Re: [ccp4bb] Off topic: Nobel prize stats

Forgot to credit the Nobel Prize website :The Nobel Prize in Chemistry 1946nobelprize.org-BWL To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1

Re: [ccp4bb] Off topic: Nobel prize stats

The Nobel Prize in Chemistry 1946 James Batcheller Sumner “for his discovery that enzymes can be crystallized” Prize share: 1/2 John Howard Northrop and Wendell Meredith Stanley "for their preparation of enzymes and virus proteins in a pure form" Prize share 1/2 jointly Thought it might be w

Re: [ccp4bb] Off topic - fluorescence plate reader in cold room or cold box

Dear Markus, I've had to solve this exact issue in the past. Putting a 'simple' (nothing is simple these days, everything has some kind of AI or other evil gnomes in it) spectrofluorimeter into the cold room *should* be OK. Caveat #1: I don't know if this will work with your specific device Cavea

Re: [ccp4bb] Off topic - fluorescence plate reader in cold room or cold box

I think you are right, in the final equilibrium all the moisture would condense on the (evaporator?) coils, and there should be provision for them to drip into a reservoir outside. However each time the door opens and humid air is admitted, there will be condensation everywhere. If you leave th

[ccp4bb] Off topic - fluorescence plate reader in cold room or cold box

Dear All, apologies for the off-topic question. I am facing the problem of running enzyme activity assays at low temperature (close to 4C would be ideal) in a fluorescence plate reader. Before I get labeled in our department as "the one who voids warranties" and destroys our beloved plate reader, I

Re: [ccp4bb] Off topic: Nobel prize stats

Thank you all for useful links, slides and thoughtful comments. I completely agree with Mark's comment that it is difficult to quote a single number. Precisely for the reasons he mentioned. The link to PDB, https://pdb101.rcsb.org/learn/other-resources/structural-biology-and-nobel-prizes seems the

Re: [ccp4bb] Off topic: Nobel prize stats

x27; ; 'CCP4BB@JISCMAIL.AC.UK' Asunto: RE: [ccp4bb] Off topic: Nobel prize stats This is not the same, but it may help... https://www.xtal.iqf.csic.es/Cristalografia/index-en.html Martin _ Dr. Martin Martinez-Ripoll Research Professor Emeritus mart

Re: [ccp4bb] Off topic: Nobel prize stats

You may want to add Kavli Prize laureates as well. -Lisa Keefe IMCA-CAT > On Jul 22, 2024, at 8:59 PM, Joel Sussman wrote: > >  > 22-Jul-2024 22:00 > Dear Nukri, > Please see: > https://proteopedia.org/w/Nobel_Prizes_for_3D_Molecular_Structure > best regards > Joel > > --

Re: [ccp4bb] Off topic: Nobel prize stats

22-Jul-2024 22:00 Dear Nukri, Please see: https://proteopedia.org/w/Nobel_Prizes_for_3D_Molecular_Structure best regards Joel Prof. Joel L. Sussman joel.suss...@weizmann.ac.il Dept. of

Re: [ccp4bb] Off topic: Nobel prize stats

I prepared this slide for a course (it only goes back to 1962, and some more recent awards should be added; but it’s a start). I looked at the PDB website and I think it would be a stretch to say that some of these were awarded for structural biology; the PDB puts in a link that relates the aw

Re: [ccp4bb] Off topic: Nobel prize stats

Nice, AI failed yet again to produce a comprehensive list of awardees. Some of the names match up with your link but it is very incomplete. Jürgen > On Jul 22, 2024, at 2:47 PM, William Scott > <2844d921eb97-dmarc-requ...@jiscmail.ac.uk> wrote: > > https://pdb101.rcsb.org/learn/other-reso

Re: [ccp4bb] Off topic: Nobel prize stats

https://pdb101.rcsb.org/learn/other-resources/structural-biology-and-nobel-prizes > On Jul 22, 2024, at 11:41 AM, Nukri Sanishvili wrote: > > Dear All, > Does anyone know the number of Nobel Prizes awarded for macromolecular > structures? > This would be a very effective way to explain the imp

[ccp4bb] Off topic: Nobel prize stats

Dear All, Does anyone know the number of Nobel Prizes awarded for macromolecular structures? This would be a very effective way to explain the importance of structural biology to the uninitiated. I suppose I could look at the whole list of laureates and figure out what was given for what, but if so

Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

Have you tried Guanidinium chloride? Jürgen > On Jul 3, 2024, at 1:22 PM, Daniele de Sanctis wrote: > > Hi Flavio > > I’d suggest to contact Regenfix > https://www.regenfix.eu/ > > Usually they give you back a column better than the original one completed > with an experimental calibration

Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

Hi Flavio I’d suggest to contact Regenfix https://www.regenfix.eu/ Usually they give you back a column better than the original one completed with an experimental calibration curve. Cheers Daniele On Wed 3 Jul 2024 at 18:56, Nicholas Clark < b2b1c7e93c2d-dmarc-requ...@jiscmail.ac.uk> wro

Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

Hi Flavio, I’d recommend reaching out to Cytiva directly ( scientificsupport...@cytiva.com). We had an issue with oxidized metal on our S200 and they were able to provide useful suggestions (plus side is, you know what they suggest won’t ruin the resin). Best, Nick Clark Nicholas D. Clark, PhD

Re: [ccp4bb] Off-topic: UCSF Chimera Download

I realised i have its chimeraX, not the old chimera. Sorry. O. On 18. Jun 2024, at 18:21, Kovtun, Oleksiy mailto:oleksiy.kov...@mpinat.mpg.de>> wrote: I dug out a MacOS dmg file, too. I will DM you a link. O. On 18. Jun 2024, at 18:09, Nicholas Clark mailto:ndcla...@buffalo.edu>> wrote: Tha

Re: [ccp4bb] Off-topic: UCSF Chimera Download

I dug out a MacOS dmg file, too. I will DM you a link. O. On 18. Jun 2024, at 18:09, Nicholas Clark mailto:ndcla...@buffalo.edu>> wrote: Thanks for the responses. I’ve made multiple attempts over the last few days which is why I reached out. Maybe it’s just high traffic and I’ll have to keep

Re: [ccp4bb] Off-topic: UCSF Chimera Download

Hi Nick, their web site goes offline from time to time. If you are on linux, I can share recent binaries. Best, Oleksiy On 18. Jun 2024, at 17:53, Nicholas Clark > wrote: I’m trying to navig

Re: [ccp4bb] Off-topic: UCSF Chimera Download

upgrades. Hope this helps. Andy From: CCP4 bulletin board on behalf of Nicholas Clark <b2b1c7e93c2d-dmarc-requ...@jiscmail.ac.uk> Sent: 18 June 2024 17:09 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Off-topic: UCSF Chimera Download External Sende

Re: [ccp4bb] Off-topic: UCSF Chimera Download

Thanks for the responses. I’ve made multiple attempts over the last few days which is why I reached out. Maybe it’s just high traffic and I’ll have to keep trying. Oleksiy, thanks for the offer but I’m on MacOS. Best, Nick On Tue, Jun 18, 2024 at 12:07 PM Kovtun, Oleksiy < oleksiy.kov...@mpina

[ccp4bb] Off-topic: UCSF Chimera Download

I’m trying to navigate to the download page for UCSF Chimera but it does not seem to be working. Does anyone know of an alternative location? Thanks, Nick Clark To unsubscribe from the CCP4BB list, click the following link

Re: [ccp4bb] [off topic] Recovering pET expression plasmid from BL21 strain

Hi Javier, For a few years (during an industry job) we regularly cloned directly into BL21 (we made our own high competency cells) and confirmed via expression before plasmid sequencing. Only after sequencing did we transform into a cloning strain for miniprep and “long term storage”. We didn’t s

Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavable his-tag at the c-ter

recommended by another. Others may have further suggestions for Flavio. *From: *CCP4 bulletin board on behalf of Karla J. F. Satchell *Date: *Friday, February 16, 2024 at 7:13 AM *To: *CCP4BB@JISCMAIL.AC.UK *Subject: *Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for

Re: [ccp4bb] [off topic] Recovering pET expression plasmid from BL21 strain

This should all go fine. You can maxi- or mini-prep the plasmid DNA from the expression strain and transform it back into a cloning strain for sequencing, etc. Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent from Proton Mail mobile Original Message On 19 Feb 2024, 0

[ccp4bb] [off topic] Recovering pET expression plasmid from BL21 strain

Dear all, I'm sure this issue comes up very often, but for the first time in our lab we need to recover a pET-type expression plasmid from a BL21-like E. coli strain (NEB's T7 Express). I know a RecA+ strain is not suitable for plasmid production, but the basic plan is to grow and mini-prep the cel

Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavable his-tag at the c-ter

Date: Friday, February 16, 2024 at 7:13 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavable his-tag at the c-ter Hi everyone— Thought I might chime in. This technology was developed in my lab based on our studies of

Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavable his-tag at the c-ter

Satchell From: CCP4 bulletin board on behalf of Srivastava, Dhiraj <bda2be8a675a-dmarc-requ...@jiscmail.ac.uk> Date: Friday, February 16, 2024 at 6:42 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavab

Re: [ccp4bb] [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavable his-tag at the c-ter

sequence, it may leave either no extra residues or only one or two residues. Dhiraj From: CCP4 bulletin board on behalf of Flavio Di Pisa Sent: Friday, February 16, 2024 3:33 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [External] [ccp4bb] Off topic : plasmid expression

[ccp4bb] Off topic : plasmid expression vector for E.Coli with a cleavable his-tag at the c-ter

Dear community, I'm looking for a commercial expression vector for E.Coli with a cleavable his-tag at the c-ter. Anyone can help me? Thank you in advance, Flavio. To unsubscribe from the CCP4BB list, click the followin

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

ffer conditions. Good luck! Irwin From: CCP4 bulletin board on behalf of Hughes, Jonathan Sent: 02 November 2023 09:17:30 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] AW: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage (this is from a different jon) if a chelator real

[ccp4bb] AW: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

23 19:53 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage I have always performed TEV cleavage after eluting my protein from the Ni-NTA column (TEV cleavage performed during overnight dialysis in imidazole free buffer as imidazole inhibits TEV activity at

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

I have always performed TEV cleavage after eluting my protein from the Ni-NTA column (TEV cleavage performed during overnight dialysis in imidazole free buffer as imidazole inhibits TEV activity at high concentrations). I use TCEP as reducing agent and have never included EDTA, by this method I've

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi Rafael, In this case I recommend the use of Zn-charged Chelating Sepharose™ Fast Flow (Cytiva), as we have established it in the early days when first applying IMAC to the purification of native antibody fragments (see PMID: 1367302 and PMID: 8163179). While the Zn-IDA matrix has slightly

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Dear Rafael In addition to the excellent suggestions already offered by previous responders, I can attest that Ni-penta resin (sold among others by a company with an odd name Marvelgent) is resistant to EDTA and DTT, and it leaks very little Ni (almost none). Plus, it elutes with low inidazole, ow

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi Rafael, Dom et al, Indeed nickel eluted with imidazole stays on all your proteins with a His tag, and addition of DTT will make almost all proteins go brown and crap out at this point. But, as Dom says, addition of EDTA before the DTT will solve the problem. Most of the time… If your protei

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

n, UK == about.me/david_briggs<http://about.me/david_briggs> From: CCP4 bulletin board on behalf of Rafael Marques Sent: Tuesday, October 31, 2023 7:21:21 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage External Sender:

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi Rafael, Once I inherited a protocol with a problem similar to yours and they told me that the precipitation was caused by nickel leaking out during the elution with 250 mM imidazole. I am not sure whether this was true, however, their fix was to place something like 20 ul of 200 mM EDTA at t

Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi Rafael, the simple answer is that the EDTA and DTT( or any other reducing agent) is not required for the TEV activity. You can have your TEV protease in 20 mM Tris pH 7.5 (RT) and 150 mM NaCl, plus either 10% Glycerol or 50% glycerol depending on storage conditions preference - 80 or -20C respec

[ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi everyone, I have been looking on this bb and other websites as well but I could not find a veredict. We are suspecting that when I elute my sample from my Ni-NTA column, the imidazole concentration (250 mM) is making it to precipitate. Once my sample has a cleavable TEV site, I was planning

[ccp4bb] [Off-topic] Research Coordinator position available immediately

There is a Research coordinator position available immediately at University of Oklahoma. *Research Coordinator* *-* *Job Number:* *230192* *Organization:* Chemistry/Biochemistry *Job Location:* Oklahoma-Norman-Norman Campus *Schedule:* Full-time *Work Schedule:* 8 AM - 5 PM Monday - Friday

[ccp4bb] [Off-topic] NMR manager position available

There is a NMR manager position available at University of Oklahoma. *NMR Manager position at University of Oklahoma * *Organization:* Chemistry/Biochemistry *Job Location:* Oklahoma-Norman-Norman Campus *Schedule:* Full-time *Work Schedule:* Monday - Friday 8-5 *Salary Range:* Target Sa

Re: [ccp4bb] Off topic - 3D models demonstrating drug binding to students

These magnetically-linking atomically accurate single-atom models look interesting - but I understand they might be too detailed or might shift around inadvertently :Snatoms Online Store - Magnetic Molecular Models for Educationsnatoms.comI also cannot say I used them (but I am always looking for a

Re: [ccp4bb] Off topic - 3D models demonstrating drug binding to students

] Off topic - 3D models demonstrating drug binding to students Dear CCP4, I apologize if this is off topic but I thought this may be a good community to ask. Before we re-invent the wheel this end, we are looking for any commercial source of three-dimensional macromolecular models that can be

[ccp4bb] Off topic - 3D models demonstrating drug binding to students

Dear CCP4, I apologize if this is off topic but I thought this may be a good community to ask. Before we re-invent the wheel this end, we are looking for any commercial source of three-dimensional macromolecular models that can be used to teach how drugs are designed to fit to protein targets.

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Oleksiy, If the Akta Start is just for loading affinity columns (without air sensor or software), why not to get a Cyvita peristaltic pump for £3k rather than £9k? They

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

.jpg@01D73AFE.96DB0800] From: CCP4 bulletin board On Behalf Of Kovtun, Oleksiy Sent: Friday, September 16, 2022 8:48 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure Hi Eike, Instead of AKTA Pure I purchased a combo of AKTA Go (€24k with air sensor

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Eike, Instead of AKTA Pure I purchased a combo of AKTA Go (€24k with air sensor) and AKta start (€9k with frac collector). It provides two parallel workstations with AKTA start handling the dirty job of loading lysates onto affinity cartridges and the GO doing clean SEC stages. For multiple

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Stephan, You’re mostly correct, however, gradients made on instrument with one pump are less reliable. Thank you. Vaheh From: CCP4 bulletin board On Behalf Of Stephan Rempel Sent: Friday, September 16, 2022 7:38 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic: experience

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Eike, we bought a Biorad back in 2005 or so, an Akta Purifier in 2011 and recently an Akta Go (2020). All work(ed) well, the latter two are in use. Don't know about the Biorad, it's in Santiago de Compostela. Both the Biorad and Purifier have been used a lot, the Akta Go not that much so far.

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

mber 2022 13:17 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure Hello Eike, A couple of comments on the AKTA Pures (while I have not experience with the BioRad systems). We have a couple of AKTA Pures and we (and another lab close to us) had a

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

ry soon. Hth, Marko Marko Hyvonen Department of Biochemistry University of Cambridge https://hyvonen.bioc.cam.ac.uk @HyvonenGroup mh...@cam.ac.uk<mailto:mh...@cam.ac.uk> From: CCP4 bulletin board On Behalf Of Schulz, Eike-Christian Sent: 16 September 2022 08:40 To: CCP4BB@JISCMAIL.A

[ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Dear all, I am considering to purchase a chromatography system for routine protein purification. The device is supposed to be used in a multi-user environment, hence ease of use, ease of training and ease of maintenance is important. I am rather looking for a robust system people like to use th

Re: [ccp4bb] Off-topic question related to ITC binding studies

Hi Gergő, Thank you for discussing it in detail. It is a great help. Based on the curve fit, we also believe that the two proteins bind independently to the DNA duplex with the same affinity. The DNA indeed contains 2 sites for protein binding. As far as the dimerization of protein is concerned, w

[ccp4bb] RES: [ccp4bb] Off-topic question related to ITC binding studies

16 de agosto de 2022 14:41 Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Assunto: [ccp4bb] Off-topic question related to ITC binding studies Hello everyone! Hope this email finds you well. I have an off-topic question regarding ITC binding studies, which was asked by a reviewer

[ccp4bb] Off-topic question related to ITC binding studies

Hello everyone! Hope this email finds you well. I have an off-topic question regarding ITC binding studies, which was asked by a reviewer. We performed an ITC binding study (using Affinity ITC, TA Instruments) to evaluate protein-DNA interaction which resulted in a perfect sigmoidal curve. We

Re: [ccp4bb] Off topic: Are all storage dewars equal?

Hi Carmien, It appears to me that the dewars in your first link are those made by Taylor Wharton. You can do a search with that name and hopefully find less expensive options. We've used them for many years and they are good. Another one, you could try, is VME https://mvebio.com/aluminum-dewars/ We

[ccp4bb] Off topic: Are all storage dewars equal?

Hello everyone, We want to buy a storage dewar that can house seven unipucks. I have seen dewars on the website of the crystallography suppliers (such as the HC35 https://www.moleculardimensions.com/products/high-capacity-cryogenic-refrigerators), which is significantly more expensive than the run

Re: [ccp4bb] Off-topic: Mol* Viewer re-centring hack

Hi Jon, Glad to see you are making use of the Mol* viewer. I am also replying on-list as others will hopefully find my response useful. For your specific case, I would recommend using the 'selection mode' - activated by clicking the cursor icon at the top right of the viewport. This mode cha

[ccp4bb] Off-topic: Mol* Viewer re-centring hack

I have been using Mol* recently which looks like a really nice tool for basic model viewing. When you click off an atom (which is the normal action for clearing a selection in model viewers), it changes the zoom, orientation and re-slabs it. Has anyone found a hack to stop it re-centring/zoomin

[ccp4bb] Off-topic - Cryojet5 - boiloff heater spare

Dear, My apologies for the off-topic post. Our (11-years old) Oxford Instruments Cryojet5 LN2 cryocooling has broken down and to repair this, we are looking to purchase a simple ‘boiloff heater’ element for the shield flow dewar leg. Oxford Instruments informed us that this product was discon

[ccp4bb] Off topic: docking for biological insight

Dear ccp4 community, When I see the (improving) accuracy of docking in generating drug like leads, I see predominantly synthetic compounds used. It occurred to me that it might be informative to use docking of naturally occurring compounds (amino acids, sugars, lipids, factors etc) to find pocke

Re: [ccp4bb] off topic -- pymol error message

; To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] off topic -- pymol error message > > Hi all, > > What does this message means when loading a pdb file in pymol: > > 'utf-8' codec can't decode byte 0xd6 in position 37: invalid continuation byte > > Thank you! > >

[ccp4bb] off topic -- pymol error message

Hi all, What does this message means when loading a pdb file in pymol: 'utf-8' codec can't decode byte 0xd6 in position 37: invalid continuation byte Thank you! Chen -- Cheng Chen, Associate ProfessorSchool of Life Sciences, Building 15, Tianjin University No.92 Weijin Road, Nankai Di

Re: [ccp4bb] Off topic - gene toxic for expression strains

Hi there, Somehow I've missed the original email :) Sorry! There are options for expressing really toxic genes, some of which have already been mentioned and others perhaps not: 1. tight regulation of expression (promoter, repressor, other regulatory elements, or a combination thereof). Beyond u

Re: [ccp4bb] Off topic - gene toxic for expression strains

Hi Andy, just to follow up on Christian suggestion, which is exactly the way to go. In case you are using an already pET based vector, simply try BL21-AI (https://www.thermofisher.com/order/catalog/product/C607003), which has the T7 RNA polymerase under arabinose promoter should do the trick. Al

Re: [ccp4bb] Off topic - gene toxic for expression strains

Hi Andy, have you tried another promotor? Arabinose is much tighter, just to be sure that it is really not leaking. Cheers Christian On Mon, Apr 4, 2022 at 10:20 AM Andrew Lovering wrote: > Dear Board, > > > > Perhaps off-topic, but in the wider scope it’s relevant to many on here. > > > > We

[ccp4bb] Off topic - gene toxic for expression strains

Dear Board, Perhaps off-topic, but in the wider scope it's relevant to many on here. We have a gene that we are able to clone, and propagate in DH5a etc non-expression cells (hence nucleotide sequence is non-toxic) But, when we attempt to transfer to an expression strain we get no colonies We

[ccp4bb] Off-topic: Replacement CCD camera for Rigaku Dekstop Minstrel UV imager

Hi, I know the chance is low, but in a desperate try to fix our long-serving Rigaku crystal imager I'm wondering whether anyone is aware of any decommissioned (Desktop) Minstrel systems around which could provide spare sparts. Unfortunately Rigaku crystal automation systems are no longer suppor

Re: [ccp4bb] Off-topic: happy 2022

For those who want to riddle it out (and as structural biologists hopefully don’t come across mirror and glide planes) a ppt where you can take the International Tables and superimpose the tetragonal PG diagrams on the Kaleidoscope. Find the unit cell first, and then ferret out the rest. htt

Re: [ccp4bb] Off-topic: happy 2022

Looks like no. 12 p4g to me: http://www2.clarku.edu/faculty/djoyce/wallpaper/wall12.html Happy New Year. On Sat, 1 Jan 2022 at 20:52, Bernhard Rupp wrote: > ..and which one of the 17 plane groups do we see here? > > Cherrs br > > On Sat, Jan 1, 2022, 12:47 Phoebe A. Rice wrote: > >> Apologies

Re: [ccp4bb] Off Topic: CCP4MG Help

5, 2021 2:21 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Off Topic: CCP4MG Help > > CAUTION: This email comes from an external source; the attachments and/or > links may compromise our secure environment. Do not open or click on > suspicious emails. Please click on t

Re: [ccp4bb] Off Topic: CCP4MG Help

@JISCMAIL.AC.UK Subject: [ccp4bb] Off Topic: CCP4MG Help CAUTION: This email comes from an external source; the attachments and/or links may compromise our secure environment. Do not open or click on suspicious emails. Please click on the “Phish Alert” button on the top right of the Outlook

[ccp4bb] Off Topic: CCP4MG Help

Hello all, Looking for advice from any CCP4MG users. I am making some structural figures and have run into a problem. I posted a question to the CCP4MG-specific email list quite a while ago but received no responses, so I don't know if that list is still active. Essentially, I want to change

[ccp4bb] (off topic) Job posting - Associate Director, Protein Science

https://boards.greenhouse.io/roivantsciences/jobs/3549863 Finally posted! Thank you for looking. Artem - Cosmic Cats approve of this message To unsubscribe from the CCP4BB list, click the following link: https://www.jiscm

Re: [ccp4bb] off-topic: pH meters

The meter doesn't matter so much as the electrode. For the meter, anything with at least 3 pH multipoint calibration is sufficient for most purposes. I usually bought something from our preferred university vendor a with very large LCD displays for my aging eyes. For protein work, and especially i

[ccp4bb] off-topic: pH meters

Fellow protein biochemists: My ~30 year-old Beckman pH meter is finally showing its age, and I’m looking for a high-quality replacement that doesn’t cost insane amounts of money. My initial thoughts gravitate toward Mettler (it’s a name I trust, and some of their low-end instruments aren’t absu

Re: [ccp4bb] Off topic, protein characterization and binding job opportunity in Boston area

Here is the right link - https://www.linkedin.com/jobs/view/2662640332/?refId=cCDvahCrRPGWslMQWMgEOQ%3D%3D Thank you everyone for pointing this out. On Mon, Aug 23, 2021 at 4:18 PM Yanfeng Zhou wrote: > Hi, > > Sorry for the off topic post. If anyone is passionate about working in an > excit

[ccp4bb] Off topic, protein characterization and binding job opportunity in Boston area

Hi, Sorry for the off topic post. If anyone is passionate about working in an exciting biotech powered by machine learning, structural biology, and protein engineering, around Boston in the US, please check the link below. https://www.linkedin.com/jobs/view/2662640332/efId=cCDvahCrRPGWslMQWMgEOQ%

Re: [ccp4bb] off-topic: structural motif / domain comparison

sday, August 5, 2021 23:43 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] off-topic: structural motif / domain comparison Dear all Sorry for an off-topic question here. I wonder if anyone may be aware of any search program which allows one to 'blast' a protein domain just like we &#x

Re: [ccp4bb] off-topic: structural motif / domain comparison

You might also find use in databases such as pfam, ecod, cath, scop. Orly On Fri, Aug 6, 2021, 09:43 Sam Tang wrote: > Dear all > > Sorry for an off-topic question here. I wonder if anyone may be aware of > any search program which allows one to 'blast' a protein domain just like > we 'blast' a

Re: [ccp4bb] off-topic: structural motif / domain comparison

Perhaps DALI can be of use http://ekhidna2.biocenter.helsinki.fi/dali/ Dal. pá 6. 8. 2021 v 8:42 odesílatel Sam Tang napsal: > Dear all > > Sorry for an off-topic question here. I wonder if anyone may be aware of > any search program which allows one to 'blast' a protein domain just like > we '

Re: [ccp4bb] off-topic: structural motif / domain comparison

Hi Sam, Perhaps the Modeller service is what you’re looking for? https://salilab.org/modeller/ Best wishes, Chris On Fri, 6 Aug 2021 at 07:42 Sam Tang wrote: > Dear all > > Sorry for an off-topic question here. I wonder if anyone may be aware of > any search program which allows one to 'blas

Re: [ccp4bb] off-topic: structural motif / domain comparison

Like PDBeFOLD search? https://www.ebi.ac.uk/msd-srv/ssm/ Jan On Fri, Aug 6, 2021 at 8:43 AM Sam Tang wrote: > Dear all > > Sorry for an off-topic question here. I wonder if anyone may be aware of > any search program which allows one to 'blast' a protein domain just like > we 'blast' a protein

[ccp4bb] off-topic: structural motif / domain comparison

Dear all Sorry for an off-topic question here. I wonder if anyone may be aware of any search program which allows one to 'blast' a protein domain just like we 'blast' a protein sequence? For example I have an epitope in hand and would like to find out whether this also exists in other proteins. Mo

Re: [ccp4bb] off-topic: glycans

> *From:* CCP4 bulletin board on behalf of Sam Tang > > *Sent:* 29 June 2021 06:12 > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] off-topic: glycans > > > *External Sender:* Use caution. > > Dear community > > Sorry for an off-topic question here

Re: [ccp4bb] off-topic: glycans

== about.me/david_briggs From: CCP4 bulletin board on behalf of Sam Tang Sent: 29 June 2021 06:12 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] off-topic: glycans External Sender: Use caution. Dear community Sorry for an off-topic question here. I wonder if

[ccp4bb] off-topic: glycans

Dear community Sorry for an off-topic question here. I wonder if anyone may be aware of any glycan modification database where we can predict what is what. For example, if I got a mass difference of m/z X on LC-MS, and I would like to have a rough idea what it might be, where should I go for? Tha

[ccp4bb] Off-topic: research associate job posting

Dear CCP4 friends We are looking for fresh talent :) please see the posting below or directly at MassBio. https://careers.massbio.org/jobs/?keywords=Research+Associate+Silicon+Therapeutics&location=Boston%2C+MA%2C+United+States&location_completion=city%3DBoston%24state%3DMassachusetts%24country%3

Re: [ccp4bb] OFF TOPIC question

nt: Thursday, November 26, 2020 8:59 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] OFF TOPIC question Hi all, I have two constructs having different ori, p15ori and M13 ori, different promoters araC and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I would

Re: [ccp4bb] OFF TOPIC question

, November 26, 2020 2:48:24 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] AW: [ccp4bb] OFF TOPIC question Bl21Pro j Von: CCP4 bulletin board Im Auftrag von Anamika Singh Gesendet: Donnerstag, 26. November 2020 11:07 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] OFF TOPIC question One

[ccp4bb] AW: [ccp4bb] OFF TOPIC question

Bl21Pro j Von: CCP4 bulletin board Im Auftrag von Anamika Singh Gesendet: Donnerstag, 26. November 2020 11:07 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] OFF TOPIC question One correction to the previous question: I have two constructs having different ori, p15ori and M13 ori, different

Re: [ccp4bb] OFF TOPIC question

*One correction to the previous question:* I have two constructs having different ori, p15ori and M13 ori, different promoters *araBAD promoter* and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I would like to know which E. coli host cells will be good for

[ccp4bb] OFF TOPIC question

Hi all, I have two constructs having different ori, p15ori and M13 ori, different promoters araC and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I would like to know which *expressing E. coli host cells* will be good for the co-transformation of these con

Re: [ccp4bb] (off-topic) beamline for XAFS

Dear Banu, I would recommend looking also at SSRL beam line 7-3 and 9-3 as they are well set up for protein EXAFS. Greetings, Jan On Tue, Nov 10, 2020 at 11:03 AM PULSARSTRIAN wrote: > Dear all, > Sorry for the off topic. > Looking for suggestions on beamlines for XAFS on proteins

[ccp4bb] (off-topic) beamline for XAFS

Dear all, Sorry for the off topic. Looking for suggestions on beamlines for XAFS on proteins with [4Fe-4S] clusters: I have two options, one at BNL and another at APS@ANL. At BNL, I think 6-BM (BMM) seems to be more for biological samples on metalloproteins on the other hand, ANL has

Re: [ccp4bb] Off-topic: Mild cross-linking protocol

If you have hopes for cysteine residues in reasonable proximity, then bis-iodoacetamide (with a suitable spacer, commercially available is the ethylenediamine spacer). Any reasonable chemist can make you other spacer lengths to order. Homobifunctional PEG with maleimide, N-hydroxy succinimide este

[ccp4bb] Off-topic: Mild cross-linking protocol

Dear all, Sorry for the off-topic question. I need to prepare an heterodimeric protein complex for an immunisation, and I would like to make sure that the dimer will be stable and it won't dissociate after injection. I am wondering if any of you has a mild cross-linking protocol (and suggestio

[ccp4bb] Off-topic: amphipathic polymer NV-10

Dear all,Sorry for an extremely off-topic question. We are looking for an amphipathic polymer, NV-10. It used to be made by Expedeon and has been used for assisting proper protein folding as latest as in 2019 as gathered from the literature. Now in the year 2020, it seems to have disappeared fro

[ccp4bb] [OFF TOPIC] Cell biology bb

Hi Folks, how are you doing? Hope everyone is safe! I was wondering if there is a bulletin board email group like this one concerning Cell Biology, where we could find not only discussions about related topics but also post-doc opportunities. I would be very glad if you guys know any and could

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