Re: [galaxy-user] Galaxy User List Being Retired on June 6, 2014.

2014-06-02 Thread Daniel Blankenberg
I think this is great. Do we have it clearly explained on the wiki somewhere about how to turn on mailing list mode within biostar? I didn’t see anything in a quick search, but it would probably help ease the transition for people that just love the mailing list approach. I also find it

Re: [galaxy-user] Base Recalibration tool on usegalaxy

2014-04-15 Thread Daniel Blankenberg
. Regards, Kaz 2014-04-12 13:08 GMT+09:00 Daniel Blankenberg d...@bx.psu.edu: Hi Kaz, We do have a problem with the Table Recalibration tool on Main right now. We'll let you know when we have a fix. Thanks for using Galaxy, Dan On Apr 11, 2014, at 10:24 PM, KS k...@kyudai.jp wrote

Re: [galaxy-user] Base Recalibration tool on usegalaxy

2014-04-11 Thread Daniel Blankenberg
Hi Kaz, We do have a problem with the Table Recalibration tool on Main right now. We’ll let you know when we have a fix. Thanks for using Galaxy, Dan On Apr 11, 2014, at 10:24 PM, KS k...@kyudai.jp wrote: Hi Bjoern, Thank you for the reply. I found Table Recalibration, probably from

Re: [galaxy-user] Can not delete data from my local galaxy

2014-04-10 Thread Daniel Blankenberg
Hi Nishant, In your universe_wsgi.ini file, you need to set: allow_user_dataset_purge = True Thanks for using Galaxy, Dan On Apr 10, 2014, at 8:09 AM, Nishant THAKUR tha...@ciml.univ-mrs.fr wrote: Hello, I am using local galaxy server. I have disc storage problem and I noticed that

Re: [galaxy-user] How do I remove Histories shared with you by others?

2014-02-06 Thread Daniel Blankenberg
Hi Casey, I was able to confirm this issue and we’ll work on a fix for it. Thanks for reporting. Thanks for using Galaxy, Dan On Feb 6, 2014, at 10:25 AM, Jennifer Jackson j...@bx.psu.edu wrote: Hi Casey, I just tested the UI unsharing for both of your cases and was unable to

Re: [galaxy-user] [galaxy-dev] Connecting to new Galaxy Cloudman by FTP

2013-07-16 Thread Daniel Blankenberg
Hey Mo, You can use ssh to connect to the Galaxy machine. If you used cloudlaunch to create your instance, it should display an example connection string that will work from e.g. a linux/mac shell, something like: 'ssh -i cloudman_keypair.pem ubuntu@IP', after your instance launched. Once

Re: [galaxy-user] Unable to use FASTQ tool on Main over several days

2013-05-24 Thread Daniel Blankenberg
Hi Eduardo, I've committed some code that should fix the issue that you are experiencing, and it will be available on the main server after the next update (some time next week). Until then, if you change the name of e.g. your history item 34 to not have a 'í' or other non-ascii characters

Re: [galaxy-user] problem uploading txt data

2013-05-14 Thread Daniel Blankenberg
Hi Chunyu, Thanks for reporting this issue, it has been resolved in our stable branch and will be available on our public server after the next update. Thanks for using Galaxy, Dan On May 12, 2013, at 10:52 AM, Chunyu Liu wrote: hi, I had an odd problem today, seems not a problem

Re: [galaxy-user] Change format with edit attributes

2013-04-03 Thread Daniel Blankenberg
Hi Gema, For example, see the Combine FASTA and QUAL into FASTQ tool for creating a FASTQ file from FASTA data; when quality data is not provided, fake score values will be used. Thanks for using Galaxy, Dan On Apr 3, 2013, at 10:42 AM, Peter Cock wrote: On Wed, Apr 3, 2013 at 3:40

Re: [galaxy-user] Discrepancy between Intersect and Join

2013-02-08 Thread Daniel Blankenberg
Hi Aaron, I just tested this small example and it reported one region as the result of the intersect: https://main.g2.bx.psu.edu/u/dan/h/aaron-quinlan-intersect-test-02-08-2013 Do you have a history available that you can share (privately if you desire) where you see the issue, and we'll take

Re: [galaxy-user] Discrepancy between Intersect and Join

2013-02-08 Thread Daniel Blankenberg
zero intersections. Join, in contrast, works fine. I'd be happy to share the files. Would it be best to send them directly to you by email? The are small. Thanks much for the help, - Aaron quinlanlab.org On Feb 8, 2013, at 2:56 PM, Daniel Blankenberg d...@bx.psu.edu wrote

Re: [galaxy-user] How to display data at UCSC main?

2012-12-21 Thread Daniel Blankenberg
Hi Anna, Thank you for reporting this error. There is currently an issue with displaying the link in the history item for the way we normally display interval datasets at UCSC. However, we have an alternative method available and I have enabled that for now. You will need to refresh the

Re: [galaxy-user] GATK Not running

2012-12-12 Thread Daniel Blankenberg
Hi Umar, Can you click the eye icon to view the contents of the 'log' dataset for the GATK run. The end of the log should have the actual error encountered (the text you provided is a bit of a red herring) Since you are using hg19, the most likely cause for the error is that the reference

Re: [galaxy-user] GATK Not running

2012-12-12 Thread Daniel Blankenberg
Hi Philippe, The GATK wrappers provided with the Galaxy distribution are for GATK version 1.4. There is a set of 1.6/GATK-lite wrappers that has been developed by the team, but is not yet available. There may also be other options available in the Tool Shed that have been contributed by the

Re: [galaxy-user] GATK Not running

2012-12-12 Thread Daniel Blankenberg
Hi Joshua, Is this on the main public site? If so, can you share your history with me and I'll take a look? If this is on a local instance, can you provide additional information, such as the GATK version that you are using? Thanks for using Galaxy, Dan On Dec 11, 2012, at 5:34 PM, Joshua

Re: [galaxy-user] Warning message

2012-11-13 Thread Daniel Blankenberg
Hi Rolando, You'll need to remove the old blast datatypes from your datatypes_conf.xml file. If you haven't added any new datatypes manually yourself, you can copy the datatypes_conf.xml.sample file over of your datatypes_conf.xml file. Also, in the future questions about local Galaxy

Re: [galaxy-user] Error message on local install

2012-10-11 Thread Daniel Blankenberg
Hi Chris, Are you able to share a copy of the offending file? Perhaps by uploading to the public Galaxy server, possibly trying the Grooming step there, and then sharing the history with me or by using the bug report option on the Grooming step if it fails? If not, you could look at the

Re: [galaxy-user] job information on galaxy

2012-10-11 Thread Daniel Blankenberg
Hello, Thanks for reporting this error. This has been fixed in changesets 7875:62b89df24ab1 through 7879:046634d05007 in galaxy-central and will be included in the next distribution and main update. Please let us know if you encounter additional issues after the update. Thanks for using

Re: [galaxy-user] Problem setting up admin in Galaxy local instance!

2012-07-20 Thread Daniel Blankenberg
Hi Di, You need to remove the starting comment/hash from the admin_users line: admin_users = dkngu...@uw.edu Thanks for using Galaxy, Dan On Jul 20, 2012, at 5:13 PM, Di Nguyen wrote: Dear all, Please help me figure out what went wrong to set up ADMIN in my local instance using the

Re: [galaxy-user] Error in DepthOfCoverage (GATK) tool

2012-03-02 Thread Daniel Blankenberg
://www.bx.psu.edu/hg/galaxy/ (mentioned in NewsBrief website). I use the first one to update Galaxy. I tried to pull the below changeset, but it says 'invalid revision'. Please suggest. Best, Raj From: Daniel Blankenberg [mailto:d...@bx.psu.edu] Sent: Thursday, March 01, 2012 8:45

Re: [galaxy-user] Error in DepthOfCoverage (GATK) tool

2012-03-01 Thread Daniel Blankenberg
Hi Raj, Thanks for reporting, this issue has been resolved in changeset 6778:35be930b21be. Please let us know if you encounter further issues. Thanks for using Galaxy, Dan On Mar 1, 2012, at 3:30 AM, Praveen Raj Somarajan wrote: Hello, I'm facing an issue with Depth Of Coverage tool

Re: [galaxy-user] Unable to run SICER or Find Peaks

2011-11-29 Thread Daniel Blankenberg
Hi AP, Please keep all replies on list, this will allow the community to assist and benefit from these correspondences. SICER requires BED input. To go from BAM to BED: 1.) Convert BAM to SAM 2.) Convert SAM to Interval (Convert SAM to interval) 3.) Convert interval to BED(6+). This can be

Re: [galaxy-user] Unable to run SICER or Find Peaks

2011-11-28 Thread Daniel Blankenberg
Hi AP, SICER requires BED formatted input with at least 6 columns (for strand information). You can convert your BAM files into SAM and then into interval and BED format. Once you have your input in the BED (6+) format, you should be able to use these tools. Please let us know if we can

Re: [galaxy-user] Question regarding: FASTQ Quality Trimmer

2011-11-28 Thread Daniel Blankenberg
Hi Rahul, This is the maximum number of bases which can have their score not included when calculating the result of the selected aggregation function. For example, if you had a 5 base window with scores of 5,5,2,5 and 5, set aggregation to min score with a specified value of 4, with the

Re: [galaxy-user] GATK

2011-11-17 Thread Daniel Blankenberg
Hi Franzi, You have one too many hg19 in there. The fields go like: unique_build_id dbkey display_name fasta_file_path valid_tool_suites so: hg19hg19hg19 /drive1/galaxy/reference/hg19/sam_index/hg19_ref.fa gatk But do note that these tool integrations are still

Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Daniel Blankenberg
Hi Steffi, GeneTrack should be working again on the Main server, thanks for reporting the error. For information on the inner workings of GeneTrack, you should consult the paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you can additionally contact the GeneTrack author,

Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Daniel Blankenberg
analysis: 19, 20 and 21 cover the usage and principles of GeneTrack (among other topics) http://bcc.bx.psu.edu/courses/597D-2011/index-597D-2011.html best regards, Istvan On Thu, Nov 10, 2011 at 12:19 PM, Daniel Blankenberg d...@bx.psu.edu wrote: Hi Steffi, GeneTrack should be working

Re: [galaxy-user] tool config file: multi-select for files?

2011-10-21 Thread Daniel Blankenberg
Hi Uwe, Multiple selects for dataset inputs is not yet supported, but there is a ticket open on this issue, which you may follow if you like: https://bitbucket.org/galaxy/galaxy-central/issue/137/allow-multiple-true-in-input-param-fields We have not yet decided when-or-if we will be

Re: [galaxy-user] bed12 format for stitching blocks given a set of coding exon intervals

2011-10-20 Thread Daniel Blankenberg
Hi Amit, Without taking a look at your history, I'll have to make a guess. When you retrieve regions from UCSC, on the second step, right before you click Send query to Galaxy, make sure that you have Whole Gene selected under One record per, and that you are looking at a gene track and that

Re: [galaxy-user] Patch for better FASTQ description handling

2011-10-19 Thread Daniel Blankenberg
Hi Florent, Sorry for the delay. I did try the patch out shortly after you contributed it, but it caused the functional to fail. I was able to fix the issue and allow the existing tests to start passing, but I've been bogged down lately and haven't been able to perform a more thorough review

Re: [galaxy-user] Memory issues while using Galaxy

2011-10-18 Thread Daniel Blankenberg
Hi Prashant, Have you set: use_debug = False use_interactive = False In your universe_wsgi.ini file? Thanks for using Galaxy, Dan On Oct 17, 2011, at 6:15 PM, prashant singh wrote: Hi We have installed Galaxy on a local machine (Mac pro 2 x 2.4 gHz quad core Intel Xeon with 32 GB RAM).

Re: [galaxy-user] Regarding perl script

2011-10-18 Thread Daniel Blankenberg
Hi Shambhavi, It sounds like your tool_conf.xml file is malformed. Check that you have proper quotes and that the tag open and close are all correct, etc. There are several programs out there that can validate xml files for you, you may wish to try to validate your xml file with one of those.

Re: [galaxy-user] Chip-Seq, Encode Peaks and Galaxy

2011-09-07 Thread Daniel Blankenberg
Hi Rad, Jorge, Sorry for the delay in reply. We have not yet released a pre-canned workflow to do this. However, if you are looking to associate one set of Genomic interval/region data with another set, Galaxy's interval operation tools are a good place to begin. There are good examples of

Re: [galaxy-user] wiggle file

2011-08-31 Thread Daniel Blankenberg
Hi Rich, Sorry for the delay in reply. You can configure the display of custom tracks at UCSC by clicking on the custom track within the UCSC genome browser. You can also convert your wig file to a bigwig file (under Convert Formats tool menu), in order to speed up the display of this data.

Re: [galaxy-user] history

2011-03-24 Thread Daniel Blankenberg
Hi Tania, In the History pane, Click 'Options' -- 'Saved Histories' and see if your history is listed there. Thanks for using Galaxy, Dan On Mar 24, 2011, at 12:15 PM, Fuchs, Tania wrote: Hello, I worked on my home computer with Galaxy online and next day when I came to the lab I

Re: [galaxy-user] FASTQ to FASTQSanger using Groomer question

2011-03-21 Thread Daniel Blankenberg
Hi David, Your files appear to be of the Sanger FASTQ variant. As you have noticed, the info blurb provided by the Grooming tool provides information that should be utilized to confirm input types. While the 'Illumina 1.3+' FASTQ format does encode scores using a different ASCII range, it is

Re: [galaxy-user] Galaxy on Cloud not recognizing .txt files as fastq

2011-03-14 Thread Daniel Blankenberg
Hi Karl, Using the upload tool for https URLs was added in changeset 5220:425076fe5ea0. I am not sure when this will make it to the Cloud image, but meanwhile, if you can access the file without using ssl (just 'http'), you should be able to upload. If this is not the issue you are

Re: [galaxy-user] Import data in RGenetics

2011-03-10 Thread Daniel Blankenberg
Hi Sylvain, This issue has been fixed in changeset 5207:72d560d3e7fd and will be available the next time that the main server is updated, which should be within the next few weeks. Thanks for reporting this error and please let us know if we can provide additional assistance. Thanks for

Re: [galaxy-user] FASTQ type change

2011-02-17 Thread Daniel Blankenberg
Hi Stephen and Peter, This change is definitely possible, and because the SAM format specifies that its quality scores are phred scaled and ASCII offset of 33 (regardless of provided input) it shouldn't cause complications downstream. We'll add this to our todo list. I created a ticket: