I encounter the same problem. Tandem still not works after centroiding all scans and compressing peaklists.
在 2011年10月18日星期二UTC+8下午1时36分47秒，furukie写道： > > Hi Luis, > > the inputs are for standard SILAC search which worked fine for most > files.. The error I get in the log were; > > [Thu Oct 13 16:52:38 2011] EXECUTING: run_in c:/Inetpub/wwwroot/ISB/ > data/P00525; c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/ > P00525/Mix14-3-3PHHUP00525G00416GL01B015.tandem.params > OUTPUT: > > X! TANDEM 2009.10.01.1 (LabKey and ISB) > > Loading spectra . > This application has requested the Runtime to terminate it in an > unusual way. > Please contact the application's support team for more information. > > command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/P00525/ > Mix14-3-3PHHUP00525G00416GL01B015.tandem.params" failed: No such > process > END OUTPUT > RETURN CODE:768 > ###### End Command Execution ###### > ###### BEGIN Command Execution ###### > [Thu Oct 13 17:00:08 2011] EXECUTING: run_in c:/Inetpub/wwwroot/ISB/ > data/P00525; c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/ > P00525/Mix14-3-3PHHUP00525G00416GL01B016.tandem.params > OUTPUT: > > X! TANDEM 2009.10.01.1 (LabKey and ISB) > > Loading spectra . > This application has requested the Runtime to terminate it in an > unusual way. > Please contact the application's support team for more information. > > command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/P00525/ > Mix14-3-3PHHUP00525G00416GL01B016.tandem.params" failed: No such > process > END OUTPUT > RETURN CODE:768 > > The thing is, out of my 24 sample, 5 of them will get this error. I > looked at the spectra, they are fine. The files were not corrupted. > The only thing would be they have very high spectra like MS/MS at 7xe7 > kind of range and that the .d files are nearly 2gb.. Could it be my > tandem input parameters are just not suitable for that kind of range? > I tried increasing the dynamic range/total peaks, etc, but it doesn't > work.. I need those files to complete my set of data. > > -Eiko > > On Oct 17, 1:07 pm, Luis Mendoza <[email protected]> > wrote: > > Hi Eiko, > > The tandem parameters files are required to launch the search, and they > need > > to have the ".xml" extension for Petunia to read them. The files you > seem > > to be referring to are the tandem output files (which you will then > convert > > to pepXML) which are the output of the search. What do you mean by > > "terminated in an unusual way"? What are the inputs that you are giving > > tandem, and what does it say in the error log? > > --Luis > > > > On Sun, Oct 16, 2011 at 8:47 PM, furukie <[email protected]> > wrote: > > > Hi Luis, > > > > > I've checked and they are .xml. However, I am having problems to even > > > reach that "stage" in TPP for some files. Like I've previously > > > mentioned, my database search gets terminated in an unusual way and I > > > was told to contact the administrator. However, for most files, it > > > runs fine... > > > > > Thanks, > > > > > Eiko > > > > > On Oct 14, 4:09 am, Luis Mendoza <[email protected]> > > > wrote: > > > > Hello Eiko, > > > > Petunia (the TPP GUI) can only 'detect' tandem parameters files that > end > > > > with the ".xml" extension (e.g. "tandem.params.xml"). Please try > > > renaming > > > > them in such a way and see if your searches and conversions work. > > > > Cheers, > > > > --Luis > > > > > > On Thu, Oct 13, 2011 at 12:23 AM, furukie <[email protected]> > > > > wrote: > > > > > Hi, > > > > > > > From file logs, I notice that the files could not be read because > the > > > > > search was terminated in an unusual way. And ask to contact the > > > > > administrator. This error pops up selectively. At first, I thought > it > > > > > was the computer specs being too weak and got a new computer with > much > > > > > stronger specs. However, this problem still persists. Any ideas? > I'm > > > > > desperately in need of those files for data analysis. It seem to > run > > > > > well for small raw files but not the larger ones. > > > > > > > Many thanks. > > > > > > > -Eiko > > > > > > > -- > > > > > You received this message because you are subscribed to the Google > > > Groups > > > > > "spctools-discuss" group. > > > > > To post to this group, send email to > > > > > [email protected]<javascript:> > > > . > > > > > To unsubscribe from this group, send email to > > > > > [email protected] <javascript:>. > > > > > For more options, visit this group at > > > > >http://groups.google.com/group/spctools-discuss?hl=en. > > > > > -- > > > You received this message because you are subscribed to the Google > Groups > > > "spctools-discuss" group. > > > To post to this group, send email to > > > [email protected]<javascript:>. > > > > To unsubscribe from this group, send email to > > > [email protected] <javascript:>. > > > For more options, visit this group at > > >http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. 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