Well, I could be wrong, but that space in the directory name is probably
the culprit.  I would rename the directory .../data/X! Tandem to something
without a space in it.  Just call it .../data/tandem instead.

Cheers,

Brian

Brian Hampton
Protein Analysis Lab
Center for Vascular and Inflammatory Diseases
University of Maryland School of Medicine
800 West Baltimore Street Rm 307
Baltimore  MD  21201
V: 410-706-8207
F: 410-706-8234


On Wed, Jul 17, 2013 at 5:24 PM, Kierstin Schmidt <
[email protected]> wrote:

> Hi!
> I am a new user of TPP.  I am trying to load my mzXML files to be searched
> by X! Tandem, and I keep getting the following errors.  If anyone has
> advice, it would be greatly appreciated!
>
> Thanks!
>
> Kierstin
>
>
>  "c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem.pep.xml (*file
> not found:* not created or deleted?)
>
> Followed by
>
>  run_in c:/Inetpub/wwwroot/ISB/data/X!Tandem; c:\Inetpub\tpp-bin\tandem 
> c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem.params
>
> ------------------------------
>
> X! TANDEM 2010.10.01.1 (LabKey, Insilicos and ISB)
>
> Loading spectra .
> Failed to open: "c:/Inetpub/wwwroot/ISB/data/X!Tandem"
> The input parameter file "c:/Inetpub/wwwroot/ISB/data/X!Tandem" could not be 
> located.
> Check the file path name and try again.
>
>
> An error was detected while loading the input parameters.
> Please follow the advice above or contact a GPM administrator to help you.
> command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/X! 
> Tandem/MS138QE_sample2.tandem.params" failed: Unknown error
>
> *Command FAILED*
>
>
>   Command 2 *[Wed Jul 17 17:11:38 2013] *       [ Show / Hide ]
>
>  run_in c:/Inetpub/wwwroot/ISB/data/X!Tandem; c:\Inetpub\tpp-bin\Tandem2XML 
> c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem 
> c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem.pep.xml
>
> ------------------------------
>
>  c:\Inetpub\tpp-bin\Tandem2XML(TPP v4.5 RAPTURE rev 2, Build 201202031108 
> (MinGW))
> Usage: c:\Inetpub\tpp-bin\Tandem2XML <input-file> [<output-file>]
>
> command "c:\Inetpub\tpp-bin\Tandem2XML c:/Inetpub/wwwroot/ISB/data/X! 
> Tandem/MS138QE_sample2.tandem c:/Inetpub/wwwroot/ISB/data/X! 
> Tandem/MS138QE_sample2.tandem.pep.xml" failed: Operation not permitted
>
> *Command FAILED*
>
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