*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk ***
I'm currently working with a glycoprotein and am having great difficulty deglycosylating it for crystallisation. I can completely remove the sugars very easily when the protein is denatured (not much good when you're wanting to crystalise it), but seems completely resistant to treatment with endo H or pngase F when done under native conditions. Any hints on getting this to work would be greatly appreciated. ################################## Dr Paul A. McEwan Protein Crystallography Group Centre for Biomolecular Science Clifton Boulevard University of Nottingham Nottingham NG7 2RD Tel: 0115 8468009 http://www.nottingham.ac.uk/~pazxtal ################################## This message has been checked for viruses but the contents of an attachment may still contain software viruses, which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
