The file is Micromass data. It is not odd for the size to read 0K, this is some sort of artifact of it being a .raw folder. The file is not corrupt, it opens on MassLynx properly, and can be run through PLGS with no troubles to a pkl file. It was acquired in MassLynx 4.1 however, might this make a difference as the user is using an older version?
On Jun 25, 2:36 pm, Natalie Tasman <[email protected]> wrote: > Thanks, Dave, and just pointing out that there is a description of > these options in the usage statement (printed out when running the > program with no arguments). > > Natalie > > On Jun 25, 2009, at 9:36 AM, Dave Trudgian wrote: > > > > > Matt, > > > TD filters out data points less than a given intensity, discarding > > them. > > TZ sets them to zero. You can producecentroidspectra using the > > Accurate Mass Measure function in the tools tab within Masslynx, which > > can batch process raw files. > > > DT > > > Matthew Chambers wrote: > >> Hi Bernard, > > >> What is TD and TZ? MassLynx doesn't provide centroiding capability so > >>MassWolfhas a custom centroiding algorithm. I haven't seen any > >> comprehensive comparisons between that custom algorithm and the > >> MaxEnt > >> algorithm available from ProteinLynx Global Server (which is not > >> programmatically accessible even if you have access to PLGS!). > > >> -Matt > > >> Bernt wrote: > > >>> Hi Natalie, > > >>> I'm also currently trying outMassWolf4.2.1......it works with TD > >>> and > >>> TZ as well, although TD appears to be a better option at generating > >>> smaller mzxml files. But the centroiding function doesn't seem to be > >>> working. It gives a "Warning: using EXPERIMENTAL centroiding > >>> functionality" error msg in command prompt and Windows pops up a > >>> "masswolf.exe has encountered a problem and needs to close" error > >>> report. Is the centroiding function still in the process of > >>> development? > > >>> Bernard > > >>> On Jun 25, 5:46 am, Natalie Tasman <[email protected]> > >>> wrote: > > >>>> Hello, > > >>>> First, you might want to check that the file extention is > >>>> indeed .raw > >>>> and not .RAW. Capitalization might make a difference. > > >>>> Can you open this file in the Waters MassLynx application on the > >>>> same > >>>> computer? This must be installed in order for the converter > >>>> (massWolf) to work properly. > > >>>> Natalie > > >>>> On Jun 24, 2009, at 2:39 PM, Hong wrote: > > >>>>> Hi there, > > >>>>> I am new to TPP. I have trouble converting the MassLynx .RAW > >>>>> directory > >>>>> with mzXML. I got the following status report message. What do I > >>>>> need > >>>>> to change to get it work? I greatly appreciate your inputs. > > >>>>> Hong Li > >>>>> NJ Med School > > >>>>>massWolf-v --mzXML c:/Inetpub/wwwroot/ISB/data/hilo08.raw > > >>>>> Settings: > >>>>>centroidscans: 0 > >>>>> compress scans: 0 > >>>>> verbose mode: 1 > >>>>> lockspray: 1 > >>>>> MS^E: 0 > >>>>> threshold: 0 > >>>>> mzML mode: 0 > >>>>> mzXML mode: 1 > >>>>> input filename: c:/Inetpub/wwwroot/ISB/data/hilo08.raw > >>>>> output filename: c:/Inetpub/wwwroot/ISB/data/hilo08.mzXML > >>>>> output file as gzip: 0 > > >>>>> Command FAILED > > > -- > > Dr. David Trudgian > > Bioinformatician in Proteomics > > University of Oxford > > > Tel: (+44) (01865 2)87807 (CCMP - Mon-Thu) > > Tel: (+44) (01865 2)75557 (Dunn Sch - Fri) --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
