Re: [galaxy-dev] GATK 3.1

2014-03-18 Thread Björn Grüning
Hi Thomas, we are have some rough plans to do that, as Nicolas already mentioned. But at first we want to release one final GATK2.8 wrapper. Help is very much appreciated. Cheers, Bjoern Am 18.03.2014 14:41, schrieb Berner, Thomas: Hi, I've seen there is a new version (3.1-1) of GATK availa

Re: [galaxy-dev] GATK 3.1

2014-03-18 Thread Nicola Soranzo
Il 2014-03-18 14:41 Berner, Thomas ha scritto: Hi, I've seen there is a new version (3.1-1) of GATK available at http://www.broadinstitute.org/gatk/download [1] . Are there any plans of getting this version into Galaxy in the nearer future? Hi Thomas, as you probably know there is a wrapper f

Re: [galaxy-dev] GATK

2013-07-29 Thread Sridhar Srinivasan
Thanks for the suggestin Linda... i removed the line -et "NO_ET" ##ET no phone home Now i am not getting the Error as get key to run the GATK.. But i am getting error as the fasta file is not found.. even in backend i found the command as mentioned below in the reference file section "-R " the

Re: [galaxy-dev] GATK tools not showing up in cloudman Galaxy installation

2013-06-24 Thread Marco Ocana
Hi Dan, Thanks for the help. The Galaxy missing tools are now loaded and working as expected. Best regards, Marco On Thu, Jun 20, 2013 at 3:36 PM, Daniel Blankenberg wrote: > Hi Marco, > > You'll need to enable some missing tool data tables in > tool_data_table_conf.xml. i.e. adding: > >

Re: [galaxy-dev] GATK tools not showing up in cloudman Galaxy installation

2013-06-20 Thread Daniel Blankenberg
Hi Marco, You'll need to enable some missing tool data tables in tool_data_table_conf.xml. i.e. adding: value, dbkey, name, path value, name, gatk_value, tools_valid_for Also make sure the files exist at the listed , they probably

Re: [galaxy-dev] GATK

2013-06-11 Thread sridhar srinivasan
Hello Linda, Thanks for your suggestions. i removed the line from xml file but now i am getting different error. # ERROR -- # ERROR A USER ERROR has occurred (version 2.5-2-gf57256b): # ERROR The

Re: [galaxy-dev] GATK

2013-06-06 Thread Linda Cham
Hi Sridhar, About the error, you need to request a key from GATK http://gatkforums.broadinstitute.org/discussion/1250/what-is-phone-home-and-how-does-it-affect-me#latest See under "How to obtain and use a GATK key" Or by pass it by removing the -et "NO_ET" line from the xml files. From:

Re: [galaxy-dev] gatk lite

2013-01-03 Thread Anthonius deBoer
You could rename the file from "GenomeAnalysisTKLite.jar" to "GenomeAnalysisTK.jar" in the directory where you placed GATK...Something like/home/svcgalaxy/g/tool-data/shared/jars/gatkand it should work...ThonOn Jan 03, 2013, at 02:54 PM, mark.r...@syngenta.com wrote:Hello Is it possible to redirect

Re: [galaxy-dev] GATK v2.x

2012-09-11 Thread James Taylor
David, because of the restrictive licensing terms of GATK2 the main Galaxy Team will not continue to develop any GATK tools except for those which remain under the open source license (basically, GATK 1.6). We will be very happy to have wrappers for these and other non-open-source tools contribute

Re: [galaxy-dev] GATK tools

2012-04-12 Thread Daniel Blankenberg
Hi Rob, At this time, these tools are still included within the main Galaxy distribution under tools/gatk. I would recommend using the latest version available from galaxy-central until they are migrated into the toolshed. Please let us know if you encounter any issues. Thanks for using Gala

Re: [galaxy-dev] GATK Depth of Coverage error

2012-03-02 Thread Daniel Blankenberg
Hi Curt, This should be fixed in -central (https://bitbucket.org/galaxy/galaxy-central) in changeset 6778:35be930b21be and will be available in the next dist release. If you don't want to wait until the next dist release, you can also pull directly from central or apply the changes manually.

Re: [galaxy-dev] GATK metadata problems

2012-02-27 Thread Daniel Blankenberg
Hi Curt, This is an annoyance when trying to use these tools interactively, but running or designing a workflow in the editor should work just fine. The issue is that for Galaxy the BAM index is technically considered an optional metadata attribute, but it is required for GATK. I've removed the

Re: [galaxy-dev] GATK / R local install configuration

2011-12-05 Thread Daniel Blankenberg
Hi Carlos, Are you using a version of GATK that is 1.3? Another possibility is that you are missing an R library (e.g. 'ggplot2'), that is used by the VariantRecalibrator for building the plots. Start up an interactive R session and type: "library('ggplot2')" If you are missing the library, t

Re: [galaxy-dev] GATK / R local install configuration

2011-12-01 Thread Carlos Borroto
Hi, Any chances someone could give me hint on this issue? Thanks in advance, Carlos On Tue, Nov 29, 2011 at 2:02 PM, Carlos Borroto wrote: > Hi, > > I'm testing the GATK pipeline and I ran into a problem with "Variant > Recalibrator" tool. It seems I don't have correctly configure R and > GATK

Re: [galaxy-dev] GATK unified genotyper

2011-09-29 Thread Daniel Blankenberg
Hi Ilya, Something similar to this is planned, but directly referencing location files is being deprecated in favor of using the tool_data_tables mechanism. The validator implementation does not yet support data tables, but will be enhanced in the future. Additionally, the selection functional

Re: [galaxy-dev] GATK unified genotyper

2011-09-28 Thread Chorny, Ilya
To prevent user error in mixing genomes, would you be interested in a feature in which the unified genotyper choses the genome in a fashion similar to how samtools know which which genome to use? Ilya Chorny Ph.D. Bioinformatics Scientist I Illum

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-16 Thread Daniel Blankenberg
Hi Ilya, --genotype_likelihoods_model / -glm is available under advanced options for the tool; it can be set to both, snp or indel. This parameter might be pulled out from the advanced options heading and placed on the base options in the future to make it easier to access. Thanks for the sugg

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-09 Thread Chorny, Ilya
The newest version of the Unified Genotyper has a -glm option which needs to be set to both to call indels. BTW, any interest in writing a wrapper for the Depth of Coverage tool? Thanks, Ilya From: Daniel Blankenberg [mailto:d...@bx.psu.edu] Sent: Wednesday, September 07, 2011 10:57 AM To: Ch

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-07 Thread Daniel Blankenberg
Hi Ilya, The JVM can be quite a pain when determining the best way to determine max memory, however the underlying gatk_wrapper.py script was updated last week in 5970:20215fcf6da7 to allow using -Xmx or XX:DefaultMaxRAMFraction. Each of the GATK tools are currently already set to use 'XX:Defau

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-07 Thread Chorny, Ilya
Hi Daniel, I have been implementing the GATK unified genotyper and I was having some issues. Occasionally I would get an error that the Java virtual machine would not start. I got around that by adding the Xmx3g parameter to the command line. I also added the -nt 3 because otherwise it's really