Do you have MS2 spectrum in the file?  Please see

http://www.mail-archive.com/[email protected]/msg02748.html


On Wed, Jan 5, 2011 at 3:07 PM, Spencer <[email protected]> wrote:

> I have been trying to use the ability to perform an xtandem search in
> TPP 4.4rev1.  I initially had problems with it saying the file is
> corrupt but after changing the extension it seemed to work but now I
> get an error.  The file was exported from an Agilent .d file, but I
> know it works with the standalone install of X! Tandem.
>
> Does anyone have an idea what this error means?  The output is below.
> Thank you.
>
> # Commands for session FHLKKV7M3 on Wed Jan  5 15:46:00 2011
> # BEGIN COMMAND BLOCK
> ###### BEGIN Command Execution ######
> [Wed Jan  5 15:46:00 2011] EXECUTING: run_in c:/Inetpub/wwwroot/ISB/
> data/oetzitest2; c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/
> oetzitest2/otzi_14.tandem.params
> OUTPUT:
>
> X! TANDEM 2009.10.01.1 (LabKey and ISB)
>
> Loading spectra . loaded.
> No input spectra met the acceptance criteria.
>
> command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/
> oetzitest2/otzi_14.tandem.params" failed: Operation not permitted
> END OUTPUT
> RETURN CODE:256
> ###### End Command Execution ######
> # All finished at Wed Jan  5 15:46:00 2011
> # END COMMAND BLOCK
>
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-- 
Joe Slagel
Institute for Systems Biology
[email protected]
(206) 732-1362

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