d profound brain things inside my head,
> I have decided to thank you for bringing peace to our home.
>Julian, King of Lemurs
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
ustified residue names, however pdb may use
> only one of them.
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
but rather you want to use it as a tool
to verify the location of a ligand binding site.
Thanks!
F
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
lliam Scott wrote:
> she asked were a few questions about a specific computer (HP Z210 8 GB with
> a low end Quadro Nvidia 400 512 MB) running "any Linux", and a specific
> computer (IMAC 4 GB 2.5 GHz with AMD Radeon HD 6750M 512 MB)
----------
ime will tell if the
drives in 2010 were any better than then drives from 2007. I doubt it though.
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
I already started with an ellipse... should I extend with a rectangle down the
shaft of the beamstop?(this is FRAME.cbf after integration)
<>
Thanks!
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
ed by some algorithm by those programs during integration, or is it up to
the scaling/merging (by SCALA for example) step to deal with them?
Thanks!
F
<>
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
messy density maps.
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
t for the solvent mask for the
other? How's this done? (a direct input of the better xtal1 mask to xtal 2
gives 'inconsistent cell info').
Thanks!
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
there some formatting error I'm not seeing?
Thanks!
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
ation but could be a good starting point.
>
> Determining the transformation may be tricker - I'd try placing pseudo-atoms
> at similar features in both maps and using lsqkab to get the transformation
> matrix; but this approach would probably need tuning to get a good alignment.
&
data for improvement of a group
II intron structure. Acta Crystallogr D Biol Crystallogr (2010) vol. 66 (Pt 9)
pp. 988-1000
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
est not everyone
> is really on board. Again, I am sure this has to be done, but consensus
> in this area is equally important.
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
e a hypothesis that the true symmetry is different. I could
> test my hypothesis by reprocessing the data were it available.
and thank you for providing yours .
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
re is a binding, but when i take the same thing
> to set drops, only DNA crystals. Kindly suggest me, what could be done.
>
>
> Thanks & Regards,
> Umar Farook.S
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
On the other hand, shooting a lower resolution crystal may get you the
conformation of the disordered domain.
Surprising at first thought, but was true in p97/VCP from the Brunger Lab.
F
On Dec 7, 2011, at 11:47 PM, atul kumar wrote:
> Dear all
>
> I have crytals which diffract up
and processing" in Warwick there will be live streaming. This is
>> at the link http://extrplay.dl.ac.uk/ kicking off with the what's new session
>> at 9:00am GMT. The main event starts at 11:00am GMT.
>>
>> The full program is at
>> http://www.cse.scitech.
ting at low resolutions is another.
>
> Thanks for pointing this out to me - I'll have to check out the details of
> how phenix handles it, and give it a try.
>
> Pete
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
dide) for 5-10 seconds and collect the anomalous
signal at home. If it kills your crystals, move to more toxic things...
>
> Thanks in advance,
>
> Federica
>
Cheers,
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
AD/SAD nomenclature I favor explicitly stating whether
dispersive/anomalous/isomorphous differences (and what heavy atoms for each )
were used in phasing. Aren't analyzing the differences (independent of
source) the important bit anyway?
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
08889), and/or its affiliates Direct contact information
>> for affiliates is available at
>> http://www.merck.com/contact/contacts.html) that may be confidential,
>> proprietary copyrighted and/or legally privileged. It is intended solely
>> for the use of the individual or entity named on this message. If you are
>> not the intended recipient, and have received this message in error,
>> please notify us immediately by reply e-mail and then delete it from
>> your system.
>>
>>
>>
>> --
>>
>> ARKA CHAKRABORTY
>> CAS in Crystallography and Biophysics
>> University of Madras
>> Chennai,India
>>
>
>
>
>
> --
>
> ARKA CHAKRABORTY
> CAS in Crystallography and Biophysics
> University of Madras
> Chennai,India
>
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
ed to be the protein with mass spec.
>
> Any suggestions to improve diffraction would be welcome.
>
> Thanking you in advance.
>
> Theresa
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
e cracked crystals diffract? Do you see the analogue?
F
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
ooks so nice. To be disappointed by big
> crystals is often not a surprise to me and if you have not tried small
> crystals from the same batch well then you missed 50% of your chances to
> solve s structure with the first light the crystals saw.
>
> Jürgen
>
>
-
Acta Cryst. (1997). D53, 734-737[ doi:10.1107/S0907444997007233 ]
The Ultimate Wavelength for Protein Crystallography?
I. Polikarpov, A. Teplyakov and G. Oliva
http://scripts.iucr.org/cgi-bin/paper?gr0657
may give some insights.
To the OP, have you solved the structure? In some cases, s
D/year). Or the user can go rogue
and 'Ctrl-Click' install any application.
http://www.apple.com/macosx/mountain-lion/security.html
Personally I'll probably choose Mac App Store and identified developers. (I
imagine this will be the default).
-----
t; You could sign your own software
> (for free) and then distribute your public key to the community, in case
> you want to do something similar.
[FUD] OS X won't trust those keys, only the ones that come from apple [/FUD]
F
---------
Francis E
ize the lack of peaks on the self rotation maps).
Any help would be appreciated.
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
d crystals in the same drop.
>
> Thanks for any input.
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
e. I also see lot of
>> unanswerable density in the map where lot of waters were placed. Model fits
>> to the map like a low resolution data with most of side chains don't have
>> best density.
>>
>> I was not expecting such a sudden drop in the R/Rfree and
http://skuld.bmsc.washington.edu/scatter/AS_form.html
Maybe useful to you.
However, I would advise to do a fluorescence scan over the range given in the
graph and then use chooch to provide the precise energies for your peak and
inflection.
If you have a large crystal don't expose all of it
o missing large number of reflections.
Perhaps a screen shot of one of the images where this is happening would be
helpful. So we can judge the quality of the diffraction / whether the space
group is correct, etc.
F
---------
Francis E. Reyes M.Sc.
215 UCB
he anomalous signal if heavy atom is there?
>
> thanks in advance for help and suggestions..
>
>
>
>
>
> regards
>
> Shanti Pal Gangwar
>
>
>
>
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
I'm now preparing for the flood of 'unsubscribe ccp4bb' requests
On Apr 2, 2012, at 9:15 AM, Bernhard Rupp (Hofkristallrat a.D.) wrote:
> Guys,
>
> http://www.youtube.com/watch?v=CobZuaPMQHw
>
> second 9 in this 22 sec video
>
>
> -Original Message-
> From: CCP4 bulletin boa
It seems that deposition of map coefficients is a good idea. Does someone have
an mtz2cif that can handle this?
Thanks!
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
this region because of disorder or bad model
phases?
Would love people's thoughts on this one,
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
A weighted 2Fo-Fc map (which I believe is the default for most
crystallographic refinement packages).
F
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
P21.. You sure about this space group? (very high confidences for space group
and laue group in pointless?)
F
On Apr 19, 2012, at 12:20 AM, LISA wrote:
> Hi all,
>
> I am trying to solve one structure by molecular replacement with phaser in
> CCP4. This a complex of a multi-domain domains
For the RNA I recommend Eric Westhof's Assemble.
F
On May 23, 2012, at 9:32 AM, jens j birktoft wrote:
> Hi everybody,
>
> Does anyone know of a (non-commercial) software that are suitable for
> modeling DNA/RNA structures. Coot is great, however I am looking something
> that allows more f
Hi all
Are there any online/offline 3D crystal lattice builders to explore
symmetry relationships in the unit cell given say a space group and
unit cell parameters? I need one for an educational opportunity.
Thanks!
F
-
Francis E. Reyes M.Sc
CCP4'ers,
Are 9 datasets the maximum for an mtz file? or a single run of cad?
The manual (http://www.ccp4.ac.uk/html/cad.html) seems to suggest that
9 is the limit per cad run but not for a given mtz.
F
-
Francis E. Reyes M.Sc.
21
.4462 -
-0.1051 -0.9808 0.1642
shift -
invert -
-108.0451 47.655 -17.5654
end
Can someone supply me with the correct matrix to input into DM (and
what convention it is?)
Thanks
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
Oops
It pays to view the pdbset log as it gives the right matrix.
F
On Dec 1, 2010, at 10:41 AM, Francis E Reyes wrote:
Hi all
I'm trying to figure out rotation matricies for ncs averaging in DM.
Except the conventions have got me confused.
The following input script for p
:)
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
ingen Biomolecular Sciences and Biotechnology Institute
& Zernike Institute for Advanced Materials
University of Groningen
Nijenborgh 4, 9747 AG
Groningen, The Netherlands
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver
delling it with water refines perfectly with
no indication of positive difference density even at 2.0 sigma cut
off. Should I modelled this density as water or as Mg2+. Your
opinions are appreciated.
JL
-------------
Francis E. Reyes M.Sc.
215 UCB
University
on as it's available. Our apologies for the
inconvenience.
Best wishes,
Ronan
Ronan Keegan
CCP4 Group
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
Hi all
Is there a walkthru/tutorial for processing inverse beam images with
imosflm/scala? Googling a few things didn't get me anywhere.
Thanks!
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mi
before)
F
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
at am I doing wrong? Please help me with
suggestions
Many thanks
Careina
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
age or documentation that explains
how 'Scor' is computed?
[2] Based on the results above, it seems that P 21 21 21 is the
correct s.g.?
[3] Why the failure?
Thanks!
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at B
cific position with respect to your protein.
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
Can someone return their full path of the location of multilog (used
for browsing dmmulti output)?
I can't find it in my ccp4-6.1.13 installed from Mr Scott's distrib.
Thanks!
F
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
e resolution of the data is about 3.1A).
The unit cell is:
142.0500 142.0500 137.9200 90. 90. 120.
P6422.
Any ideas appreciated,
Thanks!
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
Never had problems with evaporation (and this is in the relatively dry
climate of Denver, CO, especially in the winter when the relative
humidity is in the low 20%).
Using the Thermo Scientific Nanodrop 2000c.
We use it also as a prerequisite for ITC, which can be very sensitive
to proper
them to be black on the
walls. I'm looking for a supplier / catalog number of such plates.
To save bb spam, I'll take replies privately.
Thanks!
F
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
scala to scale) ?
Am I interpreting this correctly?
Thanks!
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
Depending on the corectness of the (experimental or model) phases, I've seen
Co at the Ir peak and Ir at the Co peak. Use both to cross validate your model
(when you get to it) and calculate experimental phases using both (dual SAD or
2wl MAD for each heavy atom).
So no it's not
s!
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
the names are not compatible. I'm trying to build an RNA
> molecule by the way.
>
> Any suggestions?
>
> Eric
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
ng a rigid body or very restrained (
secondary structure | reference structure | etc ) refinement of the coordinates
for helping the fit.
Thanks!
F
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
chains/tails. You do this and subsequent refinement still does not produce the
expected density for the chains.
or
[3] Your solution here.
If this situation has been discussed before, please let me know .
F
---------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
What is the space group? Is there unmodeled density? Are all residues
in your construct built? What is the refinement protocol?
FR
On Dec 18, 2009, at 6:47 AM, james09 pruza
wrote:
Dear All,
I am trying to solve a 2.55 A resolution data set. The R-factor is
around 24% while Free-R is
Hi all
I have data integrated and scaled to P21 via denzo/scalepack. I'm concerned
about
the workflow to obtain the alternate indexing arrangement (h,k,l) ->
(h,-k,-h-l).
I was thinking .sca ( not specifying NO MERGE) -> .mtz -> reindex but the
documentation for reindex says all my DANO col
+1 phenix FTW
FR
On Jan 13, 2010, at 3:28 PM, Peter Zwart wrote:
try using
phenix.reflection_file_converter data.sca
--change_of_basis="h,-k,-h-l" --sca=reindex.sca
or something like that
HTH
Peter
2010/1/13 Francis E Reyes :
Hi all
I have data integrated and scaled
Or know of any references besides Declercq and Evrard, 2001 ? I'd love
to hear from you. Please PM me.
Cheers
FR
-
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 2
How does one calculate a simulated annealing omit map in cases of non
crystallographic symmetry? The omit map is being used to identify the
presence and configuration of a ligand and surrounding residues. The
omit region therefore will contain the ligand and residues within
hydrogen bonding
Hi All
I'm having trouble with the following ...
title [No title given]
operator -1/2*h+1/2*k-l, 1/2*h-1/2*k-l, -1/2*h-1/2*k
labin I(+)=I_Unspecified(+) SIGI(+)=SIGI_Unspecified(+)
I(-)=I_Unspecified(-) SIGI(-)=SIGI_Unspecified(-)
end
I keep getting symmetry operator error.
Thanks
FR
-
I like the fact that MVE's plastic shipping cases have a round top, so
Fedex can't ship the container upside down.
However, I like Taylor-Wharton's wide mouth 91mm (CXR100) vs MVE's
70mm(SC 4/2V). Unfortunately Taylor-Wharton's recommended shippers
are square and could be sent upside down.
Hi all
I'm in search of literature *detailing* low resolution [4-5A] phasing
protocols. Heck I'll take detailed thesis chapters (since they tend to be more
detailed than pubs anyway). If you ribosome people are on this board I'd love
to hear from you.
[sarcasm]Searching for 'grasping at stra
Is this a cause for concern? FOM's are over 0.5 and Phasing Power is over 2.0.
Thanks
FR
-
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5
Hi all
I have a solved structure that crystallizes as a trimer to a reasonable
R/Rfree, but I'm trying to rationalize the peaks in my self rotation. The space
group is P212121, calculating my self rotations from 50-3A, integration radius
of 22 (the radius of my molecule is about 44). I can see
Hi all
I'd like to add a phase error to my PHIB's and FOM's (experimental phases) that
increases linearly with higher resolution.. it's akin to taking good phases and
making them bad. Any approaches on how this can be done?
Thanks
FR
-
Francis Reyes M
Hi all
I've been playing around with charge flipping for macromolecular
substructure determination with pretty promising results. I'm
particularly attracted to the fact that it solves structures in P1,
with no space group assumptions and curious how it would handle some
of the pseudosymme
Sorry a late comer to this thread but the OP mentioned "tweaking the
error model" in HKL2000. I have heard this before.What's the validity
in this? Does it actually help or does it only help the integration
numbers but you'll pay for it during refinement?
FR
On Jun 23, 2010, at 8:25 AM, "Zh
to be able to accommodate 35 cm (wide) by 26-30cm (height) plates.
Please reply privately.
F
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
d you
never are able to completely get rid of Y. How does this affect the
analysis of determining the derivative's affinity by ITC?
References or personal experience is appreciated!
F
-----
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Bo
Hi all
I'm interested in the scenario where crystals were screened at home
and gave lousy (say < 8-10A) but when illuminated with synchrotron
radiation gave reasonable diffraction ( > 3A) ? Why the discrepancy?
Thanks
F
---------
Francis E.
c=144, alpha=119, beta=119, gamma=90),
-
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
re heating than one backsoaked.
Thanks
F
-------------
Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
Hello all,
I believe I have a P41 perfectly twinned as a P41 21 2. I'm using the
detwin_perfect.inp in CNS in every round of refinement to detwin the
original P41 data as I update the single molecule in my asymmetric
unit (so far).
[1] Should I be able to find the second molecule (from re
Ironically as this stereo discussion ensues, it seems NVidia is
pushing 3d glasses once again (http://tech.slashdot.org/tech/08/09/17/1530202.shtml
) . Those 120Hz LCD's are welcome reprieve from the bulky SGI
monitors in our x-ray core now.
More on topic, however, as a 'newer' generation (
On Nov 18, 2008, at 11:41 AM, Anna S Gardberg wrote:
3. Eddie Snell made the point that most crystallographic software
cannot take advantage of multiple processors yet. Still, as some
people pointed, it is often convenient to try several refinement
options at the same time, so there would
On Nov 18, 2008, at 9:57 AM, Brian Mark wrote:
Hi all,
With all the talk about Mac OS X, I've not heard much mention about
OS X Server and networking Macs together. Is anyone using the 10.5
server and LDAP to centrally house user directories on a RAID
connected to a Mac server for exampl
Non CCP4 related!
If you have experience screening for light sensitive compounds when
xtals are obtained, can you send me a personal e-mail describing any
tips,tricks, pitfalls, pointers?
thanks
FR
-
Francis Reyes M.Sc.
215 UCB
University of Col
Does such a thing exist? A 24-well microplate configuration where in
substitution of glass cover slips, you have a roll of tape templated
such that there are circular areas where you can add your protein
where there is no adhesive, but there is adhesive everywhere else?
This may be a nightm
Since this was of discussion on this board a week or so ago, I figure
it has relevancy.
http://store.nvidia.com/DRHM/servlet/ControllerServlet?Action=DisplayProductDetailsPage&SiteID=nvidia&Locale=en_US&Env=BASE&productID=111286400&utm_source=newsletter&utm_medium=email&utm_campaign=store_Jan09
There we have it then! Port everything to DirectX and use Vista ! ;)
*shudder*
Not to slam M$, but I run vista x64 at home and the hardware support
is awful (manufacturers have yet to distinguish between driver support
for 32 bit or 64 bit i.e. Vista capable on the box may mean only 32
bit
Not sure if it's been mentioned, but I personally use EnzymeX(http://mekentosj.com/enzymex/
) .
Also, I find their PDF library organizer Papers (http://mekentosj.com/papers/
) to be exceptional.
Cheers
FR
On Jan 28, 2009, at 1:47 AM, Darren Hart wrote:
Hello,
After several years of offering
I have trouble with visualizing things in three dimensions so I'm
trying to figure out the relationship between two cross rotation
functions (given as theta1, theta2, theta3).
Is there a program or webapp that'll tell me whether two rotation
solutions are related by a 180/90/60/whathaveyo
It seems like this space group will be the death of me.
I'm working on a structure in SG P41212 one molecule per asu that was
solved with experimental SAD phases. The resolution is to 2.5 and the
refinement is stuck at an R/Rfree of 30 and 33 with bonds rmsd of
0.011 and angles of 1.597 .
Jürgen
In the case you have an overwhelming amount of images, why not instead
just setup an automatically generated RSS feed (on a server that you
or maybe the ccp4 project or wiki will host) that contains them?
There is an RSS screensaver already built into leopard, I'm sure there
is on
I think this was posted earlier, but the geometry constraints for the
cartoon mode is a little tight for a residue of my protein. What's the
setting to relax them so it'll draw a continuous tube instead of
leaving it blank?
Thanks
FR
-
Francis R
All
Single isomorphous replacement with anomalous scattering uses two
datasets that are isomorphous while using the anomalous signal in one
(here scattering and dispersion are synonymous?). How does SIRAS use
the anomalous scattering signal that's different than a single
wavelength anomal
I have a mtz from Autosol/resolve that has the following columns:
OVERALL FILE STATISTICS for resolution range 0.002 - 0.261
===
Col SortMinMaxNum % Mean Mean
Resolution Type Column
num order Missing complete abs.
Why do i keep getting an error when running molrep with locked
rotation functions?
Open failed: Unit: 8, File: /tmp/francisreyes/
qv_9_molrep_crossrot_alo.dat (logical: /tmp/francisreyes/
qv_9_molrep_crossrot_alo.dat)
MOLREP(ccp4): Open failed: File: /tmp/francisreyes/
qv_9_molrep_crossr
I am experimenting with GLRF and am having trouble calculating the
locked self rotation function for a protein of known structure. The
protein has a 3 fold NCS axis that is not parallel to a
crystallographic axis. I'm at the step of specifying the local
symmetry elements for the locked sel
Are you talking about chunks of acrylamide? Or perhaps trace molecules?
As for chunks, we normally pass it through a 0.2 um filter before
setting up trays.
FR
On Apr 1, 2009, at 4:48 PM, vanessa delfosse wrote:
Dear all,
I'm currently trying to crystallize a 70 nt RNA and I would like to
Despite setting a dataset name in the dialog box, i get the following
in the log file.
$TEXT:Warning: $$ comment $$
WARNING: PROJECTNAME not assigned
$$
$TEXT:Warning: $$ comment $$
WARNING: CRYSTALNAME not assigned
$$
$TEXT:Warning: $$ comment $$
WARNING: DATASETNAME not assigne
All
It seems I have a case where I have 5595 reflections but my protein is
about 102 residues. With a mean atom / residue * 4 parameters for
each atom I get about 7833 parameters. So it seems that I have a
observation : parameter ratio < 1. There is only 1 molecular per asu
so there's no
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