Ah, that's maybe the point. When I am running msconvert the first time in
cmd, it shows me "ProteoWizard release 2.1.2596" at the end. After that, I
runned msconvert without args and it shows me ProteoWizard relesae
2.1.2132". What should I do?
Cédric

Here are the cmd lines that I got

On 30 March 2011 16:42, Matthew Chambers <[email protected]> wrote:

> I can't reproduce this. Are you sure about the version of msconvert you're
> running? "ProteoWizard release 2.1.2596" shows up when you run msconvert
> without args?
>
> -Matt
>
>
>
> On 3/30/2011 1:42 AM, Cédric Bovet wrote:
>
>> Matt, I tried what you suggested. Unfortunately, it didn't work, msconvert
>> still told me that it
>> "ignores the wrapper" (see below). I am able to read the mzXML, but the
>> file contains both positive
>> and negative ion traces. Here are the command lines and messages from
>> msconvert
>> --------------------------------------------
>> C:\Users\cbovet>msconvert
>> Z:\Toxikologie\LTQVelos\data\Cedric\CBo_1001_110315A\C
>> Bo_1001_110315A_03.raw --32 --mzXML --filter "analyzerType ITMS" --filter
>> "polar
>> ity negative" -o Z:\Toxikologie\MSconvert
>> format: mzXML (Precision_32 [ 1000514:Precision_32 1000515:Precision_32 ],
>> ByteO
>> rder_LittleEndian, Compression_None) indexed="true"
>> outputPath: Z:\Toxikologie\MSconvert
>> extension: .mzXML
>> contactFilename:
>>
>> filters:
>>   analyzerType ITMS
>>   polarity negative
>>
>> filenames:
>>
>> Z:/Toxikologie/LTQVelos/data/Cedric/CBo_1001_110315A/CBo_1001_110315A_03.raw
>>
>> processing file:
>> Z:/Toxikologie/LTQVelos/data/Cedric/CBo_1001_110315A/CBo_1001_1
>> 10315A_03.raw
>> [SpectrumListFactory] Ignoring wrapper: analyzerType ITMS
>> [SpectrumListFactory] Ignoring wrapper: polarity negative
>> writing output file: Z:/Toxikologie/MSconvert/CBo_1001_110315A_03.mzXML
>> ---------------------------------------------
>>
>> Do you have another suggestion? Thanks, Cédric
>>
>>
>> On 29 March 2011 18:58, Matthew Chambers <[email protected]
>> <mailto:[email protected]>> wrote:
>>
>>    Interesting. That appears to be a bug in the config file reader. Until
>> it gets fixed, use the
>>    command-line equivalent for filters, i.e.
>>    --filter "analyzerType ITMS" --filter "polarity negative"
>>
>>    -Matt
>>
>>
>>
>>    On 3/29/2011 11:49 AM, Cédric wrote:
>>
>>        Hi Matt. I used a configuration file (-c) containing the following
>>        information
>>
>>        outdir=Z:\Toxikologie\MSconvert\negmzXMLfiles
>>        mzXML=true
>>        32=true
>>        filter="analyzerType ITMS"
>>        filter="polarity negative"
>>
>>        for the version, it is the one contained with ProteoWizard release
>>        2.1.2596 (ProteoWizard MSdata 2.1.2595, ProteWizardAnalysis
>> 2.1.2587).
>>        Is it the information that you need? Thanks for your help
>>        Cédric
>>
>>        On Mar 29, 5:45 pm, Matthew Chambers<[email protected]
>>        <mailto:[email protected]>>
>>
>>        wrote:
>>
>>            What was your exactly command-line and which version of
>> msconvert did you use?
>>
>>            -Matt
>>
>>            On 3/29/2011 3:07 AM, C dric wrote:
>>
>>                Hi,
>>
>>
>>                I am using msconvert to transform my .raw file acquired on
>> a LTQ Velos
>>                into mzXML file. I performed LC-MS measurements in
>> positive/negative
>>                mode in a single run, therefore my files contain both ion
>> trace (i.e.,
>>                positive and negative). I am trying to extract from my .raw
>> file the
>>                negative ion trace and convert it in mzXML
>> (filter="polarity
>>                negative"). When I am doing that, msconvert ignore my
>> filter (it wrote
>>                back Ignoring wrapper: ) and transform my raw data into
>> mzXML file
>>                which contains the negative and positive ion trace. Does
>> anybody know
>>                how to extract each ion trace separately?
>>
>>
>>                thanks a lot for your help, C dric
>>
>>
>>
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