjbu...@yahoo.com]
CC: Mary Faith Encarnacion [mfb.encarnac...@gmail.com]; 
histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: Re: [Histonet] On the hunt for new microtomes!

Lecia makes the Sakura microtome.  We have both Sakura and Microm
microtomes in our student lab.  They have both proved to be reliable.
There is a difference between a Shandon microtome and a Microm microtome.



From:   Rene J Buesa via Histonet 
To: Mary Faith Encarnacion ,
"histonet@lists.utsouthwestern.edu" 
Date:   08/01/2016 12:42 PM
Subject:Re: [Histonet] On the hunt for new microtomes!



I don't know now, but some years ago Thermo instruments were less that
reliable. Try Leica or even better Sakura.René

On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet
 wrote:


 Hey HistoNet,

Thanks to everyone who helped me out by providing their opinions on
embedding centers.  This time, I need everyone's thoughts on microtomes.
My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
Your thoughts and advice are very much appreciated!  If there are any more
I should try, let me know!

Thanks again,
Mary Faith
Histology Supervisor
VA Palo Alto Health Care System
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Re: [Histonet] block scrapers

[Cooper, Brian via Histonet]

I bought everyone really old pocket knives from flea markets--basically the 
same thing as what Jay said.  For the most part, these old knives were already 
really dull and didn't require any assistance to dull them further.

Good luck.

Brian


-Original Message-
From: Jay Lundgren via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 21, 2016 12:04 PM
To: Lauren Sweeney
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] block scrapers

Buy cheap paring knives at the dollar store and blunt the edges?

On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of 
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
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Re: [Histonet] Leica ASP6025 Tissue Processor and Cryostats

[Cooper, Brian via Histonet]

So we actually have a Leica CM1950 in our Histo Lab, as well as a Fisher NX70 
Cryostar in our Frozen Section Room in the OR.  They are both excellent 
cryostats (I actually learned how to cryosection on the Leica).  When we were 
in the market for a new Cryostat a year ago, we went with the NX70 because of 
the ergonomic flexibility and cold disinfection--both really cool (ok, pun 
truly wasn't intended there) features!  Check em both out, and see all the cool 
bells and whistles.

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Fortin, Joyce via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, March 10, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats

Could anyone who is using the Leica ASP6025 Tissue Processor please send me 
their opinions of it-pros and cons?
Also, which of the Leica cryostats you like the most?  We want the vacuum and 
UVC disinfection models.  Pros and cons...
Thank you!



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Re: [Histonet] Microtomes

[Cooper, Brian via Histonet]

This has been a Histonet post in the past; techs are fiercely loyal to their 
favorite brand and I'm sure you'll see people disagree with me as soon as I hit 
send!

I prefer Microm.  In our experience, block alignment is easier with the 
Microm's because the X and Y axis orientation knobs are positioned in such a 
manner that you can adjust block orientation with your left hand while your 
right hand is on the flywheel.  This allows you to keep a constant sight line 
of the entire depth of the block face in relation to the knife.  With the Leica 
microtomes, the positions of these screws are moved to the right, and the 
orientation of the Y axis requires you to either use two hands at once, or for 
you to move your left arm across your sight line to orient the Y axis.  While 
certainly not insurmountable; this just makes block orientation take a little 
longer.  Since we frequently have to recut blocks from different institutions, 
this is a big deal here.  I'm sure the peeps from the other camp are going to 
say that it's all in the technique.  To that I say, YEAH, YEAH, YEAH  :)

Both of these machines are well designed and will provide years and years of 
excellent service as long as they are well maintained.  The best thing you can 
do is ask to demo both machines and see what you like best. 

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: HERRINGTON, SHEILA via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 27, 2016 11:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtomes

We are looking to purchase two new microtomes and were wondering on thoughts 
and recommendations from the experience of the people that actually use them.  
Pros and cons would be extremely valuable on ergonomics, reliability and 
overall performance.

Sheila Herrington, RT
Technical Lead, Immunohistochemistry and Histopathology Kelowna General Hospital
2268 Pandosy Street,
Kelowna, B.C. V1Y 1T2

250-862-4300 x 7510

sheila.herring...@interiorhealth.ca



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Re: [Histonet] Alliance Rubber Bands

[Cooper, Brian via Histonet]

Awesome post Brent!   I thought I was the only one who had a strong opinion 
about rubber bands! I never used the orange color bands you mentioned, but the 
Alliance Pale Crepe Gold #64 were pretty awesome too.  Agree totally--cheap 
rubber bands HURT

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Brent Adams via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 27, 2016 11:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alliance Rubber Bands

Hey Histonetters,



I don't endorse products but this one is really good.



I don't get a kick back and no relatives work for the company, I just wanted

to give you all a good product to use if you are looking. I looked up the 
company

and found they are the last U.S. Made Rubber Band company and based out of

Arkansas.



I think I have had 2 eye injuries and multiple painful mishaps from

rubber bands that break far to easily so from that perspective I feel

I can give this product a thumbs up.



For the last 4 years I have been using  Alliance Rubber Company

non- latex rubber bands. The size 64 (3.5 x 1/4) work best on Slide folders

I have found. The Orange color rubber bands are easy to see and

hold the file folders together great and they rarely break.



I know it sound silly to talk about rubber bands this way but I was

really getting tired of being snapped by faulty rubber bands when trying to

wrap slide folders or paperwork.



I just had my first rubber band by Alliance snap a couple months ago which 
caught me

off guard cause it had been so long since that happened. It was then I look to

see what brand we were using and it turned out that we had been using the same

NON-latex rubber bands from the Alliance Rubber Company since I opened the 
Laboratory in 2012.

Before that at other labs breaking rubber bands were just part of the daily Job.



I think purchasing has gotten their best price on Amazon but they also use 
staples and

Office depot for supplies. I informed them that I only want these rubber bands 
and they

can get their best pricing where ever.



My fingers hurt a lot less after distributing slide folders and I don't have to 
swear as much. Ha Ha.

Funny how little things can really bother us.



FYI



Brent Adams - BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476
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Re: [Histonet] Best Glass Coverslipper?

[Cooper, Brian via Histonet]

Thank you to all who responded to my inquiry about the best glass coverslipper! 
 Everyone loves their Leica CV5030's and Sakura Glas G2's!

Thanks, 

Brian  


-Original Message-
From: Cooper, Brian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, January 21, 2016 2:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Best Glass Coverslipper?

Hey Histonet!

What's the best automatic glass coverslipper out there?  I only have experience 
with the Sakura tape slipper, which is AWESOME, but alas, we can't get one 
here.  Space is definitely an issue; would love to hear your experiences!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>



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[Histonet] Best Glass Coverslipper?

[Cooper, Brian via Histonet]

Hey Histonet!

What's the best automatic glass coverslipper out there?  I only have experience 
with the Sakura tape slipper, which is AWESOME, but alas, we can't get one 
here.  Space is definitely an issue; would love to hear your experiences!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] Document Control Systems

[Cooper, Brian via Histonet]

Happy Friday Histonetters!

For any of you out there using Title 21, Medialab or iPassport for document 
control (or anything else for that matter),  I would love to hear your feedback 
on what it's like to use the systems.  Pros/cons/responsiveness of the 
companies to your concerns-anything at all!  Your feedback would be greatly 
appreciated!  You can email me offline if you prefer.  We've seen demos from 
all three vendors, but would absolutely love to hear from people who actually 
use these systems.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] PFIC Case Material

[Cooper, Brian via Histonet]

Good evening Histonet,
Would any of you have access to, and be willing to share either blocks, or 
unstained slides from patients known to have progressive familial intrahepatic 
cholestasis (PFIC2 or PFIC3)?  We're interested in working up a few antibodies, 
yet a sufficient number of positive cases is proving to be elusive.  I'm 
willing to trade for control blocks that may have in our library.  Please 
contact me if you can help.
Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] Spirochete Control Block?

[Cooper, Brian via Histonet]

Good afternoon Histonetters!

I'm fairly certain my chances of seeing a puppy dog, riding on the back of a 
unicorn, sitting under a blue moon are greater, but does anyone have a block of 
spirochetes they'd be willing to share? Recently, we've gotten some great 
controls from these posts and thought we'd give this a shot!  We're more than 
willing to trade!  Please contact me directly if you don't want to post your 
responses.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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Re: [Histonet] CMV antibody clone

[Cooper, Brian via Histonet]

Thank you to everyone who replied to this post.  In case you're wondering, I 
got like 8 responses today, and everyone who responded uses CMV (DDG9/CCH2) 
from either Cell Marque or Dako.

You guys are the best!

Thanks,

Brian

-Original Message-
From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] 
Sent: Monday, July 13, 2015 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CMV antibody clone

Good afternoon Histonet,

Just taking a quick poll to find out which CMV clone most folks are running in 
their labs.  Your feedback is much appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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