Hi Chris,

In this URL http://flic.kr/p/axzsk8 there are multiple files, TPP log shows
some permission issue.

Can you try fresh and send the latest log file from "C:\tmp" and see if
there is any thing in Apache logs ?

Please check if simple CGI works...
Create "test.cgi" under "C:\Inetpub\tpp-bin" and add this content, the try
http:/localhost:8080/tpp-bin/test.cgi
#!C:/Perl/bin/perl
print "Content-type:text/html\n\n";
print "OK";

Also, looks like there are multiple installation of perl as per your path...


On Fri, Oct 21, 2011 at 1:23 PM, Chris L <[email protected]> wrote:

> Hi Vishal and all,
> I am doing step 7: "Visualize LC-MS/MS data using Pep3D " in TPP Demo
> 2009 (http://tools.proteomecenter.org/wiki/index.php?
> title=TPP_Demo2009)
> a) I had C:\tmp to begin with
> b) I am using firefox. I tried to open it with IE6 and encountered the
> same problem.
> c) I did not have user variable WEBSERVER_ROOT and had to create one
> I had PATH's value as
> C:\Inetpub\tpp-bin;C:\Program Files\Perl\site\bin;C:\Program Files\Perl
> \bin;C:\Xcalibur\system\programs;C:\Program Files\Thermo\Foundation;C:
> \cygwin\usr\X11R6\bin;C:\cygwin\bin;C:\Perl\site\bin;C:\Perl\bin;J:
> \oracle\ora92\bin;C:\Program Files\Oracle\jre\1.1.8\bin;C:\Perl\bin;
> %SystemRoot%\system32;%SystemRoot%;%SystemRoot%\System32\Wbem;C:
> \Program Files\Intel\DMIX;C:\cygwin\usr\X11R6\bin;C:\cygwin\bin;C:
> \Program Files\QuickTime\QTSystem\;C:\strawberry\c\bin;C:\strawberry
> \perl\site\bin;C:\strawberry\perl\bin
> so I changed it to yours, and rebooted the computer, which still did
> not solve the problem.
> d) Heres my snippet of "httpd.conf" in Apache
> #
> # Begin settings for the Trans Proteomic Pipeline
> #
> Alias /tpp-bin "C:/Inetpub/tpp-bin"
> <Directory "C:/Inetpub/tpp-bin">
>    Options Indexes MultiViews ExecCGI
>    AllowOverride None
>    Order allow,deny
>    Allow from all
>
>    AddHandler cgi-script .cgi .pl
>    ScriptInterpreterSource Registry
>
>    PassEnv WEBSERVER_ROOT
>     PassEnv WEBSERVER_TMP
> </Directory>
>
> Alias /ISB "C:/Inetpub/wwwroot/ISB"
>
> Alias /schema "C:/Inetpub/wwwroot/schema"
>
> <Directory "C:/Inetpub/wwwroot">
>    Options Indexes MultiViews Includes
>     AllowOverride None
>    Order allow,deny
>    Allow from all
>
>    AddType text/html .shtml
>    AddHandler server-parsed .shtml
>
>    PassEnv WEBSERVER_ROOT
>     PassEnv WEBSERVER_TMP
> </Directory>
> #
> # End settings for the Trans Proteomic Pipeline
> #
>
> Thanks in advance for your advices.
>
> Regards,
> Chris
>
> On Oct 20, 5:39 pm, Vishal Swaroop <[email protected]> wrote:
> > Hi Chris,
> >
> > Good to know that it worked..
> >
> > I am not sure about step7 (please send me the url you are referring).
> >
> > I am sure there were lot of things dependent on OS.. may be try this...
> > restart after if changing variables as I think System variable change
> > requires restart but not user variable change... so just re-start.. :)
> >
> > a) I assume you have default data directory i.e. "C:" Check if you have
> > "C:\tmp", if not then create it.
> > b) Which browser you are using, I am using IE8 and it works fine.
> > c) Right click : "My Computer">Properties>Advanced Tab> Environment
> > variables
> > Check User variable :
> > Variable name : WEBSERVER_ROOT
> > Variable value : C:\Inetpub\wwwroot
> >
> > Check System Variables :
> > Variable name "PATH" should have
> > C:\Inetpub\tpp-bin;C:\Perl\site\bin;C:\Perl\bin;
> >
> > d) Here is snippet of Apache "httpd.conf" file which you can compare..
> >
> > #
> > # Begin settings for the Trans Proteomic Pipeline
> > #
> >
> > # Add 5-hour timeout
> > Timeout 18000
> >
> > AddType text/html .shtml
> > AddHandler server-parsed .shtml
> >
> > SetEnv WEBSERVER_ROOT C:/Inetpub/wwwroot
> > SetEnv WEBSERVER_URLhttp://localhost:81
> >
> > Alias /tpp-bin "C:/Inetpub/tpp-bin"
> > <Directory "C:/Inetpub/tpp-bin">
> >     Options Indexes MultiViews ExecCGI
> >     AllowOverride None
> >     Order allow,deny
> >     Allow from all
> >
> >     AddHandler cgi-script .cgi .pl
> >     ScriptInterpreterSource Registry
> >     PassEnv WEBSERVER_ROOT
> > </Directory>
> >
> > Alias /ISB "C:/Inetpub/wwwroot/ISB"
> > Alias /isb "C:/Inetpub/wwwroot/ISB"
> > <Directory "C:/Inetpub/wwwroot/ISB">
> >     Options Indexes MultiViews Includes ExecCGI
> >     AllowOverride None
> >     Order allow,deny
> >     Allow from all
> >
> >     AddType text/html .shtml
> >     AddHandler server-parsed .shtml
> >     PassEnv WEBSERVER_ROOT
> > </Directory>
> >
> >
> >
> >
> >
> >
> >
> > On Thu, Oct 20, 2011 at 7:02 PM, Chris L <[email protected]> wrote:
> > > Hi all,
> > > I am still encountering problem with tutorial step 6 which I am
> > > ignoring for now.
> > > In step 7, I am not able to open pepXML viewer for my interact.pep.xml
> > > and here's the screen I get when trying to open it:
> > >http://flic.kr/p/axzsk8
> > > Any tip appreciated. Thanks again.
> >
> > > Regards,
> > > Chris
> >
> > > On Oct 20, 3:39 pm, Chris L <[email protected]> wrote:
> > > > Changing the domain to localhost:8080 solved the problem.
> > > > Thanks a lot Vishal.
> >
> > > > Regards,
> > > > Chris
> >
> > > > On Oct 19, 6:55 am, Vishal Swaroop <[email protected]> wrote:
> >
> > > > > Which browser you are using, firefox ?, try using IE or other
> >
> > > > > First check if Apache is configured correctly, tryhttp://localhost,
> you
> > > > > should see "It works!" message on browser, if you have port other
> than
> > > > > default (80) then usehttp://localhost:<your port>
> >
> > > > > Then try http://<localhost>:<port>/tpp-bin/tpp_gui.pl, if you have
> not
> > > > > changed the port then it should be
> > >http://localhost/tpp-bin/tpp_gui.pl
> >
> > > > > ..\Apache...\conf\httpd.conf should have information of host and
> port.
> >
> > > > > Hope this helps..
> >
> > > > > On Tue, Oct 18, 2011 at 7:46 PM, Chris L <[email protected]> wrote:
> > > > > > localhost/tpp-bin/tpp_gui.pl
> >
> > > > > > The web browser changes it towww.localhost.comautomatically.
> >
> > > > > > Chris
> >
> > > > > > On Oct 18, 4:44 pm, Chris L <[email protected]> wrote:
> > > > > > > No, I havehttp://www.localhost.com/tpp-bin/tpp_gui.pl
> > > > > > > Formatting of my post caused the confusion
> >
> > > > > > > Regards,
> > > > > > > Chris
> >
> > > > > > > On Oct 18, 4:19 pm, "Vensel, William" <
> [email protected]
> >
> > > > > > > wrote:
> >
> > > > > > > > Hi Chris,
> >
> > > > > > > > Is there a blank space in yur web address? // Local vs
> //local
> >
> > > > > > > > Bill
> >
> > > > > > > > -----Original Message-----
> > > > > > > > From: [email protected] [mailto:
> > > > > > [email protected]] On Behalf Of Chris L
> > > > > > > > Sent: Tuesday, October 18, 2011 3:28 PM
> > > > > > > > To: spctools-discuss
> > > > > > > > Subject: [spctools-discuss] Re: TPP Demo 2009
> >
> > > > > > > > Hi Luis and all,
> >
> > > > > > > > I have uninstalled Microsoft IIS from the windows component
> > > (running
> > > > > > > > windows XP) and re-installed TPP v4.5 with the default Apache
> > > > > > > > webserver. Now the problem is when I try to access the GUI
> > > (http://
> > > > > > > > localhost/tpp-bin/tpp_gui.pl), I get a blank page.
> Refreshing
> > > the page
> > > > > > > > and rebooting doesn't help. Any tips and advice appreciated.
> >
> > > > > > > > Sorry for all the miniscule questions and thanks again for
> being
> > > > > > > > patient.
> >
> > > > > > > > Regards,
> > > > > > > > Chris
> >
> > > > > > > > On Oct 14, 4:11 pm, Luis Mendoza <
> > > [email protected]>
> > > > > > > > wrote:
> > > > > > > > > Hello Chris,
> > > > > > > > > From the "timeout" error screenshot, it seems that you are
> > > using the
> > > > > > IIS
> > > > > > > > > server.  The TPP does not support this web server; instead
> we
> > > > > > recommend that
> > > > > > > > > you install and run the Apache web server -- which is
> bundled
> > > and
> > > > > > installed
> > > > > > > > > with the TPP (provided you check the default boxes in the
> > > install
> > > > > > wizard).
> >
> > > > > > > > > Hope this helps,
> > > > > > > > > --Luis
> >
> > > > > > > > > On Fri, Oct 14, 2011 at 4:04 PM, Chris L <[email protected]>
> > > wrote:
> > > > > > > > > > Dear all,
> >
> > > > > > > > > > I am experimenting with the TPP demo 2009 (http://
> > > > > > > > > >
> tools.proteomecenter.org/wiki/index.php?title=TPP_Demo2009)
> > > and
> > > > > > > > > > encountered two problems.
> >
> > > > > > > > > > First, for step 6: Validation of Peptide-Spectrum
> assignments
> > > with
> > > > > > > > > > PeptideProphet,  I can never get past the Run XInteract
> step.
> > > My
> > > > > > > > > > command does not finish running even after over a day!
> > > (screen:
> > > > > > > > > >http://flic.kr/p/avNB4R)
> >
> > > > > > > > > > Second, when I am trying step 7: Visualize LC-MS/MS data
> > > using
> > > > > > Pep3D.
> > > > > > > > > > My browser tab shows "Connecting..." and a blank page
> that
> > > does not
> > > > > > > > > > load and eventually turns into a CGI error page (screen:
> > > > > > > > > >http://flic.kr/p/avNAXk)
> >
> > > > > > > > > > Any suggestion appreciated. Thank you.
> >
> > > > > > > > > > Regards,
> > > > > > > > > > Chris
> >
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