Re: [ccp4bb] Reproducing crystals.

? Please help me. Thanks in advance. -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England

Re: [ccp4bb] crystallizing a complex that's sensitive to ionic strength

, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

[ccp4bb] Fab purification and crystallization

Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp

Re: [ccp4bb] turn granular to crystal

, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of rui Sent: 25 August

Re: [ccp4bb] Scaling up from an Intelliplate to Linbro Plate

://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034

Re: [ccp4bb] control of nucleation

Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of zq deng Sent: 06 May 2010 09:04 To: CCP4BB@JISCMAIL.AC.UK

Re: [ccp4bb] freezing crystals grown in isopropanol condition

to deal with isopropanol evaporation during mounting? Many thanks and best wishes, Chris -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148

Re: [ccp4bb] 24-well sitting-drop plates

protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http

[ccp4bb] Vapor diffusion calculator

Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jacob Keller Sent

Re: [ccp4bb] anaerobic crystallization

patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] stabilizing solution

?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480

Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

! There's more info at http://www.douglas.co.uk/rat_des.htm Patrick --  patr...@douglas.co.uk    Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk/  Tel: 44 (0) 148-864-9090    US toll

Re: [ccp4bb] How to improve crystal which is twinning?

, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board

Re: [ccp4bb] microbatch vs hanging drop

Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

, please join our bulletin board at http://groups.google.com/group/oryx_group/members_invite?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148

Re: [ccp4bb] Query on program for creating random crystallization screen

, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf

Re: [ccp4bb] about microbatch

, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jiamu Du Sent: 26

Re: [ccp4bb] isopropanol as a precipitant

know if anyone wants samples. Patrick -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1

[ccp4bb] Fwd: [ccp4bb] crystallisation robot

One thing that people often overlook is that quite a lot of protein can be lost by denaturation on the surface of the drop. This is more significant for smaller drops. Two suggestions: (1) increase the proportion of protein in the - technical term - teeny drop to say two thirds and (2) cover the

Re: [ccp4bb] Help with reducing crystal mosaicity

25.7%. You can see the data at www.douglas.co.uk/top14.htm I thought this information could be useful if you want to replace some of the MPD with another precipitant (or cryoprotectant). Best wishes Patrick Shaw Stewart --  [EMAIL PROTECTED] Douglas Instruments Ltd.  DouglasHouse

[ccp4bb] Demonstration and support specialist

. No salesmen please. Please reply to the address below, or e-mail. Patrick Shaw Stewart --  [EMAIL PROTECTED] Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://douglas.co.uk or http

Re: [ccp4bb] Question about glove boxes for protein crystallization

Hi Mathews One of our customers, Bret Dillard at the University of Georgia, has had great success with one of our robots in a Bactron X anaerobic chamber. In this case, Bret mainly used the microbatch-under-oil method, which works very well for anaerobic work: (1) the oil protects the sample

Re: [ccp4bb] Main topic of the day: Protein crystallization

Ronaldo I have a database of crystallization conditions extracted from the PDB. I was able to parse the data to extract the concentration of protein in around 900 cases. The lowest 9 protein concentrations were as follows: PDB ID TYPEDATEProtein conc, mg/ml 1EYMISOMERASE.

Re: [ccp4bb] crystal friendly solvents that are useful for dissolving hydrophobic small molecules?

) microbatch-under-oil. The oil acts as a reservoir that contains excess small molecule that (you hope) will be fed into the crystals. Patrick Shaw Stewart -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock

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