Along these lines, everyone should simply avoid any symbols in directory
and file names.  There are some character symbols that are OK (I tell folks
to use dash '-' and underscore '_' only besides letters and numbers) but
it's best to avoid them all if you don't know which ones will work.


On Wed, Jul 17, 2013 at 3:28 PM, Brian Hampton <[email protected]> wrote:

> Well, I could be wrong, but that space in the directory name is probably
> the culprit.  I would rename the directory .../data/X! Tandem to something
> without a space in it.  Just call it .../data/tandem instead.
>
> Cheers,
>
> Brian
>
> Brian Hampton
> Protein Analysis Lab
> Center for Vascular and Inflammatory Diseases
> University of Maryland School of Medicine
> 800 West Baltimore Street Rm 307
> Baltimore  MD  21201
> V: 410-706-8207
> F: 410-706-8234
>
>
> On Wed, Jul 17, 2013 at 5:24 PM, Kierstin Schmidt <
> [email protected]> wrote:
>
>> Hi!
>> I am a new user of TPP.  I am trying to load my mzXML files to be
>> searched by X! Tandem, and I keep getting the following errors.  If anyone
>> has advice, it would be greatly appreciated!
>>
>> Thanks!
>>
>> Kierstin
>>
>>
>>  "c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem.pep.xml (*file
>> not found:* not created or deleted?)
>>
>> Followed by
>>
>>  run_in c:/Inetpub/wwwroot/ISB/data/X!Tandem; c:\Inetpub\tpp-bin\tandem 
>> c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem.params
>>
>> ------------------------------
>>
>> X! TANDEM 2010.10.01.1 (LabKey, Insilicos and ISB)
>>
>> Loading spectra .
>> Failed to open: "c:/Inetpub/wwwroot/ISB/data/X!Tandem"
>> The input parameter file "c:/Inetpub/wwwroot/ISB/data/X!Tandem" could not be 
>> located.
>> Check the file path name and try again.
>>
>>
>> An error was detected while loading the input parameters.
>> Please follow the advice above or contact a GPM administrator to help you.
>> command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/X! 
>> Tandem/MS138QE_sample2.tandem.params" failed: Unknown error
>>
>> *Command FAILED*
>>
>>
>>   Command 2 *[Wed Jul 17 17:11:38 2013] *       [ Show / Hide ]
>>
>>  run_in c:/Inetpub/wwwroot/ISB/data/X!Tandem; c:\Inetpub\tpp-bin\Tandem2XML 
>> c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem 
>> c:/Inetpub/wwwroot/ISB/data/X! Tandem/MS138QE_sample2.tandem.pep.xml
>>
>> ------------------------------
>>
>>  c:\Inetpub\tpp-bin\Tandem2XML(TPP v4.5 RAPTURE rev 2, Build 201202031108 
>> (MinGW))
>> Usage: c:\Inetpub\tpp-bin\Tandem2XML <input-file> [<output-file>]
>>
>> command "c:\Inetpub\tpp-bin\Tandem2XML c:/Inetpub/wwwroot/ISB/data/X! 
>> Tandem/MS138QE_sample2.tandem c:/Inetpub/wwwroot/ISB/data/X! 
>> Tandem/MS138QE_sample2.tandem.pep.xml" failed: Operation not permitted
>>
>> *Command FAILED*
>>
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