Am 24.02.2015 um 10:03 schrieb Peter Cock:
> On Tue, Feb 24, 2015 at 6:31 AM, Michael Thon <mike.t...@gmail.com> wrote:
>> The main problem for me was that I was not aware that the tophat
>> tool had a dependency on a command line tool outside of galaxy
>> installation.  I see in the admin interface that tophat has a dependency
>> on samtools of type package and I assumed that should provide the
>> samtools binary.  Of course I have not read the manuals regarding
>> tool sheds, dependencies, etc so thats probably where my problem
>> started in the first place...
> 
> I can see your chain of thought here. Unfortunately while the tophat
> tool (or any tool) may explicitly use samtools (and request a particular
> version via the dependencies system), once the tool has finished it
> has produced a BAM file. So far so good, but at that point Galaxy
> tries to index the BAM file itself. This happens for any BAM file,
> regardless of the tool which created it - and simply expects samtools
> on the $PATH.
> 
> If this is on the wiki (core dependencies of Galaxy itself), could
> someone post the URL here please?

It is not listed here:
https://wiki.galaxyproject.org/Admin/Config/ToolDependenciesList

I can be wrong but I think Galaxy will search in
<galaxy-tools>/samtools/default if samtools is not on your $PATH.

Can anyone confirm this?

Ciao,
Bjoern

> Peter
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