Fisher Scientific makes 22 inch square plastic cover slips, which are
labeled "Fisherbrand Unbreakable
Cover Slips".
I and my colleagues have used them for over 15 years instead of siliconized
glass coverslips.
Just dip each one in ethanol to clean before use, dry with an air blower,
and use it
in my experience using silane with PAGE plates, rain-x is much better (at least
in that case).
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Goldman,
Adrian
Gesendet: Donnerstag, 31. Januar 2019 17:43
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Is there any
Yeah I used to use Fisher Scientific square plastic cover slips. Now I am using
Sigma 22x22mm (Z36,590-4, 100 each Lot10B084279) smaller sized ones. These
plastic ones are much more durable than the glass ones and are very easy to be
handled. They work very well for both hanging drops or for
For the last 150,000 years we've been using the Hampton THICK siliconized
coverslips - I'm told they're one of the many crystallization tricks/gizmos
that Alan D'Arcy invented. We quite often recycle them - and I've never
seen any problems. The thick ones are very easy to use because you can jam
Looking for:
Solid-state NMR research technician (PhD/MSc)
University of Groningen, Zernike Institute for Advanced Materials
Van der Wel SSNMR group
— Deadline Feb. 14th —
The Van der Wel solid-state NMR group at the University of Groningen
(Netherlands) is looking for applicants with a
Dear Colleagues,
The Diffraction Group in the Neutron Scattering Division (NSD) at Oak Ridge
National Laboratory (ORNL) has an immediate opening for a Postdoctoral Research
Associate in macromolecular crystallography and/or structural biology to
investigate the structure and function of
Dear all, I am considering to buy a new laptop and would like to install
the latest ubuntu version and have 3D glasses. Following the discussions
in the lasts years, this issue seems to be not trivial. Which setup of
laptop and 3D glasses will work plug and play?
And what about VR. When is
Awesome solution :-) I am sure, users will appreciate it. And I don't
need stereo personally :-)
Jan
On 1/31/19 9:26 AM, Hughes, Jon wrote:
> ...just open pymol, go to display - stereo mode - cross-eye then click
> stereo. it might need a little practice and it makes one look even
> sillier than
Or do sitting drop, it's much easier all round. Frank
Sent from tiny silly touch screen
From: Minmin Yu
Sent: Thursday, 31 January 2019 21:42
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips
for
Agree. Nowadays I hardly do hanging drops and crystal clear tapes are good to seal the sitting drop plates too. I mainly use the plastic cover slips to set the cryo drops on for crystal harvesting. They can be put on any plates and the drops are good to be viewed under the microscope.
Dear all,
I had an HP Z620 that was dual boot Kubuntu 14.03 and Win 10. Coot was working
in stereo mode without any problem for a year or so. But after one of their
major updates Coot crashed as soon as the built-in IR emitter of my Asus VG278H
turned on, as described Pedro and Jan.
My personal
huge thanks everyone.. what a response. All good now
:)
-Original Message-
From: Pedro Matias [mailto:mat...@itqb.unl.pt]
Sent: den 31 januari 2019 11:46
To: Dean Derbyshire ; CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] FW: [ccp4bb] old data - headers
Hi Dean,
I reckon that it is an
maybe a silly question it there a data base or other way to tell what detector
was used to collect historic data. Image header isn't hugely helpful
ESRF is all I know for the source but I'd like to know what the detector was at
the time... - I'm talking 2005-2010
Dean Derbyshire
Hi Dean
Usually there is a serial number buried somewhere in the header - many are text
headers though some have TIFF format binary headers. Often a timestamp as well
though this is less common
From there biosync may help e.g.
Hi Dean
I'd ask someone who was involved in writing the data processing programs at
that time to have a quick shufti at the images! There are a few who read the
BB...
Harry
--
Dr Harry Powell
> On 31 Jan 2019, at 09:38, Dean Derbyshire wrote:
>
> maybe a silly question it there a data base
Hi
This looks like it was on beamline 7.2 (which had a fixed wavelength of 1.488Å,
according to an article written by Liz Duke - see
https://www.ccp4.ac.uk/newsletters/newsletter37/11_beamline14.html); I can't
remember if detector 421 was a Q4 or a Q4R, but (again, according to the same
A long time ago, before siliconized coverslips became commercially available,
we used to siliconize coverslips ourselves. It is not really that much work and
unsiliconized cover slips should be very cheap. If you wish, I could try to
find back the protocol.
Best,
Herman
Von: CCP4 bulletin
Hi,
I believe that one can put a 50-100uL drop of fresh SigmaCote (in a tube cap)
with the glass pieces (surface well exposed), sealed in a dedicated (because
the container will be coated too) container (air-tight lunch boxes). After a
while the SigmaCote vapor should react with the glass
Dear all,
does anyone happen to know exactly which Xe flash lamp is (or better: was)
being used in the discontinued Nanodrop-1000?
Thanks for your help!
Bärbel
--
Bärbel Blaum, PhD
Inthera Bioscience AG
Einsiedlerstrasse 34
CH-8820 Waedenswil
Switzerland
E-Mail:
Hi Rajnandani,
The cheaper DIY protocol that Herman referred to, is actually still in use by
us. Use a siliconizing solution (e.g. Serva 35130.02 silicone solution in
isopropanol). Put about 20-30 microliters on the coverslips, preferrably with a
repeating pipette and in a fumehood. Then
It helps to know the filename extension. Opening the image with adxv may
also provide clues as to the detector type.
Às 09:42 de 31/01/2019, graeme.win...@diamond.ac.uk escreveu:
> Hi Dean
>
> Usually there is a serial number buried somewhere in the header - many are
> text headers though some
thanks all. I recon I have the ESRF data sorted.. but Daresbury.. am I right in
assuming MARCCD or are we going back as far as image plate?
here is the image header
Harry what do you think
HEADER_BYTES= 512;
DIM=2;
BYTE_ORDER=little_endian;
TYPE=unsigned_short;
PIXEL_SIZE=0.08160;
BIN=none;
Hi Dean,
I reckon that it is an ADSC Quantum 4 or 4R CCD detector. If you open
the images with mosflm or adxv you should see the characteristic 2x2
tile pattern.
Note how the header format is similar to the one you posted from the ESRF.
Pedro
Às 10:21 de 31/01/2019, Dean Derbyshire escreveu:
>
Hi all,
The problem with COOT stereo and Windows 10 is that the COOT binary is
not compatible with the newer OpenGL drivers or somesuch in Windows 10.
So, COOT runs fine in Windows 10 but crashes if you enter hardware
stereo mode. However, PyMOL works fine in stereo in Windows 10.
According to
Dear All
I am a PhD student who requires lots of coverslips (!!) for setting up
hanging drop crystallization. The company sells it for a huge amount. Also
there is a wide monetary difference between a normal siliconized coverslip
and a 22mm siliconized circle coverslips. We tried to search for an
...just open pymol, go to display - stereo mode - cross-eye then click stereo.
it might need a little practice and it makes one look even sillier than one
does usually, but it works, costs nothing, needs no updates and i'm told that
it's even good for your eye muscles!
best,
jon
Von: CCP4
Dear All,
We are pleased to announce a 2.5 day practical workshop on sample
preparation for cryo-EM single particle data collection jointly
organised by the ESRF, IBS and the EMBL Grenoble. This is the second of
a series of practical hands-on workshops started in 2018. This workshop
is aimed
Title: Postdoctoral position University of Oxford (UK)
Weatherall Institute of Molecular Medicine (University of Oxford, UK) offers an
outstanding opportunity for a highly motivated and talented Postdoctoral
Scientist specialized in structural biology to join a research project on a
>>
Deadline 1 Feb, 2019
https://www.imperial.ac.uk/jobs/description/NAT00344/research-associate-position-chemical-glycobiology-francis-crick-institute/
Job Summary:
Up to two Postdoctoral Research Associate positions are available in the
Chemical Glycobiology Laboratory hosted by the
Hi All,
There are 3 open positions in my group in the Max Planck Institute for the
Structure and Dynamics of Matter (MPSD) in Hamburg. All positions involve
developing and implementing algorithms for new diffractive imaging
techniques. You can find more information about the institute at
Deal all,*
*
I would like to bring your attention to
*32^nd European Crystallographic Meeting@ University of Vienna, 18^th to
23^th August 2019*
The 32^nd European Crystallographic Meeting, ECM32, will take place in
Vienna, Austria, from 18^th to 23^rd of August 2019. The organizers of
the
Hello Harry
I used SRS 7.2 and 9.6 at a wide variety of monochromatic wavelengths for
resonant scattering (AD) studies. But I can imagine high intensity application
PX measurements were made at those specific wavelengths which you mention.
Greetings from Novosibirsk,
John
Emeritus Professor of
Hi Dean, Harry,
Station 14.1 was also run at 1.488A, partly to maintain the lower energy
capacity that disappeared with the closure of 7.2. We are talking here about
the last period of SRS Daresbury, in 2005 when staffing and equipment were in
'high flux'. Marccd never went on 14.1, it was
I know of Rain-X being used.
V. Nagarajan
On Thu, Jan 31, 2019 at 1:17 AM wrote:
> A long time ago, before siliconized coverslips became commercially
> available, we used to siliconize coverslips ourselves. It is not really
> that much work and unsiliconized cover slips should be very cheap. If
When I was a graduate student, about 150,000 years ago, we took regular
coverslips and doused them in ?silane to make siliconised ones. You then let
them sit in a rack to dry. It was a bit tedious but not horrendously so.
After a while, I stopped doing it altogether, because IMHO it didn’t
Long back we used Prosil solution (I think, 1% solution) for siliconization.
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Goldman,
Adrian
Sent: Thursday, January 31, 2019 8:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Is
Yes, that was it! I just couldn’t remember the name.
> On 31 Jan 2019, at 17:04, Mathews, Irimpan I.
> <2add487f8799-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Long back we used Prosil solution (I think, 1% solution) for siliconization.
> Mathews
>
>
> -Original Message-
> From:
yes, rain-x is excellent (also for PAGE gel plates). a little bottle costs
almost nothing and will last you a lifetime.
best
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von
Nagarajan V
Gesendet: Donnerstag, 31. Januar 2019 16:14
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re:
plastic.
Plastic cover slips are no good for UV or polarization, but they are way better
than glass if you happen to want to try in-situ diffraction.
(https://doi.org/10.1107/S002188981254)
If you can't afford commercial ones, then you can always cut up some inkjet
transparency film
Dear Colleague,
We are pleased to announce the 7th International School on Biological Crystallization (ISBC2019), to be held in Granada (Spain) during May 26th to 31st, 2019 (http://www.isbcgranada.org/).
The School will provide five days of
We used to use dichlorodimethylsilane in toluene to siliconize both
coverslips and the special glass capillaries for crystal mounting.
I don't have the protocol we used anymore, but the one listed on
protocolpedia sounds familiar.
41 matches
Mail list logo