Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Fisher Scientific makes 22 inch square plastic cover slips, which are labeled "Fisherbrand Unbreakable Cover Slips". I and my colleagues have used them for over 15 years instead of siliconized glass coverslips. Just dip each one in ethanol to clean before use, dry with an air blower, and use it

[ccp4bb] AW: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

in my experience using silane with PAGE plates, rain-x is much better (at least in that case). jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Goldman, Adrian Gesendet: Donnerstag, 31. Januar 2019 17:43 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Is there any

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Yeah I used to use Fisher Scientific square plastic cover slips. Now I am using Sigma 22x22mm (Z36,590-4, 100 each Lot10B084279) smaller sized ones. These plastic ones are much more durable than the glass ones and are very easy to be handled. They work very well for both hanging drops or for

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

For the last 150,000 years we've been using the Hampton THICK siliconized coverslips - I'm told they're one of the many crystallization tricks/gizmos that Alan D'Arcy invented. We quite often recycle them - and I've never seen any problems. The thick ones are very easy to use because you can jam

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Re: [ccp4bb] 3D stereo and (pymol) Win 10

Dear all, I am considering to buy a new laptop and would like to install the latest ubuntu version and have 3D glasses. Following the discussions in the lasts years, this issue seems to be not trivial. Which setup of laptop and 3D glasses will work plug and play? And what about VR. When is

Re: [ccp4bb] AW: [ccp4bb] 3D stereo and pymol

Awesome solution :-) I am sure, users will appreciate it. And I don't need stereo personally :-) Jan On 1/31/19 9:26 AM, Hughes, Jon wrote: > ...just open pymol, go to display - stereo mode - cross-eye then click > stereo. it might need a little practice and it makes one look even > sillier than

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Or do sitting drop, it's much easier all round. Frank Sent from tiny silly touch screen From: Minmin Yu Sent: Thursday, 31 January 2019 21:42 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

‎Agree. Nowadays I hardly do hanging drops and crystal clear tapes are good to seal the sitting drop plates too. I mainly use the plastic cover slips to set the cryo drops on for crystal harvesting. They can be put on any plates and the drops are good to be viewed under the microscope. 

Re: [ccp4bb] 3D stereo and (pymol) Win 10

Dear all, I had an HP Z620 that was dual boot Kubuntu 14.03 and Win 10. Coot was working in stereo mode without any problem for a year or so. But after one of their major updates Coot crashed as soon as the built-in IR emitter of my Asus VG278H turned on, as described Pedro and Jan. My personal

Re: [ccp4bb] FW: [ccp4bb] old data - headers

huge thanks everyone.. what a response. All good now :) -Original Message- From: Pedro Matias [mailto:mat...@itqb.unl.pt] Sent: den 31 januari 2019 11:46 To: Dean Derbyshire ; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] FW: [ccp4bb] old data - headers Hi Dean, I reckon that it is an

[ccp4bb] old data

maybe a silly question it there a data base or other way to tell what detector was used to collect historic data. Image header isn't hugely helpful ESRF is all I know for the source but I'd like to know what the detector was at the time... - I'm talking 2005-2010 Dean Derbyshire

Re: [ccp4bb] old data

Hi Dean Usually there is a serial number buried somewhere in the header - many are text headers though some have TIFF format binary headers. Often a timestamp as well though this is less common From there biosync may help e.g.

Re: [ccp4bb] old data

Hi Dean I'd ask someone who was involved in writing the data processing programs at that time to have a quick shufti at the images! There are a few who read the BB... Harry -- Dr Harry Powell > On 31 Jan 2019, at 09:38, Dean Derbyshire wrote: > > maybe a silly question it there a data base

Re: [ccp4bb] FW: [ccp4bb] old data - headers

Hi This looks like it was on beamline 7.2 (which had a fixed wavelength of 1.488Å, according to an article written by Liz Duke - see https://www.ccp4.ac.uk/newsletters/newsletter37/11_beamline14.html); I can't remember if detector 421 was a Q4 or a Q4R, but (again, according to the same

[ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

A long time ago, before siliconized coverslips became commercially available, we used to siliconize coverslips ourselves. It is not really that much work and unsiliconized cover slips should be very cheap. If you wish, I could try to find back the protocol. Best, Herman Von: CCP4 bulletin

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Hi, I believe that one can put a 50-100uL drop of fresh SigmaCote (in a tube cap) with the glass pieces (surface well exposed), sealed in a dedicated (because the container will be coated too) container (air-tight lunch boxes). After a while the SigmaCote vapor should react with the glass

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Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Hi Rajnandani, The cheaper DIY protocol that Herman referred to, is actually still in use by us. Use a siliconizing solution (e.g. Serva 35130.02 silicone solution in isopropanol). Put about 20-30 microliters on the coverslips, preferrably with a repeating pipette and in a fumehood. Then

Re: [ccp4bb] old data

It helps to know the filename extension. Opening the image with adxv may also provide clues as to the detector type. Às 09:42 de 31/01/2019, graeme.win...@diamond.ac.uk escreveu: > Hi Dean > > Usually there is a serial number buried somewhere in the header - many are > text headers though some

[ccp4bb] FW: [ccp4bb] old data - headers

thanks all. I recon I have the ESRF data sorted.. but Daresbury.. am I right in assuming MARCCD or are we going back as far as image plate? here is the image header Harry what do you think HEADER_BYTES= 512; DIM=2; BYTE_ORDER=little_endian; TYPE=unsigned_short; PIXEL_SIZE=0.08160; BIN=none;

Re: [ccp4bb] FW: [ccp4bb] old data - headers

Hi Dean, I reckon that it is an ADSC Quantum 4 or 4R CCD detector. If you open the images with mosflm or adxv you should see the characteristic 2x2 tile pattern. Note how the header format is similar to the one you posted from the ESRF. Pedro Às 10:21 de 31/01/2019, Dean Derbyshire escreveu: >

Re: [ccp4bb] 3D stereo and pymol

Hi all, The problem with COOT stereo and Windows 10 is that the COOT binary is not compatible with the newer OpenGL drivers or somesuch in Windows 10. So, COOT runs fine in Windows 10 but crashes if you enter hardware stereo mode. However, PyMOL works fine in stereo in Windows 10. According to

[ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an

[ccp4bb] AW: [ccp4bb] 3D stereo and pymol

...just open pymol, go to display - stereo mode - cross-eye then click stereo. it might need a little practice and it makes one look even sillier than one does usually, but it works, costs nothing, needs no updates and i'm told that it's even good for your eye muscles! best, jon Von: CCP4

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Re: [ccp4bb] FW: [ccp4bb] old data - headers

Hello Harry I used SRS 7.2 and 9.6 at a wide variety of monochromatic wavelengths for resonant scattering (AD) studies. But I can imagine high intensity application PX measurements were made at those specific wavelengths which you mention. Greetings from Novosibirsk, John Emeritus Professor of

Re: [ccp4bb] old data - headers

Hi Dean, Harry, Station 14.1 was also run at 1.488A, partly to maintain the lower energy capacity that disappeared with the closure of 7.2. We are talking here about the last period of SRS Daresbury, in 2005 when staffing and equipment were in 'high flux'. Marccd never went on 14.1, it was

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

I know of Rain-X being used. V. Nagarajan On Thu, Jan 31, 2019 at 1:17 AM wrote: > A long time ago, before siliconized coverslips became commercially > available, we used to siliconize coverslips ourselves. It is not really > that much work and unsiliconized cover slips should be very cheap. If

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

When I was a graduate student, about 150,000 years ago, we took regular coverslips and doused them in ?silane to make siliconised ones. You then let them sit in a rack to dry. It was a bit tedious but not horrendously so. After a while, I stopped doing it altogether, because IMHO it didn’t

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Long back we used Prosil solution (I think, 1% solution) for siliconization. Mathews -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Goldman, Adrian Sent: Thursday, January 31, 2019 8:43 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Is

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Yes, that was it! I just couldn’t remember the name. > On 31 Jan 2019, at 17:04, Mathews, Irimpan I. > <2add487f8799-dmarc-requ...@jiscmail.ac.uk> wrote: > > Long back we used Prosil solution (I think, 1% solution) for siliconization. > Mathews > > > -Original Message- > From:

[ccp4bb] AW: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

yes, rain-x is excellent (also for PAGE gel plates). a little bottle costs almost nothing and will last you a lifetime. best jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Nagarajan V Gesendet: Donnerstag, 31. Januar 2019 16:14 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re:

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

plastic. Plastic cover slips are no good for UV or polarization, but they are way better than glass if you happen to want to try in-situ diffraction. (https://doi.org/10.1107/S002188981254) If you can't afford commercial ones, then you can always cut up some inkjet transparency film

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Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

We used to use dichlorodimethylsilane in toluene to siliconize both coverslips and the special glass capillaries for crystal mounting. I don't have the protocol we used anymore, but the one listed on protocolpedia sounds familiar.