Re: [Histonet] Retirement in sight!

2021-09-09 Thread Morken, Timothy via Histonet
ave to say it was prominent in our careers. It was where I was first exposed to Histology. I have enjoyed your friendship and comradeship. Take care Victor Sent from my iPad > On Sep 9, 2021, at 9:26 AM, Morken, Timothy via Histonet > wrote: > >  > After 40 years in the lab I've de

[Histonet] Retirement in sight!

2021-09-09 Thread Morken, Timothy via Histonet
After 40 years in the lab I've decided to retire this year - in a week actually! It has been an interesting 4 decades... I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at

[Histonet] Copy of an old EM resin paper?

2021-08-25 Thread Morken, Timothy via Histonet
Can anyone supply me a copy of this paper on epoxy resins? I can't find it anywhere on line and our library does not have that journal. Glauert AM: Epoxy Resins: An update on their selection and use. Microscopy and Analysis 15-20 Sept 1991. Tim Morken Supervisor, Electron

Re: [Histonet] cryostat

2021-08-09 Thread Morken, Timothy via Histonet
Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two Epredia NX70 cryostats. We've used them for 8 years now and really like them. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

[Histonet] EM immunolabeling for kappa, lambda light chains in amyloid?

2021-07-29 Thread Morken, Timothy via Histonet
Can anyone give me any tips on EM immuno-gold labeling for kappa and lambda light chains in amyloid cases? We use Eponate12 for embedding. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

Re: [Histonet] Specimen tracking scenario, accountability

2021-06-22 Thread Morken, Timothy via Histonet
Curt, You can make and use barcodes in MS Excel or Access and print labels. If you keep the clients items together in some kind of bag or container you can put the label on the outside and scan at each station to track them. You just need to be sure the items are all kept together to ensure

Re: [Histonet] Problems with DIF staining of C4d

2021-06-08 Thread Morken, Timothy via Histonet
Beth, which C4d are you using? And it is a FITC-labeled primary? We use it as a two-step rather than FITC-labeled primary. That increases the sensitivity significantly. Gloms have C4d so act as an internal control. We use C4d antibody, 100ul vial, unlabeled primary. Diluted 1:200 in Dako

Re: [Histonet] IF with permanent mounting media?

2021-05-28 Thread Morken, Timothy via Histonet
London, Canada = = = From: Morken, Timothy via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: May 28, 2021 11:31 AM To: Histonet mailto:histonet@lists.utsouthwestern.edu>> Subject: [Histonet] IF with permanent mounting media? Has anyone tried usin

Re: [Histonet] IF with permanent mounting media?

2021-05-28 Thread Morken, Timothy via Histonet
months. I realized I did not reply to the histonet the last time, I still need to get myself familiarized with this : ( John Lai Posdoctoral Fellow, School of Life Science, HKUST -Original Message- From: Morken, Timothy via Histonet Sent: Friday, May 28, 2021 11:31 PM To: Histonet

[Histonet] IF with permanent mounting media?

2021-05-28 Thread Morken, Timothy via Histonet
Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC

Re: [Histonet] Cryostat

2021-05-19 Thread Morken, Timothy via Histonet
-Original Message- From: Morken, Timothy via Histonet Sent: Wednesday, May 19, 2021 8:26 AM To: Brittany Hethcox Cc: Histonet Subject: Re: [Histonet] Cryostat Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours

Re: [Histonet] Cryostat

2021-05-19 Thread Morken, Timothy via Histonet
Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours and it makes a difference for different size people in the lab. It also can have a vapor disinfectant system which we use and it works great. We use ours for

Re: [Histonet] Automated Coverslippers

2021-05-13 Thread Morken, Timothy via Histonet
Jennifer, we have used the Sakura Glas, Glas2 (attached to prisma stainer), and now the Sakura tape cover slipper attached to the Prisma. Also we have had the Leica CV5030 glass coverslipper. All worked as long as they are kept clean. Daily maintenance is a must. If in a service contract they

Re: [Histonet] JCAHO labs

2021-05-10 Thread Morken, Timothy via Histonet
Jessica, CAP and JC apply the same CLIA regulations, just in a different manner. CAP tends to apply more of their own "upgrades." Ie, makes it a bit stricter, which is allowed. CLIA is just the baseline. If you have been thru CAP inspections you will not have any problem with JC. While CAP

Re: [Histonet] Antibody diluent

2021-03-23 Thread Morken, Timothy via Histonet
Maria, we went back to using Dako Diluent, which we had just switched away from to save money! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Maria Cruz via Histonet Sent:

[Histonet] IHC Disclaimer for immunofluorescence stains

2021-02-12 Thread Morken, Timothy via Histonet
Those labs doing diagnostic immunofluorescence for kidney or skin, do you put in the IHC disclaimer used for peroxidase staining on the reports? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

[Histonet] Other Histonet-like listservers?

2021-02-11 Thread Morken, Timothy via Histonet
Hi all, I'm going to give a presentation on online histology help sites like Histonet and the NSH Block. Does anyone know of other listservers that can be of help to histotechnologists in all fields? I checked the MSA EM listserver but it is down for some technical reason. Thanks in advance

[Histonet] Milestone Prestochill for muscle histochem

2021-01-29 Thread Morken, Timothy via Histonet
Hi all, I was wondering if anyone has used the Milestone PrestoChill for freezing muscle for histochemistry. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ___ Histonet

Re: [Histonet] Order Test in Histology

2021-01-22 Thread Morken, Timothy via Histonet
Amy, all our orders are thru our LIS, Copath Plus. Residents or pathologist order on their own. Except for grossed cases. They get standard panels ordered upon accession or grossing. Outside cases are all consults. Some are routine (for instance our kidney lab where we do routine panels) so

[Histonet] Pre-made chrome alum slides source?

2021-01-08 Thread Morken, Timothy via Histonet
Our source for pre-made chrome alum-subbed slides does not exist anymore. Does anyone know of a company that produces these? (American Master Tech had them but when StatLab bought them they discontinued the chrome alum slides) We used these for EM thick sections because they hold a water drop

[Histonet] FW: Pay range for histology supervisor

2021-01-08 Thread Morken, Timothy via Histonet
I’m in central PA Sent from my iPhone > On Jan 8, 2021, at 3:03 PM, Morken, Timothy wrote: > > Dee, it depends on where you are > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > -Original

Re: [Histonet] Pay range for histology supervisor

2021-01-08 Thread Morken, Timothy via Histonet
Dee, it depends on where you are Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Dee Neamand via Histonet Sent: Friday, January 08, 2021 11:47 AM To:

Re: [Histonet] Joint Commission Standards for Pathology

2021-01-07 Thread Morken, Timothy via Histonet
Amy, they don't have a specific AP and General checklist like CAP has. They have a Standards book that lists all the requirements and you need to go thru and figure out which apply to your area. It is also on line at the TJC site. The thing is that TJC covers the entire hospital, not just the

Re: [Histonet] Question about accessioning outside consult cases

2020-12-10 Thread Morken, Timothy via Histonet
Martha, we give it the same number sequence as any other surgical or cytology case, but we can identify our cases by "Specimen Class" so consults get a specimen class according to the type of consult - cyto, cytogyn, surgical, etc. The specimen class is printed on the labels for all

Re: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks

2020-12-08 Thread Morken, Timothy via Histonet
Kelly, You are not going to stay within the temp ranges if putting pellets directly into the embedding center tank. A couple solutions: 1) write into your procedure that the temperature will go down when pellets are added and that once melted should be in whatever range it calls for (and don't

Re: [Histonet] DIF in paraffin section

2020-11-02 Thread Morken, Timothy via Histonet
Manahil, we do IF on paraffin sections on occasion when kidney tissue is insufficient (and then only IgA, G, M and K, L) or they want the markers on tissue that is only embedded in paraffin, such as kappa, Lambda for amyloid. We use at a higher concentration than for frozens (1:10 vs 1:100)

[Histonet] FW: Temperature Checks

2020-10-07 Thread Morken, Timothy via Histonet
Amy, we have been using Awarepoint, but they are apparently going out of business so we are switching over to Stanley Aeroscout on all fridges, freezers and room temp/humidity throughout our facilities. We have direct access to the monitoring of each "asset" and can check it online at any

Re: [Histonet] frozen section/histo duties

2020-08-26 Thread Morken, Timothy via Histonet
Nancy, we have HT (Histotechs) and HLT (Hospital lab techs - lab assistants) who work in grossing and do the frozen sections with residents and pathologists. There is no requirement that a person cutting sections has to be certified in anything. Our HLT's generally do all the accessioning but

Re: [Histonet] frozen section/histo duties

2020-08-26 Thread Morken, Timothy via Histonet
" The way I read it, the only things in pathology that are actually high complexity testing that aren't performed exclusively by pathologists are and QCing special stain/ISH/IHC slides (ANP.21395). " CLIA regulations specifically state that quality control is not a test so it is not

Re: [Histonet] slide printer

2020-08-25 Thread Morken, Timothy via Histonet
When we implemented barcoding I was planning on using direct slide printing. It seemed the most efficient and safest way (ie, no mislabelling) to do the labeling. However, when we looked into getting the printers they were on the order of $9,000 per printer. And we wanted 20 printers for

Re: [Histonet] Blank Spots on IHC- Bubbles on Bond

2020-08-14 Thread Morken, Timothy via Histonet
Greg, thanks! I have a leica engineer coming in to day to look at it, specifically the fluidics system, so hopefully he’ll see what you mention and fix it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From:

Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

2020-08-13 Thread Morken, Timothy via Histonet
g a surfactant. Extreme solution: place solutions in a vacuum. Sent from Smallbiz Yahoo Mail for iPhone On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet mailto:histonet@lists.utsouthwestern.edu>> wrote: Has anyone solved a problem on the Leica Bond stainer of bubbles ov

[Histonet] Blank spots on IHC tissues - bubbles on Bond

2020-08-12 Thread Morken, Timothy via Histonet
Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides

[Histonet] slide scanning and glass vs plastic RE: Histonet Digest, Vol 201,

2020-08-06 Thread Morken, Timothy via Histonet
Steve, I agree with your whole thing. And our pathologist fought against plastic for years simply due to the fact it scratches easily and we are constantly pulling and filing slides for various needs. However, when we decided to go to scanning slides wet mounting media was never going to work -

Re: [Histonet] Update info. on old topic?

2020-08-05 Thread Morken, Timothy via Histonet
Hi Jeanine! We are using film coverslipping exclusively now and scanning all slides. We moved to film because it dries almost instantly and can be scanned right away. It has worked very well. The only issues, and not specific to film coverslipping, are that some slides with low contrast

Re: [Histonet] Apple Green Birefringence in Amyliod slides

2020-07-28 Thread Morken, Timothy via Histonet
I neglected to mention sections should be cut at 10um for best results. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Morken, Timothy via Histonet Sent: Tuesday, July 28, 2020

Re: [Histonet] Apple Green Birefringence in Amyliod slides

2020-07-28 Thread Morken, Timothy via Histonet
Ken, Yes, polarized light and apple green birefringence is diagnostic for amyloid with congo red and is the best practice. If you have a problem with known control slides there are two possibilities: 1) make up fresh solution. The pH has to be right. Or 2) try other control slides. Maybe you

Re: [Histonet] Lab assistant/histotech

2020-07-06 Thread Morken, Timothy via Histonet
Samantha, Yes there is: Staffing Benchmarks for Histology Laboratories Rene Buesa Annals of Diagnostic Pathology Volume 14, Issue 3, June 2010, Pages 182-193 He details here how lab assistants make labs more productive by allowing trained histotechs to do the embedding, sectioning and staining

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Morken, Timothy via Histonet
Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections. Tim Morken Supervisor, Electron Microscopy/Neuromuscular

[Histonet] Who works in Hospitals?

2020-04-14 Thread Morken, Timothy via Histonet
Something for others to know about https://www.linkedin.com/posts/activity-6653762952572788737-jqh8 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ___ Histonet mailing

[Histonet] EM as a "contract lab" lab for clia purposes

2020-03-30 Thread Morken, Timothy via Histonet
Here's a question that came up. A stand-alone EM lab at a college that is associated with a medical center, but in a different city, wants to do the EM workup for the medical center. But they would only take in samples, do the EM processing and take images then the pathologist would read the

[Histonet] grossing manuals

2020-01-22 Thread Morken, Timothy via Histonet
Posted for a friend... To my knowledge the best institutional manual is the University of Michigan's Cutting Manual. -Izak Dimenstein ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Pregnant in histo lab. Am I safe?

2020-01-21 Thread Morken, Timothy via Histonet
The problem with xylene is that the acceptable air level in the lab is 100ppm but humans can detect it by smell at the 5 - 20ppm range. So it seems like it is "everywhere" but it could still be at a very low level. What level is safe for a pregnancy? CDC has some info on this:

[Histonet] Recall: SECURE: Please send block to EM lab

2019-12-11 Thread Morken, Timothy via Histonet
This is a secure message. Click here https://pe-002e8f01.gslb.pphosted.com/formpostdir/securereader?id=1VTCLvZhK2OwIXUVqkgYRCeLHWKdc-FL=66d9f965 by 2019-12-18 09:14 PST to read your message. After that, open the attachment. [Image?c=logo=1=2=3.1510941425865]

[Histonet] SECURE: Please send block to EM lab

2019-12-11 Thread Morken, Timothy via Histonet
This is a secure message. Click here https://pe-002e8f01.gslb.pphosted.com/formpostdir/securereader?id=TSHBjjhKZJQbAmmmM9ouoE388nfx0p00=66d9f965 by 2019-12-18 09:03 PST to read your message. After that, open the attachment. [Image?c=logo=1=2=3.1510941425865]

Re: [Histonet] contaminating patient requesition forms

2019-11-12 Thread Morken, Timothy via Histonet
Paula, we use Cerner Copath Plus and scan into the case all requisitions and any other paperwork at accessioning. The person grossing uses the barcode on hard copy requisition to access the case but the hard copy stays in the gross room so no contaminated paperwork (or any paperwork at all)

[Histonet] Muscle histochemistry workflow?

2019-11-07 Thread Morken, Timothy via Histonet
I'm wondering how other muscle histochem labs handle their workflow. Because the histochem stains require frozen sections and a freshly-prepared reagents, and muscle conditions are not usually very time sensitive, we batch all our cutting and staining to one batch per week. We have 5-10 cases a

Re: [Histonet] Freezing Sprays in Cryostats

2019-09-26 Thread Morken, Timothy via Histonet
I wonder if it is just a coincidence that and ad for this device from Milestone came thru in the middle of this freezing spray string https://www.milestonemedsrl.com/us/product/prestochill/ Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology

[Histonet] Reference website RE: Flammable Sprays in Cryostats

2019-09-26 Thread Morken, Timothy via Histonet
You can see past issues of Morbidly and Mortality Weekly Report (MMWR) at: https://www.cdc.gov/mmwr/index2019.html For past issues, click on "weekly report" on the left hand side and you can get to a list of past issues. However, for the publication about cryostats, look under "Supplements" a

[Histonet] A little temperture monitoring debate

2019-09-11 Thread Morken, Timothy via Histonet
We had our Joint Commission inspection today (no deficiencies!) and the inspector was pressing us to do temperature monitoring in a different way. For chemicals in fridges and freezers we use the manufacturer recommendation of temperature range to set our ranges. Typically for a fridge it is 2C

Re: [Histonet] Mallory-Azan stain

2019-09-10 Thread Morken, Timothy via Histonet
John, we love it when you "ramble!" It gives us an appreciation for the history and breadth of histo techniques. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: John Kiernan via

Re: [Histonet] PPE for the Histology lab

2019-09-09 Thread Morken, Timothy via Histonet
Martha, that is an extreme view. Eye and face protection in pathology are primarily for splash protection from either chemicals or body fluids. I can't see any need for people sectioning, embedding, etc. Maybe for special stains. Yes for pouring a lot of chemicals. And the idea that anyone

Re: [Histonet] Disposal of contaminated gloves, paper towels etc... after grossing

2019-08-29 Thread Morken, Timothy via Histonet
Akemi, all trash in gross room and histo goes into the biohazard waste. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Eileen Akemi Allison via Histonet

Re: [Histonet] (no subject)

2019-08-15 Thread Morken, Timothy via Histonet
Rhonda, that is unfortunate. It seems with the shortage of histotechs in most places that experience would be valued. Certainly you qualify since you were certified under the rules at the time. But institutions can set their own requirements as well which may be more strict. I wonder if your

Re: [Histonet] Re-embed agar/FFPE tissue in plastic?

2019-05-29 Thread Morken, Timothy via Histonet
Kate, we regularly re-embed paraffin-embedded tissue in epoxy resin for electron microscopy. We are using very small tissue - kidney, liver, 1mm core biopsies. For that we run thru xylene 4 x 30 min at 60C, 100% ethanol 3 x 10 min, 95, 70, 50% ethanol 5 min each, then to distilled water, then

Re: [Histonet] Benchmarks for Reprocessing

2019-05-08 Thread Morken, Timothy via Histonet
We try to be at 0.5% or less. We monitored as a quality indicator for several years and had to modify something to get to that point, but it is pretty steady now. We have new residents constantly rotating into pathology so it is a training issue every month. Tim Morken Supervisor, Electron

[Histonet] FW: Fridge temp

2019-03-27 Thread Morken, Timothy via Histonet
Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Morken, Timothy Sent: Wednesday, March 27, 2019 8:30 AM To: 'P Sicurello'; MARY ANN Subject: RE: [Histonet] Fridge temp You will

Re: [Histonet] Looking for vendor for Zeus Wash solution

2019-03-22 Thread Morken, Timothy via Histonet
This from Zeus Scientific: They will now handle all IFA reagents from their facility. https://www.zeusscientific.com/news-events/zeus-scientific-to-offer-its-zeus-ifa-product-line-directly-to-customers-in-the-united-states Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special

Re: [Histonet] ER/PR/Her2 Controls

2019-03-21 Thread Morken, Timothy via Histonet
Pantomics has several expression controls and has high quality products. https://www.pantomics.com/tissue-arrays/breast Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: John

[Histonet] tape for digital scanning...RE: Stainer vs. Stainer

2019-03-14 Thread Morken, Timothy via Histonet
I'll note that we have used glass coverslips in histology forever but are switching to tape not for the speed (though that is appreciated) but because the tape dries instantly and can be put in a slide scanner right away, while glass slides cannot until dry enough - takes a lot longer. In fact,

[Histonet] Really Small acid cabinet?

2019-03-07 Thread Morken, Timothy via Histonet
Does anyone know of an acid cabinet for just a few 500ml bottles? All I can find fit 6 1-liter bottles, no smaller. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ___

[Histonet] Temp for muscle FS and histochem, possibly EM.

2019-02-26 Thread Morken, Timothy via Histonet
If you do temp work and are looking around for the next position, we are looking for at least a 3-month temp specifically for muscle histochem - frozen sectioning and histochemistry. Will also do kidney FS and IF staining on the Bond. Or, in addition, if you know how to do EM sectioning that

[Histonet] Waffle-pattern bench paper?

2019-02-26 Thread Morken, Timothy via Histonet
Hi all, I used to get bench protector paper (paper and plastic backing) from Cardinal that had a waffle pattern. We really like it. Now they substituted it with a really flimsy paper surface that constantly bunches up whereas the other type did not. Does anyone know where to get the other type

[Histonet] Histotechnologist I-II opening at UC San Francisco

2019-02-15 Thread Morken, Timothy via Histonet
Histotechnologist I-II opening at University of California, San Francisco Medical Center, San Francisco, CA Job ID 17907 See posting at: https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST=U=Applicant=1=17907=2 Tim Morken

Re: [Histonet] Bleach Regs

2019-01-31 Thread Morken, Timothy via Histonet
Cleaning supplies may be stored under the sink. Bleach is a cleaning agent. We do it and no inspector looking there has ever said anything about it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original

Re: [Histonet] Cameras in the Laboratory

2019-01-22 Thread Morken, Timothy via Histonet
Caroline, The only cameras in our lab and the histo lab are pointed at the tissue processor screens so we can log in and see what is going on when we get an error alert. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical

Re: [Histonet] Closed doors in Histology

2018-11-16 Thread Morken, Timothy via Histonet
That'll be tough for our histo lab - there are no doors. It is open to two hallways and adjacent clin lab. That said, we have so many hoods in there that we probably make the entire floor negative pressure! And there is no odor from the lab. Tim Morken Supervisor, Electron

[Histonet] Frozen sections and cold acetone...

2018-10-25 Thread Morken, Timothy via Histonet
Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone. This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining. Normally they air dry for at least 15 minutes (just waiting

Re: [Histonet] Competency signoff CAP

2018-10-08 Thread Morken, Timothy via Histonet
Charles, for grossing you do need to show competency for the types of specimens they are allowed to gross. Histology "competency" is not required under CLIA because it is not high complexity (no "testing" is done. The "test" is the pathologists interpretation). Therefore, your histology

Re: [Histonet] histology lab temperature and humidity

2018-09-18 Thread Morken, Timothy via Histonet
Cassandra, the requirement for humidity covers requirements for equipment (specifications will specify operating conditions - temperature, humidity), chemicals (some are hygroscopic and high humidity can shorten shelf life) and "working environment." If you find that you need certain humidity

Re: [Histonet] Special stains and hoods

2018-08-22 Thread Morken, Timothy via Histonet
I guess the question is, who told you this and what is their reference? Most special stains don't require a hood. Only those that have volatile chemicals or odors that may be offensive (ie, ammonium sulfide). We have some areas we do specials without a hood. Our main special stain areas have a

Re: [Histonet] Unstained slides - how long are they good for?

2018-08-17 Thread Morken, Timothy via Histonet
Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in

[Histonet] Recall: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA

2018-08-16 Thread Morken, Timothy via Histonet
Morken, Timothy would like to recall the message, "Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA". ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA

2018-08-16 Thread Morken, Timothy via Histonet
A position is open for an night-shift histotech at UCSF, San Francisco. Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM The Histology Lab at UCSF is a full service lab with routine, special stains, IHC and ISH. The workflow is fully barcoded and all equipment

Re: [Histonet] Warm up freezer-stored powder, or use right away?

2018-06-01 Thread Morken, Timothy via Histonet
Here is the answer I got from Sigma-Aldrich Tech Support. " We would not recommend bringing the entire bottle up to room temperature to weigh. Repeated freeze/thaw cycles can degrade the product. If you are worried about ice crystals affecting weight, you can bring a small sample up to room

[Histonet] Warm up freezer-stored powder, or use right away?

2018-05-24 Thread Morken, Timothy via Histonet
We have a debate going on for those freezer-stored powdered chemicals used for enzyme histochemistry. One side says warm up the container to room temperature before opening in order to prevent water condensation in the container/powder. The other side says to use right away and put back in the

[Histonet] FW: Cerner AB

2018-04-25 Thread Morken, Timothy via Histonet
Jose, do you have Cerner Copath already? If so, and you plan to keep it, AB is probably the best option. If you try to use a third-party system like Vantage or Cerebro with Copath, Cerner will require that you buy AB anyway as the middle-ware. Then you would need to support two separate

Re: [Histonet] Congo Red- Glass wool

2018-03-26 Thread Morken, Timothy via Histonet
Fran, I've never heard of that. The books I looked at just say "filter" with no specific filter recommendations. For Sudan Black and Congo red we use Whatman "Reeve Angel 802" (Pleated). It has a 15um pore size which is "coarse." Fisher catalog number 09-901A Tim Morken Pathology Site

Re: [Histonet] IHC validation

2018-03-22 Thread Morken, Timothy via Histonet
I've used hundreds of TMA's from Pantomics and Biochain with great results. https://pantomics.com/ https://www.biochain.com/ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

[Histonet] Prosthetic lens processing, cutting?

2018-03-08 Thread Morken, Timothy via Histonet
Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there

Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

2018-01-16 Thread Morken, Timothy via Histonet
Kind of in that same vein, but not to denigrate the book ( I have not seen it), I try to impress on people that "enabling" a poor process via "workarounds" or "shortcuts" does nothing to solve the original problem - it just prolongs the problem and peoples frustration with the problem. A much

Re: [Histonet] Hard Water and Auto Stainers

2017-12-20 Thread Morken, Timothy via Histonet
Sandra, yes, we use DI water from a water system in our lab Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Sandra Cheasty via Histonet

[Histonet] FW: Recycling formalin

2017-12-20 Thread Morken, Timothy via Histonet
Martha, The question to ask your EHS is how much extra exposure they will allow employees. Recycling removes all the salts so you need to add them back. Then it is just like making up formalin from scratch. Does anyone do that anymore? You need a minimum of full-face vapor masks to prevent

Re: [Histonet] Noise Levels in Lab

2017-12-05 Thread Morken, Timothy via Histonet
We don't but there are decibel monitor phone apps. Not sure how accurate they are... I've used one called Sound Meter. It shows 70+ db next to our -80 freezer and 90+ db next to the 6-foot fume hood. In our lab with cryostats it shows 60+ and in a quiet lab, no equipment running, it shows 50+

Re: [Histonet] Beaker with or without Vantage

2017-11-29 Thread Morken, Timothy via Histonet
things about AP Beaker. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu<mailto:mw...@wakehealth.edu> -Original Message- From: Morken, Timothy via Histon

Re: [Histonet] Beaker with or without Vantage

2017-11-28 Thread Morken, Timothy via Histonet
I am interested as well. "They" are threatening us with a move to Beaker in the future Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Blake

Re: [Histonet] mounting medium

2017-11-13 Thread Morken, Timothy via Histonet
We use Permaslip acrylic for manual coverslipping. Sakura media for our automated coverslipper. We have also used Richard-Allan CytoSeal with great resuls. We stopped using one called Quick-mount (comes in tube) because they had a batch that was bad and the media turned cloudy and the slips

Re: [Histonet] Inventory purchasing question

2017-10-10 Thread Morken, Timothy via Histonet
Charles, we use General Data labels on the Cognitive printers (not sure if that is the printer you are using). We were using their 22 x 22 StainerShield for the Leica Bond as well as all histo labels. Then we switched to their StainerShield 24 x 22 "Ventana-style" label and use them on both

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

2017-10-06 Thread Morken, Timothy via Histonet
Haley, Maybe ask Stephen directly. He is on histonet, and has a website: https://www.pathologyinnovations.com/ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original

Re: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water

2017-10-05 Thread Morken, Timothy via Histonet
Renee, for practical purposes in histology they are pretty much the same. Note that the original water quality is key because certain things can carry over in each type of system. Commercial suppliers do all the filtering, conditioning and finishing for you. Deionized (DI) is now the usual

Re: [Histonet] Old control blocks

2017-10-05 Thread Morken, Timothy via Histonet
Elaine, blocks last a very long time. The at the face the tissue may be oxidized, like a section on a slide, but the deeper tissue should be good. If you have several extra blocks just coat the face with paraffin to protect it. However, the only way to be sure is to test them occasionally. If

[Histonet] Replacement filters for Lerner Fume-Gard benchtop hood?

2017-09-19 Thread Morken, Timothy via Histonet
Hi, does anyone know a source for the charcoal filters for the old Lerner Laboratories Fume-gard hood, model 912, filter #906? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

[Histonet] EM renal embedding of long cores

2017-09-08 Thread Morken, Timothy via Histonet
Hi all, I am interesting anyone's experience EM embedding of long renal cores, say in 5mm increments, rather that dividing to 1 to 2 mm pieces, for blocks, and thick sections. We are wondering about then going on to produce EM thin sections of gloms identified for EM. Is the core re-trimmed

Re: [Histonet] IHC

2017-08-10 Thread Morken, Timothy via Histonet
Emily, ? Are you ensuring excess water is not under the section that would prevent adherence to the slide? Are you using slides recommended by the outside lab? Do they recommend a procedure for drying the slides before sending? Just a starting point... Tim Morken Pathology Site Manager,

Re: [Histonet] Fresh tissue soaked in ethanol solution prior to formalin fixation

2017-07-10 Thread Morken, Timothy via Histonet
Luca, sounds like a project to figure that out! Anytime you soak the tissue in any percentage alcoholic solution there is going to be some dehydration - substitution of water with alcohol. When adding alcohol you will cause precipitation of some of the proteins in the tissue - depends on the

Re: [Histonet] IHC for secreted proteins and cytokines in frozen sections - Pre-fix or post-fix?

2017-06-02 Thread Morken, Timothy via Histonet
Ana, you should fix before sectioning. Chris Van der Loos did an excellent study on loss of cytokines in frozen sections vs whole cells. See his paper: Immunohistochemical Detection of Interferon-y, Fake or Fact? CM Van der Loos, et.al., J Histochem Cytochem, 49(6):699-709, 2001. Www.jhc.org

Re: [Histonet] Send-Outs

2017-05-16 Thread Morken, Timothy via Histonet
Really? You want to compare your lab to Amazon? If you have stock on-hand, located in one place, have robots to retrieve it, people who do nothing but pack boxes and trucks standing by to take any order at any time of day, then you are in the league of Amazon and can receive an order and send

[Histonet] FW: PA Supervisor Position, San Francisco

2017-05-11 Thread Morken, Timothy via Histonet
We have a PA Supervisor position open at UCSF Medical Center. Oversees 3 grossing sites (3 hosptitals) and morgue/autopsy, trains residents. Oversees 9 PA's, 4 accessioners, administrative staff. Apply online at: http://jobs.ucsfmedicalcenter.org/ Job Description Pathologists' Assistants

Re: [Histonet] Breast Her2Neu IHC vs FISH

2017-04-19 Thread Morken, Timothy via Histonet
Jason, The only way is to validate for those conditions. You would need some specimens, including known controls, handled and processed in those same conditions and compare to samples processed under the acceptable conditions. You can do it, it will just take some time. Tim Morken

Re: [Histonet] Hirsch-Pfeiffer cresyl violet method

2017-04-17 Thread Morken, Timothy via Histonet
Bob, Thanks for the offer! I finally found a copy and Google Translate did a pretty good job on it, and we have a person in the lab who, it turns out, can read german, so I think we are set. As an aside, the "clues" I got from tidbits of the procedure mentioned in other papers turned out to

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