Re: [Histonet] Histonet Digest, Vol 245, Issue 17

Ann Specian (where?) asks about Epredia Xylene Substitute: >>We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene?substitute that they could share?<< Well, what’s in it? You’re asking your readers to look it up for you. I

Re: [Histonet] Control Slides (Ken M)

Here's the opinion of an 85 year old pathologist who in the course of his training spent a year doing histochemical research. In my experience, pathologists aren't allowed much input in the selection of controls. Anyone doing special stains needs to be able to evaluate the control slides for

Re: [Histonet] Histonet Digest, Vol 243, Issue 7

> > Re: [Histonet] Modified Davidson > > The formula for "modified Davidson's fixative" that I used was three parts tap water, three parts reagent alcohol, two parts 37% formaldehyde ("strong formalin"), one part glacial acetic acid. Best mixed under a fume hood. At 85 I must be one of the last

Re: [Histonet] Faded H tissue section

> > > I recall learning the technique of using 1% periodic acid to brighten up > dismal hematoxylin staining, when I was a resident at Johns Hopkins in > 1970. I don't know where the histochemist I learned it from got it, though Bob Richmond Maryville TN

Re: [Histonet] Coffee at the desk

John Kiernan asks: > > >>Bob, Why weren't they routinely buffering (or at least neutralizing) the > formalin fixatives at Johns Hopkins as recently as 1970? - It had all been > in the scholarly books (by Pearse, Lillie, etc) for >10 years, and was also > in Lee Luna's 1968 Manual of Histologic

Re: [Histonet] Coffee at the desk

This 84 year old pathologist recalls the histopathology laboratory at Johns Hopkins Hospital around 1970, when I was a pathology resident there. Histotechs, often laboratory clerks, sat in front of rows of 400 mL Stender dishes, smoking cigarettes while they hand-stained slides, often carrying

Re: [Histonet] Sudanblack B on FFPET

> > Gudrun Lang in Austria asks: > >>Has anyone experience with Sudanblack B on paraffin slides for staining [lipofuscin]? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night.

Re: [Histonet] The Passing Of Dr. James McCormick

> > > I used a cryostat, a large, very primitive cryostate (long fleece gloves) > at Washington University in St. Louis in 1962, in a laboratory doing the > quantitative histochemistry techniques developed by Dr. Oliver Lowry before > then. We cut very thick sections, around 50 or 100 µm as I

Re: [Histonet] Cryostat anti-roll plate

> > > My experience with anti-roll plates is mostly with the old International > cryostats of days gone by. Pathologists usually disdained anti-roll plates > and used an artist's brush to keep the section from rolling, but more > proficient users depended on them. I learned to use them in a

Re: [Histonet] Dr. Freida Carson

> > > I was surprised that Dr. Freida Carson doesn't have a Wikipedia bio. Can > somebody take care of that? Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] How To Store Fresh Frozen Tissue Over a Weekend

> > Christie Saunders MLS(ASCP)CMQIHC, MOHS Histotechnician in Ohio, asks: > > >>I may have a silly question, but I truly do not know the answer to this > question. I work in a MOHs lab and need to know if there's a particular way > to store unprocessed tissue in a fridge or freezer in some way

Re: [Histonet] Cutting protocol for gender dysphoria

> > Victoria Spooner at Bassett Healthcare in New York state asks: > > >>Does anyone have cutting protocols for mastectomies for gender dysphoria > they would [share] with me? How many blocks are submitted per breast?<< How > do they compare [to] mastectomies for tumor?<< > I don't know of any

Re: [Histonet] release of body parts

Patients should not be given formalin. You can transfer the specimen to 70% alcohol, and hope they don't drink it. If you're in one of those unusual pathology services where photography is permitted, I wonder if you could offer the patient a photograph of the specimen by e-mail. Bob Richmond

Re: [Histonet] Frozen section problem

Dorianne Bonello, Allied Health Practitioner (MLS), Histology Laboratory - Pathology Health-Mater Dei Hospital, on the island of Malta asks: > >>We are experiencing freezing artifacts on our frozen sections. > Basically, we are seeing cavity-like structures under the microscope, > mostly

Re: [Histonet] Formalin question

> > Anne Murvosh HT at Advanced Dermatology asks: >>I was wondering if 10% > formalin needed to be kept in a flammable cabinet. We never have before, > but I wondered if we were doing it wrong or if regulations have changed.<< > > Formalin and neutral buffered formalin (3.7% formaldehyde in water)

Re: [Histonet] Bouin's fixative

> > Bouin's fixative has no business being used in a spray bottle. It's a very > toxic material that shouldn't be aerosolized. > As Dr. Steve McClain suggests, dilute acetic acid - 3 to 5%, white vinegar, is quite sufficient for preparing tissue for inking, and isn't toxic and messy like Bouin's

Re: [Histonet] Non-Pathologist Grossing (Amy Self)

Vikki Baker (or Amy self) - where? - asks: > >>We recently had an incident which led us to review our policy on frozen > section QA. In your institution does the same pathologist who did the > frozen section read the permanent [paraffin] sections? Also if they do > then who is the pathologist

Re: [Histonet] human shoulder joint fixation

> > Merissa (where?) asks; >>I am doing some planning for a new project and > wanted to get opinions on fixation of large pieces of tissue. We will have > human shoulders, where we want to preserve the rotator cuff/joint. Cutting > the tissue with a saw will damage the soft tissue, so we were

[Histonet] AFIP Manual

> > The edition of the "AFIP Manual" - Manual of Histologic Staining Methods > of the Armed Forces Institute of Pathology, Third Edition - most of us are > familiar with was edited by Lee G. Luna. I recall buying a copy of it in > 1968 when I was a resident. I now have a copy of what I think is

Re: [Histonet] Gram staining

> > In answer to a query about tissue Gram staining, Tony Henwood adds > instability of the iodine solution to the long list of reasons why tissue > Gram staining doesn't work very well. > The Gram stain done on smears is of course one of the most useful microtechniques in microbiology, but

Re: [Histonet] Levels on biopsies

> > Brad Ringer, HTL (ASCP). histology manager at Springfield Clinic in > Springfield IL asks: > >>I'm curious to know how many levels your lab cuts on the following > biopsies: prostate core, cervical, vaginal, bladder, and anus biopsies. I > ask this because we are currently (and have been

Re: [Histonet] Recommended thickness of Amyloid sections

In response to several questions and responses about amyloid staining: I’ve always understood that sections for amyloid should be cut at 8 µm, but could never get a histotech to cut them that thick. I recall that unstained paraffin sections on the slide are usable for about a month after

Re: [Histonet] Question concerning H. pylori staining

Michelle (where) asksi: >>We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate, Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol

Re: [Histonet] Glycogen detection

Galina Deyneko (where? asks: >>Does anybody have experience how fix the tissues for successful glycogen ? detection in murine and humane cardiomyocytes. I am wondering maybe the trace of methanol in 10% formalin will dissolve glycogen?? - What would be better process for paraffin embedding or use

Re: [Histonet] Incomplete cross sections of all tissue in blocks

Steve A. McClain, MD at McClain Labs in Smithtown NY notes: >>...to avoid incomplete sectioning...: ink the specimens well, using acetic acid (vinegar) to fix the ink, thereby making the ink easier to see in the block.<< I don't like ink for this purpose, because it clutters up the microscopic

Re: [Histonet] Giemsa controls

Tanya G. Abbott, Pathology Manager, PennState Health St. Joseph in Reading, Pennsylvania asks: >>Suggestions for where to buy good Giemsa controls for H. pylori?< What I've always found satisfactory is to watch for positives in your own material, evaluate them as possible controls, and use

Re: [Histonet] Cellient for cell block preparation

Richard w. Cartun, MS, PhD, at Hartford [CT] Hospital asks: >>What has been your experience with Hologic's Cellient instrument for preparing cell block specimens? I understand that it is very good for pauci-cellular specimens. However, I am concerned about using these preparations for

Re: [Histonet] Changing to reagent alcohol

Carole L Johnson, HT(ASCP)cm, QIHC(ASCP)cm at UCH/Memorial Central Hospital in Colorado Springs asks: >>We have been using ethanol in our lab and would like to change to using reagent alcohol for the obvious reasons of cost and regulatory headaches. I have been tasked with creating a

Re: [Histonet] Weekend Coverage for Small Pathology Labs

Scott A. Lindrud, MLSCM(ASCP)CTCM, Histopathology Technical Specialist at Carris Health in central Minnesota (apparently not part of the Mayo Clinic system) asks: >>I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs?

Re: [Histonet] Extremity carrier

Dawn Olszewski asks: >>I have been tasked to find a better solution to transport extremities from the OR to histology. We have tried cardboard boxes (no longer allowed) and plastic totes on rollers (too big to store and absorb odors from the legs).<< Mopec offers >>Pathport 3 is a stainless

Re: [Histonet] Histo techs assisting on BM Bx's

I usually had hematology technologists assisting me back when I was doing bone marrow biopsies long ago. I trained more technologists than I remember. You always have to remind the patient that you're going to be talking a lot because you're training a new assistant, but that it isn't going to

Re: [Histonet] Freezing Sprays in Cryostats

Freezing sprays for frozen sections in cryostats are deplorable, but try and get pathologists to give them up. I think that's probably why the CAP has been reluctant to ban them. During my career in pathology I saw more than one case where frozen sections were inadvertently cut on tuberculous

Re: [Histonet] Mallory-Azan stain

Betsy Molinari HT, ASCP at the Texas Heart Institute in Houston asks: >>I have a researcher that wants to stain Purkinje fibers and has requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice.<<

Re: [Histonet] Changing from HemoDe to VWR Xylene Substitute?

Michelle (Shelly) Aono (at Auburn in Alabama) asks: >>Does anyone have any experience changing from HemoDe to VWR Xylene Substitute? Did you have to extend your processing times? By how much? When people talk to good "recycling" of the clearing agent, does that mean re-use? As in how often you

Re: [Histonet] Victoria blue for lung tissue

Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers

Re: [Histonet] fume hoods for grossing

Mehndi Helgren at Dominion Pathology Laboratories in Norfolk, VA asks: >>I'm trying to see what kind of exhaust/fume hoods people are using in small grossing labs. We are moving into a new space and I want to make sure the ventilation at the gross board is adequate. I don't think an actual

Re: [Histonet] Benchmarks for reprocessing

Cristi Rigazio asks: I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable.<< This grumpy old pathologist sez: If I'm the one doing the grossing, I

Re: [Histonet] biopsy staining

Betsy Molinari, HT (ASCP) at the Texas Heart Institute in Houston asks: >>I have some very small pieces of tissue that are in 70% ethanol. Should I mark them with eosin or safranin O? From what I have read the tissues were still in formalin, and mine are already in alcohol. Can they still be

Re: [Histonet] 5% Chromic acid disposal

Sharon at Celligent Diagnostics in Spartanburg SC asks: >>We are changing our GMS stain over from a Periodic acid kit to a 5% chromic acid kit. What do the labs that use chromic acid in special staining do with the waste/ used chromic acid?<< I hope someone can give an authoritative answer to

Re: [Histonet] Xylene free processing

Cindy McGrady at Hurley Medical Center in Flint, Michigan asks: >>Our laboratory is experimenting with xylene free processing on our Sakura VIP, substituting isopropanol for our xylene substitute. Our pathologists have complained about processing, especially the biopsies, with our regular

Re: [Histonet] Words, phrases and names in histotechnology. A free glossary.

the years > > Respectfully, > > Colleen Forster > U of MN > > On Wed, Feb 13, 2019 at 4:35 PM Bob Richmond via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > >> John Kiernan, certainly glad to see you back on HistoNet! - At 80 I've >> f

Re: [Histonet] Words, phrases and names in histotechnology. A free glossary.

John Kiernan, certainly glad to see you back on HistoNet! - At 80 I've finally retired. This Biological Stain Commission glossary is a really useful resource I wish we'd had long ago. A lot of information in it difficult to get elsewhere. I'll link it on Facebook and on Sermo. Two suggestions:

Re: [Histonet] ER/PR question

Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco, CA asks: >>One of my pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay?<< You can only try. There

Re: [Histonet] Opinion on dye on biopsies

4, 2019 at 6:40 PM John Garratt wrote: > Be aware that validation of IHC should be performed if you are changing > your processing protocol by adding a dye to the reagents or to a > pre-processed tissue. Be cautious! > > > John > > > > On Saturday, January 12

Re: [Histonet] Eosin on processor for biopsies

Gareth Davis asked about marking small GI specimens with dye when grossing them. I've used safranin O - the solution the microbiologists use in the Gram stain. If you walk down the hall to the micro lab you can get a small amount to try out. Do not use eosin for this purpose. Eosin's brilliant

Re: [Histonet] Opinion on dye on biopsies

Gareth Davis asked about dyes to use to mark small GI biopsy specimens to make sure they're recovered during embedding. I've had good results marking small specimens with the solution of safranin O that's used in the microbiologists' Gram stain. Go to the micro lab and ask for a small amount of

Re: [Histonet] Grossing large ellipse melanomas

Anne Murvosh HT at Advanced Dermatology & Skin Surgery in Washington state asks: >>We are starting to keep our melanomas in-house now that we have a better processor, however I could use some tips or tricks on cutting large fatty skin ellipse specimens. My problem I think, is too small a knife,

Re: [Histonet] Breast lumpectomy fixation

Charles Riley BS HT, HTL(ASCP)CM, Histopathology Coordinator/ Mohs (where?) asks: >>How does everyone gross and process your breast lumpectomy / mastectomy specimens?<< These are extremely complex and exacting procedures. It's your pathologist's job to do this, and your pathologist can't

Re: [Histonet] Policy bariatric patient for autopsy

nal health policy. Institutions may also choose whether to use > special equipment for > such patients and what type(s) of equipment to use. > Evidence of Compliance: > ✓ Written policy for handling of bariatric patients > > ____ > From: Bo

Re: [Histonet] Policy bariatric patient for autopsy

I don't understand Lorraine Smallwood's question: >>Can anyone give me info on the CAP inspection packet regarding policy for bariatric patient for autopsy? I am a new "Histonet" junkie so any help from the experts will be greatly appreciated.<< CAP inspections don't concern themselves with

Re: [Histonet] Bouin's Fixative Substitute

Linda Miller posts in what appears to be an advertisement: >>New! Bouin's Fixative Substitute is a low-hazard replacement for Bouin's Fixative. This substitute provides all the benefits of Bouin's without the use of picric acid. Available in a variety of sizes and pre-filled specimen

Re: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help!

Pam Barker issues one more of her frequent appeals for histotechnologists. I can't remember when I last heard of a histotechnologist looking for a job. >From the perspective of a retired pathologist in his 80th year: we'll get enough good people in histotechnology when we start paying

Re: [Histonet] Giemsa vs Diff Quik

Joseph A. Esposito at McClain Laboratories on Long Island asks: >>The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This

Re: [Histonet] Calretinin for Hirschsprung's

Chris Mason (where?) asks: >>Would anyone be willing to share their protocol for the sectioning of Hirschprung's cases and the use of calretinin? Our pathologists want to begin using this technique. We are thinking of alternating H and unstained to be used for calretinin in the case of

Re: [Histonet] Iron Stain

Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in Frederick, Maryland asks: >>Is nuclear fast red the only counter stain for the Prussian blue stain? I have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could be [used as] a counterstain. I won't be

Re: [Histonet] Quality Measure for Pathology

Amy Self, Histology Lab Senior Tech at Tidelands Georgetown Memorial Hospital in Georgetown SC asks: >>I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard.<< Look at the August 2017 number of CAP

Re: [Histonet] Warm up freezer-stored powder, or use right away?

Tim Morken (Pathology Site Manager, Parnassus - Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology - UC San Francisco Medical Center) asks: >>We have a debate going on for those freezer-stored powdered chemicals used for enzyme histochemistry. One side says warm

Re: [Histonet] procedure for peripheral nerve damage

Cheryl Kerry, HT(ASCP) in Houston TX asks: >>A client wants us to start reading peripheral neuropathy biopsies. Hoping I don't have to start from scratch - anyone have processing and prep procedures including cutting protocols?<< This is a highly complicated procedure, often involving

Re: [Histonet] Histonet Digest, Vol 174, Issue 9

Jamie Watson, you need to clarify this question: >> Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor?<< 100% ethanol is perfectly OK, but it requires a lot of regulator compliance because you can drink it (though you

Re: [Histonet] Congo Red

Carl Hobbs FIBMS, Histology and Imaging Manager at Kings College in London replies: >>That's a good point, Bob (mouse amyloid controls for human sections). The amyloid produced by the the various mouse models (TASTPM, TGs) is human amyloid... as you know. I suspect that it wouldn't be accepted as

Re: [Histonet] Wright-Giemsa for sections?

Tyrone Genade in Orange City, Iowa asks: >>Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections? Does anyone have a protocol? I want to examine hematopoietic tissue of fish, i.e. the head kidney. No smears or imprint possible. I would like to use Wrights so I can use the same

Re: [Histonet] Pathology Charges

Amy Self, Histology Lab Senior Tech, Tidelands Georgetown Memorial Hospital in Georgetown, SC asks: >>I am working on our billing process in hopes to improve things and make it a little more efficient for us. We really have room for improvement. I feel there is lots of work that is being done

Re: [Histonet] Cross Contamination CAP policy

Terri L. Braud, HT(ASCP), Anatomic Pathology Supervisor at Holy Redeemer Hospital in Meadowbrook PA notes: >>Our policy calls for wiping of forceps with gauze between cases at gross and at embedding. At gross, we use a disposable absorbent lined pad on the cutting board for each larger case, and

Re: [Histonet] Modified GMS Protocols

If you choose another oxidizing agent than CrO3 (such as KMnO4, potassium permanganate) it's up to you either to find literature to back you up, or to do the stain with appropriate controls for the fungus you're looking for. Freida Carson (citation below) found that periodic acid, commonly

Re: [Histonet] Prostate needle biopsies

Martha Ward at Wake Forest Baptist Health, Winston-Salem, North Carolina asks: >>I am posting this question for our Histology manager. For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the

[Histonet] Obituary - pathologist Bernard Leon Klionsky

With his permission I post pathologist Leon Metlay's Facebook obituary of his wife's father: My father-in-law, Dr. Bernard Leon Klionsky, died [November 11th, 2017] at the age of 92. He was one of the unsung heroes of pathology. As a young man, he invented the form of cryostat that we all use to

Re: [Histonet] Histonet Digest, Vol 167, Issue 22

The old Samurai Pathologist has a copy of Ann Preece's book. I was contemptuous of it as an arrogant young pathologist of 28, deeply respectful of it as an arrogant old pathologist of 78. But it's a reproach that a fifty year old book has not been superseded. Bob Richmond Samurai Pathologist

Re: [Histonet] Amputated Limb Storage

Amy Self, Histology Lab Senior Tech, Tidelands Georgetown Memorial Hospital, Georgetown SC asks: >>How is everyone storing their amputated limbs that are received in pathology? Are they stored in the morgue or in a refrigerator located within the department and at what temperature is suggested

Re: [Histonet] Blood donations for money

Elizabeth, I certainly wasn't talking about volunteer platelet donors, a totally different, very dedicated bunch. I would continue to observe that plasma centers are located in fairly sleazy parts of town. I wish I could continue to be a donor - whole blood, because I'm a rare donor (group O, and

Re: [Histonet] Blood donations for money

I had no idea that there was a shortage of immune globulin. When I was with Red Cross in the 1970s, we were awash in it, though only a few batches were usable IV, a technology that was just developing then. If we disrupt the health care payment system, the plight of our hemophiliacs will be

Re: [Histonet] Blood donations for money

Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose he's in the US. I don't think there's any paid donation of whole blood in the US any more. This is probably a plasmapheresis center, where people donate twice a week. The red blood cells are returned to the donor. Two

Re: [Histonet] Clear-Rite 3

Richard W. Cartun, MS, PhD - Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory - Director, Biospecimen Collection Programs - Assistant Director, Anatomic Pathology, Hartford Hospital, Hartford, CT asks >>Anyone using ClearRite (xylene replacement)

Re: [Histonet] Coagulants question

From: jdhanna...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coagulants question Message-ID: Content-Type: text/plain; charset=us-ascii "jdhannasch" (who) asks: >>Can someone explain to me what a coagulant vs a

Re: [Histonet] blades

Lauren Sweeney at the University of Georgia asks: >>Blades for grossing tissues- anyone have recommendations? The vendor that we were purchasing just straight razor blades from has stopped manufacturing them. We don't use scalpels because of their higher cost and we are only working with poultry

Re: [Histonet] Disposal of Bouin's Solution

Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd

Re: [Histonet] Microscope selection

Warda Hassan (where?) >>would like to have some advice from expert from the field of pathology on microscope selection. Which one would you select as a manufacturer for microscopes?<< Speaking from more than 50 years of going to and fro in the world of pathology, and walking up and down in it

Re: [Histonet] Hirsch-Pfeiffer cresyl violet method

Tim Morken in pathology at UC San Francisco Medical Center asks about the Hirsch-Pfeiffer (correct spelling) cresyl violet stain for frozen sections. "I've found many references to it, but none that give the procedure. And the original paper is from 1955 not easily available. And is in German."

Re: [Histonet] Processing cystic tissue

Charles Riley HT, HTL(ASCP)CM, a Mohs histopathology coordinator, asks: >>Can anyone give me an idea how they process cystic tissues? The normal tissue processes extremely well on my current protocol but the cystic areas just are too soft to cut. Any tricks anyone can provide to process better or

Re: [Histonet] Tissue fixation

Tim describes a problem "I have a pathologist that is not happy with the fixation on some of our LEEP specimens." LEEP specimens are inherently crappy, because of the cautery used to obtain them, and the resulting cautery artifact in the specimens. In the last few years they've turned the voltage

Re: [Histonet] solvent recyclers

A few years ago the B/R spinning-band stills were definitely the still of choice for recovering histologic solvents, and the older generation of CBG machine were inadequate. I think though that the later generation of CBG stills predominates today. I don't know how they work. Could somebody give

Re: [Histonet] Use of sodium borohydride in immunofluorescence

> > Lucie Guernsey at UC San Diego asks about sodium borohydride: >>I'm currently going over IHC SOPs that were written by lab members that have long ago moved on to other jobs. One inconsistency that I've come across is the use of 0.5 % sodium borohydride in immunofluorescence.<< Does your

Re: [Histonet] Happy Histotechnology Professionals Day!

The old Samurai Pathologist - now retiring at 78 - thanks the many histotechnologists who've kept him out of hot water the past 52 years. Too bad we can't have more kids in search of a career reading Histonet - or at least, the Help Desperately Needed notices! Bob Richmond Samurai Pathologist

Re: [Histonet] Eosin B solution for H

Gudrun Lang (in Austria) asks: >>I wonder how widespread the usage of eosin B is in H protocols. Is there a specific difference in application to Eosin Y?<< Eosin B is closely related to the more commonly used eosin Y. As the letters imply, it's Bluish rather than Yellowish. I've never seen

Re: [Histonet] Breast specimens

Charles Riley HT(ASCP)CM asks: >>What is the best way to handle breast specimens that were grossed too thick and did not process well? Our medical director does not want us to reprocess the tissue but it is almost impossible to get even a remotely decent section. If anyone has any other tips

Re: [Histonet] Harris Hematoxylin

Jessica Piche, HT(ASCP) at Waterbury Hospital [Connecticut] asks: >>Just wondering what kind of Harris Hematoxylin people are using. We are doing a regressive H on a Leica Autostainer XL for 9 minutes. We have been buying Ricca Hematoxylin but we are having so many issues with it. Are people

Re: [Histonet] Bone saw

> > Terri L. Braud, HT(ASCP). Anatomic Pathology Supervisor, Holy Redeemer > Hospital, Meadowbrook PA describes: > > >>We use an awesome little band saw made by IMEB, Inc. It has a small > foot print, 4 blade types and added accessories for a super lab bone > cutting station, and best of all,

Re: [Histonet] Magnifiers for Embedding

>>Lisa Hamilton asks: In the past I have seen clip-on magnifiers that are used to embed tissue. Of course now that I need one I cannot remember what catalog I saw them in! I was hoping someone might.<< If you're looking for something that fits over your head and puts lenses in front of your eyes

Re: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization

Further researching the topic, I've learned that there are two recently introduced non-radioactive alternatives, Savi Scout and Sentimag. I don't understand how they work. Coincidentally, I received a circular in this morning's mail with information about a continuing medical education event on

Re: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization

Jim Vickroy, Histology Manager at Springfield [Illinois] Clinic asks: "Our organization is looking into the workflow necessary to handle breast lumpectomies with radioactive seed localization (RSL)". Thanks for the heads-up - as usual, pathologists and histologists don't get told. Nursing

Re: [Histonet] Modified Movat's Pentachrome stain

Angela Lamberth at the La Jolla [California] Institute for Allergy & Immunology asks: >>I'm gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I've found a protocol used by the Children?s Hospital of Philadelphia Pathology

Re: [Histonet] Eosin

Elizabeth M. Cameron, HT(ASCP), QIHCCM. Lead Histologist at Mid Coast Hospital in Brunswick, Maine asks: >>I have been staining fish tissues fixed in Davidson's fixative with H, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but

Re: [Histonet] Gallocyanine

Here's John Kiernan's extremely informative review of gallocyanine (that's how he says it's to be spelled) from the archives - I can't find a date on it. Really tells you everything you need to know to do Nissl staining with it.

Re: [Histonet] Gallocyanin

Liz Chlipala at Premier Laboratory in Boulder CO writes: >>We have run this stain [gallocyanin] before, purchased the reagent from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble.<< What stain? What mordant (chrome, aluminum, none)? To stain what? I think the person

Re: [Histonet] Gallocyanine

Eva Permaul at Georgetown University asks: >>We just had a request for Gallocyanine staining. Does anyone do this? Can you share your protocol? Where do you buy your reagents?<< Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used

Re: [Histonet] B-5 Fixative

Jillian A. Russell, HT (ASCP)CM, QIHCCM, Supervisor, CDx Histology Operations, R at Dako in Carpinteria CA asks: >>I am wondering how many labs are using B-5 fixatives and how many are using B-5 alternatives due to the mercury issue? - For those who have switched to an alternative, have you

Re: [Histonet] 360 video of specimens

Steve A. McClain, MD asks: >>The next 3 weeks we are turning our lab cameras and our specimens toward making a 360 photographic orbits around specimens to create a 360 stitched image or video. If you know any pathologists who have tried 360 imaging around a specimen, kindly send me their name. I

Re: [Histonet] dye in processor

Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?" Safranin O - the solution your microbiology

Re: [Histonet] Histonet Digest, Vol 152, Issue 13

Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?" Some time ago I worked in a lab that used

Re: [Histonet] Marking Tissues with Eosin

Rebecca Ashley at the Wyoming State Vet Lab in Laramie asks >> I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block. I've heard mention of marking these with eosin to make them easier to see. Has anyone done this? Or do you use some other type of

Re: [Histonet] PAS Stain

s. > > Why not just standardize it from the start, reagent, pH, temperature and > it really cannot fail. > > Spokane Ray > > -- > *From: *"Bob Richmond via Histonet" <histonet@lists.utsouthwestern.edu> > *To: *"Histone

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