Hi Bernard,

What is TD and TZ? MassLynx doesn't provide centroiding capability so 
MassWolf has a custom centroiding algorithm. I haven't seen any 
comprehensive comparisons between that custom algorithm and the MaxEnt 
algorithm available from ProteinLynx Global Server (which is not 
programmatically accessible even if you have access to PLGS!).

-Matt


Bernt wrote:
> Hi Natalie,
>
> I'm also currently trying out MassWolf 4.2.1......it works with TD and
> TZ as well, although TD appears to be a better option at generating
> smaller mzxml files. But the centroiding function doesn't seem to be
> working. It gives a "Warning: using EXPERIMENTAL centroiding
> functionality" error msg in command prompt and Windows pops up a
> "masswolf.exe has encountered a problem and needs to close" error
> report. Is the centroiding function still in the process of
> development?
>
>
> Bernard
>
>
> On Jun 25, 5:46 am, Natalie Tasman <[email protected]>
> wrote:
>   
>> Hello,
>>
>> First, you might want to check that  the file extention is indeed .raw  
>> and not .RAW.  Capitalization might make a difference.
>>
>> Can you open this file in the Waters MassLynx application on the same  
>> computer?  This must be installed in order for the converter  
>> (massWolf) to work properly.
>>
>> Natalie
>>
>> On Jun 24, 2009, at 2:39 PM, Hong wrote:
>>
>>
>>
>>     
>>> Hi there,
>>>       
>>> I am new to TPP. I have trouble converting the MassLynx .RAW directory
>>> with mzXML. I got the following status report message. What do I need
>>> to change to get it work? I greatly appreciate your inputs.
>>>       
>>> Hong Li
>>> NJ Med School
>>>       
>>> massWolf -v --mzXML c:/Inetpub/wwwroot/ISB/data/hilo08.raw
>>>       
>>> Settings:
>>>  centroid scans: 0
>>>  compress scans: 0
>>>  verbose mode: 1
>>>  lockspray: 1
>>>  MS^E: 0
>>>  threshold: 0
>>>  mzML mode: 0
>>>  mzXML mode: 1
>>>  input filename: c:/Inetpub/wwwroot/ISB/data/hilo08.raw
>>>  output filename: c:/Inetpub/wwwroot/ISB/data/hilo08.mzXML
>>>  output file as gzip: 0
>>>       
>>> Command FAILED
>>>       
>
> >
>
>   

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