[galaxy-dev] Galaxy Proxy by an External Apache Server

2012-01-04 Thread Derrick LIN
Hi guys, I am trying to setup a new galaxy for my institute. The galaxy itself runs fine in our private network. Now I am trying to configure the proxy so that it can be accessed publicly. The official guide here http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy It seems to assume both gala

Re: [galaxy-dev] Status on importing BAM file into Library does not update

2012-01-04 Thread Ryan Golhar
> > On Wed, Jan 4, 2012 at 5:17 PM, Ryan Golhar > wrote: > >> I'm adding Data Libraries to my local galaxy instance. I'm doing this by >> importing directories that contain bam and bai files. I see the bam/bai >> files get added on the admin page and the Message is "This job is running". >> qst

Re: [galaxy-dev] Empty files when importing with no copy from NFS source

2012-01-04 Thread Carlos Borroto
On Wed, Jan 4, 2012 at 12:59 PM, Carlos Borroto wrote: > Hi, > > While trying to import some fastq files to a data library, using > "Upload directory of files" and "Link to files without copying into > Galaxy", I end with empty datasets. > > My data files are accessed through NFS and I'm using the

Re: [galaxy-dev] Status on importing BAM file into Library does not update

2012-01-04 Thread Ryan Golhar
On Wed, Jan 4, 2012 at 5:17 PM, Ryan Golhar wrote: > I'm adding Data Libraries to my local galaxy instance. I'm doing this by > importing directories that contain bam and bai files. I see the bam/bai > files get added on the admin page and the Message is "This job is running". > qstat shows the

[galaxy-dev] Status on importing BAM file into Library does not update

2012-01-04 Thread Ryan Golhar
I'm adding Data Libraries to my local galaxy instance. I'm doing this by importing directories that contain bam and bai files. I see the bam/bai files get added on the admin page and the Message is "This job is running". qstat shows the job run and complete. I checked my runner0.log and it regi

Re: [galaxy-dev] galaxy command line

2012-01-04 Thread Nate Coraor
On Jan 4, 2012, at 2:34 PM, Langhorst, Brad wrote: > Ryan: > > I didn't understand Usha's question the same way you did. I agree that it's > useful to run workflows in a more automated way. > > I have not run workflows using the Galaxy API yet, but I did see some > documentation on it and plan

Re: [galaxy-dev] Galaxy Server on Bio-Linux

2012-01-04 Thread Carlos Borroto
On Tue, Jan 3, 2012 at 11:36 AM, Nate Coraor wrote: > On Dec 20, 2011, at 1:09 PM, Bicak, Mesude wrote: > >> Dear Galaxy Developers, >> >> We work in Professor Dawn Field's group (Molecular Evolution and >> Bioinformatics Research Group) at the NERC Environmental Bioinformatics >> Centre (NEBC)

[galaxy-dev] How to import data from a non-local source using SCP?

2012-01-04 Thread Ryan
I have a cluster (cluster A) set up with Galaxy. Our sequencing data gets mapped to hg19, then the resulting BAM files are placed on a SAN connected to a different cluster (cluster B) that cluster A does not have NFS access to. We cannot install an FTP server on cluster B either. The only way to

Re: [galaxy-dev] galaxy command line

2012-01-04 Thread Langhorst, Brad
Ryan: I didn't understand Usha's question the same way you did. I agree that it's useful to run workflows in a more automated way. I have not run workflows using the Galaxy API yet, but I did see some documentation on it and plan to try it soon. http://wiki.g2.bx.psu.edu/Learn/API/Examples Ma

Re: [galaxy-dev] galaxy command line

2012-01-04 Thread Carlos Borroto
On Wed, Jan 4, 2012 at 2:25 PM, Ryan wrote: > On Wed, Jan 4, 2012 at 12:04 PM, Langhorst, Brad wrote: >> >> Usha: >> >> Galaxy is essentially a wrapper around other command line tools. >> So, while you could probably extract some of its pieces to run on the >> command line, I don't know why you w

Re: [galaxy-dev] galaxy command line

2012-01-04 Thread Ryan
On Wed, Jan 4, 2012 at 12:04 PM, Langhorst, Brad wrote: > Usha: > > Galaxy is essentially a wrapper around other command line tools. > So, while you could probably extract some of its pieces to run on the > command line, I don't know why you would want to. > > I would argue it would be benefic

[galaxy-dev] Empty files when importing with no copy from NFS source

2012-01-04 Thread Carlos Borroto
Hi, While trying to import some fastq files to a data library, using "Upload directory of files" and "Link to files without copying into Galaxy", I end with empty datasets. My data files are accessed through NFS and I'm using the recommended option -noac: 10.2.90.89:/projects on /local/projects t

Re: [galaxy-dev] galaxy command line

2012-01-04 Thread Langhorst, Brad
Usha: Galaxy is essentially a wrapper around other command line tools. So, while you could probably extract some of its pieces to run on the command line, I don't know why you would want to. brad -- Brad Langhorst New England Biolabs langho...@neb.com From: Usha Reddy mailto:usha.reddy...@gma

[galaxy-dev] load balancing and proxy settings with Apache

2012-01-04 Thread Ryan
I'm at the final stage of deploying our Galaxy instance. I'm implementing the proxy server and load balancing with 1 job runner, and 5 web runners using Apache. I'm following the guide on: http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Web%20Application%20Scaling All the Galaxy universe_wsg

Re: [galaxy-dev] Best practices with data on clusters

2012-01-04 Thread Nate Coraor
On Jan 4, 2012, at 6:03 AM, Cittaro Davide wrote: > Hi Nate, > > On Jan 3, 2012, at 10:15 PM, Nate Coraor wrote: >> >> That said, if you have a lot interim steps that produce large data that then >> get merged via some process back to final outputs, it absolutely makes sense >> to use local di

Re: [galaxy-dev] [galaxy-user] (OperationalError) unable to open database file

2012-01-04 Thread Nate Coraor
On Jan 4, 2012, at 4:40 AM, Cai Shaojiang wrote: > Thanks, Nate, > > I now switched to MySQL, and the problem disappeared. Thanks. > > The manual highly recommend postgresql, is there any critical point to > use it, instead of mysql? I am just more familiar with mysql. Thanks. PostgreSQL suppor

Re: [galaxy-dev] Problem "password authentication failed for user "postgres" "

2012-01-04 Thread Nate Coraor
On Jan 3, 2012, at 8:35 PM, Huayan Gao wrote: > Thanks Nate! > > I also want to do a mirror site of Galaxy Server in asia, but found the > instruction is not so clear/detailed. Do we have a detailed one? Hi Huayan, Please keep replies on the mailing list for the benefit and collaboration of

[galaxy-dev] How to define comment character for output files

2012-01-04 Thread Sabry Razick
Hello, 1. How to define a character used at the beginning of a comment for ourput file. i.e. if "#" is used at the start of the comment Galaxy recognises this, but what if I want to use some other character. is it possible to define this inside ? 2. How does the "Number of commen

Re: [galaxy-dev] Problem with uploading SMALL file to Galaxy on EC2 and get "client intended to send too large body"

2012-01-04 Thread Dannon Baker
Thon, This is a problem with the default client_max_body_size option in nginx being set far too small in the nginx.conf on the cloud AMI. It'll be fixed with our next AMI update, but you could also SSH in to your instance, edit the nginx.conf to change the client_max_body_size to something mor

Re: [galaxy-dev] tool_type="data_source_async"

2012-01-04 Thread Daniel Blankenberg
Hi Matt, The asynchronous protocol should be working fine. Can you confirm that your flow is similar to: - User starts at Galaxy, gets sent to external resource with 'GALAXY_URL' parameter. - User browses external site and selects options, sends data to Galaxy by using the GALAXY_URL parameter

Re: [galaxy-dev] Galaxy tool's error report

2012-01-04 Thread Peter Cock
On Wed, Jan 4, 2012 at 5:24 AM, Timothy Wu <2hug...@gmail.com> wrote: > Hi, > > I'm executing a R script via Python's os.system() (using Rscript executable > which allow executing R script in the command line). This script makes use > of library that will attempt to load up Tcl/Tk interface. Though

Re: [galaxy-dev] tool_type="data_source_async"

2012-01-04 Thread Matt Vincent
Does this functionality exist? If so, how do we get it working? Sorry to bump! On 1/3/12 8:50 AM, "Matt Vincent" wrote: ost recent version of Galaxy. ___ Please keep all replies on the list by using "reply all" in your mail client. To man

Re: [galaxy-dev] Best practices with data on clusters

2012-01-04 Thread Cittaro Davide
Hi Nate, On Jan 3, 2012, at 10:15 PM, Nate Coraor wrote: That said, if you have a lot interim steps that produce large data that then get merged via some process back to final outputs, it absolutely makes sense to use local disk for those steps (assuming local disk is large enough - another pr

[galaxy-dev] Fwd: tool restrict access

2012-01-04 Thread Ivan Merelli
Hi, in order to restrict the access of a tool to logged users I'm trying to use Cheetah for editing the xml config file. I was wondering if a solution like the one below should work. Although no errors are reported while loading the tool it does not perform the check on the email address and a