My guess is it could be related to the spaces in you folder names.  Can you
try folder names without spaces?

Thanks,
-David

On Fri, Mar 20, 2015 at 9:35 AM, David Baxter <[email protected]> wrote:

> Hello,
>
> I am trying to use TPP for the first time and have been unable to convert
> .dat files from mascot into pepXML.  I suspect that maybe I am not using
> the right database file.
>
> I obtained the .dat files from the free mascot server and did my searches
> using the NCBInr database restricted to zebrafish.  I also downloaded the
> nr database from ftp://ftp.ncbi.nih.gov/blast/db/FASTA/nr.gz and unziped
> that file to use for Mascot2XML.
>
> The error message I receive when trying to convert is pasted below.  If
> anyone can tell me what I am doing wrong, I would appreciate it very much.
>
> Thanks
>
>
>
> *EXECUTING: cd c:/Inetpub/wwwroot/ISB/data/Baxter MS Data&
> c:\Inetpub\tpp-bin\Mascot2XML c:/Inetpub/wwwroot/ISB/data/Baxter MS
> Data/FTgTSnEEE.dat -Dc:/Inetpub/wwwroot/ISB/data/Baxter MS Data/nr
> -Etrypsin -notgz *
>
> This application has requested the Runtime to terminate it in an unusual way.
> Please contact the application's support team for more information.
>
> *Command FAILED*
> RETURN CODE:65280
>
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