ave to say it was prominent in our careers. It was where I
was first exposed to Histology. I have enjoyed your friendship and comradeship.
Take care
Victor
Sent from my iPad
> On Sep 9, 2021, at 9:26 AM, Morken, Timothy via Histonet
> wrote:
>
>
> After 40 years in the lab I've de
After 40 years in the lab I've decided to retire this year - in a week actually!
It has been an interesting 4 decades...
I started out in an EM lab after getting a degree in Physiology and then
competing a 2 year EM course at Delta College in Stockton, CA - the only
dedicated EM program at
Can anyone supply me a copy of this paper on epoxy resins? I can't find it
anywhere on line and our library does not have that journal.
Glauert AM: Epoxy Resins: An update on their selection and use. Microscopy and
Analysis 15-20 Sept 1991.
Tim Morken
Supervisor, Electron
Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two
Epredia NX70 cryostats. We've used them for 8 years now and really like them.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Can anyone give me any tips on EM immuno-gold labeling for kappa and lambda
light chains in amyloid cases? We use Eponate12 for embedding.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Curt, You can make and use barcodes in MS Excel or Access and print labels. If
you keep the clients items together in some kind of bag or container you can
put the label on the outside and scan at each station to track them. You just
need to be sure the items are all kept together to ensure
Beth, which C4d are you using? And it is a FITC-labeled primary?
We use it as a two-step rather than FITC-labeled primary. That increases the
sensitivity significantly. Gloms have C4d so act as an internal control.
We use
C4d antibody, 100ul vial, unlabeled primary. Diluted 1:200 in Dako
London, Canada
= = =
From: Morken, Timothy via Histonet
mailto:histonet@lists.utsouthwestern.edu>>
Sent: May 28, 2021 11:31 AM
To: Histonet
mailto:histonet@lists.utsouthwestern.edu>>
Subject: [Histonet] IF with permanent mounting media?
Has anyone tried usin
months.
I realized I did not reply to the histonet the last time, I still need to get
myself familiarized with this : (
John Lai
Posdoctoral Fellow, School of Life Science, HKUST
-Original Message-
From: Morken, Timothy via Histonet
Sent: Friday, May 28, 2021 11:31 PM
To: Histonet
Has anyone tried using xylene/permanent mounting media for immunofluorescence
stains? I had a question from a pathologist who wondered if we could do this. I
have never heard of anyone doing it.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC
-Original Message-
From: Morken, Timothy via Histonet
Sent: Wednesday, May 19, 2021 8:26 AM
To: Brittany Hethcox
Cc: Histonet
Subject: Re: [Histonet] Cryostat
Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're
reliable and easy to use. We have the elevator on ours
Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're
reliable and easy to use. We have the elevator on ours and it makes a
difference for different size people in the lab. It also can have a vapor
disinfectant system which we use and it works great.
We use ours for
Jennifer, we have used the Sakura Glas, Glas2 (attached to prisma stainer), and
now the Sakura tape cover slipper attached to the Prisma. Also we have had the
Leica CV5030 glass coverslipper.
All worked as long as they are kept clean. Daily maintenance is a must. If in a
service contract they
Jessica, CAP and JC apply the same CLIA regulations, just in a different
manner. CAP tends to apply more of their own "upgrades." Ie, makes it a bit
stricter, which is allowed. CLIA is just the baseline. If you have been thru
CAP inspections you will not have any problem with JC.
While CAP
Maria, we went back to using Dako Diluent, which we had just switched away from
to save money!
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Maria Cruz via Histonet
Sent:
Those labs doing diagnostic immunofluorescence for kidney or skin, do you put
in the IHC disclaimer used for peroxidase staining on the reports?
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Hi all, I'm going to give a presentation on online histology help sites like
Histonet and the NSH Block. Does anyone know of other listservers that can be
of help to histotechnologists in all fields? I checked the MSA EM listserver
but it is down for some technical reason.
Thanks in advance
Hi all, I was wondering if anyone has used the Milestone PrestoChill for
freezing muscle for histochemistry.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
___
Histonet
Amy, all our orders are thru our LIS, Copath Plus. Residents or pathologist
order on their own. Except for grossed cases. They get standard panels ordered
upon accession or grossing.
Outside cases are all consults. Some are routine (for instance our kidney lab
where we do routine panels) so
Our source for pre-made chrome alum-subbed slides does not exist anymore. Does
anyone know of a company that produces these?
(American Master Tech had them but when StatLab bought them they discontinued
the chrome alum slides)
We used these for EM thick sections because they hold a water drop
I’m in central PA
Sent from my iPhone
> On Jan 8, 2021, at 3:03 PM, Morken, Timothy wrote:
>
> Dee, it depends on where you are
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology UC San Francisco Medical Center
>
> -Original
Dee, it depends on where you are
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Dee Neamand via Histonet
Sent: Friday, January 08, 2021 11:47 AM
To:
Amy, they don't have a specific AP and General checklist like CAP has. They
have a Standards book that lists all the requirements and you need to go thru
and figure out which apply to your area. It is also on line at the TJC site.
The thing is that TJC covers the entire hospital, not just the
Martha, we give it the same number sequence as any other surgical or cytology
case, but we can identify our cases by "Specimen Class" so consults get a
specimen class according to the type of consult - cyto, cytogyn, surgical,
etc. The specimen class is printed on the labels for all
Kelly, You are not going to stay within the temp ranges if putting pellets
directly into the embedding center tank. A couple solutions: 1) write into your
procedure that the temperature will go down when pellets are added and that
once melted should be in whatever range it calls for (and don't
Manahil, we do IF on paraffin sections on occasion when kidney tissue is
insufficient (and then only IgA, G, M and K, L) or they want the markers on
tissue that is only embedded in paraffin, such as kappa, Lambda for amyloid. We
use at a higher concentration than for frozens (1:10 vs 1:100)
Amy, we have been using Awarepoint, but they are apparently going out of
business so we are switching over to Stanley Aeroscout on all fridges, freezers
and room temp/humidity throughout our facilities.
We have direct access to the monitoring of each "asset" and can check it online
at any
Nancy, we have HT (Histotechs) and HLT (Hospital lab techs - lab assistants)
who work in grossing and do the frozen sections with residents and
pathologists. There is no requirement that a person cutting sections has to be
certified in anything. Our HLT's generally do all the accessioning but
" The way I read it, the only things in pathology that are actually high
complexity testing that aren't performed exclusively by pathologists are
and QCing special stain/ISH/IHC slides (ANP.21395). "
CLIA regulations specifically state that quality control is not a test so it is
not
When we implemented barcoding I was planning on using direct slide printing. It
seemed the most efficient and safest way (ie, no mislabelling) to do the
labeling.
However, when we looked into getting the printers they were on the order of
$9,000 per printer. And we wanted 20 printers for
Greg, thanks!
I have a leica engineer coming in to day to look at it, specifically the
fluidics system, so hopefully he’ll see what you mention and fix it.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
From:
g a
surfactant.
Extreme solution: place solutions in a vacuum.
Sent from Smallbiz Yahoo Mail for iPhone
On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet
mailto:histonet@lists.utsouthwestern.edu>>
wrote:
Has anyone solved a problem on the Leica Bond stainer of bubbles ov
Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue
sections that cause unstained (ie, clear) areas where bubbles have formed?
We've been trying things like extra buffer wash before reagent, new slide trays
(tech rep suggestion). It is seen on single- antibody slides
Steve, I agree with your whole thing. And our pathologist fought against
plastic for years simply due to the fact it scratches easily and we are
constantly pulling and filing slides for various needs. However, when we
decided to go to scanning slides wet mounting media was never going to work -
Hi Jeanine!
We are using film coverslipping exclusively now and scanning all slides. We
moved to film because it dries almost instantly and can be scanned right away.
It has worked very well. The only issues, and not specific to film
coverslipping, are that some slides with low contrast
I neglected to mention sections should be cut at 10um for best results.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Morken, Timothy via Histonet
Sent: Tuesday, July 28, 2020
Ken, Yes, polarized light and apple green birefringence is diagnostic for
amyloid with congo red and is the best practice. If you have a problem with
known control slides there are two possibilities: 1) make up fresh solution.
The pH has to be right. Or 2) try other control slides. Maybe you
Samantha, Yes there is:
Staffing Benchmarks for Histology Laboratories
Rene Buesa
Annals of Diagnostic Pathology
Volume 14, Issue 3, June 2010, Pages 182-193
He details here how lab assistants make labs more productive by allowing
trained histotechs to do the embedding, sectioning and staining
Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under
polarized light brighter. Also the deposits can be variable so even with the
light microscope the reddish deposits will stain stronger with thicker
sections.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular
Something for others to know about
https://www.linkedin.com/posts/activity-6653762952572788737-jqh8
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
___
Histonet mailing
Here's a question that came up. A stand-alone EM lab at a college that is
associated with a medical center, but in a different city, wants to do the EM
workup for the medical center. But they would only take in samples, do the EM
processing and take images then the pathologist would read the
Posted for a friend...
To my knowledge the best institutional manual is the University of Michigan's
Cutting Manual.
-Izak Dimenstein
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
The problem with xylene is that the acceptable air level in the lab is 100ppm
but humans can detect it by smell at the 5 - 20ppm range. So it seems like it
is "everywhere" but it could still be at a very low level. What level is safe
for a pregnancy? CDC has some info on this:
This is a secure message. Click here
https://pe-002e8f01.gslb.pphosted.com/formpostdir/securereader?id=1VTCLvZhK2OwIXUVqkgYRCeLHWKdc-FL=66d9f965
by 2019-12-18 09:14 PST to read your message. After that, open the
attachment.
[Image?c=logo=1=2=3.1510941425865]
This is a secure message. Click here
https://pe-002e8f01.gslb.pphosted.com/formpostdir/securereader?id=TSHBjjhKZJQbAmmmM9ouoE388nfx0p00=66d9f965
by 2019-12-18 09:03 PST to read your message. After that, open the
attachment.
[Image?c=logo=1=2=3.1510941425865]
Paula, we use Cerner Copath Plus and scan into the case all requisitions and
any other paperwork at accessioning. The person grossing uses the barcode on
hard copy requisition to access the case but the hard copy stays in the gross
room so no contaminated paperwork (or any paperwork at all)
I'm wondering how other muscle histochem labs handle their workflow. Because
the histochem stains require frozen sections and a freshly-prepared reagents,
and muscle conditions are not usually very time sensitive, we batch all our
cutting and staining to one batch per week. We have 5-10 cases a
I wonder if it is just a coincidence that and ad for this device from Milestone
came thru in the middle of this freezing spray string
https://www.milestonemedsrl.com/us/product/prestochill/
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
You can see past issues of Morbidly and Mortality Weekly Report (MMWR) at:
https://www.cdc.gov/mmwr/index2019.html
For past issues, click on "weekly report" on the left hand side and you can get
to a list of past issues.
However, for the publication about cryostats, look under "Supplements" a
We had our Joint Commission inspection today (no deficiencies!) and the
inspector was pressing us to do temperature monitoring in a different way. For
chemicals in fridges and freezers we use the manufacturer recommendation of
temperature range to set our ranges. Typically for a fridge it is 2C
John, we love it when you "ramble!" It gives us an appreciation for the history
and breadth of histo techniques.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: John Kiernan via
Martha, that is an extreme view. Eye and face protection in pathology are
primarily for splash protection from either chemicals or body fluids. I can't
see any need for people sectioning, embedding, etc. Maybe for special stains.
Yes for pouring a lot of chemicals. And the idea that anyone
Akemi, all trash in gross room and histo goes into the biohazard waste.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Eileen Akemi Allison via Histonet
Rhonda, that is unfortunate. It seems with the shortage of histotechs in most
places that experience would be valued. Certainly you qualify since you were
certified under the rules at the time. But institutions can set their own
requirements as well which may be more strict. I wonder if your
Kate, we regularly re-embed paraffin-embedded tissue in epoxy resin for
electron microscopy. We are using very small tissue - kidney, liver, 1mm core
biopsies.
For that we run thru xylene 4 x 30 min at 60C, 100% ethanol 3 x 10 min, 95, 70,
50% ethanol 5 min each, then to distilled water, then
We try to be at 0.5% or less. We monitored as a quality indicator for several
years and had to modify something to get to that point, but it is pretty steady
now. We have new residents constantly rotating into pathology so it is a
training issue every month.
Tim Morken
Supervisor, Electron
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Morken, Timothy
Sent: Wednesday, March 27, 2019 8:30 AM
To: 'P Sicurello'; MARY ANN
Subject: RE: [Histonet] Fridge temp
You will
This from Zeus Scientific: They will now handle all IFA reagents from their
facility.
https://www.zeusscientific.com/news-events/zeus-scientific-to-offer-its-zeus-ifa-product-line-directly-to-customers-in-the-united-states
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special
Pantomics has several expression controls and has high quality products.
https://www.pantomics.com/tissue-arrays/breast
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: John
I'll note that we have used glass coverslips in histology forever but are
switching to tape not for the speed (though that is appreciated) but because
the tape dries instantly and can be put in a slide scanner right away, while
glass slides cannot until dry enough - takes a lot longer. In fact,
Does anyone know of an acid cabinet for just a few 500ml bottles? All I can
find fit 6 1-liter bottles, no smaller.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
___
If you do temp work and are looking around for the next position, we are
looking for at least a 3-month temp specifically for muscle histochem - frozen
sectioning and histochemistry. Will also do kidney FS and IF staining on the
Bond.
Or, in addition, if you know how to do EM sectioning that
Hi all, I used to get bench protector paper (paper and plastic backing) from
Cardinal that had a waffle pattern. We really like it. Now they substituted it
with a really flimsy paper surface that constantly bunches up whereas the other
type did not. Does anyone know where to get the other type
Histotechnologist I-II opening at University of California, San Francisco
Medical Center, San Francisco, CA
Job ID 17907
See posting at:
https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST=U=Applicant=1=17907=2
Tim Morken
Cleaning supplies may be stored under the sink. Bleach is a cleaning agent. We
do it and no inspector looking there has ever said anything about it.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original
Caroline, The only cameras in our lab and the histo lab are pointed at the
tissue processor screens so we can log in and see what is going on when we get
an error alert.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical
That'll be tough for our histo lab - there are no doors. It is open to two
hallways and adjacent clin lab. That said, we have so many hoods in there that
we probably make the entire floor negative pressure! And there is no odor from
the lab.
Tim Morken
Supervisor, Electron
Can anyone give me a rational for using cold (refrig or freezer-temp) acetone
to fix frozen sections? Or a rational for using RT acetone.
This is for kidney or muscle bx frozens for immmunofluroescence or
immunoperoxidase staining.
Normally they air dry for at least 15 minutes (just waiting
Charles, for grossing you do need to show competency for the types of specimens
they are allowed to gross.
Histology "competency" is not required under CLIA because it is not high
complexity (no "testing" is done. The "test" is the pathologists
interpretation). Therefore, your histology
Cassandra, the requirement for humidity covers requirements for equipment
(specifications will specify operating conditions - temperature, humidity),
chemicals (some are hygroscopic and high humidity can shorten shelf life) and
"working environment." If you find that you need certain humidity
I guess the question is, who told you this and what is their reference?
Most special stains don't require a hood. Only those that have volatile
chemicals or odors that may be offensive (ie, ammonium sulfide). We have some
areas we do specials without a hood. Our main special stain areas have a
Paula, since it is variable we strive to not have unstained slides. We had kept
them indefinitely, then when storage was overwhelming us we reduced it to 2
months maximum. Now we require request for unstained to be ordered in the
system and delivered to the pathologist. We do not hold any in
Morken, Timothy would like to recall the message, "Histotechnologist, nights,
Saturday thru Thursday, UCSF Medical Center, San Francisco, CA".
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
A position is open for an night-shift histotech at UCSF, San Francisco.
Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM
The Histology Lab at UCSF is a full service lab with routine, special stains,
IHC and ISH. The workflow is fully barcoded and all equipment
Here is the answer I got from Sigma-Aldrich Tech Support.
" We would not recommend bringing the entire bottle up to room temperature to
weigh. Repeated freeze/thaw cycles can degrade the product. If you are worried
about ice crystals affecting weight, you can bring a small sample up to room
We have a debate going on for those freezer-stored powdered chemicals used for
enzyme histochemistry.
One side says warm up the container to room temperature before opening in order
to prevent water condensation in the container/powder.
The other side says to use right away and put back in the
Jose, do you have Cerner Copath already? If so, and you plan to keep it, AB
is probably the best option. If you try to use a third-party system like
Vantage or Cerebro with Copath, Cerner will require that you buy AB anyway as
the middle-ware. Then you would need to support two separate
Fran, I've never heard of that. The books I looked at just say "filter" with no
specific filter recommendations.
For Sudan Black and Congo red we use Whatman "Reeve Angel 802" (Pleated). It
has a 15um pore size which is "coarse."
Fisher catalog number 09-901A
Tim Morken
Pathology Site
I've used hundreds of TMA's from Pantomics and Biochain with great results.
https://pantomics.com/
https://www.biochain.com/
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for
EM, or any other way ? We have a case where they wanted EM on a lens to
identify a specific bacteria. We have never done it and it would take some
work to figure it out. Also, I told them that unless there
Kind of in that same vein, but not to denigrate the book ( I have not seen it),
I try to impress on people that "enabling" a poor process via "workarounds" or
"shortcuts" does nothing to solve the original problem - it just prolongs the
problem and peoples frustration with the problem. A much
Sandra, yes, we use DI water from a water system in our lab
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Sandra Cheasty via Histonet
Martha, The question to ask your EHS is how much extra exposure they will allow
employees. Recycling removes all the salts so you need to add them back. Then
it is just like making up formalin from scratch. Does anyone do that anymore?
You need a minimum of full-face vapor masks to prevent
We don't but there are decibel monitor phone apps. Not sure how accurate they
are...
I've used one called Sound Meter. It shows 70+ db next to our -80 freezer and
90+ db next to the 6-foot fume hood.
In our lab with cryostats it shows 60+ and in a quiet lab, no equipment
running, it shows 50+
things about AP Beaker.
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard \ Winston-Salem, NC 27157
p 336.716.2109 \ f 336.716.5890
mw...@wakehealth.edu<mailto:mw...@wakehealth.edu>
-Original Message-
From: Morken, Timothy via Histon
I am interested as well. "They" are threatening us with a move to Beaker in the
future
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Blake
We use Permaslip acrylic for manual coverslipping. Sakura media for our
automated coverslipper. We have also used Richard-Allan CytoSeal with great
resuls. We stopped using one called Quick-mount (comes in tube) because they
had a batch that was bad and the media turned cloudy and the slips
Charles, we use General Data labels on the Cognitive printers (not sure if that
is the printer you are using). We were using their 22 x 22 StainerShield for
the Leica Bond as well as all histo labels. Then we switched to their
StainerShield 24 x 22 "Ventana-style" label and use them on both
Haley,
Maybe ask Stephen directly. He is on histonet, and has a website:
https://www.pathologyinnovations.com/
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original
Renee, for practical purposes in histology they are pretty much the same. Note
that the original water quality is key because certain things can carry over in
each type of system. Commercial suppliers do all the filtering, conditioning
and finishing for you.
Deionized (DI) is now the usual
Elaine, blocks last a very long time. The at the face the tissue may be
oxidized, like a section on a slide, but the deeper tissue should be good. If
you have several extra blocks just coat the face with paraffin to protect it.
However, the only way to be sure is to test them occasionally. If
Hi, does anyone know a source for the charcoal filters for the old Lerner
Laboratories Fume-gard hood, model 912, filter #906?
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Hi all, I am interesting anyone's experience EM embedding of long renal cores,
say in 5mm increments, rather that dividing to 1 to 2 mm pieces, for blocks,
and thick sections.
We are wondering about then going on to produce EM thin sections of gloms
identified for EM. Is the core re-trimmed
Emily, ? Are you ensuring excess water is not under the section that would
prevent adherence to the slide? Are you using slides recommended by the outside
lab? Do they recommend a procedure for drying the slides before sending?
Just a starting point...
Tim Morken
Pathology Site Manager,
Luca, sounds like a project to figure that out!
Anytime you soak the tissue in any percentage alcoholic solution there is going
to be some dehydration - substitution of water with alcohol.
When adding alcohol you will cause precipitation of some of the proteins in the
tissue - depends on the
Ana, you should fix before sectioning. Chris Van der Loos did an excellent
study on loss of cytokines in frozen sections vs whole cells. See his paper:
Immunohistochemical Detection of Interferon-y, Fake or Fact? CM Van der Loos,
et.al., J Histochem Cytochem, 49(6):699-709, 2001. Www.jhc.org
Really? You want to compare your lab to Amazon? If you have stock on-hand,
located in one place, have robots to retrieve it, people who do nothing but
pack boxes and trucks standing by to take any order at any time of day, then
you are in the league of Amazon and can receive an order and send
We have a PA Supervisor position open at UCSF Medical Center. Oversees 3
grossing sites (3 hosptitals) and morgue/autopsy, trains residents.
Oversees 9 PA's, 4 accessioners, administrative staff.
Apply online at: http://jobs.ucsfmedicalcenter.org/
Job Description
Pathologists' Assistants
Jason,
The only way is to validate for those conditions. You would need some
specimens, including known controls, handled and processed in those same
conditions and compare to samples processed under the acceptable conditions.
You can do it, it will just take some time.
Tim Morken
Bob, Thanks for the offer! I finally found a copy and Google Translate did a
pretty good job on it, and we have a person in the lab who, it turns out, can
read german, so I think we are set.
As an aside, the "clues" I got from tidbits of the procedure mentioned in other
papers turned out to
1 - 100 of 159 matches
Mail list logo