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It could also happen if your geometry is very relaxed. Have a look
rmsd for bond lengths. If they are high then you may consider
using tighter restraints. In case of refmac you may need to use
smaller matrix value for weighting.
Garib
On 5 Jul 2006, at 13:24, Li Sheng wrote:
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Hi, dear all,
Please do me a favour. I ever collected a data set of 2.7A. The
space group is C2. The model fit the 2fofc map and the omit map
very well. The R factor is 0.26 now but R free is 0.37 and can not
be minished. What kind of error can cause such a gap between R
factor and R free?
Thanks in advance.
Sincerely yours,
Li
2006.06.07