[Histonet] AFB Control Question

[Marcum, Pamela A via Histonet]

Good morning,

We are attempting to find AFB controls from human sources.  We would trade if 
we have something you need and we have available.  Does anyone have blocks they 
might share or trade without upsetting our HIPPA people on either side.  I am 
really not interested in buying slides as we cut larger blocks down and have 
controls on with the patient tissue.  Also the control slides are general in 
the middle of the slide and force to use two slides for every test (too many 
ordering and all want a control) which makes the cost prohibitive.  Our 
pathologists prefer human tissue and most of the companies I talk to either 
don't know or won't say it is animal origin or human.


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[Histonet] Small Slide Dryer or Oven

[Marcum, Pamela A via Histonet]

We are looking for a small slide dryer (hold up to three 30 position short 
racks) that will reach 65C and when opened return to temperature within 1 or 2 
minutes.  It does not have to fancy and is only used for special stains so it 
is not a constant use item.

Pam

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[Histonet] ATRX Antibody

[Marcum, Pamela A via Histonet]

I am asking this question for our IHC Supervisor.  She is looking for a source 
for the ATRX antibody as either an RUO or preferably IVD.  They use the Ventana 
Ultras and Benchmarks so if anyone has a procedure that would be great.

Pam

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Re: [Histonet] PAS/Decal Question

[Marcum, Pamela A via Histonet]

We have no say in the decal procedure and I have been fighting this for 6 
years.  It is determined by our HemePath docs and when they want bone marrows 
completed for clinics the next day.  We have a 24 hour turnaround time, which 
is ridiculous however; we cannot not change it.  

The feedback we get now is the PAS stain on the bone marrows Levels 2 and 4 are 
not staining evenly.  They show a smudginess in some cells on the lower or 
level 4 section/ or they look over processed/cooked.  WE have done manual, 
instrument and now Dako Artisan (Dako has bend over backward to help) and 
nothing changes what they see on the slides.  They will not even consider 
changing the early steps as I have been told decal is fine (no its not) and 
processing is fine.  Funny thing H are always fine and only the PAS is a 
problem.  Just tired of it.  

This has been a losing fight for almost 5 months and I reached out to all of 
you in hopes it would help us make some changes.  Thank you for your 
suggestions unfortunately no one wants to hear about changes if they mean it is 
not a 24 hour turnaround time.  I am almost ready to just say "garbage in 
garbage out".  I feel very bad for the patients at this point.  I have not 
given up just trying to find a way to get change and better results.  

Thank You All for Your Suggestions!! 

Pam 

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Thursday, May 05, 2016 2:19 AM
To: Marcum, Pamela A <pamar...@uams.edu>
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] PAS/Decal Question

Hi Pam,
my personal opinion is, that 2 hours fixation is too short for sufficient 
tissue-protection before acid decalcification. Formic acid at 50°C must have an 
impact on glycoproteins. Wether it is a kind of solving the "sugars" or 
beginning oxidation of the OH-groups (like periodic acid does in the 
PAS-reaction).

In our lab we do also acid decal with formic acid for at least 6 hours at RT, 
after one day in NBF. Our processing protocol is the routine-protocol over 
night. How thick are your BMT, also 3-4 mm?
In my opinion 4 hours are a challenge. Are the other stainings of the BMT 
optimal or show sometimes similar outcome? "Smudginess" reminds me of 
insufficient infiltration.

I also see that our PAS is not as bright as in the other specimens without 
decal. Sometimes it gives more the impression of a diastase-PAS. 

Gudrun

-----Ursprüngliche Nachricht-
Von: Marcum, Pamela A via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Dienstag, 03. Mai 2016 18:39
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] PAS/Decal Question

We are still having issues with our PAS stain on decaled bone marrows.  The 
Pathologists in HemePath are seeing what they refer to as smudginess in cells 
on some areas of the completed PAS slides.  We have looked at everything and 
cannot find where the issue is coming from at this point.  We have done manual 
staining for PAS, automated on the Leica stainer and on the Dako Artisan.  All 
methods show the same result for some slides.  We can go for several days to a 
week or more with no problem and then suddenly it is back and we have changed 
nothing in the way we do the processing, embedding, sectioning, 
deparaffinization and coverslipping.  We do as many as 38 bone marrow cores a 
night or as few as 8 and can find no correlation in the number we have to deal 
with for a given period.  All bone marrows drawn today must be completed by 8AM 
tomorrow morning.

Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a 
maximum of 7 hours +/-.

Grossed and placed in cassettes for 15 minute rinse in running DI Water

Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C

Rinsed in running DI water for 15 minutes

Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours 
minimum to come off at 4:45AM.

If anyone knows of any literature on decal effects on PAS staining in bone 
marrows please contact me.  This has been going on for months and no matter 
what we do manual staining, Leica adaptation for automated or Dako it is not 
helping.  Dako has been great with sending in technical experts repeatedly and 
we cannot get this corrected.

Thanks,
Pam

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Re: [Histonet] Histonet Digest, Vol 150, Issue 2

[Marcum, Pamela A via Histonet]

We have had troublemakers and rabble rousers on HistoNet before who just are 
not happy without causing everyone discomfort by their manners/words.  The more 
we feed this and complaint about the worse it will get for all of us.   We 
don't need to answer or get upset just delete and movew on.  This is the same 
as the school yard bully who needs attention anyway they can get it and now 
they can use the internet and words instead of fists and threats.  In this case 
it is a keyboard and something to make us mad.  DELETE and consider the source 
as my Grandmother would say, "Don't get in the gutter with them".   Histology 
has a great tool and interface for us here so let us focus on the positive 
more.  

Pam Marcum

-Original Message-
From: Hudson, Pamela R ASHVAMC via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, May 04, 2016 6:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2

OMG  This is terrible.  I can't believe I belong to a group of such 
unprofessional people.

Pamela R. Hudson, HT (ASCP)
Charles George VA Medical Center
Pathology Department
1100 Tunnel Road
Asheville, NC   28805
828-298-7911   x5747
pamela.hud...@va.gov

-Original Message-
From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 03, 2016 4:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2

Good to know that I am not the only on that think Rene is negative to others.  
Hey Rene it's not just one person that thinks your a dirt bag.


From: histonet-requ...@lists.utsouthwestern.edu
<histonet-requ...@lists.utsouthwestern.edu>
Sent: Tuesday, May 3, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2

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Today's Topics:

   1. No More Blog Posts --  Over and Out! (Lester Raff MD)
   2. IHC positive controls (Cindy Bulmer)
   3. Re: No More Blog Posts --  Over and Out! (Rene J Buesa)
   4. Fwd: (Linda Margraf)
   5. Re: No More Blog Posts --  Over and Out! (Manfre, Philip)
   6. Re: IHC positive controls (Morken, Timothy)
   7. Re: No More Blog Posts --  Over and Out! (Boyd, Debbie M)
   8. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
   9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
  10. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
  11. Re: Fwd: (Rene J Buesa)
  12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
  13. PAS/Decal Question (Marcum, Pamela A)


--

Message: 1
Date: Mon, 2 May 2016 17:39:28 +
From: Lester Raff MD <lr...@uropartners.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
 
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local>

Content-Type: text/plain; charset="us-ascii"

To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a
line at les.r...@post.com<mailto:les.r...@post.com>   The mailing list
is never used for any purpose other than announcing a new blog post. Be sure to 
let me know you are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203



--

Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer <cjbulmer2...@aol.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8


Hello Histoland,

I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?

1)  Cut (serial sections, stain the last slide for bugs) and oven time
(60) for 1 hr.
 then put slides in re

[Histonet] PAS/Decal Question

[Marcum, Pamela A via Histonet]

We are still having issues with our PAS stain on decaled bone marrows.  The 
Pathologists in HemePath are seeing what they refer to as smudginess in cells 
on some areas of the completed PAS slides.  We have looked at everything and 
cannot find where the issue is coming from at this point.  We have done manual 
staining for PAS, automated on the Leica stainer and on the Dako Artisan.  All 
methods show the same result for some slides.  We can go for several days to a 
week or more with no problem and then suddenly it is back and we have changed 
nothing in the way we do the processing, embedding, sectioning, 
deparaffinization and coverslipping.  We do as many as 38 bone marrow cores a 
night or as few as 8 and can find no correlation in the number we have to deal 
with for a given period.  All bone marrows drawn today must be completed by 8AM 
tomorrow morning.

Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a 
maximum of 7 hours +/-.

Grossed and placed in cassettes for 15 minute rinse in running DI Water

Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C

Rinsed in running DI water for 15 minutes

Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours 
minimum to come off at 4:45AM.

If anyone knows of any literature on decal effects on PAS staining in bone 
marrows please contact me.  This has been going on for months and no matter 
what we do manual staining, Leica adaptation for automated or Dako it is not 
helping.  Dako has been great with sending in technical experts repeatedly and 
we cannot get this corrected.

Thanks,
Pam

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[Histonet] Question About A Sponge Type

[Marcum, Pamela A via Histonet]

When I was at NSH in Austin, I talked to a vendor with a very different type of 
Histology.  It was very soft and far less porous than the normal blue sponges.  
Does anyone remember this or can you tell me where to get them?  I believe the 
company was from the UK with offices or distributors in the US.  I need to 
either buy or get some for a demo here with our pathologists.  We cannot use 
the blue sponges for many of our cases as they just too rough and the surfaces 
are too rough.

Thank You,

Pam Marcum
UAMS

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[Histonet] PAS Question

[Marcum, Pamela A via Histonet]

We have a problem with the cell membranes separating on our bone marrow slides 
on our PAS slides done daily.  We have tried everything to fix this and stop 
the problem.  We have tried manual staining, bulk staining on the Leica Stainer 
and the Dako Artisan and all have the same problem.  I am at a loss and the 
only thing left is the temperature of oven we dry slides in set at 65C.  We do 
up 38 bone marrows every night so this is critical for us.  If anyone can help 
please give us advise.

Pam

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[Histonet] HT/HTL Positions in Little Rock

[Marcum, Pamela A via Histonet]

Good Afternoon,

We have two openings in our Histology Laboratory at the University of Arkansas 
for Medical Sciences in Little Rock.  We have competitive pay and a sign on 
bonus for the right candidates.  The positions are for bench Histologist with 
experience however; we would consider someone who has their registry and may be 
new to the field.   The work is general Histology with special stains and no 
IHC.

Little Rock is located in Central Arkansas and is a beautiful place for all 
outdoor sports or hobbies as well as a well-rounded small city.  The cost of 
living is very affordable.

Please to the UAMS website at uams.edu and look for the employment or jobs 
area.  The positions are under Technical.  If you have questions please e-mail 
me at this address: pamar...@uams.edu

Thank You!!

Pamela Marcum
Histology Supervisor
UAMS AP Histology Dept.

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[Histonet] 2 Open Positions in Little Rock Arkansas

[Marcum, Pamela A]

WE currently have two open positions for either registered HT or HTL.  Registry 
must be current and some experience.  We are a heavily computerized Histology 
Laboratory and will require the persons applying to be comfortable with 
computers.  We are a routine Histology Laboratory at the University of Arkansas 
for Medical Sciences, in Little Rock.  This is a beautiful area in Central 
Arkansas with lots of things to do both indoors and especially outdoors.   If 
you are interested please contact me with your resume as soon as possible.


Please no recruiters, we are not allowed to use you,  I am sorry.

Thank You,

Pam Marcum

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Re: [Histonet] searching for a 50cm stainless steel profile A knife

[Marcum, Pamela A]

Try Delaware Diamond Knives and Dorn and Hart.  I believe both sell steel and I 
know they sell tungsten carbide.   Pam Marcum

-Original Message-
From: Eileen Akemi Allison [mailto:akemiat3...@gmail.com] 
Sent: Wednesday, June 10, 2015 7:25 AM
To: Maria Mejia
Cc: Histonet
Subject: Re: [Histonet] searching for a 50cm stainless steel profile A knife

Maria:
Hacker Instruments sells new and reconditioned knives. They also provide a 
knife sharpening service for a fee of course. I believe they have that size, 
but if not, they will help you. Here is their link.  
http://www.tidylabstore.com/newmikn.html

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com
H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com
Tele: (831) 375-3577 X117


 On Jun 9, 2015, at 8:28 PM, Maria Mejia mbmph...@gmail.com wrote:
 
 Hello,
 
 Can any one tell me where I can buy a 50cm stainless steel profile A 
 type knife to use on a tetrander cast iron sliding microtome?  Any 
 assistance from anyone will be greatly appreciated.
 
 Regards
 Maria Mejia
 UCSF
 Mission Bay
 San Francisco, CA
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Re: [Histonet] Specimen Accessioning .....

[Marcum, Pamela A]

Most places I have been use the block as numeric and the part as alpha; 
15SR1235 A (part) 1 (block) and etc.  I have not seen it cause an issue and if 
we go beyond Z as the alpha then we double back to an AA for the part and so 
on.  We just hope a resident doesn't go off track to often with the AA to 
whatever letter.  

Pam Marcum 
UAMS

-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org] 
Sent: Wednesday, June 03, 2015 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Specimen Accessioning .

We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and 
the corresponding paraffin blocks as A, B, C, etc.  We are in the process of 
changing this to letters for the different specimen parts and numbers for the 
blocks.  For those of you who do it this way, have you encountered any 
problems?  For example, if the specimen arrives and the different parts are 
numbered, do you simply convert them to letters?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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[Histonet] HT/HTL PRN Part-time to permanent Part-time or Full Time

[Marcum, Pamela A]

We have one part-time PRN positions open at UAMS in Little Rock AR and a 
full-time Histology position.  The person must be ASCP registered as either HT 
or HTL to qualify.  We are a very automated lab for special stains and routine 
staining, including coverslippers.  Computer skills will be needed as we are 
working with Epic Beaker for EMR.  The University of Arkansas for Medical 
Sciences had excellent benefits for employees.  We are not offering move 
assistance or bonuses at this time and I feel it is only fair to state that if 
you are interested and do not live in the area.

Please no recruiters as we are a state institution and are not allowed to use 
your services.

Thank You,

Pam Marcum

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Re: [Histonet] OJT Histotechs/Training

[Marcum, Pamela A]

I took my registry while I was doing neuroanatomical research on animals.  At 
the time I took it I was told do not use animal tissue or you will fail.  I had 
to find someone with a clinical Histology lab who would allow me to come in for 
my practical exam work.  I just had to prove myself when I wanted to get into 
clinical by working for a little less with the agreement that if I met the 
standard the pay and position would go up.  However; without the HT I would 
never have gotten through the door.  We have research people here at UAMS who 
are taking the exam because their PIs are willing to help them with a 
pathologist and have their work looked at for quality.  There are ways around 
the rules we sometimes have to bend a little.  

Pam Marcum

-Original Message-
From: Loralei Dewe [mailto:lld...@gmail.com] 
Sent: Monday, May 18, 2015 2:00 PM
To: Joelle Weaver
Cc: histonet@lists.utsouthwestern.edu; Mayer, Toysha N
Subject: Re: [Histonet] OJT Histotechs/Training

I have a different perspective on this issue. I have been in histology for over 
20 years. I worked at UC Davis in Vet. Histopath for several years. I was a 
histology Core facility manager and started up the facility from scratch at UC 
Davis Health system while running a Core Confocal microscope facility there. 
BUT I was in research, I wasn't in a Pathology lab and I don't qualify for 
the HT or HTL so I can't get work in the industry. Talk about a conundrum!

Loralei

On Sun, May 17, 2015 at 3:38 AM, Joelle Weaver joellewea...@hotmail.com
wrote:

 I will speak to my laboratory director about this. I know the 
 situation first hand from my previous experience!


 Joelle Weaver MAOM, HTL (ASCP) QIHC





  To: tnma...@mdanderson.org
  From: jmacdon...@mtsac.edu
  Date: Sat, 16 May 2015 20:02:34 -0700
  Subject: Re: [Histonet] OJT Histotechs/Training
  CC: histonet@lists.utsouthwestern.edu
 
  This is an issue with our program as well.  We have a difficult time 
  finding clinical sites for our students.  Many people want to hire
 trained
  individuals, but don't want to invest any time in the training.  Our 
  students receive a great deal of hands-on time in the student 
  laboratory, but need real life experience.
  Jennifer MacDonald
  Mt. San Antonio College
 
 
 
  From:   Mayer,Toysha N tnma...@mdanderson.org
  To: 'histonet@lists.utsouthwestern.edu'
  histonet@lists.utsouthwestern.edu
  Date:   05/14/2015 01:48 PM
  Subject:Re: [Histonet] OJT Histotechs/Training
 
 
 
  One good way to find techs is to offer to become a clinical 
  affiliate for a program.  Most programs struggle with attracting 
  students and providing them with clinical affiliates to fine tune their 
  skills.
  It may not matter that the school is not located near you, the 
  student
 may
  have family nearby to stay with.
  We are always looking for long distance affiliates, that way we can 
  attract an out-of-state student and not saturate the local area.  I 
  have students who want to relocate to different areas and just for a 
  change
 and
  this helps them do so.  We also get calls from applicants who don't 
  mind moving to us for 9-10 months, as long as they can go home when 
  they finish.
  If the program is agreeable to this, the specifics can be worked 
  out,
 such
  as what skills are entry level and the length of the time the 
  student is at your facility.
  Ours is called an Internship and the student is at the facility for 
  12 weeks.  They come in knowing basic embedding, cutting, routine 
  staining, specials, and have performed a minimum of three IHC 
  stains.  Two are manual and one automated.
  Some programs keep the students in house for some time before they 
  leave for internship, while others leave the technical training to the 
  clinics.
  It all depends on what is available.
  This would be a low cost way to see if you like a person, can train 
  them and are willing to teach.
  Some students are looking to relocate just before graduation, so a 
  move for an internship is a consideration.
  Many times it is the expectations of the trainer that are not 
  aligned
 with
  the skill level of entry-level techs and that can cause problems.  
  This way the person can come in with an assessment of the skill 
  level and the OJT phase can begin.  If the affiliate chooses to hire 
  the student,
 great.
   If not, then no harm.  At least you get to say that you tried and 
  did
 not
  have to waste money doing so.  It is not a source of free labor, but 
  a
 way
  of accurately assessing a person's fit for your needs.
  Many allied health programs (not just histo) are doing this and it 
  helps to showcase different labs and programs.
 
  Just my two cents.
 
  Sincerely,
 
  Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education 
  Coordinator Program in Histotechnology School of Health Professions 
  UT M.D. Anderson Cancer Center
  713.563-3481
 
 
 
 
 
  Message: 1
  Date: Thu, 14 May 2015 17:07:06 

Re: [Histonet] Friday Trivia Question: Most specimen on a single case

[Marcum, Pamela A]

157 blocks on one breast case - resident grossed it. 

-Original Message-
From: Michael Mihalik [mailto:m...@pathview.com] 
Sent: Friday, May 08, 2015 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Friday Trivia Question: Most specimen on a single case

Please excuse the trivia query, but we've got a client who somewhat regularly 
creates cases with 100+ specimen.  I think the most I have ever seen is 127.

I'm curious how common this is.  What's the most specimen on a single case 
you've ever seen?

Thanks for your patience and experience.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369



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[Histonet] Immediate Opening for HT or HTL in Little Rock AR

[Marcum, Pamela A]

We are currently looking for a register HT or HTL for full time bench work at 
the University of Arkansas for Medical Sciences in Little Rock AR.   The 
position will entail processing, embedding and staining for both routine and 
special stains.  The applicant should have experience with computers and 
Microsoft as we are very automated.  The position is for early morning.  Please 
let me know if you are interested.

If you are in Little Rock and looking for some PRN work please contact me ASAP. 
 This would also be very early morning only.

Recruiters please do not contact me as we cannot use your services due to state 
regulations.

Thank You,

Pam Marcum

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RE: [Histonet] prevent wrinkles when cutting

[Marcum, Pamela A]

How thick are you cutting?  Is the block cold?  Are you using disposable 
knives?  Are you moving the knife when you start sectioning?  How fast are you 
attempting to cut?
What kind of tissue are you cutting?  Do you know which paraffin is being used? 

There are many reasons for wrinkles and these are few of the questions we need 
to answer first.  

Pam Marcum 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M 
Gonzalez
Sent: Monday, April 20, 2015 11:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] prevent wrinkles when cutting

Hi

Thursday was the first time I ever used a microtome I move to a lab that 
does not have someone dedicated to cutting. I already miss her.

I have no problems getting ribbons of 10-30 sections long but the pieces are 
half the size of the original block. I am guessing they are wrinkling.
What am I doing wrong?

Thanks
Rachel
Senior Scientist
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[Histonet] HT or HTL Position in Little Rock AR

[Marcum, Pamela A]

We have an immediate opening for full time HT or HTL for early morning shift at 
the University of Arkansas for Medical Sciences in Little Arkansas.  We have 
great benefits, 2 weeks' vacation and sick leave.  Since it is a state facility 
we also have all Federal holidays and you birthday off.  The person will be 
required to have ASCP registry and computer knowledge.  We are a very 
progressive Histology Laboratory in the heart of Arkansas.  If you have any 
questions please e-mail me.  I am sorry we do not pay relocation or bonuses.

Please do not contact me if you are a recruiter as we are not allowed to use 
your service and I do not wish to waste your time or mine.

Thank You,

Pam Marcum

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[Histonet] RE: Re: Pneumatic Tube Delivery System

[Marcum, Pamela A]

Exactly how we handle it here.  Years ago they something biological spill in 
the tube system and the whole thing had to be decontaminated.  No one ever 
volunteered who did it, but it cost a fortune to clean up.  Still once in a 
whole someone will just have a brain hiccup and send something.  Everyone get 
upset then.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cates, Julia
Sent: Wednesday, March 25, 2015 7:59 AM
To: bcoo...@chla.usc.edu; histonet-boun...@lists.utsouthwestern.edu; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Pneumatic Tube Delivery System 

Hey Brian,

We do use the tube system at our hospital.  The rule here is that nothing in 
formalin is to be sent via tube.  The specimen is considered irreplaceable and 
in the event of a malfunction in the system there is the possibility of 
specimen loss.

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

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RE: [Histonet] sta on

[Marcum, Pamela A]

Not allowed in our lab at all.  We use charged slides for everything since we 
also cut IHC slides.  It is not good for IHC and can cause hideous background 
on slides.  We rarely have anything float.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
Sent: Wednesday, March 25, 2015 11:57 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] sta on

how many people use sta on in their waterbath?   we have not for years, but 
have a new person who wants me to get some, she is having trouble with the 
ribbons floating away.  

 

thanks for your input,

anita dudley

providence hospital

mobile, al
  
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RE: [Histonet] BS in Histotechnology

[Marcum, Pamela A]

In most cases it means nothing and if you are in Histology and the 
administration considers Histology a non-professional laboratory personnel area 
the pay is lower.  Sorry I have fought this for 5.5 years here and the 
difference between an HT and HTL is the degree only not the registry.  If it is 
$0.10 an hour it is good.  

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Tuesday, March 24, 2015 11:06 AM
To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology

So just out of curiosity is the pay on the same level as that of a Med Tech 
with a BS? 
Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of 
the on-line certificate programs?  

Tom 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Tuesday, March 24, 2015 11:47 AM
To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology

Jennifer, we require a BA/BS degree for all Histotechnologist positions. 
However, in our 4 step categories Level 1 does not require certification, just 
the degree and the requirement that they get the certification within a year. 
Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = 
entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 
is Lead tech). Supervisor requires and HTL.

Considering that we already require a BA/BS degree for all levels, the fact a 
person has a HT or HTL is not going to matter much for levels 1 thru 4, only 
for supervisor level.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Monday, March 23, 2015 7:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BS in Histotechnology

In what areas would a facility hire an HTL over an HT?  Is there a need for 
more HTL programs?  4 Thank you, ___
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RE: [Histonet] BS in Histotechnology

[Marcum, Pamela A]

Can we clone her? 

-Original Message-
From: Mark Turner [mailto:mtur...@csilaboratories.com] 
Sent: Tuesday, March 24, 2015 3:26 PM
To: Paula Sicurello; Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; 
Timothy Morken
Subject: RE: [Histonet] BS in Histotechnology

I once worked with a Pathologist who said she was in a group meeting of other 
pathologists when one of them blurted out that a trained monkey could cut 
slides.  My pathologist, having had the opportunity to review some cases from 
the offender's laboratory, promptly replied Yes, and with the quality of your 
slides it looks like you did just that.  She shut down the other pathologist 
really quickly, and as far as I know, we never received another case to review 
from him.  My pathologist was not about to let that kind of arrogance stand.  
She was one of the best bosses I ever had!

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, March 24, 2015 3:47 PM
To: Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; 
Timothy Morken
Subject: Re: [Histonet] BS in Histotechnology

I've had more than one pathologist tell me a monkey could do my job.
Though one of them said it with a smile and added a very highly skilled and 
well trained monkey, he was one of the few who knew better.

How many of us monkeys have trained the whining and complaining residents how 
to do things correctly?

Paula

On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones mjo...@metropath.com
wrote:

 OMG Pam~ our pathologist said the exact same thing to us when we 
 started our Grossing training.
 Sheesh. . .
 Michael Ann




 On 3/24/15, 11:52 AM, Marcum, Pamela A pamar...@uams.edu wrote:

 That was nicer than the pathologist who told me years ago, any 
 monkey could be trained to do my job.  I basically did not take the 
 job I was interviewing for at the time.  At least the next interview 
 went a lot better and I did take the job.
 
 Pam
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 Sanders, Jeanine (CDC/OID/NCEZID)
 Sent: Tuesday, March 24, 2015 12:30 PM
 To: Sue; Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: RE: [Histonet] BS in Histotechnology
 
 I agree, BUTas long as many pathologists think you can 
 teach any trained dog how to section histology will never have the 
 recognition those of us that have studied and trained deserve.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
 Sent: Tuesday, March 24, 2015 12:59 PM
 To: Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: Re: [Histonet] BS in Histotechnology
 
 This is a fight that we continue to have with hospital administration.
 In my opinion histologists are just as important and needed as MT.  
 Even though there is an increase in automation in pathology the hands 
 on of a histologists is most important.  The fact that hospital still 
 consider a lower entry job is the reason there are not more of us.
 It is quite frustrating.
 
 Sue
 TJUH
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 attachments, is for the sole use of the intended recipient(s) and may 
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 the intended recipient, please contact the sender by reply e-mail and 
 destroy all copies of the original message.


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RE: [Histonet] BS in Histotechnology

[Marcum, Pamela A]

That was nicer than the pathologist who told me years ago, any monkey could be 
trained to do my job.  I basically did not take the job I was interviewing for 
at the time.  At least the next interview went a lot better and I did take the 
job.

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 24, 2015 12:30 PM
To: Sue; Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: RE: [Histonet] BS in Histotechnology

I agree, BUTas long as many pathologists think you can teach any 
trained dog how to section histology will never have the recognition those of 
us that have studied and trained deserve.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 24, 2015 12:59 PM
To: Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: Re: [Histonet] BS in Histotechnology

This is a fight that we continue to have with hospital administration.  In my 
opinion histologists are just as important and needed as MT.  Even though there 
is an increase in automation in pathology the hands on of a histologists is 
most important.  The fact that hospital still consider a lower entry job is the 
reason there are not more of us.  It is quite frustrating. 
  
Sue 
TJUH 
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[Histonet] Posting for a Friend

[Marcum, Pamela A]

If you are interested in this position please contact Nephropath directly.


Nephropath is seeking a manager for its CAP accredited laboratory. Prospective 
candidates should hold a bachelor's degree as well as HTL or MT certification 
and have a minimum of five years of experience supervising in an accredited 
histology laboratory. The successful candidate will have proven technical and 
supervisory experience and exhibit an ability to exceed service, quality, and 
staff expectations. Salary will be contingent on a combination of qualification 
and experience.

Nephropath is a rapidly growing pathology practice founded in 2001 and located 
in Little Rock, AR. The cornerstone of the practice is providing same-day 
results for renal biopsies but is rapidly expanding into other pathology 
specialties and clinical testing. In 2014 Nephropath processed more than 10,000 
renal pathology cases.

If interested, please send your resume or questions to 
aaron.nich...@nephropath.com


Thank You,

Pam Marcum

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RE: [Histonet] RE: Masson Trichrome stain

[Marcum, Pamela A]

I have always bought saturated Picric Acid and not a specific strength.  Avoid 
making it from scratch!!  Pam 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nancy lowen
Sent: Friday, March 13, 2015 3:57 PM
To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Masson Trichrome stain

Just out of curiosity--what strength of Picric acid is in your Bouins 
fixative?Nancy 


 On Thursday, March 12, 2015 2:02 PM, Gayle Callis 
gayle.cal...@bresnan.net wrote:
   

 I have been following the string of inquiries about using metal forceps with 
Masson's Trichrome staining.  I was taught many years ago to avoid metal 
forceps or the older metal tissue cassettes with Bouins.  I am scrambling to 
find the actual reference.  The reason given was acids in Bouins corrode metal. 
 This may be a lost bit of information since the overall majority of labs now 
use plastic tissue cassettes.  Case in point:  using acidic descaling solutions 
for household cleaning i.e. showers/tubs or coffee machines.  These solutions 
come with warning to avoid metal fixtures and stainless steel sinks.  
Accidental contact of acids in a stainless sink causes the metal  discolor, 
indicating corrosion - been there, done that to a stainless steel sink.  I so 
use metal forceps to move slides between Mass Tri staining solutions (and 
silver staining solutions) without problems per John Kiernan's comment.         
 

 

Not using metal forceps with silver stains i.e. GMS, reticulin, is to avoid 
metal ion contamination which is more likely due to with poorly washed 
glassware.  In the past, we dipped metal forceps in melted paraffin, very messy 
since paraffin comes off on slides and in hot staining solutions.
Disposable plastic forceps are cheap but break easily resulting in a dropped 
slide.  Teflon forceps are pricey but it was a challenge to hold slides.
Hopefully there are teflon forceps that work better than the one we used?
We tried a teflon tipped metal forceps but not worth the price as teflon wears 
off the tips to rexpose metal.    Weigerts hematoxylin  is not affected by 
metal forceps since there are no acid components to corrode the metal although 
Weigerts can stain the forceps.  Simply wash the forceps in dilute chlorine 
bleach then soap and water.  I agree with John Kiernan and now use metal 
forceps to move slides between staining solutions in both Massons trichrome 
(and silver methods)  without problems.  If people want to use plastic or 
teflon forceps, I understand the reasons.      

 

As for not rinsing before going into Aniline Blue (or light green) in Massons 
trichrome, there is a reason for this.    Sheehan and Hrapchak state verbatim  
The phosphomolybdic acid and phosphotungstic acid thus acts as a link 
connecting basic groups of the connective tissue fiber to the basic groups of 
the dye i.e. aniline blue.  The PM/PT acid treatment has the ultimate effect of 
making an amphoteric dye that would ordinarily act as an acid dye to change and 
act as a basic dye.  These authors also say Although the exact mechanism of 
how the stain works is unknown, some theories are available.    By rinsing 
away the PT/PM, the link may be weaker hence one goes from PT/PM directly into 
aniline blue (sometimes light green or fast green).    Bierbrich Scarlet/acid 
fuchsin and aniline blue (light green or fast green) solutions can be filtered 
back and reused many times.  PT/PM and 1%  acetic acid solutions  should be 
discarded after use.


 

Instead of kits due to expense and some kit deviations from classic Massons 
Trichrome method, I found I could buy excellent, reliable single staining 
solutions i.e. Biebrich Scarlet/Acid Fuchsin and Aniline Blue from Newcomer 
Supply or Poly Scientific to avoid exposure when weighing out carcinogenic 
dyes.  Bouins is purchased from the vendor with the best price.  However, PT/PM 
and acetic acid single use solutions were still made in house to save costs.    
  

 

I strongly recommend reading John Kiernan's   Methods for Connective Tissues  
from his book , Histological and Histochemical Methods Theory and Practice  for 
better explanation and understanding of Massons Trichrome chemistry.    
Collagen and muscle staining methods in Sheehan and Hrapchaks Theory and 
Practice of Histotechnology is not recent but a good start.


 

Whew, a long reply but hope helps...

 

Gayle Callis

HTL/HT/MT(ASCP)  

 


 

Written is:  

 

Justine,

 

I do not have any metal forceps in the special stains area, due to the reaction 
that they can cause when staining with silver.  As a rule of thumb, it is just 
easier to use plastic all the way around.  

The Carson text does not state the use of only plastic forceps, but I would 
think that maybe they are concerned with a reaction between the Weigert's and 
the metal.  That would be a stretch.

RE: [Histonet] Embedding Question

[Marcum, Pamela A]

We routinely pull tissue from the processor and in the time it takes to load 
the embedding centers in numeric and alpha case order the block cool and 
paraffin will harden.  I would not call it freezing it has just cooled.  We 
start embedding immediately and between the heat on the embedding center and 
the heat in the block storage area on the embedding center we are fine.  On 
Saturday and/or Sunday we leave them in cooled paraffin until Monday morning, 
again no problem.  We do not refrigerate it everything is room temperature and 
then put in embedding center for approximately 30 minutes before we start 
embedding.  Sometimes for small biopsies we just set it on the staging area (10 
blocks at a time) and start as soon as the person assigned has the station set 
up for their preference.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Thursday, March 12, 2015 12:08 PM
To: Paula Sicurello
Cc: HistoNet; Morken, Timothy
Subject: Re: [Histonet] Embedding Question

If I may, I'd like to piggy-back onto what Paula has mentioned regarding 
allowing paraffin infiltrated tissue to cool before embedding it. Hopefully 
someone can help both of us out, even if we seem to warm our infiltrated tissue 
differently (Paula's in a dry bin and mine in a wax bath).

I work in a research lab where we work in large batches and time is not a 
priority like it is in a clinical setting. Rather than leaving 60-80 cassettes 
of infiltrated tissue soaking in a hot wax bath for hours at a time, we've 
begun to allow the cassettes to cool and just toss a handful of cassettes into 
the wax bath 5-10 minutes before we're ready to embed that batch of cassettes. 
Sometimes we don't even embed the cooled tissue until the next day or later 
that week. I haven't noticed an obvious difference in how our blocks section, 
though we have troublesome batches sometimes and we haven't been able to put 
our finger on why.

Anyone know if allowing infiltrated tissue to cool and then reheat before 
embedding is better or worse than keeping the tissue soaking in wax for hours 
at a time?

Thanks!
Lucie

Lucie Guernsey
UC San Diego
lguern...@ucsd.edu



On Thu, Mar 12, 2015 at 9:43 AM, Paula Sicurello pat...@gmail.com wrote:

 Hi Tim,

 There are several embedding events through-out the day, though mostly 
 in the wee hours of the morning.  The embedding centers would be in 
 the same room as the  microtomes (another question about those tomorrow).

 I worry about the small (GI, needles, etc) biopsies freezing before 
 they reach the embedding stations.  In my experience, once they freeze 
 they get this outer wax coating (like a permeability barrier) which 
 doesn't melt when placed in the dry (no paraffin inside) but hot, holding bin.

 They just don't seem to embed that well and have a tendency to drop 
 out of the sections when cutting.

 Has anyone else had that happen?

 Paula

 On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy 
 timothy.mor...@ucsf.edu
 wrote:

  Paula,
  How many times per day?
  Is the embedding close to the cutting area?
 
  Of course any extra walking is a problem, especially in busy areas. 
  Is this a non-patient area (hopefully!)? Any restructuring should be 
  to move things closer together, not further away!
 
  Having said that, If it comes to that I would be more concerned 
  about embedding proximity to the cutting area since having embedding 
  near
 cutting
  enhances workflow and cross coverage. If you don't unload processors 
  very often then having them distant might not be too bad. Not ideal, 
  but not a necessarily a deal killer.
 
 
  Tim Morken
  Pathology Site Manager, Parnassus
  Supervisor, Electron Microscopy/Neuromuscular Special Studies 
  Department of Pathology UC San Francisco Medical Center
 
 
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu [mailto:
  histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula 
  Sicurello
  Sent: Thursday, March 12, 2015 6:29 AM
  To: HistoNet
  Subject: [Histonet] Embedding Question
 
  It has been proposed to move the embedding centers to a room about 
  210 ft away from the tissue processors.
 
  The trip from processor to embedding center would take over 2 
  minutes and require the histotechs to carry the baskets full of 
  cassettes down a much used hallway.
 
  Opinions?
 
  Do you feel this is a good idea-yes or no and why?
 
  Thanks in advance,
 
  Paula
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[Histonet] RE: Old slides

[Marcum, Pamela A]

We have literally about one hundred slides to re-slip for the this reason.  Are 
there any suggestions for large numbers of slides to be re-coverslipped as this 
method would be too time consuming.   We have used only glass for about nine 
years or so and it is much better.  The old ones are the problem when someone 
needs THAT slide only.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Wednesday, March 11, 2015 10:43 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Old slides

Bernice,
Take the slide and dip it in xylene.  Lay it on the film, pressing down firmly. 
 As it adheres, then gently wipe the excess xylene off, and gently place it in 
a book or your procedure manual and leave it there for an hour or so.
Most of the bubbles will be gone, and the tissue will be saved.

The original problem is not enough xylene dispersed onto the slide.  Adjust the 
flow being dispensed by the unit.  

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator 
Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer 
Center
713.563-3481



--

Message: 1
Date: Mon, 9 Mar 2015 19:41:48 +
From: Bernice Frederick b-freder...@northwestern.edu
Subject: [Histonet] Old slides.
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
eb9d7062461640e5ac22d213a4acc...@evcspmbx03.ads.northwestern.edu
Content-Type: text/plain; charset=us-ascii

Hi all,
We received some old slides (1997-1998) that were coverslipped with film. 
Sakura I would imagine. The issue here is that the coverslips have come up from 
the slide and the tissue is adhered to the back of the coverslip. They need to 
be recovered so they can be evaluated. What do you all recommend? We use the 
CV5030 for coverslipping. I tried one with xylene and mounting media but there 
were still a couple of air bubbles in there.
Thanks,
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edumailto:b-freder...@northwestern.edu





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[Histonet] RE: Help with cutting mouse brain at 9-10 Microns

[Marcum, Pamela A]

Please give your processing schedule with times and reagents?  It is hard to 
help without knowing how the tissue was handled from sacrifice to embedding.  
Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly 
Marshall
Sent: Wednesday, February 18, 2015 8:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns

?Hello Histo folks


  I am starting a research project with mouse brain,  I am having trouble with 
chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all.  
I have soaked them in warm water and wonder in using a  softener like 
conditioner would help.  I am new to the animal tissue world and any advise 
would help.


Thanks in advance.

Kimberly
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[Histonet] RE: MICROTOMES

[Marcum, Pamela A]

We have six Leica RM2255 and they are great workhorses.  The automated controls 
are flexible as they can be on either side or on top of the microtome as needed 
or is most comfortable.  I personally like using the foot pedal for facing the 
blocks and I do get old fashion and cut my sections manually.  I can do it 
automatically just like to be hands on for the final steps.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breal, Kari
Sent: Tuesday, February 10, 2015 2:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MICROTOMES

I am interested in which automated microtome is the best for reducing ergonomic 
issues.  All opinions are welcome :)

Kari H. Breal, HT (ASCP)
Histology Manager
ABMC- 847-437-5500 x 5155
SAMC- 847-843-2000 x 6818
kari.br...@alexian.netmailto:kari.br...@alexian.net





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[Histonet] RE: Cloudy Superfrost Plus Slides

[Marcum, Pamela A]

Have you checked lot numbers and expiration dates?  I have only seen issues 
with slides that are stored in a very humid place or where the temperatures 
vary greatly over a year in storage.  We use  the ThermoFisher Colorfrost 
slides and have no problems here or where I lived in Pennsylvania.   

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Aono
Sent: Wednesday, February 04, 2015 2:35 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cloudy Superfrost Plus Slides

Have some cloudy Fisher Superfrost plus slides.  Sometimes they're cloudy even 
from boxes that are still in the plastic wrapping.  Does the wisdom of the 
collective histonet think these slides are okay to use or should be tossed?  
Thanks!

Michelle (Shelly) Aono
~~~
Research Associate II
107B/124 Greene Hall
Auburn University, Dept of APP
Auburn, AL 36849
(334) 844-5594

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RE: [Histonet] Problems with Universal Cassette Clamp

[Marcum, Pamela A]

I have been using RM2255 for almost 10 years and never had a clamp problem.  We 
have six in the lab with three of them more than 8 years old and other than 
routine PMs or the occasional human err problem these are great workhorses.  We 
keep contracts on them so we have great service.   I used one for about 4 years 
and cut plastic on it. The clamp was universal just not the same as the one on 
paraffin microtomes. 

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Rorick
Sent: Tuesday, February 03, 2015 11:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Problems with Universal Cassette Clamp

Hi Everyone,
I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the 
clamps have broken over the past year. 
Has anyone else had a problem with these? 


Anna Rorick
Anatomic Pathology Technician 
Battelle Memorial Institute
Columbus, Ohio
Sent from my iPhone
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[Histonet] ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH

[Marcum, Pamela A]

Good Morning All,

THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN 
LITTLE ROCK, ARKANSAS PLEASE JOU US!!

The meeting is being held at the Baptist Schools of Health in Little Rock and 
is open to anyone in Histology and related or interested fields.  The following 
list is for the speakers, who will all be presenting 90 minute seminars for the 
meeting.  We will have two rooms of speakers so you have a choice in classes 
and topics for the day.

7:30AM Registration

8AM to 9:30AM Seminars

Dr Ericka Olgaard from UAMS

Diagnostic Challenges of Breast Cancer

Dr Daisy Alapat UAMS

Methods and Basics of Protein Detection in Hematopathology

9:30AM to 10:00AM BREAK

10:00AM to 11:30AM  Seminars

Dr Shree Sharma Nephropath

Integrating Mobile Technology With Anatomic Pathology

Debbie Siena Technical Specialist Statlab

Controlling Your Special Stains

11:30AM to 1:00PM Lunch

1:00PM to 2:30PM Seminars

Dr Sara Shalin UAMS

How and Why Skin Is Different

Jennifer Feldman Ventana

Antibody Production

2:30PM to 3:00PM Break

3:00PM to 4:30PM Seminars

Dr Jennifer Forsythe ME Little Rock

Autopsy in America

Pamela Marcum HT UAMS

Reagent Alcohol - Can't Drink It - What Is It?







Thank You,

Pam Marcum

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[Histonet] Arkansas Society for Histotechnology Spring Meeting and HT Readiness All Day Class

[Marcum, Pamela A]

Good Morning!

We are holding our regular Arkansas Society for Histotechnology Meeting on 
March 7th, 2015, in Little Rock AR.  This will be a one day meeting with eight 
speakers on topic from the Medical Examiner' Office to general/IHC for the 
Clinical Histology Laboratory.  We are also offering an all-day HT Readiness 
class with Shane Jones as the presenter.  We would love to have any and all 
come to the meeting and join us for a day of learning and fun.

Exhibitors from many major companies will be there for the breaks and the Meet 
and Greet on Friday evening.  If you want further information please contact me 
at: ashn...@comcast.netmailto:ashn...@comcast.net and we will send out 
registration immediately and full programs this weekend or earlier.  The cost 
will $60.00 for the meeting and if you wish to join ASH an additional $20.00 
for one year.  The all-day HT Readiness will the same price of $60.00 for the 
day.  Lunch and 2 breaks will be provided as well as the Meet and Greet for 
this fee.

Thank You,

Pam Marcum

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[Histonet] Re: specials stainers

[Marcum, Pamela A]

We have used both and prefer the Dao hands down.  They are responsive if you 
have questions and very good staining.  We don't need to clean t after each run 
as it is all self contained and easy to use.

Pam

From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine 
(CDC/OID/NCEZID) j...@cdc.gov
Sent: Tuesday, January 20, 2015 5:58 AM
To: Mitchell, Janice A; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: specials stainers

Have not tried the Ventana but we are very happy with the Dako.


Jeanine H. Sanders, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
j...@cdc.gov



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specials stainers

Good Morning,
We are looking for an automatic stainer for special stains.   Ventana vs Dako?  
Thoughts?
Thanks for any input, Janice Mitchell
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[Histonet] RE: Recycling alcohol

[Marcum, Pamela A]

Hazel is correct if you use the pure ethanol the paperwork is horrible and the 
limits on what should be in the lab are very low for a normal Histology Lab.  
Reagent alcohol is the best way to go as it has methanol and isopropanol in it 
so it is undrinkable and therefore not under the same ATF rules of usage.  

Pam Marcum
UAMS 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, January 19, 2015 1:05 PM
To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol

With Ethyl alcohol you will need a license and will have to keep records.  With 
reagent grade alcohol none of that is necessary.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Monday, January 19, 2015 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycling alcohol

We are planning to recycle alcohol in the new lab I am working with.   
Previously I always used an alcohol blend such as the Flex products.  However 
at this new lab we are going to only process biopsies so I believe I can get by 
using ethanol and not a blend.   We will be getting our alcohol from 
Thermofisher.  Can anyone tell me which alcohols they are using for 
dehydration?  Reagent alcohol or ethyl alcohol.  Obviously we will make our own 
concentrations from a 100%.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com


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[Histonet] RE: specials stainers

[Marcum, Pamela A]

We use our Leica HE stainer for PAS only as it is the fastest way we have 
found to get 30 to 40 bone marrow PAS slides out before 6:30AM every morning.  
Otherwise it was just too cumbersome and the Dako does a great job on all 
routine stains.  We only do the neuro stains and Copper by hand now.  The Dako 
Copper kit is a different procedure and our pathologist like so it can't be 
used.  The PAS is just too slow for the turnaround we need daily.  The stain is 
great!!

Pam Marcum
UAMS 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Tuesday, January 20, 2015 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: specials stainers

I have the Leica multistainer.  We use it for H  E staining only.  When we 
attempted to set it up for Special Stains, all of the silver stains had to be 
made up either each run or each day.  Too much hassle.

Jeff Robinson, HT, HTL
Sierra Pathology Lab, Clovis, CA
jrobin...@pathology-associates.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, January 20, 2015 6:13 AM
To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: specials stainers

Oops   -  I had IHC on the brain and didn't pay close attention!

Leica does not have a dedicated special stainer  - but they have a multistainer 
which stains specials as well as HE. Is too slow in a busy lab tho.

I very much recommend the DAKO Artisan..

It is Monday on Tuesday... right?

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
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-Original Message-
From: Weems, Joyce K.
Sent: Tuesday, January 20, 2015 8:59 AM
To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu
Subject: RE: specials stainers

I recommend Leica.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specials stainers

Good Morning,
We are looking for an automatic stainer for special stains.   Ventana vs Dako?  
Thoughts?
Thanks for any input, Janice Mitchell
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RE: [Histonet] Levels for Gastic and Esophogeal Bx's

[Marcum, Pamela A]

We do 3 levels with no more than 2 sections per level.  It is what the 
pathologists prefer and therefore what we do.  

Pam Marcum 
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi 
Allison
Sent: Friday, January 09, 2015 8:07 AM
To: Histonet
Subject: [Histonet] Levels for Gastic and Esophogeal Bx's

Good morning Histoland:

Happy Friday!  I believe this question was asked last year, but I would like to 
revisit this again. I have worked in a variety of laboratories in my career, 
both small and large. Most of my experience was in Oregon with large hospital 
laboratories and University institutions. Back then, we cut (6) levels on all 
our bx’s, and picked-up (2) sections on each level.  My current facility is 
cutting (3) levels and picking up (1) section on each level.

I would like to ask what everyone else is doing.  The reason I am asking is 
because our pathology director is requesting a change in our protocol to cut 
only (2) levels and (1) section at each level.

Sincerely,

Akemi Allison BS, HT/HTL (ASCP)

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[Histonet] RE: A sticky subject....

[Marcum, Pamela A]

I am not sure where you live so this may or may not apply.  I have lived in 
several placed and one I learned no matter where I am when they turn on the 
heat and you wear anything with polyester or that can become electrostatic we 
had issues with static.  I am not sure how to fit it as I have made sure I 
touch something metal to discharge the static.  It does not cure the problem 
only humidity will and being careful not to start to cut fully charged so to 
say.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
Sent: Tuesday, December 30, 2014 7:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] A sticky subject

Good morning Histo folks,

 I am hoping somebody can help me. Today was a horrid microtomy day, 
the static was brutal. I tried putting lotion on my hands, the fabric softener 
sheet, and wearing gloves but to no avail. I heard rumors somebody used to 
manufactor an anti-static brush but have yet to find one. Does anybody have any 
other tricks that could be shared?

Cassandra Davis
cda...@che-east.org
302-575-8095



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RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta

[Marcum, Pamela A]

Thank you and all who help you for this service.  It is greatly appreciated and 
needed for all who are in the Histology field.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Margraf
Sent: Friday, December 19, 2014 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta 

 Dear Histonetters,
 In a couple of weeks, Histonet will celebrate its 19th anniversary! It is 
 hard to believe it has been in existence that long. I would like to say 
 thanks to all the members who contribute to the list, sharing their time and 
 expertise with others in histology-related fields. We currently have 3800 
 subscribers from at least 30 countries ( last time I counted).  It is amazing 
 to see how the list continues to provide new insights and information after 
 all these years.
 I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr 
 Sandy Cope as a co-administrator of the list with me. Anita is also a 
 pediatric pathologist and she will help with some of the issues with list 
 subscribing etc. Please continue to be patient with us if you are having list 
 issues as we often get busy in our day jobs. If your message to the list 
 doesn't post and it says it is because you are not a member, please see if 
 you need to update your email address on the membership page. Remember you 
 can only post to the list from an email address that matches exactly with one 
 on the subscriber list. You can always send us an email at the 
 histonet-owners address and we will try to help you out. Best wishes for a 
 happy and safe holiday season.
 Linda M
 Histonet administrator 
 
 Sent from my iPhone
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RE: [Histonet] RE: Angle of Wrist/Hands Using Microtome?

[Marcum, Pamela A]

One of our histologist uses a tennis ball on the end of his handle on the fly 
wheel.  He says it helps him keep his wrist level.  I can see it allows him to 
use less reach with his hand and his wrist is straighter than most.  I really 
need to try this myself someday.  I was trained wrist straight as possible and 
neutral if you wanted to avoid problems with you wrist and arm.  The automated 
microtomes are great for facing blocks as it cuts the shoulder action as well 
as lower arm.  

Pam Marcum
UAMS 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Monday, December 08, 2014 3:47 PM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu; Smith, Denise
Subject: Re: [Histonet] RE: Angle of Wrist/Hands Using Microtome?

OSHA states maintain neutral wrist and arm when working; work with their 
wrists in a neutral or straight

William DeSalvo
william.desa...@sonoraquest.ccom
602-768-3692
Sent from my iPhone

 On Dec 8, 2014, at 2:41 PM, Blazek, Linda lbla...@digestivespecialists.com 
 wrote:
 
 My two cents...  # 2  Keep your wrist straight.
 (you may get rich if you get enough 2 cents!)
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
 Chlipala
 Sent: Monday, December 08, 2014 4:33 PM
 To: Smith, Denise; 'histonet@lists.utsouthwestern.edu'
 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome?
 
 I think you want to keep your wrist straight so it would be 2.   Just my two 
 cents
 
 Liz
 
 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
 Boulder, CO 80308
 (303) 682-3949 office
 (303) 682-9060 fax
 (303) 881-0763 cell
 l...@premierlab.com
 www.premierlab.com
 
 March 10, 2014 is Histotechnology Professionals Day
 
 Ship to Address:
 
 Premier Laboratory, LLC
 1567 Skyway Drive, Unit E
 Longmont, CO 80504
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Denise
 Sent: Monday, December 08, 2014 1:53 PM
 To: 'histonet@lists.utsouthwestern.edu'
 Subject: [Histonet] Angle of Wrist/Hands Using Microtome?
 
 Hi all!
 
 When I took HTL exam and there was a question on the exam that bothered me 
 the most but I cannot find an exact answer for it, had to guess!  I'm very 
 curious about your thoughts on this.  I have asked Histology Core and they 
 weren't sure about it either - wanted to share it with you guys if you know 
 the official answer for this!
 
 The question was mainly about the angle of wrist/hands on the rotating 
 microtome and the degree must be correct so it won't cause any stress on 
 wrist/hands, it listed 4 different choices:
 
 
 1.   30 degrees wrist down forward
 
 2.   90 degrees straight without bending the wrist
 
 3.   60 degrees wrist up backward
 
 4.   60 or 90 degrees wrist down forward
 
 
 (Please be gentle with me since I don't remember the EXACT numbers but those 
 were close to numbers provided.)
 
 Thank you!
 
 Denise Smith
 
 
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[Histonet] RE: back orders from various vendors

[Marcum, Pamela A]

Happens every year at this time with various vendors and end of year.  I always 
over order in October to be sure I am good through the end of the year or as 
close as I can.  I know the things I use most and those are just what is always 
in backorder at the end of the year.  October became my answer as long as 
expiration dates are not an issue.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha 
Ward-Pathology
Sent: Tuesday, December 02, 2014 9:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] back orders from various vendors


I just need a moment to ventis it just me or is everyone seeing more and 
more back order issues with their various vendors?   It is not specific to any 
one particular vendor, product or even category of product, but lately I am 
being told more and more often that an item is not availableexpected 
release dates anywhere from a few days to 6-8 weeks!  This is starting to 
cause real problems .

Thanks for letting get this off my chest.I will resume my frantic search 
for another antibody vendor now. 

 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 
336.716.5890 mw...@wakehealth.edu  





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RE: [Histonet] Flames at embedding centers

[Marcum, Pamela A]

We have days when one of the histologist who has been here for years and I 
(going in 50years) start talking about the good days and everyone here is under 
40.  They look shocked and then disbelief and then they think we are kidding.  
They are so sure we have always known which chemicals were dangerous or killers 
and no one could possibly ever have been so careless.  WOW!  I like to show 
them old equipment and ask how they think it might work in a lecture on tissue 
processing.  They have no clue about an open processor in small room with no 
ventilation and we worked in it with our open stains lines and flames on the 
counter.  Now it does sound scary!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, November 18, 2014 3:04 PM
To: Jay Lundgren; Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Flames at embedding centers

Grinning and remembering the good old days.  What's more fun is the look of 
horror on the faces of the young ones when they hear it!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Tuesday, November 18, 2014 2:42 PM
To: Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flames at embedding centers

Ahh, the good old days of walking into the lab and lighting all the Bunsen 
burners first thing in the morning.  I didn't have any hair on my knuckles for 
years.  Embedding with a Coke on the cold plate, and a smoke in the ashtray 
next to you, anyone?  Good times.

 Now we have to use forceps warmers and change forceps between specimens.  
If you get 3 or 4 pair of forceps, one will always be hot enough to use.  Also, 
there are embedding centers with heated forceps, which I love .
 Just remember to clean out the wells of the forceps warmers every day to 
prevent cross contamination.  Cotton applicator swabs work great for this.  And 
always keep a towel or gauze handy to wipe the tips of the forceps between 
specimens.  Forceps warmers unfortunately don't incinerate any stray tissue 
like a Bunsen burner did.

   Sincerely,

   Jay A. Lundgren, M.S., HTL (ASCP)




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[Histonet] RE: Cold plates for icing blocks?

[Marcum, Pamela A]

Hi Tim,

We had looked at one time, for a smaller cold plate to be used at the microtome 
during sectioning and could not find anything suitable.  We were told that they 
would be very expensive and the one example I found was just that.  It would 
eliminate the water and mess of the melting ice if someone came up a cold plate 
that would hold say 30 to 40 blocks and could be a controlled temperature.  As 
always we need small to work in Histology!!! 

Pam Marcum
UAMS 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, November 03, 2014 1:29 PM
To: Histonet
Subject: [Histonet] Cold plates for icing blocks?

Does anyone use a cold plate, like that used for embedding, for icing blocks 
for sectioning? Just an idea

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org

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[Histonet] RE: working on Saturday

[Marcum, Pamela A]

We do have someone come in on Saturday for half a day.  We have an average of 
35 bone marrows a day and getting them cut and ready on Saturday is what we 
have to do to make Monday work.  This is a teaching hospital and the residents 
love to submit everything but the kitchen sink on Fridays and Saturday 
mornings.  We also have GI and Gyn clinics on Friday and the biopsy load goes 
up along with routine surgicals so Mondays can be very heavy.  The person 
working the half day Saturday can pick day during the week to leave early.  We 
have the same person on call for the week to cover RUSH transplant biopsies.  
(They rarely have to come in however; we still have to cover if needed.)  The 
on call person answers any problems with processors or other issues.  When we 
are full staff the cover is every fifth week, so it is not too bad.  I cover 
for any open spaces or health issues.  They prefer the supervisor not take call 
to avoid overtime.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Friday, October 24, 2014 8:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: working on Saturday

We are a 242 bed hospital with a very active maternity unit and a huge women's 
health service.  We get approx 10,000 cases/year. We find no advantage to 
working on Saturdays with the exception of a STAT cytology for an inhouse 
patient. Also, with the new recommended fixation times for breasts, we no 
longer even have one person for that little bit of Saturday coverage.
There was not one peep from any physicians.  Even when I used to manage a 
hospital lab twice the size, we did not cover Saturdays. 

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046

   1. Working on Saturday (Smallwood, Lorraine)
-



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Please notify the sender of any error by E-Mail.

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[Histonet] RE: Re: Working Saturday

[Marcum, Pamela A]

We give a half day during the week for the half day worked on Saturday.  This 
ended the overtime.   Since we start at 4AM generally the person on call will 
have some day during the week they want to leave early.  Since we have the 
schedule up before the first of the month most can make appointments or 
personal things for that half day.  It cut down on time off during work hours 
and allowed us to only pay call pay if the person is needed during an 
overnight.  Our shifts start at 4AM and we close at 4:30PM so they only cove 
the open hours.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Friday, October 24, 2014 10:52 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: Working Saturday

I applaud labs who have moved away from Saturday rotations. But I'm really at a 
loss to understand how it cuts costs? Don't most of you shift your work week 
for your staff if filling a Saturday rotation? So long as you don't pay OT for 
a Saturday, wouldn't the costs be the same?

Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)




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[Histonet] RE: processing and staining preserved specimen

[Marcum, Pamela A]

First of all THANK YOU for getting our field into a high school and teaching 
the kids.  This is a very good thing for all of us in the field.  

Could you give us an idea of the size of the specimen you are attempting to 
process?  A great deal depends on the tissue size as to how long it can be left 
in each reagent and paraffin.  I realize your limitation in time and did hand 
processing of monkey and rat brains as well as other tissue, for years.  No 
automated system was available and in research no money for one at the time.  I 
would be more than happy to share. 

Have you checked with TJ and maybe HUP to see if they old equipment that is 
still good from a research lab that might be available to be donated?  (Yes, I 
am from Philly)   

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Schade, Adelle
Sent: Friday, October 17, 2014 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processing and staining preserved specimen

Hello everyone,
I have been following and learning from this listserv for the past two years, I 
have enjoyed all of the advice this forum presents.  This is my first inquiry 
for the group.  I am a high school Anatomy and Physiology teacher and a grad 
student at Thomas Jefferson (Philadelphia, PA).  In our research lab, we 
process, section, embed and image for all research projects.  I was lucky 
enough to acquire some used histology equipment for my high school lab this 
school year.  I am teaching the students about histology and the processing.  
We have an embedding station, microtome, staining dishes and a good microscope 
to image.  We do not have an automatic tissue processor so we are processing 
manually (I prepare all solutions for the students).  We are taking biopsy 
samples from our preserved mink that we are dissecting at the moment.  I have 
processed the tissue using the following method and have pretty good results, 
not great.  (we are doing a basic HE stain to see the cells of the tissue).  
If we do not complete the  manual processing method and need to leave school 
for the day, I move the cassettes into 70% EtOH until the next morning when we 
continue the process.  I am wondering if anyone has manually processed 
preserved specimen tissue in the past and has any advice/ protocol that you 
would suggest?  This is what I am using:

Processing Method

1.  70% EtOH:  1 hour
2.  80%  EtOH:  1 hour
3.  90%  EtOH:  1 hour
4.  95% EtOH:  2 hours
5.  100% EtOH:  2 hours
6.  Histoclear II:  2 hours
7.  Paraffin:  2 hours

Also- I will eventually be looking for grants to try to purchase an automatic 
tissue processor- this would alleviate the time issues we are facing.  Does 
anyone know of grants that would focus on the educational aspect of histology?
Thank you for your time and have a great weekend!
Adelle Schade
Graduate student:  Jefferson School of Biomedical Science


Ms. Adelle L. Schade, B.S., M.Ed.
Anatomy and Physiology
Conrad Weiser High School
44 Big Spring Rd.
Robesonia, PA  19551
a_sch...@conradweiser.org



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[Histonet] RE: Shandon Excelsior ES®

[Marcum, Pamela A]

We have four Excelsior processors and love them.  We had some very old VIPs 
that we replaced with them over the past few years.  They are safer as the 
reagent handling and changing is quick and simple.  We dump the alcohol when 
the unit hygrometer (and the techs) see the percentage is low and ready to 
change, by allowing the unit to dump the dirty alcohol into an empty container 
in the lower half of the cabinet.  Then we remove the dirty bottle and cap it; 
while putting in a clean alcohol to be picked up and pulled in when the correct 
place is reached in the program.  It is the same with the xylene exchanges.  It 
dumps the paraffin when it is contaminated and you remove it, dump and reload 
in the empty chamber.  You set the number of uses for formalin and it counts 
down to a change and does the same dump and change out routine.   It saves us 
about 70% or more on reagent costs and would be higher if we were not using a 
Leica ASP 300s for our bone marrows.

It is easy to use and program and no they don’t pay me!!  It is safety first 
and less exposure to alcohol and xylene fumes is the real plus I wanted for the 
lab for everyone.   (No one complains anymore when we change reagents about the 
smell.  We share a large area with 4 other lab areas who are very picky!)   The 
rest we just learned to love.  We have had a few services calls however; Thermo 
answered fast and we up and running very quickly.  We use them every day and 
they are workhorses.   We have one for placenta and autopsy only, two for 
routine surgicals and one for biopsies only.  We also run biopsies and RUSH 
specimens during the day if they are available.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, October 16, 2014 9:23 AM
To: histonet@lists.utsouthwestern.edu
Cc: histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Shandon Excelsior ES®

What do people think about the Shandon Excelsior processor?  I currently have 
the old Miles-Sakura VIP and we love it but we are looking for another 
processor so we can have 2 processors for fatty tissue. I am ok with getting 
another refurbished VIP. I have excellent service contracted with a Bio Medical 
repair company they do all my PMs and repairs.  I'm trying to save money so I 
don't want to spend it where I don't really need it.  My mind frame is 'If its 
not broke don't fix it Any thoughts on this would be welcomed.
Thanks, Cheri

Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653


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[Histonet] RE: Histology Tracking systems

[Marcum, Pamela A]

We do about the same thing with the embedding and sectioning.  The computers 
are touch screen and some people use them, others use the pad on the keyboard, 
the rest asked for mice.  We have no way to get the information we need for 
sectioning from the system so, we have to do the paperwork by hand.  

The system we have was created by engineers who thought we should do 
processing, embedding and staining, then deliver the slides.  They missed 
sectioning completely and say they can't add it now.  If you are on check out 
you get credit for sectioning, staining and delivering every slide cut that 
day.  Just doesn't work well for workload recording daily.  

Someday maybe they will ask the people doing the work instead of assuming we 
are CP.  Nah, can't happen to easy.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, October 16, 2014 4:06 PM
To: 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Histology Tracking systems

Donna, we have touch screens, but our application is not fully touch screen 
friendly yet, so we have mini-keyboards that include a trackpad. They work well 
for login, choosing some items on the screen etc. They are not really meant for 
extensive use. 99% of the interaction is achieved simply by scanning a block or 
slide and using the touch screen as much as possible. the keyboard is just for 
that one percent in which the screen does not work so well.

We do all QA recording on paper at the microtome and embedding center, and then 
computer entry later. Again, the system is not set up well for that aspect yet. 

If you are thinking  of entering barcodes manually (you can do that if you know 
the ID) , yes, you can do it, it just slows things down  a lot.


Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Thursday, October 16, 2014 2:00 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Histology Tracking systems

I know this might be a strange question but, do any Histology Labs out in 
Histoland use a keyboard and mouse at Embedding and Microtomy to perform 
tracking, slide label printing and Quality Assurance recording.  Thanks in 
advance for your replies.

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


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[Histonet] RE: Floor Tiles

[Marcum, Pamela A]

We have really old floor tiles and do not allow Housekeeping to wax them with 
anything that would be slippery.  They use a non-skid wax only if they use 
anything.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, September 24, 2014 11:44 AM
To: 'Morken, Timothy'; 'Lester Raff MD'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Floor Tiles

We have a non wax floor.  It's a flat surface and easy to clean.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 24, 2014 11:40 AM
To: 'Lester Raff MD'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Floor Tiles

I don't think the ideal exists. Anything that is flat is going to have some 
slip issues, though the right floor wax can help, and maybe those peel-off 
sticky mats to catch dirt/paraffin. 

We have a vinyl tile floor that has a grid pattern embossed into it - 1 
squares. It is pretty much non-slip, but wax cannot be scraped off easily 
because of the embossed indentations. We need housekeeping to do some serious 
machine stripping and rewaxing every two weeks to clean it.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD
Sent: Wednesday, September 24, 2014 8:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floor Tiles

Our entire lab, including histology, has linoleum flooring. We cover areas with 
non-skid mats as necessary. 

 

We will be renegotiating our lease shortly and are considering having the 
building management company replace the flooring. Are there are any 
recommendations for a superior, non-slip, easy to clean and maintain flooring 
for the lab, particularly histology?

 

Thanks,

 

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

 

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RE: [Histonet] Whole rat head histology

[Marcum, Pamela A]

How old are the rats?  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William J. 
O'Connor III
Sent: Wednesday, September 17, 2014 10:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Whole rat head histology


I have a fun project - processing a whole rat head, skull with brain, for 
serial sections to see a brain/skull congenital deformity.Anyone have any 
experience with this?  I'd love it if you could share your protocol.  Paraffin 
or plastic?
Jackie O'


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[Histonet] RE: Automated Special Stainer...

[Marcum, Pamela A]

We love our Dako Artisan stainers and would not take the other brand back for 
any reason.  The techs love the ease of use and the pathologist love the 
stains.  What more could you ask!!  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 12:33 PM
To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!   I've used both systems (the Artisan in my old 
institution).  We currently have a few of the new Benchmarks on demo right now. 
 Thus far, we're not that impressed.  Frequently, there's variability on the 
silver stains (even in the same run,) and adjusting timing is nowhere near as 
flexible as on the Artisan.  Even if you take away the half hour for online 
deparaffinization, the stains take significantly longer on the Benchmarks (than 
the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The 
reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  
You'll need two Benchmarks to do what one Artisan does, TATs being equal.   
  

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sarah.dys...@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's 
North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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-


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[Histonet] HT Job Opening at UAMS IN Little Rock AR

[Marcum, Pamela A]

Good Morning,

We have a job opening at the University of Arkansas for Medical Sciences for a 
registered HT OR HTL.  We have great benefits, retirement and holidays for our 
employees and a relaxed, busy Histology Laboratory.  We have an active service 
with routine histology, special stains on the Dako Artisan and limited hand 
staining. This is an early morning shift.

Little Rock is a beautiful small city with lots outdoor attractions.  It is a 
beautiful area to live in and has a great low cost of living overall.  For more 
information you can e-mail me and send a resume.



Best Regards,

Pamela A Marcum
Supervisor AP Hisotlogy
University of Arkansas for Medical Sciences
4301 W Markham Street
Little Rock AR 72205

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[Histonet] RE: Me again

[Marcum, Pamela A]

We have 4 Excelsiors although the earlier non-touch screen version.  We love 
them and have had very few problems with them, generally just getting routine 
maintenance and taking good care for our maintenance is enough.  The reagent 
exchange is great with no problem there either.  We have multiple programs on 
each unit ranging from two hour biopsy runs to overnight and gout.  The 
agitation allowed us to slightly shorten several programs. 

Everyone loves the reagent changing as we are not pulling 12 bottles of 
solution out to dump at minimum, with our tissue load once a week on four 
units.   We set the formalin and clean cycles to the number we want to run and 
it tells us when that number is up and to change them.  The alcohol range is 
easy to read and know about when to change as is the xylene.  We reduced 
reagent costs by over 60 to 70% as we added more Excelsiors.  Now I just need 
to recycle and find a way to reduce cost for the HE stainers we have at the 
moment.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Monday, September 08, 2014 10:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Me again



From: Cheri Miller
Sent: Monday, September 08, 2014 10:31 AM
To: histonet-boun...@lists.utsouthwestern.edu
Subject: Me again

What are peoples thoughts on the Thermo Scientific Excelsior AS processor?

Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653


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[Histonet] RE: No food or drink in the lab

[Marcum, Pamela A]

I have not worked in or visited as technical representative since the middle 
70' that allowed in any food or drink in the lab anywhere.  If you have food or 
snacks they go in your locker and out of the lab or don't bring them in.  

In most labs this an automatic write up and can lead to progressive 
disciplinary action.  Your safety officer or department head should be all over 
this and if you don't have one check CLIA, CAP and any other letter 
organization to find it.  This is a health and safety issue that is taken 
seriously everywhere.  

If I find someone with food or drink in this lab they are disciplined before 
one of our safety officers from OHS finds out.  The fact that it is hidden or 
in cabinet does not cut it - No food or drink in the lab period. 

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:24 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states No food or drink in 
the lab and what the consequences would be?  That covers drinks/snacks in the 
cabinets above their workstations too, right? I have a sign up on the door 
entering the lab that states no food or drink, but the staff continue to keep 
stuff around their desk, so I need something to back me up from CLIA, OSHA, 
CAP, etc...

Thanks!



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[Histonet] Job Opportunity HT or HTL at UAMS in Little Rock AR

[Marcum, Pamela A]

We have a job opening for an ASCP registered HT or HTL in our active and modern 
Histology Department.  We are connected with one of the largest Multiple 
Myeloma Departments with numerous bone marrows per day as well as biopsies and 
routine surgicals.  We do routine staining and have automated our special 
stains with a few exceptions.  The person applying should be motivated and be 
able to see and pick up on whatever we need done during the day.  The shift 
will be 6AM to 2:30PM daily with one weekend every four to five weeks on call 
for Saturday and RUSH case (these are rare).  We are still growing and looking 
forward to a move soon and expansion. (IHC is done in another area.)

UAMS offers some great benefits and opportunities to go back to school with 
very generous discounts.  Arkansas is great place for outdoor activities and 
beautiful surroundings.   If you are interested please contact me by sending a 
resume and/or phone number so I can reach you to discuss your experience or you 
can ask me questions about the position.

Please recruiters: we are not able to use you for placements so do not answer 
this.  We cannot by state regulations use you for this position or in most 
areas of the hospital.


Best Regards,

Pamela A Marcum
Supervisor AP Hisotlogy
University of Arkansas for Medical Sciences
4301 W Markham Street
Little Rock AR 72205

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[Histonet] RE: biopsy bags for processing - alternatives

[Marcum, Pamela A]

One of our biggest issues is getting the residents and gross room staff to cut 
things appropriately.  We need the biopsies laying out flat on the wet inter 
bag and they seem to have issues refolding after the placement is completed or 
fold part into the bag distorting it.  We have tried to get them to pay more 
attention and decided to attempt to find another way if possible.  

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Norton, Sally
Sent: Wednesday, August 13, 2014 10:44 AM
To: 'Pam Marcum'; Timothy Morken
Cc: Histonet
Subject: [Histonet] RE: biopsy bags for processing - alternatives

We wrap our specimens in End Wrap papers (cut in half).  Takes more time I 
suppose than putting them in bags.

Sally Norton, HT
Seattle Childrens Hospital

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Wednesday, August 13, 2014 08:37
To: Timothy Morken
Cc: Histonet
Subject: Re: [Histonet] biopsy bags for processing - alternatives

Our kidney and liver biopsies are placed in BX bags (tea bags).  The 
pathologist feel the nylon bags leave a pattern on the tissue and sponges are 
even worse.  The Gross Room staff and residents also dislike the nylon bags as 
they feel they are harder to handle and stiff.  Then we in Histology feel 
exactly the same as Tim's description.  We have tried various things and keep 
going bag to tea bag style biopsy bags.  If anyone has come up with a better 
idea or product please let us all know.
Thank You,
Pam Marcum
UAMS

- Original Message -

From: Timothy Morken timothy.mor...@ucsfmedctr.org
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Wednesday, August 13, 2014 10:24:00 AM
Subject: [Histonet] biopsy bags for processing - alternatives

All knowing Histonet,

Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to pop open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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RE: [Histonet] biopsy bags for processing - alternatives

[Marcum, Pamela A]

We have no Histologist in the Gross Room and path assistants often do the 
biopsies as they meet CLIA guidelines for this.  The residents are required to 
do certain biopsies and we have no control over it.  The argument has been 
raging now for years and it is what it is so we live with it.  You and others 
may disagree (as I do) however; we are not the ones making those choices.  

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, August 13, 2014 11:55 AM
To: Morken, Timothy; Histonet
Subject: Re: [Histonet] biopsy bags for processing - alternatives

We never ever allowed the residents to wrap anything. They were instructed to 
leave everything in the sample bottle and later the histotech decided what to 
do. If there were many small pieces we filtered the sample through a tissue 
paper and processed it folded. Sometimes we used empty tea bags with very good 
results.
René J.  


On Wednesday, August 13, 2014 11:25 AM, Morken, Timothy 
timothy.mor...@ucsfmedctr.org wrote:
  


All knowing Histonet,

Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to pop open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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[Histonet] RE: questions

[Marcum, Pamela A]

We embed and cut and by priority for: 1) bone marrows, 2) biopsies, 3) breast 
cases  4) surgicals, 5) placentas, 6) autopsies.  RUSH cases for kidney, liver 
and heart take priority over all!  

Occasionally we will have requests for a specific case to be cut earlier than 
the usual priority list and will do that as needed.  

The cases are cut in numerical order for bone marrows and biopsies to make it 
easier to stain, organize and deliver them.  (We don't have to look for them 
for an hour if they are in order and anything missing is immediately apparent.) 
 Since we have four to five people cutting in the morning for surgicals we cut 
in numeric order by case and also alpha order again to help with staining, 
check out and delivery.  Depending on the person sectioning faster or slower we 
may have cases out of numeric order at times on the stainer and at check out.  

We adhere strictly to the breast times for 6 to 72 hours in formalin as minimum 
and maximum limits.  We would be prefer all breast have overnight fixation and 
that is not possible at times. We use the rule anything not in a cassette by 
3PM cannot be processed overnight for breast.  Then we know we have 6 hours 
based on time from gross to completion of formalin on the processors.  The 
trick was to make them understand it had to be grossed and in a cassette by cut 
off not just soaking in formalin.  We have residents.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Thursday, August 07, 2014 11:54 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] questions

I have a couple of questions to ask where there is no right or wrong answer, 
just curious as to the process that other labs use.
1.  After processing, how do you determine the order in which to cut and stain 
the blocks..numerical or priority driven?  2. Do you adhere to the 6-72 hours 
of fixation for breasts or make certain all breast tissue is fixed for a 
minimum time of, say, 24 hours but, of course no longer than 72?

I appreciate your responses and thanks for your time!!  I am retiring at the 
end of this year and trying to optimize some processes beforehand:).

Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962



  
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RE: [Histonet] Peggy Wenk's passing

[Marcum, Pamela A]

I am sorry to hear of our loss in the Histology world and more importantly of a 
truly good person in our lives. 

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Monday, July 28, 2014 8:48 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peggy Wenk's passing

It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday 
morning.  Her husband and companion of 37 years and her sister (from Bend,OR) 
were there.

As you know Peggy had a large influence in the world of Histology.

She also put herself into her church serving over the years as a nursery school 
director, Lay Eucharistic Minister and on the vestry. 
She sang enthusiastically in the choir and played in the newly formed bell 
choir.

Because of our love of books, we both volunteered at the local library.
We helped collect books, sort them and then helped to sell them; raising funds 
for the library's use.

There will be two memorial services: one here in Michigan for all her local 
family and coworkers, the second in southern California (a burial at sea).  We 
are asking that no flowers be sent; instead Peggy has specified (she and her 
sister planned the funeral several months ago) three different charitable 
organizations in lieu of flowers.

St. Mary's-in-the-Hills Episcopal Church (Peggy's church)

Shades of Pink Foundation (financial aid to local breast cancer patients)

Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University

Please respond to this email if you'd like more information on the services or 
on giving (or to l...@lpwenk.net).

Thanks
Lee Wenk (Mr. Peggy Wenk;)
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RE: [Histonet] problems Sllde Mate print

[Marcum, Pamela A]

Same here for printing staying on.  We use Thermo ColorFrost, slides have no 
problems.  It may depend on the finish the manufacturer uses for the paint on 
the slides. 

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Thursday, July 24, 2014 1:27 PM
To: Gudrun Lang; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] problems Sllde Mate print

Gudrun,



We have 2 slide mates and we run our slides through all reagents and do not 
have the issue you describe. The only issue we experienced was poor print 
quality due to the type of paint on the frosted end of the slides. I recently 
switched to Leica's charged slide that is designed for Thermal Printers like 
the slide mate and the print quality is top notch and does not fade, peel, or 
smear when touched and ran through xylene, alcohol, retrieval solutions etc



Part #
3800090 SLIDE WHITE Xtra Thermal



Are your ribbons old?

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang 
[gu.l...@gmx.at]
Sent: Thursday, July 24, 2014 2:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] problems Sllde Mate print

Dear histonetters,

we have problems with the stability of the print of our slide mates (Thermo).

We use regular slides with frosted end. The print usually looks good and stable 
at the time of printing.

But after staining the print comes off like peeled skin. Very easy to remove.



We take care, that the print doesn't come in contact with organic solvents 
(deparaffination reagens = Histo SAV, butyl acetate), but without any logical 
reason the first line of the print peels off and the second and third line is 
rather ok.



Has anyone seen similar problems and solved them?



Gudrun Lang



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[Histonet] RE: Eosin Leaching

[Marcum, Pamela A]

Have you changed your reagent alcohol or has the company you buy from changed 
where it purchases the reagent alcohol?  Reagent alcohols with higher volumes 
of isopropanol than  5% can cause the eosin to leach out.  It will leave a 
green powder in the bottom of you staining dish.  Methanol and Isopropanol 
should be 5% each with 90% ethanol for the safest blend and what most of us old 
folks always thought reagent alcohol would stay.  We were wrong and different 
companied can change vendors or sources and get something they were not 
expecting too.   If you look up reagent alcohol or SDA reagent alcohol you find 
it has many formulas and the only real requirement by the ATF is to make the 
alcohol non-drinkable or poison to humans.  I have seen formulas with 5 to 8% 
methanol and isopropanol with as low as 87% ethanol.  The percentage will 
depend on price as they have no idea at the manufacturing level what we use ti 
for or why.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos, Lori (VDH)
Sent: Monday, June 23, 2014 12:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Eosin Leaching

I too have been experiencing Eosin leaching. I have tried all different 
options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols 
and xylenes that follow, even added a running water wash step after the Eosin ( 
to remove excess). Before this, I have had no problems and had made no changes 
to my daily activities. I rotate solutions daily. Aside from changing the 
company I buy my Eosin from, I am at a loss.
Thanks in advance for your help.
Lori

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, June 23, 2014 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 127, Issue 28

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Contents of Histonet digest...


Today's Topics:

   1. RE: Eosin Leaching (Tony Reilly)
   2. Histology Benchmarking Survey (Lesley Bechtold)


--

Message: 1
Date: Mon, 23 Jun 2014 09:22:28 +1000
From: Tony Reilly tony.rei...@health.qld.gov.au
Subject: RE: [Histonet] Eosin Leaching
To: Rathborne, Toni toni.rathbo...@rwjuh.edu,   Sanders, Jeanine
(CDC/OID/NCEZID) j...@cdc.gov, 'AdrienneAnderson'
rennie1...@yahoo.com, 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Message-ID:

e8088c46632c1c489b85680dd5c0d76b028140c8a...@exccmsp10.qh.health.qld.gov.au

Content-Type: text/plain; charset=iso-8859-1

Hi Adrienne

Also make sure the wash after your bluing agent is higher than the blueing 
solution or alkalinity of the solution will leach out your eosin.

Regards
Tony


Tony Reilly B.App.Sc,  M.Sc
Chief Scientist
Anatomical Pathology
Pathology Queensland PAH
_
Health Services Support Agency| Department of Health

Building 15, Level 1,?
199 Ipswich Road?
WOOLLOONGABBA? Queensland 4102
Ph: 07 3176 2412
Mob: 0402139411
Fax: 07 3176 2930
Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au ? ? ?





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 
'histo...@listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Is your alcohol level as high as or higher than the level of eosin in the 
containers? If there is residual eosin left at the upper portion of the slide, 
that could be causing the problem.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem 

RE: [Histonet] Processors again

[Marcum, Pamela A]

We have 4 Excelsiors and love them.   We also have one Leica ASP300 and it is a 
great workhorse for us.   I think it just depends on your lab and what you like 
or what serves you best.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire
Sent: Thursday, June 12, 2014 11:13 AM
To: anita
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Processors again

I do not like the Excelsior, not very user friendly.  The VIP is good but my 
favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita 
azdud...@hotmail.com wrote:

 Sorry to bring this up again but wondering if anyone has used the Milestone 
 Logos One?  I have info on the Excelsior, and we curantly have a VIP.  Just 
 wanting to get ingo and thoughts on them all.  
 
 
 
 Thanks a bunch!!!
 
 
 
 Have a great day!!!
 
 
 
 Anita Dudley
 
 Providence Hospital
 
 Mobile, Al.
 
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RE: [Histonet] Was told I am having bounce-backs

[Marcum, Pamela A]

I have had the same issue and was told by HistoNet about the problem of 
bounces.  I did get some strange e-mails coming through on HistoNet that I 
deleted.  The problem was they opened in my end mail box due to my view 
settings before I could get rid of them.  I am wondering if this was the 
problem as I recognized no one on the bounced list I received.  I have gotten 
e-mails today.  

Pam Marcum 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Monday, April 28, 2014 2:59 PM
To: Histonet
Subject: [Histonet] Was told I am having bounce-backs

This is a test to see if this posts on histonet.  I was told yesterday that 
there are a number of bounce-backs. I haven't received emails from histonet the 
past few days.  Anyone else having problems?  Peculier issues with yahoo lately

Akemi Allison-Tacha, BS, HT (ASCP) HTL
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
W. Email: aalli...@montereygi.com
P. Email: akem...@yahoo.com
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[Histonet] RE: suppliers of reusable steel knives

[Marcum, Pamela A]

Try Delaware Diamond Knives or Dorner (Think).  There are several if you Google 
just Histology Steel Knives.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of idimi...@mun.ca
Sent: Wednesday, April 02, 2014 7:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] suppliers of reusable steel knives

Hello,

I am looking for suppliers of reusable stainless steel knives, the big ones, 
not the disposable kind.

If anyone is still using them and has information where I can buy them and 
sharpen the knives, it would be of great help for us.

Thanks,

Iliana Dimitrova, RT, B.Tech., M.Sc.

Histology Supervisor
Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine 
Memorial University of Newfoundland St. John's, NL Canada A1B 3V6



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[Histonet] RE: Retic stain kits

[Marcum, Pamela A]

I would try Polyscientific RD.  One thing we have an issue with on cost is the 
silver used in these kits is based on market value.  If the price of silver is 
up so are the prices of silver based kits. 

 Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Thursday, February 20, 2014 1:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retic stain kits

Hello Everyone,

Can anyone recommend a good retic staining kit that is reasonably priced and 
works well? Thanks,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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RE: [Histonet] Soaking artifact

[Marcum, Pamela A]

Thanks Peggy and Amen!  When you have people taking human tissue and facing 25 
or 30 blocks at a time and then leaving them face down on ice with water it 
causes the issues you stated.  Soaking is something that was far more common in 
the past as the tissue fixed on processors with less reagent (Technicons Monos, 
Duos and Ultras) and did not always come out well processed.  The fact that we 
would throw everything, no matter the size from tiny biopsies to huge hunks of 
tissue in was normal practice.  

We follow the practice the person is complaining about and only soaking for 
tissue with special requirements, such as bloody.  Everyone hated till they 
tried and realized it works better.  The pathologists are happier as the tissue 
is not water logged and stains better.

We have come a long way in the last 48 years I have been doing Histology and we 
need look at some old practices and update.  Lee did say rehydrate however; he 
also said be careful and do not allow the tissue to swell out of the block.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Monday, January 06, 2014 7:34 AM
To: Deanna Leslie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Soaking artifact

One slight amendment - this applies to human tissue.

Animal tissue has far less bound and unbound water to start with, so no matter 
how it's processed, it always ends up dry. Therefore, longer soaking in water 
is needed.

Peggy A. Wenk, HTL(ASCP)SLS

-Original Message-
From: Lee  Peggy Wenk
Sent: Monday, January 06, 2014 8:07 AM
To: Deanna Leslie ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Soaking artifact

The only soaking artifacts that I can think of would be caused by:
- soaking too long in water (minutes instead of a few seconds)
- soaking under-processed tissue in water

In both cases, the tissue is supposed to be protected by the wax, and if it 
is not (under-processed), or if the faced block is in water too long, the 
tissue can start re-absorbing water. The tissue then turns white and swells out 
of the block. So all that swelled out tissue is cut away and lost when the 
tissue is put back on the microtomy for sectioning the ribbon.

If you soak for just a few seconds, such as a gauze with water being held 
against the block on the microtome, after it has been faced, then you will get 
a little bit of water absorbed into just a few layers of cells. Just enough to 
cut 2-4 sections. And you won't see that swelling artifact.

For those of you saying - but I have to face all the blocks, put them back on 
ice and/or water while I cut a bunch more blocks, and then go back and cut each 
block - that is an artifact also. You have over-dehydrated your tissue during 
processing, and you are putting back the water that you should not have taken 
out. Processing is supposed to remove the unbound water (not attached to 
proteins), and some of the bound water (attached to proteins), and leave some 
of the bound water (attached to proteins) in the tissue. If you HAVE to soak 
EVERY block for more than a couple of seconds, then you are wasting time 
rehydrating and wasting time while microtoming. Cut down the time in the 
alcohols on the tissue processor, to leave a little bound water in the tissues. 
And you can NOT processing little biopsies on the same long processing cycle as 
the larger pieces of tissue (uterus, breast, etc.).
Those little biopsies will be over-dehydrated. They HAVE to be run on a 
separate cycle of much shorter time intervals (10-20 minutes in each solution 
(once fixed), instead of 45-60 minutes in each solution).

You should be able (on nearly every tissue block) to rough trim the tissue, and 
immediately start cutting ribbons. Possibly, you will need to put an ice cube 
on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the 
paraffin hardness to match the hardness of the tissue. That being said, some 
tissues are naturally brittle or crumbly, and always need some water put back 
in the tissue, such as spleen or bloody tissue, but again, some wet gauze on 
the faced block for a few seconds should be enough time to get 2-4 sections. 
And that's all the tissue we usually need from those blocks. If you need more 
for IHC, put the wet gauze back on the faced block, and cut a few more sections.

Peggy A. Wenk, HTL(ASCP)SLS

-Original Message-
From: Deanna Leslie
Sent: Sunday, January 05, 2014 4:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Soaking artifact

Has anybody in histoland ever heard of this?  I have been cutting tissue for 25 
yrs and until recently I had never heard of this!
I am under contract to a facility and the supervisor there does not want 
anybody to soak their tissue or use ice!  Your are supposed to use the cold 
plate, because as I have stated soaking them causing an artifact. I have 

RE: [Histonet] -80 degrees rationale

[Marcum, Pamela A]

Also we had time when finding a non-defrost freezer was difficult and lower 
temps were not only best; they were about the only way not to have unit turn 
off and on allowing tissue and antibodies to partially defrost.  Some of us 
learned that the hard way in early days of IHC.

Bill is correct in his answer about why it should be stored at the various 
temps also.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Wednesday, November 20, 2013 9:59 AM
To: Paula Sicurello; histonet; microsc...@microscopy.com
Subject: RE: [Histonet] -80 degrees rationale

Here is my stab at why -80 C.
 
Temperatures between 0°C and −25°C, the enzymatic activity of cells is only 
slowed but remains active. Below −40°C physiochemical exchanges are frozen. 
Cellular morphology is preserved at -80°C. Shelf life of tissue increases as 
the temperature drops. Once you get below -80°C you will need cryoprotectors 
and when you use cryoprotectors, temperatures must be below −130°C and will go 
as low as −196°C (liquid nitrogen).  Antibodies and proteins in solution are 
stable at −20°C.

William DeSalvo, BS HTL(ASCP)

 Date: Wed, 20 Nov 2013 09:25:54 -0500
 From: pat...@gmail.com
 To: histonet@lists.utsouthwestern.edu; microsc...@microscopy.com
 CC: 
 Subject: [Histonet] -80 degrees rationale
 
 Hello My Fellow Listers,
 
 The question of the day is:  What is the rationale for storing frozen 
 biopsies at -80 degrees?
 
 I have seen protocols that range in temperature from -40 to -80 degrees.
 
 Was -80 selected because that was the lowest freezers could go back in 
 the day?
 
 Awaiting your chilly responses!
 
 Thanks in advance,
 
 Paula
 
 --
 Paula Sicurello, HTL (ASCP)
 Supervisor, Clinical Electron Microscopy Laboratory Duke University 
 Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 
 27710
 P:  919.684.2091
 
 HIPAA Privacy Notification: This message and any accompanying 
 documents are covered by the Electronic Communications Privacy Act, 18 
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 individual (s) only. This information is confidential. If you are not 
 the intended recipient or an agent responsible for delivering it to 
 the intended recipient, you are hereby notified that you have received 
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RE: [Histonet] Immunohistochemistry for beginners

[Marcum, Pamela A]

Dako has some excellent training information for beginners and I think Leica 
may also have something.  If you talk to your Dako rep or contact them by phone 
or look online you should be able to get the information fairly quickly.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Monday, November 18, 2013 10:42 AM
To: Histonet
Subject: [Histonet] Immunohistochemistry for beginners

Hi fellow histonetters

I have a colleague that is completely new to immunohistochemistry. I have gone 
over what I could with him, however, I think that some literature would help 
him more. Do you guys know of any books or articles that could help a beginner 
like him. I do not have any on hand but would like to offer something.
 
 
I remain yours truly, 

Candice Camille 
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RE: [Histonet] Thermo equipment:

[Marcum, Pamela A]

We have had the Thermo system with the slide writers and cassette writers for a 
little over two and a half years.  We did have issues when we got the system as 
it was new to the market and had some bugs. Thermo fixed the problems and 
Thermo stood by us until we got everything we needed.  They did it very quickly 
to make us a very happy group of Histologists and the Gross Room techs.  

We now have two other labs who bought the system a year after we did and all 
the bugs were gone.  They have had no problems and love the systems as much as 
we do.  

We do decal nightly on bone marrows and have never had a cassette label fade or 
come off in the solution.  It was an absolute necessity for us to know the 
information did not fade and the cassette writer was the only I was comfortable 
would hold up for this as it literally burns the number into the plastic.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Monday, October 28, 2013 4:18 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thermo equipment:

Does anyone have experience/input on Thermo Scientific equipment?  Ex the slide 
labeling system (slideMate), processor, cassette printing system (printMate). 
We are considering purchasing this equipment and would like to hear as much 
input as possible. Good/bad, other suggestions, etc. 

Thank you-

Sent from my iPhone
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RE: [Histonet] Thermo equipment:

[Marcum, Pamela A]

We also have 4 Excelsiors in our area.  We replaced the first VIP processor 4 
years ago when it finally gave up on us.   I will say the VIP was over twenty 
years old when it died.  We then purchased the second Excelsior a year later so 
we have a dedicated biopsy unit.  Tragedy hit again as the last two VIPS (same 
age) died and we needed to have consistency in processing and training.  We 
have had very good success with the processors and any issues at all with 
service have been taken care of quickly.

They are great processors and the gently agitation helped us shorten some of 
our processes and the pathologist are much happier.  

Pam Marcum 
UAMS

-Original Message-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net] 
Sent: Tuesday, October 29, 2013 8:42 AM
To: Marcum, Pamela A; 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thermo equipment:

I ewcho Pam's message. Buggy at first, but stayed buggy for a long time w the 
cassette printer. They have replaced the heads on cassette writer a number of 
times, but we now have one that works great and we are happy with them. I have 
no experience w their processor. - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Tuesday, October 29, 2013 8:14 AM
To: 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thermo equipment:

We have had the Thermo system with the slide writers and cassette writers for a 
little over two and a half years.  We did have issues when we got the system as 
it was new to the market and had some bugs. Thermo fixed the problems and 
Thermo stood by us until we got everything we needed.  They did it very quickly 
to make us a very happy group of Histologists and the Gross Room techs.  

We now have two other labs who bought the system a year after we did and all 
the bugs were gone.  They have had no problems and love the systems as much as 
we do.  

We do decal nightly on bone marrows and have never had a cassette label fade or 
come off in the solution.  It was an absolute necessity for us to know the 
information did not fade and the cassette writer was the only I was comfortable 
would hold up for this as it literally burns the number into the plastic.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Monday, October 28, 2013 4:18 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thermo equipment:

Does anyone have experience/input on Thermo Scientific equipment?  Ex the slide 
labeling system (slideMate), processor, cassette printing system (printMate). 
We are considering purchasing this equipment and would like to hear as much 
input as possible. Good/bad, other suggestions, etc. 

Thank you-

Sent from my iPhone
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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[Marcum, Pamela A]

The rule here is a blade is cheaper than a cut.  Anytime you are walking away 
and returning within a few minutes to cut use the knife guard otherwise throw 
the blade out.  It is an accident looking to happen.  Recently we had a tech 
decide not use the knife guard and seriously cut himself by misjudging the 
distance from his elbow reaching an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

I was taught that when leaving your microtome for any length of time to always 
take the blade out. We had a tech that had the habit of leaving the blade on 
her microtome and even though she had the safety guard up someone from  
biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the next time 
then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a blade is 
used and taken out then it should be thrown away..because the more it is being 
handled the greater the chance of injury. Just saying what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use?

Left in but covered with the blade guard.  Not spanking new, but usable (for 
facing) get stored in an old box that the slides came in.  I like the slide 
mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health 
System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are 
covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you are hereby notified that you have received this document in error and that 
any review, dissemination, copying or the taking of any action based on the 
contents of this information is strictly prohibited . If you have received this 
communication in error, please notify us immediately by e-mail, and delete the 
original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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RE: [Histonet] Diamond Knives

[Marcum, Pamela A]

Delaware Diamond Knife has always been an excellent company to deal with on 
diamond knives and tungsten carbide.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Philip Slakmon
Sent: Wednesday, October 16, 2013 9:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Diamond Knives

Good Evening,

I would appreciate getting feedback on what you think of the different brands 
of Diamond Knives, pro's and con's quality, service, delivery, warranty, price, 
...


Thank you,

Philip
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RE: [Histonet] Thermo Fisher Slide Mate printer

[Marcum, Pamela A]

We use the Slide Mate and we really like them.  We did have one problem with 
the printer tape and then we got a different lot and things have been much 
better.  The slides we use are the Thermo Colorfrost slides, in 6 colours and 
we have had no issues with the ink smearing or coming off.  

Pam Marcum
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline
Sent: Thursday, October 10, 2013 2:58 PM
To: Moe, Barbi A; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Thermo Fisher Slide Mate printer

We are using them at Penn Medicine Dermatopathology.  We interface with our 
homegrown LIS.  We like the printers.  We have had some ribbon issues causing 
the print to move and some numbers to be cut off and the ink on the fisher 
slide can be wiped off so we are looking at other slides to see if they are 
more resistant to chemicals and oils with that printer, but overall we have 
been happy with them.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A
Sent: Thursday, October 10, 2013 3:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thermo Fisher Slide Mate printer

If anyone is currently utilizing this printer, could you please share pros and 
cons of your experience?  Specifically interested if anyone has the unit 
interfaced with Power Path computer system.



Thank you!



Barb Moe

Gundersen Health System

1910 South Ave

La Crosse WI 54601



ba...@gundersenhealth.orgmailto:ba...@gundersenhealth.org


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[Histonet] RE: Dress codes

[Marcum, Pamela A]

The hospital code for the laboratory is long pants, no open toed shoes, 
appropriate scrub or other tops.  The hospital does not provide the scrubs 
here.  We can buy what we want as long as they are not over the top.  Most 
hospitals have similar codes that are linked to safety and professional 
appearance.  Those who chose not to wear scrubs must still wear long pants, no 
open toed shoes and appropriate tops.  If you are working in the lab and do not 
have scrubs on a lab coat is necessary.  

As it was explained to me long ago, the long pants and closed shoes protect you 
from spills of reagents or fluids that could cause health issues or even burns 
in the case of acids.  The lab coats do the same and protect your clothing.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Tuesday, October 08, 2013 12:50 PM
To: 'Wineman, Terra'; Horn, Hazel V; 'Nancy Schmitt'; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Dress codes

Hospital dress code.  Scrub uniforms purchased through the hospital.  No open 
toed shoes.  None-uniformed employees are casual/business attire.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra
Sent: Tuesday, October 08, 2013 1:39 PM
To: Wineman, Terra; Horn, Hazel V; 'Nancy Schmitt'; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Dress codes

Regulatory agencies - OSHA, NIH, CDC for sure!!

Terra Wineman, HTL (ASCP)CM
Research Biologist
636-926-7476 phone
terra.wine...@novusint.com



-Original Message-
From: Wineman, Terra
Sent: Tuesday, October 08, 2013 12:35 PM
To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu
Subject: RE: Dress codes

In the research lab the dress code is an follows, close toed shoes this means 
no crocs or dress shoes with long pants, no shorts or dresses.  Safety glasses 
per OSHA and nothing loose that might get caught in machinery.

Terra Wineman, HTL (ASCP)CM
Research Biologist
636-926-7476 phone
terra.wine...@novusint.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Tuesday, October 08, 2013 12:23 PM
To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Dress codes

We have a hospital dress code.  It is not just for  the  lab.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Tuesday, October 08, 2013 12:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Dress codes

Not sure if my mail is going through or not...

Thank you

Nancy Schmitt


Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
205 Bluff Street
Dubuque, IA  52001
563-556-2010 ext. 142
nancy_schm...@pa-ucl.com



From: Nancy Schmitt
Sent: Tuesday, October 08, 2013 8:58 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Dress codes

Good Morning-
I am looking for input on dress codes - specifically shoe and pants guidelines 
and what regulatory agency your information comes from.
It seems this can be a hot button topic:( Thank you for your input- Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
nancy_schm...@pa-ucl.commailto:nancy_schm...@pa-ucl.com




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If you are not the intended recipient, please notify the sender that you have 
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RE: [Histonet] RE: Submitting Questions

[Marcum, Pamela A]

I have also piggybacked on another HistoNet e-mail when nothing else worked.  
Always with an apology to the original submitter.  Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Wednesday, September 25, 2013 3:01 PM
To: Douglas Porter; 'Nails, Felton'; 'nancy lowen'; 
histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology'
Subject: RE: [Histonet] RE: Submitting Questions

I bet that's it.  I re-submitted my question and took out all the logo's and 
fancy stuff after my name.  Hopefully it will now appear.

-Original Message-
From: Douglas Porter [mailto:doug.por...@caplab.org]
Sent: Wednesday, September 25, 2013 10:20 AM
To: 'Nails, Felton'; 'nancy lowen'; Joanne Clark; 
histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology'
Subject: RE: [Histonet] RE: Submitting Questions

I have .jpg files in my signature for initial emails but not in my reply 
emails.  I sent a test message yesterday and got the following reply from the 
server:

Your mail to 'Histonet' with the subject

Test Message!

Is being held until the list moderator can review it for approval.

The reason it is being held:

Message body is too big: 119656 bytes with a limit of 60 KB

Either the message will get posted to the list, or you will receive 
notification of the moderator's decision.  If you would like to cancel this 
posting, please visit the following URL:

 
http://lists.utsouthwestern.edu/mailman/confirm/histonet/9abe483614aeb7cc29e
e5aa890b57bc164701b31

I suspect the .jpg files are what is causing the problem as the message was 
only Test Message!  I will strip out the .jpg files and try again.


Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator
Cancer Registrar 

CAP-Lab, PLC 
2508 South Cedar Street 
Lansing, MI 48910-3138 

517-372-5520 (phone)
517-372-5540 (fax) 

doug.por...@caplab.org 

www.caplab.org  
 
 
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intended recipient, you are hereby notified that any dissemination, 
distribution, copying, forwarding or capture of this communication is strictly 
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immediately by return e-mail and delete this and all copies. Thank-you.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Wednesday, September 25, 2013 12:05 PM
To: 'nancy lowen'; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha 
Ward-Pathology
Subject: RE: [Histonet] RE: Submitting Questions

I have even tried copying the following link
(histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make 
a post. 

-Original Message-
From: nancy lowen [mailto:clayca...@yahoo.com]
Sent: Wednesday, September 25, 2013 10:49 AM
To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha 
Ward-Pathology
Subject: Re: [Histonet] RE: Submitting Questions

Me too!

On Tue, 9/24/13, Martha Ward-Pathology mw...@wakehealth.edu wrote:

 Subject: [Histonet] RE: Submitting Questions
 To: Nails, Felton flna...@texaschildrens.org, 'Joanne Clark'
jcl...@pcnm.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Date: Tuesday, September 24, 2013, 12:54 PM
 
 I have had the same problem over the
 past few months...
  
 Martha Ward, MT (ASCP) QIHC
 Manager
 
 Molecular Diagnostics Lab
 Medical Center Boulevard  \  Winston-Salem, NC 27157  p 336.716.2109  \  f
336.716.5890  mw...@wakehealth.edu
  
  
  
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Nails, Felton
 Sent: Tuesday, September 24, 2013 2:58 PM
 To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RE: Submitting Questions
 
 I am in the same situation??? 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Joanne Clark
 Sent: Tuesday, September 24, 2013 12:54 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Submitting Questions
 
 Can anyone out in histoland explain to me why my submitted  questions are not 
making it through to histonet?  I  submitted a question on the 19th of 
September, and I still  have not seen it appear in the group emails I get 
daily.  I sent the question to the correct address (I  checked it to be sure).  
When I respond to someones  question, I can see my responses, I just can't seem 
to get  an original question submitted.  Can anyone tell me  what I am doing 
wrong?
 
 
 
 
 Disclaimer: This electronic message may contain information  that is 

RE: [Histonet] The Good Old Days...

[Marcum, Pamela A]

I still can and learned from someone older than me years ago to save you any 
good cotton denim possible.  If you stretch taut and gently run the edge of a 
sharpened knife over it, the material will remove fine grit and further smooth 
the edge. 

I can still remember the first person I saw drop a knife off the knife 
sharpener and become the luckiest person alive.  She tried to catch it as a 
reflex and luckily caught on the heel not the edge.  We were all screaming drop 
it and jump back.  

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, September 20, 2013 1:15 PM
To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

How many can strop a knife?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias
Sent: Friday, September 20, 2013 2:01 PM
To: Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

Seems like no one has touched upon the fine art of knife sharpening. Sometimes 
I felt like an executioner sharpening my axe to the point of splitting a hair. 
Heaven forbid if you hit a staple. That part of the blade was no useless for 
days to weeks until the daily sharpening ground it out. Life is pretty good 
when you just pull out another blade from the plastic box.

Victor 

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB 244
Ninth  Jefferson
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
Privileged, confidential or patient identifiable information may be contained 
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attachments.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, September 20, 2013 10:40 AM
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Good Old Days...

Hear hear!  I agree and was just saying I love hearing the stories (although I 
am not young).  Thanks all for sharing these memories and lessons!

Sent from my iPhone

On Sep 20, 2013, at 10:13 AM, Davis, Cassie cda...@che-east.org wrote:

 I enjoy hearing sincere reminiscing...Even though us kids don't know how 
 good we have it, some of us enjoy having an old tech beside us on the 
 bench. I find weeding through the sarcasm can be profitable and in doing so 
 have learned so much. What the old techs did on a daily basis, we only did in 
 the practice lab and when the automated instruments and pre-made solutions 
 that we have come to rely on fail, experience is so very valuable. Only by 
 their blood, sweat and tears have we benefitted however, we have so far to 
 go, let's do it together.
 
 Cassandra Davis
 cda...@che-east.org
 302-575-8095
 
 
 
 
 
 Confidentiality Notice:
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 Health East and is intended for the sole use of the intended 
 recipient(s).
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 If you are not the intended recipient, please delete this message, and 
 reply to the sender regarding the error in a separate email.
 
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RE: [Histonet] Dimwits

[Marcum, Pamela A]

Thank you and well said!! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Friday, September 13, 2013 9:17 AM
To: anni...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dimwits

Hello Annieinarabia,
 
You are quite the advocate for Sir Rene J. of the most respected voices on this 
forum.
 
Truly if you want to be respected, give respect and do not degrade other as he 
does from time to time (in my opinion).  Give substance in your responses and 
not comment using some pompous response to a valid question.  Like I said to 
someone off line, its not that he doesn't have knowledge (so I here, don't know 
him personally) to answer with useful creditable answers just at time he 
doesn't. I am sure everyone appreciates his sharing of knowledge, just be 
constructive in your responses.
 
I don't comment on a subject unless I have useful information to share, never 
to belittle the person asking who is in search of a solution to a problem.  
Take that approach and less people will be annoyed with the responses.
 
FYI, there is only one dimwit attached to this response unless your suggesting 
everyone on Histonet are dimwits.  Also, not sure why you are attacking the 
youngsters on this forum, this forum is for the young, old and middle-aged.
 
Have a fantastic Friday
 
TiminTexas not Tim M. (Don't want anyone mad at him on accident)
 
Message: 13
Date: Fri, 13 Sep 2013 09:52:45 +0400
From: Anne anni...@gmail.com
Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: ab6084bc-fab0-44f0-8cc1-0d1c6f141...@gmail.com
Content-Type: text/plain;   charset=utf-8

My goodness ignorance abounds...Rene J is one of the most respected voices on 
this forum.
And 'she' is actually a 'he' you dimwits. 
I would take his advice above almost anyone on this forum. 
You youngsters should try to emulate rather than denigrate. 
Rene you rock!!!

Annieinarabia 



  
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RE: [Histonet] Re: Unregistered HT

[Marcum, Pamela A]

Thank you Bob!  I remember when pathology residents had to spend some time in 
histology learning at least enough to know we were a team not enemies.  
Residents now barely get to see the Histology Lab so expecting them to see us 
as anything other than labor is a part of the issue.  Their world is changing 
and I am not sure how it will play out for AP as whole.  

Deming was very sure as you stated that worker feedback and more importantly 
input into the process of every area of manufacturing was the key to having a 
both a creative and happy workforce.  Deming wanted to encourage creativity for 
the workers so they were part of the whole process.  We are rarely asked for 
input in any area of the work arena due to the tiered system that has developed 
in all areas of employment today.  It is usually the squeaky wheel that get the 
grease or in this improvements in any area of the workplace and life.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Thursday, September 12, 2013 8:22 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Unregistered HT

Since somebody mentioned the Samurai Pathologist (who is now 74 years old and 
in his 50th year in pathology) -

I agree with most of what's been said here and I won't repeat it.

From the pathologist's viewpoint - remember that most pathologists are 
now
on salary (or soon will be) and don't have a dog in the fight about doing the 
job as cheaply as possible.

I think that a very large part of the problem is that most pathologists haven't 
a clue as to what goes on in the histology lab (that's why we cram cassettes 
full of fatty breast tissue), and that pathologists need to acquire this 
knowledge in residency, to the degree that they can teach and trouble-shoot or 
work with senior technologists who can. It's particularly important that 
pathologists learn to embed.

Edwards Deming was an economist who grew up in operations research during 
World War 2. After the War he tried to get the automotive industry to adopt his 
methods. The executives laughed at him (and still do in the business schools, I 
think), so he took his ideas to Japan, where they built the Japanese automative 
industry. Deming's major idea (if I understand him
correctly) was that workers need constant specific feedback about what they're 
doing.

I think that the establishment of effective feedback from pathologist to 
histotechnologist is the first step in solving the problem we've been talking 
about. And I think that means a pathologist sitting down with a 
histotechnologist and reviewing some of the day's slides every blessed day.

Bob Richmond
Samurai Pathologist
Maryville TN
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RE: [Histonet] Equipment selection

[Marcum, Pamela A]

Actually the coverslipper from Leica is very good and can be linked to the 
stainer.  It is also a glass coverslipper not tape.  It is always about money 
and that may be the thing that decides no matter what any of prefers.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, September 12, 2013 11:08 AM
To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Equipment selection

If buying the Bond means that you are forced to swallow the Leica 
coverslipper go with Sakura and Dako.
If they are making that package is because the Bond sells well but they are 
having to swallow the coverslipper. Price is important but in the long run it 
is better to increase productivity and avoid problems. The Sakura is much 
faster than the Leica coverslipper.
René J.



From: Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
To: Rene J Buesa rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu 
Sent: Thursday, September 12, 2013 11:55 AM
Subject: RE: [Histonet] Equipment selection



I actually know all the equipment. Well, I know 3 of the pieces really well, 
the Leica Bond Max is new to me. I did go to another lab to see it as well as 
the Leica stainer/coverslipper. I was impressed with the Bond Max, not as much 
with the coverslipper part of the stainer/coverslipper. The big issue I am 
having is if I want to go with the Bond Max, I technically need to go with the 
Leica stainer/coverslipper because it is lumped into a big package deal. If I 
want the Sakura Prisma stainer/film coverslipper, I go with the Dako 
immunostainer. I am having trouble deciding. It is boiling down to money, which 
sucks since I might be forced to go with something that I might not be happy 
with in the future.
 
Haley
 
From:Rene J Buesa [mailto:rjbu...@yahoo.com] 
Sent: Thursday, September 12, 2013 5:32 AM
To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Equipment selection
 
I used the Sakura and the Dako, but it seems that the Leica Bond Max is getting 
popularity. Why don't you  go to a lab where they are in use and take a look?
RenéJ.
 
From:Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, September 11, 2013 4:46 PM
Subject: [Histonet] Equipment selection

Hi all,

I am trying to decide on what equipment to purchase. I have picked the 
processor, microtomes, embedding centers and a few other pieces, but now I 
still need to decide on the HE Stainer and the immunostainer. My options I 
have in front of me are for the HE stainer are Sakura Prisma stainer/film 
coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For the 
Immunostainer, I am looking at the Dako Autostainer and the Leica Bond Max. Any 
advice on any of these instruments would be greatly appreciated. I am trying to 
finalize my decision pretty quickly. Thank you in advance for any input.



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RE: [Histonet] Re: Histonet Digest, Vol 118, Issue 24

[Marcum, Pamela A]

Sorry I think that went out with me messing up spell check.  Hit the button too 
fast.  Anyway I am sure the point is there Dako - new - unit - better ability 
for IHC, ISH, CISH etc.   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey
Sent: Thursday, September 12, 2013 12:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24

O..o.oop! PC problem.

Haley,

I have experiences with all the above equipment's and here's my inf;

1) Leica HE Autostainer + glass coverslipper - smaller size therefore use 
lesser counter space

2) Lesser cost $$ for Leica HE Autostainer / coverslipper

3) Bond Max/Bond III could do IHC  ISH simultaneously in one Autostainer, 
while DAKO IHC autostainer can do only IHC (no heating elements)

4) Bond can do DELAY start while you are sleeping  continuos flow as well, 
while DAKO need removed slides after AR  run immediately (batches)

Madeleine Huey BS, HTL/QIHC (ASCP)

Supervisor - IHC  Histology (Pathology)


On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey madeleineh...@gmail.comwrote:

 From:Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
 To: histonet@lists.utsouthwestern.edu 
 histonet@lists.utsouthwestern.edu
 
 Sent: Wednesday, September 11, 2013 4:46 PM
 Subject: [Histonet] Equipment selection

 Hi all,

 I am trying to decide on what equipment to purchase. I have picked the 
 processor, microtomes, embedding centers and a few other pieces, but 
 now I still need to decide on the HE Stainer and the immunostainer. 
 My options I have in front of me are for the HE stainer are Sakura 
 Prisma stainer/film coverslipper and the Leica ST5020-CV5030 
 stainer/glass coverslipper. For the Immunostainer, I am looking at the 
 Dako Autostainer and the Leica Bond Max. Any advice on any of these 
 instruments would be greatly appreciated. I am trying to finalize my 
 decision pretty quickly. Thank you in advance for any input.

 Haley,
 I have experiences with all the above equipments and here's my inf;
 1) Leica HE autostainer with glass coverslipper - smaller size (use 
 lesser counter space) cheaper (buy 2 equivalent to 2 Sakura Prisma + 
 film coverslipper and smaller.


 On Thu, Sep 12, 2013 at 10:02 AM, 
 histonet-requ...@lists.utsouthwestern.edu wrote:

 Send Histonet mailing list submissions to
 histonet@lists.utsouthwestern.edu

 To subscribe or unsubscribe via the World Wide Web, visit
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 or, via email, send a message with subject or body 'help' to
 histonet-requ...@lists.utsouthwestern.edu

 You can reach the person managing the list at
 histonet-ow...@lists.utsouthwestern.edu

 When replying, please edit your Subject line so it is more specific 
 than Re: Contents of Histonet digest...


 Today's Topics:

1. RE: Equipment selection (Huggins, Haley - MRMC)
2. Re: Equipment selection (Rene J Buesa)
3. RE: Equipment selection (Huggins, Haley - MRMC)
4. RE: Equipment selection (Marcum, Pamela A)
5. Need your expertise (Webb, Dorothy L)
6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins)


 -
 -

 Message: 1
 Date: Thu, 12 Sep 2013 08:55:39 -0700
 From: Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
 Subject: RE: [Histonet] Equipment selection
 To: Rene J Buesa rjbu...@yahoo.com,
 histonet@lists.utsouthwestern.edu
 histonet@lists.utsouthwestern.edu
 Message-ID:
 
 4f36ec93a5737d4f8a2974e8fb8e260615f5698...@phx-msg-007-n2.chw.edu
 Content-Type: text/plain; charset=iso-8859-1

 I actually know all the equipment. Well, I know 3 of the pieces 
 really well, the Leica Bond Max is new to me. I did go to another lab 
 to see it as well as the Leica stainer/coverslipper. I was impressed 
 with the Bond Max, not as much with the coverslipper part of the 
 stainer/coverslipper. The big issue I am having is if I want to go 
 with the Bond Max, I technically need to go with the Leica 
 stainer/coverslipper because it is lumped into a big package deal. If 
 I want the Sakura Prisma stainer/film coverslipper, I go with the 
 Dako immunostainer. I am having trouble deciding. It is boiling down 
 to money, which sucks since I might be forced to go with something that I 
 might not be happy with in the future.

 Haley

 From: Rene J Buesa [mailto:rjbu...@yahoo.com]
 Sent: Thursday, September 12, 2013 5:32 AM
 To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Equipment selection

 I used the Sakura and the Dako, but it seems that the Leica Bond Max 
 is getting popularity. Why don't you  go to a lab where they are in 
 use and take a look?
 René J.

 From: Huggins, Haley - MRMC haley.hugg...@dignityhealth.orgmailto:
 haley.hugg...@dignityhealth.org
 To: histonet

RE: [Histonet] Re: Histonet Digest, Vole 118, Issue 24

[Marcum, Pamela A]

Note: Dako has a new Autostainer for IHC, IS, CASH etc. so it may wise to talk 
to them about that before making a decision.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey
Sent: Thursday, September 12, 2013 12:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 118, Issue 24

O..o.oop! PC problem.

Haley,

I have experiences with all the above equipment's and here's my inf;

1) Leica HE Autostainer + glass coverslipper - smaller size therefore use 
lesser counter space

2) Lesser cost $$ for Leica HE Autostainer / coverslipper

3) Bond Max/Bond III could do IHC  ISH simultaneously in one Autostainer, 
while DAKO IHC autostainer can do only IHC (no heating elements)

4) Bond can do DELAY start while you are sleeping  continuos flow as well, 
while DAKO need removed slides after AR  run immediately (batches)

Madeleine Huey BS, HTL/QIHC (ASCP)

Supervisor - IHC  Histology (Pathology)


On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey madeleineh...@gmail.comwrote:

 From:Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
 To: histonet@lists.utsouthwestern.edu 
 histonet@lists.utsouthwestern.edu
 
 Sent: Wednesday, September 11, 2013 4:46 PM
 Subject: [Histonet] Equipment selection

 Hi all,

 I am trying to decide on what equipment to purchase. I have picked the 
 processor, microtomes, embedding centers and a few other pieces, but 
 now I still need to decide on the HE Stainer and the immunostainer. 
 My options I have in front of me are for the HE stainer are Sakura 
 Prisma stainer/film coverslipper and the Leica ST5020-CV5030 
 stainer/glass coverslipper. For the Immunostainer, I am looking at the 
 Dako Autostainer and the Leica Bond Max. Any advice on any of these 
 instruments would be greatly appreciated. I am trying to finalize my 
 decision pretty quickly. Thank you in advance for any input.

 Haley,
 I have experiences with all the above equipments and here's my inf;
 1) Leica HE autostainer with glass coverslipper - smaller size (use 
 lesser counter space) cheaper (buy 2 equivalent to 2 Sakura Prisma + 
 film coverslipper and smaller.


 On Thu, Sep 12, 2013 at 10:02 AM, 
 histonet-requ...@lists.utsouthwestern.edu wrote:

 Send Histonet mailing list submissions to
 histonet@lists.utsouthwestern.edu

 To subscribe or unsubscribe via the World Wide Web, visit
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 or, via email, send a message with subject or body 'help' to
 histonet-requ...@lists.utsouthwestern.edu

 You can reach the person managing the list at
 histonet-ow...@lists.utsouthwestern.edu

 When replying, please edit your Subject line so it is more specific 
 than Re: Contents of Histonet digest...


 Today's Topics:

1. RE: Equipment selection (Huggins, Haley - MRMC)
2. Re: Equipment selection (Rene J Buesa)
3. RE: Equipment selection (Huggins, Haley - MRMC)
4. RE: Equipment selection (Marcum, Pamela A)
5. Need your expertise (Webb, Dorothy L)
6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins)


 -
 -

 Message: 1
 Date: Thu, 12 Sep 2013 08:55:39 -0700
 From: Huggins, Haley - MRMC haley.hugg...@dignityhealth.org
 Subject: RE: [Histonet] Equipment selection
 To: Rene J Buesa rjbu...@yahoo.com,
 histonet@lists.utsouthwestern.edu
 histonet@lists.utsouthwestern.edu
 Message-ID:
 
 4f36ec93a5737d4f8a2974e8fb8e260615f5698...@phx-msg-007-n2.chw.edu
 Content-Type: text/plain; charset=iso-8859-1

 I actually know all the equipment. Well, I know 3 of the pieces 
 really well, the Leica Bond Max is new to me. I did go to another lab 
 to see it as well as the Leica stainer/coverslipper. I was impressed 
 with the Bond Max, not as much with the coverslipper part of the 
 stainer/coverslipper. The big issue I am having is if I want to go 
 with the Bond Max, I technically need to go with the Leica 
 stainer/coverslipper because it is lumped into a big package deal. If 
 I want the Sakura Prisma stainer/film coverslipper, I go with the 
 Dako immunostainer. I am having trouble deciding. It is boiling down 
 to money, which sucks since I might be forced to go with something that I 
 might not be happy with in the future.

 Haley

 From: Rene J Buesa [mailto:rjbu...@yahoo.com]
 Sent: Thursday, September 12, 2013 5:32 AM
 To: Huggins, Haley - MRMC; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Equipment selection

 I used the Sakura and the Dako, but it seems that the Leica Bond Max 
 is getting popularity. Why don't you  go to a lab where they are in 
 use and take a look?
 René J.

 From: Huggins, Haley - MRMC haley.hugg...@dignityhealth.orgmailto:
 haley.hugg...@dignityhealth.org
 To: histonet@lists.utsouthwestern.edumailto:
 histonet@lists.utsouthwestern.edu 
 histonet

RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

We have heard for years that NSH is working on changing the status to 
Laboratory Professionals and so far as I have heard nothing happening to make 
the change.  A large part of this is ASCP/all organizations 
inspecting/licensing are still looking at us as non-skilled labor that requires 
only OJT training or an AA/AS degree at best.  We have a few one year schools 
and some online however; not enough to cover the need for HTs and HTLs.  

We need ASCP/CAP/CLIA/all organizations that inspect us and tell us how to run 
the laboratories to actually understand we have changed in the last 25 years 
and no longer just cut and do HEs.  I am not sure where to go as I have been 
bringing this up for years and see no movement.  Take the test to be an 
inspector for CAP and you will see the problem clearly.  It is 98% Clinical 
Laboratory and maybe 2% Anatomic Pathology.  If you don't know Clinical you 
will have a problem passing the test without help.

Pam Marcum

-Original Message-
From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] 
Sent: Wednesday, September 11, 2013 8:08 AM
To: 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

And the reason so many have been fighting for this for years. If a lab were 
looking for a Medical Technologist there would be no question.


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Tuesday, September 10, 2013 7:32 PM
To: Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

As long as we do not need certification, licensure and minium education 
requirements we will not be recognized as Laboratory Professionals.



From:   Marcum, Pamela A pamar...@uams.edu
To: 'joelle weaver' joellewea...@hotmail.com, 'Emily Sours'
talulahg...@gmail.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date:   09/10/2013 01:12 PM
Subject:RE: [Histonet] Unregistered HT
Sent by:histonet-boun...@lists.utsouthwestern.edu



I agree we have huge gray areas and not all histology schools are as good as 
they could be for what we are facing in Histology.  I keep harping on the fact 
that until we are recognized as Laboratory Professionals we will stay in this 
limbo.  The rules determining complex testing should be revisited to what is 
done in Histology Laboratories today and not what we did 30 or more years ago.  
The Clinical Laboratory is now so automated it is hard to find anyone in most 
areas who can even remember doing any manual testing.  The Micro lab is the 
closest to being as manual as areas of Histology.

I am in a small market and finding a registered Histologist is harder for us.  
I would love to have 8 to choose from and interview.

Pam Marcum

From: joelle weaver [mailto:joellewea...@hotmail.com]
Sent: Tuesday, September 10, 2013 2:59 PM
To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

 Well I am  mostly clinical...but I think that organizations can set standards 
outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I 
had to submit all my transcripts from high school up through masters in 
addition to  proof of my ASCP certification, IHC qualification, continuing 
education, and professional association activity. There is a lot of gray area 
out there. They seem to have not had trouble getting applicants though ( and I 
know this varies by market), there were over 8 candidates for an HT opening, 
which I thought was a pretty good turn out.


Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: pamar...@uams.edu
 To: talulahg...@gmail.com; joellewea...@hotmail.com
 CC: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Unregistered HT
 Date: Tue, 10 Sep 2013 13:53:26 +

 Research is a different area and not controlled by CAP, CLIA and other
hospital licensing or accreditation organizations. We are bound by the rules of 
these organizations and while I agree with you to a point. We do need minimums 
for training and registration by recognized licensing bodies when patient 
tissue is being processed for histological examination. I am sure

RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

You are absolutely right.  Pathologists are afraid if they are not seen as the 
ones doing complex testing the last bastion of their power will disappear.  
It would help if they just realize how much we do to help them by knowing how 
to read and judge whether those complex tests are done right and the correct 
controls in place to keep them from saying or reading the wrong thing.  Many 
pathologists will admit they could not do our job or even understand what we do 
to get the tissue from a cassette to a stained slide.  I think some think we 
wiggle our noses and it appears like magic stained on the slide.  

If they can't gross well, then we know where the true understanding of 
Histology starts.  They managed to get a registry for Pathology Assistants that 
is quite hard to pass so they could stop grossing in many hospitals.  Now we 
have some good and some bad PAs who are of the same opinion as the pathologists 
about Histologists in many cases.  We have CLIA approved (based on education 
and number of science hours) as the cost of PA is too high in many AP labs.  

Pam Marcum.

-Original Message-
From: Horn, Hazel V [mailto:hor...@archildrens.org] 
Sent: Wednesday, September 11, 2013 8:30 AM
To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

Histology does not get the respect or the recognition because histologists do 
not report results.  All of the complex testing we do is overlooked because the 
pathologists report the results.   CLIA standards are based on result 
reporting.   The CAP has looked the other way for years because pathologists 
would hire unregistered techs.  If pathologists would demand only registered 
techs half our battle would be won. 

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Wednesday, September 11, 2013 8:08 AM
To: 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

And the reason so many have been fighting for this for years. If a lab were 
looking for a Medical Technologist there would be no question.


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Tuesday, September 10, 2013 7:32 PM
To: Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

As long as we do not need certification, licensure and minium education 
requirements we will not be recognized as Laboratory Professionals.



From:   Marcum, Pamela A pamar...@uams.edu
To: 'joelle weaver' joellewea...@hotmail.com, 'Emily Sours'
talulahg...@gmail.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date:   09/10/2013 01:12 PM
Subject:RE: [Histonet] Unregistered HT
Sent by:histonet-boun...@lists.utsouthwestern.edu



I agree we have huge gray areas and not all histology schools are as good as 
they could be for what we are facing in Histology.  I keep harping on the fact 
that until we are recognized as Laboratory Professionals we will stay in this 
limbo.  The rules determining complex testing should be revisited to what is 
done in Histology Laboratories today and not what we did 30 or more years ago.  
The Clinical Laboratory is now so automated it is hard to find anyone in most 
areas who can even remember doing any manual testing.  The Micro lab is the 
closest to being as manual as areas of Histology.

I am in a small market and finding a registered Histologist is harder for us.  
I would love to have 8 to choose from and interview.

Pam Marcum

From: joelle weaver [mailto:joellewea...@hotmail.com]
Sent: Tuesday, September 10, 2013 2:59 PM
To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

 Well I am  mostly clinical...but I

RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

Many states and regions are affiliated with NSH and have state societies that 
have annual meetings with one or two days of seminars and workshops that are 
reasonably priced and hopefully close enough to allow attendance.  The National 
Society for Histotechnology has a meeting at a specified location every year 
that is 5 days long and covers clinical, veterinary, research and other topics 
in management.  The meetings are listed on the NSH.org website if you are 
interested in looking at all available meetings for most states and regions.  
These are great educational opportunities for everyone whether you are a 
registered HT/HTL/student or researcher with special interests.

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Wednesday, September 11, 2013 9:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: Fwd: [Histonet] Unregistered HT

How do you gain credits? Do you get them for attending seminars or do you have 
to take actual courses?
Are these the CME's I always see noted for lectures? I never knew what those 
were for.

Emily

By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward.

-Chuck Palahniuk, Haunted


On Wed, Sep 11, 2013 at 10:46 AM, Marcum, Pamela A pamar...@uams.eduwrote:

 Agreed and now that the registries for HT and HTL require credits to 
 remain registered it does help with keeping people in the area 
 learning and growing.  The point that other than the front office for NSH is 
 a well
 taken point and the rest are volunteers.   I am not always sure the front
 office understands hospitals, private labs, universities etc. are not 
 sending anyone to meetings or really encouraging further education for 
 most lab staffs.  Histology here can get registration paid for and 
 everything else is on the person attending for transportation/room and 
 board and all other expenses we are not able to go to meetings in far flung 
 places.

 Pam Marcum



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Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or 
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message.
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RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

Agreed and now that the registries for HT and HTL require credits to remain 
registered it does help with keeping people in the area learning and growing.  
The point that other than the front office for NSH is a well taken point and 
the rest are volunteers.   I am not always sure the front office understands 
hospitals, private labs, universities etc. are not sending anyone to meetings 
or really encouraging further education for most lab staffs.  Histology here 
can get registration paid for and everything else is on the person attending 
for transportation/room and board and all other expenses we are not able to go 
to meetings in far flung places.  

Pam Marcum

-Original Message-
From: Mitchell Jean A [mailto:jmitch...@uwhealth.org] 
Sent: Wednesday, September 11, 2013 9:26 AM
To: Horn, Hazel V; 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

Even if a facility does not require that techs be HT registered - the 
technicians themselves need go that extra step, take the initiative themselves 
and become HT certified.  If a portion of histologists don't recognize or care 
about the merit of certification how can we progress our profession to the 
status it deserves?   You have to believe in yourself in order for others to 
follow suit.   

With the exclusion of the front office; NSH is a volunteer organization that 
fights for our profession and it can be difficult to take on the big dogs of 
ASCP/CAP/CLIA.  I like to see this passion for our profession and if we keep 
this up eventually we will see results and the professional status we deserve. 

Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital  Clinics Neuromuscular Laboratory
600 Highland Avenue
Madison, WI  53792-5132 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, September 11, 2013 8:30 AM
To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

Histology does not get the respect or the recognition because histologists do 
not report results.  All of the complex testing we do is overlooked because the 
pathologists report the results.   CLIA standards are based on result 
reporting.   The CAP has looked the other way for years because pathologists 
would hire unregistered techs.  If pathologists would demand only registered 
techs half our battle would be won. 

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Wednesday, September 11, 2013 8:08 AM
To: 'Jennifer MacDonald'; Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

And the reason so many have been fighting for this for years. If a lab were 
looking for a Medical Technologist there would be no question.


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Tuesday, September 10, 2013 7:32 PM
To: Marcum, Pamela A
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

As long as we do not need certification, licensure and minium education 
requirements we will not be recognized as Laboratory Professionals.



From:   Marcum, Pamela A pamar...@uams.edu
To: 'joelle weaver' joellewea...@hotmail.com, 'Emily Sours'
talulahg...@gmail.com, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date:   09/10/2013 01:12 PM
Subject:RE: [Histonet] Unregistered HT
Sent by:histonet-boun...@lists.utsouthwestern.edu



I agree we have huge gray areas and not all histology schools are as good as 
they could be for what we are facing in Histology.  I keep harping on the fact

RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

Sorry Sarah some of us old timers do have BS and even MS degrees and still have 
to fight for every dime.  The whole field needs to be improved without any 
thought of how long someone has been in Histology.  We have the issue of people 
who have HTs being called HTLs due to the way the two registries were created.  

In the early 1980s the only thing you could get was the HT registry no matter 
how much education you had worked to get.  Then we got the HTL for management 
skills as it was stated and for several years HTs could grandfather in with no 
additional education above HS.  Then in about 1984 the HTL started to require 
the education level of BS.  Unfortunately, since the two registries were to 
some extend mixed by the overlap of needing or not needing a degree the whole 
field is confused about who is what.  I have been called a histologist before I 
took my HT (with a BS) and HTL although I did not bother to grandfather in.   
Yet I am called both and really don't care which I am called as long as I am 
respected.   An MS made no difference either way.

The real issue is most administration people and HR Departments don't know the 
difference between an HT and HTL and have no idea what to do.  I have seen HTs 
with a HS call themselves HTLs and no one knew the difference at the 
administration/HR levels.  (Some of those older people who started and may not 
even have a HS degree yet; are among the best histologist I have seen in 50 
years.  Some with BS and/or MS degrees were terrible.  Some MTs came over with 
CP pay and could be great or awful.)  Education is not answer.  The issue is 
straightening out who we are and what we do as either registry area. 

Here we had a salary survey and found most of the employers just paid the same 
for everything to avoid the issue or due to total misunderstanding of their 
even being a difference.  Now everyone is the same.  No one knew how to solve 
it to everyone's satisfaction and the histologist were not asked.  If you can 
say you have a BS you may get paid a little higher and only have an HT and be 
called an HTL.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Wednesday, September 11, 2013 1:30 PM
To: Blazek, Linda; Rene J Buesa; Clare Thornton; 'Emily Sours'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

I don't think that histotechs are disrespected at all.  There is always going 
to be that one person in any job that thinks they are better than you because 
of whatever reason.  HTs are not the highest paid people in the lab (or the 
lowest for that matter), and I think a lot of times people in management, PhD, 
or MD positions see us in a light that mirrors our salaries.  The fact of the 
matter is these days you have to basically have a college degree to become a 
HT, and hopefully as the old-timers who didn't have to have a degree retire 
out of the field we will be paid more according to the education that a lot of 
us paid so much money to obtain.

I still hold firm to the belief that you get what you give.  If you act like 
someone who should be disrespected you will be.  If you suggest new ideas for 
improvement, go over and beyond your pay grade, then eventually you will be 
paid for that financially and with respect. 

If I couldn't live like that I think that would be a very difficult and 
frustrating way to live every day.  Therefore, I choose to have a good attitude 
about being away from my family everyday (because I have to have a job), and do 
the best I can to make others have a reason to respect me.

Just my two cents...I will step down from my soapbox now =)

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna 
Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Wednesday, September 11, 2013 1:15 PM
To: Rene J Buesa; Clare Thornton; 'Emily Sours'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

Wow René, I didn't realize you had such a demeaning and disrespected career.  I 
have been in this profession for almost 40 years.  I have worked for some of 
the greatest pathologists you could ask for.  They have respected me, asked my 
opinions and shared a great deal of time at the microscope with me.  Maybe it 
has to do with attitude..

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, September 11, 2013 1:30 PM
To: Clare Thornton; 'Emily Sours'
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unregistered HT

The issue remains the WE cannot impose on others how we are viewed. No matter 
how many 

RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

Research is a different area and not controlled by CAP, CLIA and other hospital 
licensing or accreditation organizations.  We are bound by the rules of these 
organizations and while I agree with you to a point.  We do need minimums for 
training and registration by recognized licensing bodies when patient tissue is 
being processed for histological examination.  I am sure no one thinks of this 
often however; there are medical legal issues with insurance we have that do 
not apply for research.  It is also clear that registration does not mean we 
don't have registered people who are not as good as they should be for 
excellent patient care.

I have worked in research and while I would not ever say the hiring of 
non-registered people is a problem for research.  It is often a specialty area 
that requires knowing more than routine Histology.   I have done plastics in 
research that could not ever be used in routine Histology due to the time 
factors and in some cases limited use with staining applications, especially 
IHC for some procedures.  Many other areas in research require more specialized 
training than would be used in a routine area.  I would also add some really 
great techs are in many phases of research.  I know MT who work in Histology 
and are not registered as the MT BS, overrides the HT requirement for many 
institutions.  

Many factors must be considered for both research and routine Histology that 
cover far more than just hiring registered people in certain areas of the 
laboratory.

Pam Marcum 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, September 10, 2013 8:20 AM
To: joelle weaver
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unregistered HT

Do employers consider lab techs to be proficient enough? I've been doing ISH 
and immuno for 13 years, but I'm not certified as I do research.  Maybe there 
aren't a lot of lab techs out there? Just wondering since you might be missing 
someone awesome by hiring only certified people.

By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward.

-Chuck Palahniuk, Haunted


On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver joellewea...@hotmail.comwrote:

 All I have is a histology assistant description I put together. It 
 is mostly clerical, instrument up keep and other duties. My employer 
 does not hire uncertified techs- due to CLIA license, grossing, FISH  
 molecular duties ( high complexity). I hope to be a clinical site 
 eventually, and then they will have to complete their certification 
 within one year -if they are hired on.
 If you think that will help, I can send it on.




 Joelle Weaver MAOM, HTL (ASCP) QIHC

  Date: Mon, 9 Sep 2013 15:39:12 -0500
  From: dels...@gmail.com
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Unregistered HT
 
  Hi all,
 
  I'm wondering if anyone can share a job description for an 
  unregistered
 HT
  position. I also need to know if unregistered HT's are allowed to 
  cut frozen sections.  I know there is a limit to what they can do, 
  especially where IHC and special staining are concerned, but I 
  really don't know
 about
  frozens, since it's a lot like regular microtomy, except for the HE 
  staining of the slide. I also understand that facilities might have 
  different descriptions, but what I'm really looking for is what CAP
 allows
  them to do.
 
  Thanks for your help,
 
  Deloris Carter, HT(ASCP)
  SMMC
  Shawnee, KS
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message.
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RE: [Histonet] Unregistered HT

[Marcum, Pamela A]

I agree we have huge gray areas and not all histology schools are as good as 
they could be for what we are facing in Histology.  I keep harping on the fact 
that until we are recognized as Laboratory Professionals we will stay in this 
limbo.  The rules determining complex testing should be revisited to what is 
done in Histology Laboratories today and not what we did 30 or more years ago.  
The Clinical Laboratory is now so automated it is hard to find anyone in most 
areas who can even remember doing any manual testing.  The Micro lab is the 
closest to being as manual as areas of Histology.

I am in a small market and finding a registered Histologist is harder for us.  
I would love to have 8 to choose from and interview.

Pam Marcum

From: joelle weaver [mailto:joellewea...@hotmail.com]
Sent: Tuesday, September 10, 2013 2:59 PM
To: Marcum, Pamela A; 'Emily Sours'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

 Well I am  mostly clinical...but I think that organizations can set standards 
outside and beyond what CAP,CLIA etc stipulate. For the position I have now, I 
had to submit all my transcripts from high school up through masters in 
addition to  proof of my ASCP certification, IHC qualification, continuing 
education, and professional association activity. There is a lot of gray area 
out there. They seem to have not had trouble getting applicants though ( and I 
know this varies by market), there were over 8 candidates for an HT opening, 
which I thought was a pretty good turn out.


Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: pamar...@uams.edu
 To: talulahg...@gmail.com; joellewea...@hotmail.com
 CC: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Unregistered HT
 Date: Tue, 10 Sep 2013 13:53:26 +

 Research is a different area and not controlled by CAP, CLIA and other 
 hospital licensing or accreditation organizations. We are bound by the rules 
 of these organizations and while I agree with you to a point. We do need 
 minimums for training and registration by recognized licensing bodies when 
 patient tissue is being processed for histological examination. I am sure no 
 one thinks of this often however; there are medical legal issues with 
 insurance we have that do not apply for research. It is also clear that 
 registration does not mean we don't have registered people who are not as 
 good as they should be for excellent patient care.

 I have worked in research and while I would not ever say the hiring of 
 non-registered people is a problem for research. It is often a specialty area 
 that requires knowing more than routine Histology. I have done plastics in 
 research that could not ever be used in routine Histology due to the time 
 factors and in some cases limited use with staining applications, especially 
 IHC for some procedures. Many other areas in research require more 
 specialized training than would be used in a routine area. I would also add 
 some really great techs are in many phases of research. I know MT who work in 
 Histology and are not registered as the MT BS, overrides the HT requirement 
 for many institutions.

 Many factors must be considered for both research and routine Histology that 
 cover far more than just hiring registered people in certain areas of the 
 laboratory.

 Pam Marcum


 -Original Message-
 From: 
 histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
  
 [mailto:histonet-boun...@lists.utsouthwestern.edu]mailto:[mailto:histonet-boun...@lists.utsouthwestern.edu]
  On Behalf Of Emily Sours
 Sent: Tuesday, September 10, 2013 8:20 AM
 To: joelle weaver
 Cc: 
 histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Unregistered HT

 Do employers consider lab techs to be proficient enough? I've been doing ISH 
 and immuno for 13 years, but I'm not certified as I do research. Maybe there 
 aren't a lot of lab techs out there? Just wondering since you might be 
 missing someone awesome by hiring only certified people.

 By bitching and bitching and bitching, they could exhaust the drama of their 
 own horror stories. Grow bored. Only then could they accept a new story for 
 their lives. Move forward.

 -Chuck Palahniuk, Haunted


 On Mon, Sep 9, 2013 at 6:49 PM, joelle weaver 
 joellewea...@hotmail.commailto:joellewea...@hotmail.comwrote:

  All I have is a histology assistant description I put together. It
  is mostly clerical, instrument up keep and other duties. My employer
  does not hire uncertified techs- due to CLIA license, grossing, FISH 
  molecular duties ( high complexity). I hope to be a clinical site
  eventually, and then they will have to complete their certification
  within one year -if they are hired on.
  If you think that will help, I can send it on.
 
 
 
 
  Joelle Weaver MAOM, HTL (ASCP) QIHC
 
   Date: Mon, 9 Sep 2013 15:39:12 -0500
   From: dels...@gmail.commailto:dels...@gmail.com

RE: [Histonet] (no subject)

[Marcum, Pamela A]

The last answer will cover most paraffins used routinely and may cover 
microwave also.  Temperatures below 58C are often melt points however; they may 
not be a complete liquid or have slightly increased viscosity.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 8:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying 
for her boards:



I'm studying for my board exam and came across questions re: paraffin 
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or 
higher than that? What do we use?

This seems to me to be an odd question because it depends on the melting point 
of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have 
also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing 
something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

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RE: [Histonet] Uncertified Histotechs

[Marcum, Pamela A]

If you have pharmaceutical, some veterinary and research areas in universities 
or even some private labs are not as strict.  In the future we may all face the 
challenge of having a certification as a requirement and we should be ready it. 
 We have seen the field change and my only issue is getting ASCP, CLIA and NSH 
to assist us in getting recognition for what we do and schools to educate the 
next generations.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manfre, Philip
Sent: Monday, August 26, 2013 6:50 AM
To: Jon Hannasch; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Uncertified Histotechs

I would look to the pharmaceutical industry, or industry in general, if they 
are hiring.  I believe they are more willing to hire without certification than 
a hospital.


Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486



 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Hannasch
Sent: Thursday, August 22, 2013 7:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Uncertified Histotechs

Is getting a job as an uncertified histotech a thing of the past? I have a 
friend who has been a very skilled histotech for many years and they have been 
looking for a job for about a year now. Is this due to bad interviewing or a 
lack of certification? I'm curious to see if this has happened to other people. 
They have applied at hospitals and bigger labs such as Caris. Im not asking for 
a job lead for them I'm just more curious if certification has become a 
prerequisite now.
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RE: [Histonet] Uncertified Histotechs

[Marcum, Pamela A]

This will be difficult discussion for many of us who were OJT in the 60s to 
even into the 90s however; ASCP and states have now developed licensing 
regulations that require through CLIA  that almost prohibit the this track for 
registration.  The problem here is we have too few schools and hospitals 
willing to go that route.  We are now training people only on automated 
equipment at many sites and the fact they pass a test does not mean they 
troubleshoot problems from processing, embedding, sectioning and staining.  

We are only looking at people who have an HT or HTL at this point and it can be 
very difficult.  It is a decision based on the University and CLIA.

Pam


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, 
Beatrice
Sent: Friday, August 23, 2013 7:29 AM
To: joelle weaver; Jon Hannasch; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Uncertified Histotechs

While I understand the need for certification and continuing education, because 
of regulations it is very hard to even have your OJT's sit for their 
certification. Recently I interviewed candidates for an open position at my 
facility. One candidate in particular looked very good on paper. I brought this 
person in for an interview. Candidate was certified by CAP as a Histo- 
Technician. I always test their microtomy skills. Imagine my dismay when after 
more than I hour this person had not produced one decent slide. Needless to say 
the interview was pretty much over. Lesson here is that letters after one's  
name does not make a good Histo-Tech.

Beatrice Sullivan HT(ASCP)HTL  CLSP(NCA) Corporate Histology Supervisor 
Virtua,Voorhees

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Friday, August 23, 2013 8:00 AM
To: Jon Hannasch; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Uncertified Histotechs

It still seems to vary by market ( many factors including licensure in some 
states), and by organization based on my observations. But I believe that there 
is certainly a trend toward certification. At my organization they require 
certification for any consideration, and also education to meet CLIA.   But you 
are likely to get many different opinions on your question.




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: jon2038...@maricopa.edu
 Date: Thu, 22 Aug 2013 16:43:10 -0700
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Uncertified Histotechs
 
 Is getting a job as an uncertified histotech a thing of the past? I have a 
 friend who has been a very skilled histotech for many years and they have 
 been looking for a job for about a year now. Is this due to bad interviewing 
 or a lack of certification? I'm curious to see if this has happened to other 
 people. They have applied at hospitals and bigger labs such as Caris. Im not 
 asking for a job lead for them I'm just more curious if certification has 
 become a prerequisite now.
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 Histonet@lists.utsouthwestern.edu
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[Histonet] RE: Uncertified histotechs

[Marcum, Pamela A]

The interesting thing is the history of Histotechnican and Histotechnologist.  
We have probably all been called both if we have been in this field very long.  
Recently, I had a reason to find out how it actually came about and where the 
two titles came from originally and got so confused.  Prior to early 1980 we 
were all called by these two names and Histologist.  In the early 80s the ASCP 
developed a new management category for the registry.  The new category was 
Histotechnologist or HTL, with the idea that some of us could grandfather in 
over several years (if we had the experience) and then the category would 
require a four year or BS degree in order to sit for the test.  Now you are 
supposed to have a BS to take the HTL and be called a Histotechnologist.  HTs 
are called Histotechnicians according to ASCP.  

The problem is now we are so confused in many of the job categories and we are 
all still called by any of the three titles that getting paid more is hard or 
knowing what you are in the market.  HR departments and some managers are not 
sure what to call us or list jobs as in descriptions overall.  Recently here in 
Arkansas a market survey was done.  These issues really showed the differences. 
 No one really understood how to correct the titles as some of the employers 
only hired you if you had one of the titles and made no exception for which one 
and others just did not care as long as you were registered with ASCP.  The 
University made some slight difference due to education.  Due to the market 
confusion and ours as to what to call ourselves at times this was no help.  Now 
it is just you need an HT or HTL to apply.  We are not offering OJT due to 
some issues with several people we trained for a year or more, not following 
through with the registry.  It cost too much money and time with no return from 
trainees in these cases. 

I am an OJT from back in the 60s when it was the only route to get into 
Histology as we had even fewer schools and options.  Some of the best Histology 
people I know were OJT trained and some with MTs who never sat for the 
Histology registry are excellent.  We all know people who are trained now and 
even years ago that are not at the top of the field and are only interested in 
coming in, getting by and going home.  Just like every other field we have the 
full range of above average to no you can't ever do my tissue.  As long as we 
are fragmented this way it makes equitable pay and being recognized as a 
professional category by CAP a problem.  We do not do complex testing.  Really, 
have these people ever done IHC from titer to completion.  We can't give it to 
a pathologist unless we know it worked or why it did not and that is not 
complex.  

Pam Marcum
UAMS
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Friday, August 23, 2013 1:45 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Uncertified histotechs

It can be difficult to find employment without certification, however I have 
seen techs with certification who cannot perform.  It does depend on the 
facility and the environment.  Some say it is for liability purposes (in case 
there is a case that was mishandled) and some say for the regulations for 
health care law.  Either way it can be an asset, and I'm not just saying that 
because it's what I do.  
I did OJT, and then took the test because my supervisor said I had to.  The 
benefit was it has not been very difficult meeting the basic qualifications for 
jobs.  The only drawback is there is no standard amongst employers on position 
names (histotechnician for less than 5 yrs experience and histotechnologist for 
more than 5 yrs).  Certification and education can open doors for more complex 
testing in the future, especially if we (histotechs) want to remain in charge 
of our destiny's.  
So yes, the positions for non-certified histotechs are diminishing, depending 
on the location of the facility and the availability of techs in the area.  It 
also depends on whether or not there is a program in the area, if there is, the 
competition may be higher.  They can pay a new graduate less, even if they are 
certified, than a very experienced non-certified tech.  

I do agree that requesting a cutting test is a decent measure of baseline 
ability.  I did run into quite a few techs that were certified and cut the 
plastic on the cassette.  

Toysha N. Mayer, MBA, HT(ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
713-563.3481

--

Message: 5
Date: Thu, 22 Aug 2013 16:43:10 -0700
From: Jon Hannasch jon2038...@maricopa.edu
Subject: [Histonet] Uncertified Histotechs
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: 04d5279b-c201-4f0a-97c3-6d322a9fd...@maricopa.edu

RE: [Histonet] One step trichrome kits

[Marcum, Pamela A]

Try Polyscientific RD.  We use their kits for manual staining and they have 
been great.  

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori White
Sent: Wednesday, August 14, 2013 7:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] One step trichrome kits

Hi,

Can anybody recommend a one step trichrome  kit for manual staining?  We 
currently use the Light Green kit from Sigma and are not happy with the 
consistency of the reagents.

Thanks,

Lori W

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RE: [Histonet] RE: Two Patient Identifiers on slides

[Marcum, Pamela A]

We use the surgical case number and a minimum of the first 5 letters of the 
patients name on barcode labeled cassettes and then that code will be 
transferred to the slides as they are printed.  We have found that having two 
identifiers on every cassette and slide has saved us when an error was made 
with the cassette labeling or slide labeling process.  It has been a way to 
confirm or clear up an issue very quickly.  Whether it is required or not; at 
this point we will continue to use both identifiers for both the patient's 
safety and our ability to backtrack/clear issues of possible miss 
identification of a cassette or slide.  We are all human and mistakes happen so 
plan a way to limit the possibilities. 

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline
Sent: Wednesday, July 31, 2013 12:47 PM
To: Tom McNemar; Jean Wood; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two Patient Identifiers on slides

Just for clarification, patient first and last name? Thanks!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Wednesday, July 31, 2013 5:49 AM
To: 'Jean Wood'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Two Patient Identifiers on slides

Our cassettes are printed with the accession number, patient name, and a 
barcode that also contains the DOB.  When sectioning, this barcode is scanned 
at the microtome when the slides are cut.  Labels are printed, again at the 
microtome, and the slides are labeled.  The slide label contains the accession 
number, patient name, level number or special stain, the pathologist's 
initials, and the hospital address.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Wood
Sent: Tuesday, July 30, 2013 3:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Two Patient Identifiers on slides

Hello Histonetters,

   Recently we started utilizing a slide labeling component that is built 
into our AP Easy LIS system and has accession number, levels and patients first 
and last name when labels are printed out. Dymo does not have a chemically 
resistant label (we have a Dymo 450 printer) and we have been putting the 
labels on AFTER the slides are stained and cover slipped.

In the meantime, the HT is writing in pencil the accession # and levels on the 
slide which is then covered up with the permanent label after cover slipping. 
Our Lab Manager is worried that we are not compliant as we do not have two 
patient identifiers on throughout the whole process (she wants us to write 
patient names on slides in pencil (before
staining) and then cover that up with the pre-printed label after staining.

1. What is everyone else doing?
2. Have any of you found a chemically resistant label compatible with the Dymo 
labeler?


Jean Wood BS, HT
Fairchild Medical Center Pathology Dept.
Ph:530.841.6243 Fax:530.841.6232
jw...@fairchildmed.orgmailto:jw...@fairchildmed.org
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RE: [Histonet] Two Patient Identifiers on slides

[Marcum, Pamela A]

Due to legal issues, if we have a cassette mislabeled we request a cassette 
with the correct patient information immediately and re-embed the block.  
Biopsies are a concern as it is sometimes difficult to re-embed a small tissue, 
so we do our best.  We have had times when we cross out the barcode as we could 
not reliably re-embed and carefully make notes for any future legal problems 
(no matter how unlikely the case may be) to establish the identity clearly.  If 
the slides were cut and we cannot go back to the block due to tissue size or 
disease state we use the same procedure as cassettes and a paper label.  The 
real issue is training everyone to double check the name and case number with 
the paperwork as the cassettes and slides are printed and have no mistakes.  We 
are human and has been proven difficult at times.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline
Sent: Wednesday, July 31, 2013 1:14 PM
To: Joe W. Walker, Jr.; Michelle Moore; Jean Wood; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Two Patient Identifiers on slides

My understanding was the same as Joe's and at least to date, that is what we 
have presented during survey and it has been deemed acceptable.  However, we 
are accredited by TJC not CAP.  My question to those of you who are providing 2 
patient identifiers on blocks?  How do you make it fit?  If anyone could scan 
an image to caroline.pr...@uphs.upenn.edu I would greatly appreciate it!  As of 
now, we have the unique patient accession number and a barcode with the 
accession number as well.  We are in our survey window I would like to be sure 
we are compliant.  

One more question, if you label slides and blocks with a name and DOB from the 
requisition and find out during insurance validation that the name is 
misspelled or the DOB is inaccurate, how do you handle that?  Thanks for your 
help.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, 
Jr.
Sent: Tuesday, July 30, 2013 4:31 PM
To: Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Two Patient Identifiers on slides

I don't think this is technically correct according to the CAP.  The question 
in the most recent checklist states:

**REVISED** 07/11/2011

ANP.52000 Specimen ID   Phase II
The identity of every specimen and image, including blocks, slides, and 
electron micrographs, is maintained through each step in processing.

NOTE: Each block of tissue must be individually labeled with unique patient 
identifier(s), e.g.
accession number etched onto the block or embedded into it. Storage of 
unlabeled blocks in separate containers that are labeled with patient number or 
name does not meet this requirement.
Evidence of Compliance:

✓ Written procedure describing system for maintaining specimen identity

This question does not state that 2 identifiers must be on the slides and 
blocks, etc.  It states it must be a unique identifier throughout the process.  
A specimen accession number would technically suffice.  You can add additional 
identifiers if that helps in your process but they are not required.

I believe that this is often confused with the General lab question GEN.40491 
which requires that any specimen that is collected and submitted to the lab 
must have 2 unique identifiers on it.  Once the specimen is in the lab, the 
unique accession number would suffice.

Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I 
think it is a good idea as an extra precaution step.  We are fortunate to have 
a xylene resistant label and printer that allows us to label the slides prior 
to processing.

My two cents as a manager,

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewal...@rrmc.orgwww.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional…Vermont’s 1st Hospital to Achieve Both ANCC Magnet 
Recognition® and the Governor’s Award for Performance Excellence


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Moore
Sent: Tuesday, July 30, 2013 3:46 PM
To: Jean Wood; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Two Patient Identifiers on slides

We are writing path #, date of birth  patient initials as identifiers. Michelle



From: Jean Wood jw...@fairchildmed.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, July 30, 2013 2:37 PM
Subject: [Histonet] Two Patient Identifiers on slides


Hello Histonetters,

  Recently we started 

[Histonet] RE: Looking for input on tissue processors

[Marcum, Pamela A]

Hi Jim,

We have four Excelsiors here at UAMS in the Histology Lab.  We bought two to 
replace very old VIPs over 2 years ago and were very happy with the processing 
and the safety.  We have less exposure to fumes as well as ease of use.  As 
supervisor I am concerned about safety.  If I find something that will meet or 
exceed our needs while improving safety and reduce my reagent costs I will go 
for it.  The Excelsiors, especially since we now have replaced all except our 
bone marrow processor have reduced our cost by about 60% for processing.  We 
have had very few problems and they have been very responsive to us on any 
questions.  Training was excellent in Kalamazoo.   

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Monday, July 29, 2013 8:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Looking for input on tissue processors

We are evaluating tissue processors to replace one of our 1980's VIP.  So far 
we have looked at the Thermofisher STP420, Leica ASP6025, VIP6, and we may look 
at theThermofisher Excelsior ES.
I would be interested in the opinions of other customers which processor they 
would go with and why.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



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RE: [Histonet] Shandon Excelsior Tissue Processor

[Marcum, Pamela A]

Since we have some power issues here and 4 Excelsiors (no problems with them on 
 this issue) we have UPS units on all of our computerized equipment.  We did 
this due to issues in other areas of the lab and decided it was safer than 
taking a chance and being down.  We bought Powervar and I think the suggested 
units were around $2,000 to $3,000 each with one unit serving two processors.  
Other sites in Little Rock who have some of the same power issues have had 
problems.

Pam Marcum
UAMS


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Tony Henwood (SCHN) 
[tony.henw...@health.nsw.gov.au]
Sent: Wednesday, June 12, 2013 6:40 PM
To: 'Velez, Vanessa'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Shandon Excelsior Tissue Processor

Vanessa,

We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector.

Cost under $1,500 Australian.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager  Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa
Sent: Thursday, 13 June 2013 2:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Shandon Excelsior Tissue Processor

Does any one having problems with the Excelsior Tissue Processor power board 
repeatedly after power outage?  This has happened to me three times in the last 
five years even though it has backup batteries.
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[Histonet] Does anyone sell or have Bielchowsky slides for sale?

[Marcum, Pamela A]

Please let me know if you can help on this one.

Pam Marcum
UAMS

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[Histonet] RE: Does anyone sell or have Bielchowsky slides for sale?

[Marcum, Pamela A]

Sorry, I was not clear.  I need the control slides if someone offers them.

-Original Message-
From: Bernice Frederick [mailto:b-freder...@northwestern.edu] 
Sent: Monday, March 18, 2013 2:15 PM
To: Marcum, Pamela A
Subject: RE: Does anyone sell or have Bielchowsky slides for sale?

Are you looking for control slides? I see Newcomer and Rowley have the staining 
kits.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Monday, March 18, 2013 1:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Does anyone sell or have Bielchowsky slides for sale?

Please let me know if you can help on this one.

Pam Marcum
UAMS

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RE: [Histonet] streaks

[Marcum, Pamela A]

They were using AccuEdge here for years and we changed to the Thermo MX35 
Premier 3 years ago with no issues.  In fact everyone was very upset for about 
a month and then they did not want to change back.  So, it really depends on 
the knife and as I was told after using them for month they could not tell a 
difference.  If you went back with a pack of AccuEdge, they were just as happy 
to stay with the Premier blade.  

I started with the steel knives almost 47 years ago and have seen so many 
changes and improvements in knives that I have learned to try something new and 
see rather than buy by brand or habit only.  Sometimes it works and sometimes 
it does not however; you never know till you give it a good try.

Pam Marcum
UAMS 
Histology Supervisor 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Wednesday, February 27, 2013 2:05 AM
To: fourfonn...@yahoo.com; cthorn...@dahlchase.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] streaks

Many vendors are pushing different blades...but the only blade that is superior 
is the Accu-edge. If you are using any other you will very likely get inferior 
sections. That is 30 years of experience using Accu-edge blades always trying 
others and always going back to the best.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Tuesday, February 26, 2013 12:34 PM
To: cthorn...@dahlchase.com; Histonet
Subject: [Histonet] streaks

Hi Claire,
 
I may be grasping at straws here, but I went to an NSH workshop once where this 
very question was posed.  You won't believe what the cause was.  They had a bad 
lot of blades.  The whole lot was nicked, so changing to a new knife or a new 
box of knives did not help the problem.  Changing the lot did the trick.  I 
don't know if this is your problem or not, but I thought it was very 
interesting.
 
Sheila HT (ASCP)
Knoxville, TN
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