Matt, TD filters out data points less than a given intensity, discarding them. TZ sets them to zero. You can produce centroid spectra using the Accurate Mass Measure function in the tools tab within Masslynx, which can batch process raw files.
DT Matthew Chambers wrote: > Hi Bernard, > > What is TD and TZ? MassLynx doesn't provide centroiding capability so > MassWolf has a custom centroiding algorithm. I haven't seen any > comprehensive comparisons between that custom algorithm and the MaxEnt > algorithm available from ProteinLynx Global Server (which is not > programmatically accessible even if you have access to PLGS!). > > -Matt > > > Bernt wrote: > >> Hi Natalie, >> >> I'm also currently trying out MassWolf 4.2.1......it works with TD and >> TZ as well, although TD appears to be a better option at generating >> smaller mzxml files. But the centroiding function doesn't seem to be >> working. It gives a "Warning: using EXPERIMENTAL centroiding >> functionality" error msg in command prompt and Windows pops up a >> "masswolf.exe has encountered a problem and needs to close" error >> report. Is the centroiding function still in the process of >> development? >> >> >> Bernard >> >> >> On Jun 25, 5:46 am, Natalie Tasman <[email protected]> >> wrote: >> >> >>> Hello, >>> >>> First, you might want to check that the file extention is indeed .raw >>> and not .RAW. Capitalization might make a difference. >>> >>> Can you open this file in the Waters MassLynx application on the same >>> computer? This must be installed in order for the converter >>> (massWolf) to work properly. >>> >>> Natalie >>> >>> On Jun 24, 2009, at 2:39 PM, Hong wrote: >>> >>> >>> >>> >>> >>>> Hi there, >>>> >>>> I am new to TPP. I have trouble converting the MassLynx .RAW directory >>>> with mzXML. I got the following status report message. What do I need >>>> to change to get it work? I greatly appreciate your inputs. >>>> >>>> Hong Li >>>> NJ Med School >>>> >>>> massWolf -v --mzXML c:/Inetpub/wwwroot/ISB/data/hilo08.raw >>>> >>>> Settings: >>>> centroid scans: 0 >>>> compress scans: 0 >>>> verbose mode: 1 >>>> lockspray: 1 >>>> MS^E: 0 >>>> threshold: 0 >>>> mzML mode: 0 >>>> mzXML mode: 1 >>>> input filename: c:/Inetpub/wwwroot/ISB/data/hilo08.raw >>>> output filename: c:/Inetpub/wwwroot/ISB/data/hilo08.mzXML >>>> output file as gzip: 0 >>>> >>>> Command FAILED >>>> >>>> >> >> > > > > -- Dr. David Trudgian Bioinformatician in Proteomics University of Oxford Tel: (+44) (01865 2)87807 (CCMP - Mon-Thu) Tel: (+44) (01865 2)75557 (Dunn Sch - Fri) --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
