Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Sridhar Srinivasan
Hi All, I installed the toolshed htseq_bams_to_count_matrix repository , i gave the gene model gtf file(ex: tss.gtf file) Gene model (GFF) file to count reads over from your current history: in this section and all bam files. it gives error as empty format: tabular, database: hg19 Traceback

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Ross
Sridhar, The error message you provide suggests that the HTSeq python module is not available when Galaxy tries to run the tool. Please confirm that you installed that tool through the admin interface from the test toolshed and that the admin interface for toolshed installed tools shows the

Re: [galaxy-dev] Biopython dependency on matplotlib

2013-08-21 Thread Bjoern Gruening
Hi Greg, that bug was fixed I think! Thanks, Bjoern Hello Peter and Bjoern, Can you clarify the tool shed bug to which you are referring with the statement: Still that TS bug needs to be fixed I think before the next stable release. I'll take a look as soon as I get

Re: [galaxy-dev] Action type 'template_command' on galaxy-dist?

2013-08-21 Thread Bjoern Gruening
Hi, also fixed, yes! Good start in a day, to write positive mails :) Bjoern Hello Bjoern, I believe this issue was corrected in changeset 10412:29ab5a6d75a7, which was committed to the stable branch and is running on both tool sheds. At least I am unable to reproduce the behavior you

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Sridhar Srinivasan
Hi Ross, i Installed through admin interface . for pysam and freetype it showed new... and rest installed.. Thanks On Wed, Aug 21, 2013 at 11:47 AM, Ross ross.laza...@gmail.com wrote: Sridhar, The error message you provide suggests that the HTSeq python module is not available when

Re: [galaxy-dev] Tool Shed upload/migration revision checking bug?

2013-08-21 Thread Bjoern Gruening
Morning Greg, seems to be also fixed in latest release. Will reopen the thread if I encounter it again. Thanks a lot, Bjoern Hello Peter and Bjoern, I believe this issue was handled in the August Galaxy release, but the main tool shed had not yet been upgraded to that release when you

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Ross
Sridhar, Kindly try rerunning the failed job to confirm that it still fails now that the tool installation should have well and truly finished - it does take a while. If it still fails the same way, please send me a copy of the installation log for the htseq dependency found in: [your

Re: [galaxy-dev] One installable revision is not working for orphan-tool-dependencies - Was: Action type 'template_command' on galaxy-dist?

2013-08-21 Thread Bjoern Gruening
Hi Greg, a first test seems to work as expected for me, will test again with the whole CTB. If I understood your commit you are searching now for the next valid 'installable' revision, aka a revision with metadata. Does it make sense to have revisions for Tool dependency definition. Only one

Re: [galaxy-dev] Passing user_email to a data source

2013-08-21 Thread Hans-Rudolf Hotz
Hi Anthony I doubt it will solve your problem, but just as a general hint: you can use the email address for filtering. In our case, the user gets a different list of groups he/she can select from, eg: param name=group type=select label=Select your group options

Re: [galaxy-dev] Galaxy Release Cycle Length

2013-08-21 Thread Hans-Rudolf Hotz
Hi Dave two months is a good time between releases. Much more important than the release cycle length is fixing identified bugs on the release branch as well. Regards, Hans-Rudolf On 08/20/2013 08:36 PM, Dave Clements wrote: Hello all, At one of the GCC2013 Birds of a Feather sessions

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Sridhar Srinivasan
Hi Ross, I tried installing again it shows no item.. Also i couldn't find any .log file or the ( /htseq/0.5.4p3/fubar/htseq_bams_to_count_matrix) in the tool dependency directory. Thanks On Wed, Aug 21, 2013 at 12:13 PM, Ross ross.laza...@gmail.com wrote: Sridhar, Kindly try rerunning the

[galaxy-dev] Troubleshooting file uploads (to Data Library)

2013-08-21 Thread Clare Sloggett
Hi, I am having trouble uploading files to a Data Library, and I'm not sure where to begin troubleshooting. I'm uploading from a URL (but I had a similar issue from the desktop). The symptom is that the datasets in the library have the message This job is queued and never seem to progress. I am

Re: [galaxy-dev] Troubleshooting file uploads (to Data Library)

2013-08-21 Thread Clare Sloggett
Sorry, missed some information: there are a handful of files, 35MB (gzipped) each. The issue occurs even if I only try to upload one of them though. The server is a 16-core machine. On 21 August 2013 17:08, Clare Sloggett s...@unimelb.edu.au wrote: Hi, I am having trouble uploading files to

Re: [galaxy-dev] DESeq for Galaxy

2013-08-21 Thread Ross
Sridhar, Tool shed installations always create those log files, even when they fail. I am unable to offer any further assistance without seeing the installation log for the dependency. What does: find [your tool dependency directory] -name INSTALLATION.log show? If necessary, try navigating

Re: [galaxy-dev] Troubleshooting file uploads (to Data Library)

2013-08-21 Thread Hans-Rudolf Hotz
Hi Clare a few points to start: - do you define the 'File Format'? (don't use 'Auto-detect' for big files) - and similar to a recent question on the list: check your proxy settings Regards, Hans-Rudof On 08/21/2013 09:09 AM, Clare Sloggett wrote: Sorry, missed some information:

Re: [galaxy-dev] Galaxy Release Cycle Length

2013-08-21 Thread Ido Tamir
Why the dislike for quick turnover? Could somebody present the arguments for people not having been at the BOF? People don't have to upgrade - unless its breaking changes that e.g. disable the possibility to download from the public toolshed which forced me to upgrade. The alternative to

Re: [galaxy-dev] Galaxy Release Cycle Length

2013-08-21 Thread Peter Cock
On Wednesday, August 21, 2013, Ido Tamir wrote: Why the dislike for quick turnover? Could somebody present the arguments for people not having been at the BOF? People don't have to upgrade - unless its breaking changes that e.g. disable the possibility to download from the public toolshed

Re: [galaxy-dev] Duplicated entries in Manage installed tool shed repositories table

2013-08-21 Thread Peter Cock
On Tue, Aug 20, 2013 at 8:34 PM, Greg Von Kuster g...@bx.psu.edu wrote: Peter and Ross, I've not been able to reproduce this behavior, most likely because I am not following the precise steps you've used when you see it. Can you clarify what you do when you encounter it? Thanks! Greg Von

Re: [galaxy-dev] Tool Shed packages for BLAST+ binaries

2013-08-21 Thread Peter Cock
On Tue, Aug 20, 2013 at 9:15 PM, Dave Bouvier d...@bx.psu.edu wrote: Peter, I believe this was again due to the installation of an earlier repository hanging until the testing framework canceled the build. I've added the repository in question to the exclude list, and hopefully your blast

Re: [galaxy-dev] Troubleshooting file uploads (to Data Library)

2013-08-21 Thread Clare Sloggett
Hi Hans and all, The issue turned out to be a more general one with our job runners (it was also stopping non-data-transfer jobs running, if I'd noticed). Yep, I do specify file format when uploading. Thanks for your help! Clare On 21 August 2013 17:38, Hans-Rudolf Hotz h...@fmi.ch wrote:

[galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Peter Cock
Hi Dannon, I'm seeing a problem which seems very similar to the one Bjoern had back in June with the same workflow (which appeared to have been fixed according to the old IUC thread Workflow import is not working). This server is running the current galaxy-dist release, $ sudo -u galaxy hg head

[galaxy-dev] Workflow action summary confusing, repeats Hide this dataset

2013-08-21 Thread Peter Cock
Hi all, I have found two potential bugs with how workflow actions are described to the user when running a workflow. I am looking over the run workflow dialogue for this workflow, (I currently get a warning due to the filter tool having a new Number of header lines to skip option)

Re: [galaxy-dev] Tool Shed packages for BLAST+ binaries

2013-08-21 Thread Peter Cock
On Wed, Aug 21, 2013 at 10:54 AM, Peter Cock p.j.a.c...@googlemail.com wrote: On Tue, Aug 20, 2013 at 9:15 PM, Dave Bouvier d...@bx.psu.edu wrote: Peter, I believe this was again due to the installation of an earlier repository hanging until the testing framework canceled the build. I've

Re: [galaxy-dev] Workflow action summary confusing, repeats Hide this dataset

2013-08-21 Thread Dannon Baker
Peter, Thanks for noticing this -- the text in the UI should definitely reflect which output it's referring to. Hide output_pos, etc. https://trello.com/c/KAtwLSiV/1069-workflow-actions-cosmetic-issues -Dannon On Wed, Aug 21, 2013 at 7:16 AM, Peter Cock p.j.a.c...@googlemail.comwrote: Hi

Re: [galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Dannon Baker
Hey Peter, I've reproduced this here, thanks. Unfortunately this got missed previously, once we had the import itself working. To be clear, though, the workflow itself is still completely functional, imports and runs, and the only issue is the inability to view the svg? I think what's causing

Re: [galaxy-dev] Tool Shed packages for BLAST+ binaries

2013-08-21 Thread Dave Bouvier
Peter, Here is the relevant log output for the installation of ncbi_blast_plus. A quick google informs me that exit code 64 means command line usage error, for what it's worth. install_and_test_repositories INFO 2013-08-20 23:34:43,270 Installing and testing revision 946749c8605f of

Re: [galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Dannon Baker
Quick followup on this one -- had you manually tweaked this workflow prior to upload? I see all of the tool identifiers are the short form 'signalp3' and not the long-form toolshed included versions. On Wed, Aug 21, 2013 at 8:25 AM, Dannon Baker dannon.ba...@gmail.comwrote: Hey Peter, I've

Re: [galaxy-dev] Tool Shed packages for BLAST+ binaries

2013-08-21 Thread Peter Cock
On Wed, Aug 21, 2013 at 1:45 PM, Dave Bouvier d...@bx.psu.edu wrote: Peter, Here is the relevant log output for the installation of ncbi_blast_plus. A quick google informs me that exit code 64 means command line usage error, for what it's worth. install_and_test_repositories INFO 2013-08-20

Re: [galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Peter Cock
On Wed, Aug 21, 2013 at 8:25 AM, Dannon Baker dannon.ba...@gmail.com wrote: Hey Peter, I've reproduced this here, thanks. Unfortunately this got missed previously, once we had the import itself working. To be clear, though, the workflow itself is still completely functional, imports and

Re: [galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Dannon Baker
On Wed, Aug 21, 2013 at 9:10 AM, Peter Cock p.j.a.c...@googlemail.comwrote: Via the Tool Shed I couldn't see the import workflow action... from memory it was on the broken SVG page? Yep, that's where it was. These are not tweaked - it is an old workflow created with manually installed tools

Re: [galaxy-dev] Duplicated entries in Manage installed tool shed repositories table

2013-08-21 Thread Greg Von Kuster
Thanks Peter, As soon as I can get the steps to produce white ghost, I'll get a fix committed - I've not been able to produce one yet. Greg Von Kuster On Aug 21, 2013, at 5:41 AM, Peter Cock p.j.a.c...@googlemail.com wrote: On Tue, Aug 20, 2013 at 8:34 PM, Greg Von Kuster g...@bx.psu.edu

Re: [galaxy-dev] Error granting permissions on new Tool Shed repository

2013-08-21 Thread Peter Cock
I've made a note on the Trello issue Ross filed for this, https://trello.com/c/SqrjrnNg/986 On Wed, Aug 7, 2013 at 4:16 PM, Greg Von Kuster g...@bx.psu.edu wrote: Hi Peter, This is a known issue ( not a Tool Shed issue, but a Galaxy issue ) related to the Select list that gets automatically

Re: [galaxy-dev] One installable revision is not working for orphan-tool-dependencies - Was: Action type 'template_command' on galaxy-dist?

2013-08-21 Thread Greg Von Kuster
Hello Bjoern, On Aug 21, 2013, at 2:51 AM, Bjoern Gruening bjoern.gruen...@gmail.com wrote: Hi Greg, a first test seems to work as expected for me, will test again with the whole CTB. Great - thanks! If I understood your commit you are searching now for the next valid 'installable'

Re: [galaxy-dev] Error granting permissions on new Tool Shed repository

2013-08-21 Thread Greg Von Kuster
Thanks Peter, I've added some comments as well, and relabeld the Trello card as trivial bug: Using Galaxy select lists fail unless the Select one option removed first. since this is not a tool shed issue, but a Selectlist issue in the Galaxy formbuilder framework. The tool shed simply

Re: [galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Dannon Baker
Hey Peter, The reason it's actually failing has to do with the tool Filter1, and your particular version of the filter tool not having the header lines parameter. This was added in 8678:a14006775b08. In this instance, we should have just used the default value. Once all tools and versions are

Re: [galaxy-dev] Workflow import is not working, KeyError: 'header_lines'

2013-08-21 Thread Peter Cock
n Wed, Aug 21, 2013 at 2:59 PM, Dannon Baker dannon.ba...@gmail.com wrote: Hey Peter, The reason it's actually failing has to do with the tool Filter1, and your particular version of the filter tool not having the header lines parameter. This was added in 8678:a14006775b08. In this instance,

[galaxy-dev] Editing workflow - automatic update changes not saved?

2013-08-21 Thread Peter Cock
Hi all, I'm trying to update one of my saved workflows which was created using an old version of the filter tool BEFORE this tool had the option Number of header lines to skip. In order to get rid of the nag message on using the tool, I want to re-save the workflow using the default 0 for this.

Re: [galaxy-dev] Passing user_email to a data source

2013-08-21 Thread Anthony Bretaudeau
Hi, I didn't know this was possible, thanks! Unfortunately I can't use this in my case because I can only use param with type=hidden for a data source... Anthony On 21/08/2013 08:51, Hans-Rudolf Hotz wrote: Hi Anthony I doubt it will solve your problem, but just as a general hint: you can

[galaxy-dev] Non-ascii characters in password break Galaxy login

2013-08-21 Thread Peter Cock
Hi all, I've seen several unicode issues reported recently, so here is another one: I just tried to log in and naively copy and pasted my password from one browser to another: This upset Galaxy, 143.234.100.162 - - [21/Aug/2013:15:47:55 +0100] POST /galaxy/user/login?use_panels=False

Re: [galaxy-dev] JSON error trying to update Tool Shed workflow

2013-08-21 Thread Greg Von Kuster
Hi Peter, You are probably using a mac if you are seeing this, so can you check to see if your .ga file permissions include an @ sign? This an Apple osx issue, and you'll need to do the following: xattr -d com.apple.quarantine name of your exported.ga file When you do this, it will fix the

Re: [galaxy-dev] JSON error trying to update Tool Shed workflow

2013-08-21 Thread Peter Cock
On Wed, Aug 21, 2013 at 4:30 PM, Greg Von Kuster g...@bx.psu.edu wrote: Hi Peter, You are probably using a mac if you are seeing this, so can you check to see if your .ga file permissions include an @ sign? Yes, I am, and yes, they have an '@' sign. This an Apple osx issue, and you'll need

Re: [galaxy-dev] Embedding a help text in a Workflow?

2013-08-21 Thread Peter Cock
On Mon, Aug 19, 2013 at 3:34 PM, Peter Cock p.j.a.c...@googlemail.com wrote: Filed on Trello, https://trello.com/c/HaQnZGvW/1060-embedding-help-text-in-workflows-ideally-rich-text-with-markup Peter On Fri, May 10, 2013 at 5:07 PM, Jeremy Goecks jeremy.goe...@emory.edu wrote: Do you think we

Re: [galaxy-dev] Embedding a help text in a Workflow?

2013-08-21 Thread Peter Cock
On Wed, Aug 21, 2013 at 5:40 PM, Peter Cock p.j.a.c...@googlemail.com wrote: On Mon, Aug 19, 2013 at 3:34 PM, Peter Cock p.j.a.c...@googlemail.com wrote: Filed on Trello, https://trello.com/c/HaQnZGvW/1060-embedding-help-text-in-workflows-ideally-rich-text-with-markup Peter On Fri, May 10,

Re: [galaxy-dev] Bismark issue

2013-08-21 Thread Bjoern Gruening
Hi Shane, please find a new bismark version with an updated dependency on samtools 0.1.19 here: http://toolshed.g2.bx.psu.edu/view/bgruening/bismark Thanks for reporting that issue, Bjoern I've had a bit of a time trying to get Bismark working on our Galaxy instance. The install doesn't

Re: [galaxy-dev] One installable revision is not working for orphan-tool-dependencies - Was: Action type 'template_command' on galaxy-dist?

2013-08-21 Thread Bjoern Gruening
Hi Greg, seems to work fine! Hello Bjoern, On Aug 21, 2013, at 2:51 AM, Bjoern Gruening bjoern.gruen...@gmail.com wrote: Hi Greg, a first test seems to work as expected for me, will test again with the whole CTB. Great - thanks! If I understood your commit you are

Re: [galaxy-dev] [galaxy-iuc] Duplicated entries in Manage installed tool shed repositories table

2013-08-21 Thread Bjoern Gruening
We should create a Tool Shed Hackathon, I'm an expert in producing 'white ghosts' :) Thanks Peter, As soon as I can get the steps to produce white ghost, I'll get a fix committed - I've not been able to produce one yet. Greg Von Kuster On Aug 21, 2013, at 5:41 AM, Peter Cock

Re: [galaxy-dev] One installable revision is not working for orphan-tool-dependencies - Was: Action type 'template_command' on galaxy-dist?

2013-08-21 Thread Greg Von Kuster
Hello Bjoern, On Aug 21, 2013, at 1:27 PM, Bjoern Gruening bjoern.gruen...@gmail.com wrote: Hi Greg, seems to work fine! Hello Bjoern, On Aug 21, 2013, at 2:51 AM, Bjoern Gruening bjoern.gruen...@gmail.com wrote: Hi Greg, a first test seems to work as expected for me, will

[galaxy-dev] Why does my trackster complain about not being able to display BED files?

2013-08-21 Thread Anthonius deBoer
Hi,I am using the latest version of galaxy at my local installation and I try to use trackster to display a simple BED file, but it invariably complains about not having a converter for this datatype and it tells me to check mydatatypes_conf.xml which I did (not sure what I should be looking for,

Re: [galaxy-dev] Why does my trackster complain about not being able to display BED files?

2013-08-21 Thread sam guerler
Hi Anthonius, Do you have the BEDTools installed and its directory added to your PATH environment variable? Trackster uses compressed file formats to reduce the amount of data transfers. I hope this helps, Sam On Wed, Aug 21, 2013 at 4:11 PM, Anthonius deBoer thondeb...@me.com wrote: Hi,

Re: [galaxy-dev] Bismark issue

2013-08-21 Thread Shane Sturrock
Hi Bjoern, I've upgraded our machine and confirmed it now works fine. Thanks for that! Shane On 22/08/2013, at 5:17 AM, Bjoern Gruening bjoern.gruen...@gmail.com wrote: Hi Shane, please find a new bismark version with an updated dependency on samtools 0.1.19 here:

[galaxy-dev] error installing dependency for tool shed repository.

2013-08-21 Thread Curt Palm
I've just created a repository in the sandbox and have been trying to test the installation of a dependency my tool needs. The tool needs the python module requests and I've written code. I get an error: STDERR python: can't open file 'setup.py': [Errno 2] No such file or directory I've

Re: [galaxy-dev] Why does my trackster complain about not being able to display BED files?

2013-08-21 Thread Anthonius deBoer
Yep...$ bedtools --versionbedtools v2.17.0ThonOn Aug 21, 2013, at 01:40 PM, sam guerler aysam.guer...@gmail.com wrote:Hi Anthonius,Do you have the BEDTools installed and its directory added to your PATH environment variable? Trackster uses compressed file formats to reduce the amount of data

Re: [galaxy-dev] error installing dependency for tool shed repository.

2013-08-21 Thread Dave Bouvier
Curt, I tried installing the ingtest repository on a local instance of Galaxy, and the requests python module installed without errors. Which revision of Galaxy are you trying to install this repository into, and what kind of environment are you running on that system? --Dave B. On

Re: [galaxy-dev] error installing dependency for tool shed repository.

2013-08-21 Thread Curt Palm
Hi Dave, I made changes giving the suggestions of Ross, I got a different error and that gave me insight as to what was going on. I uploaded new xml: ?xml version=1.0? tool_dependency package name=requests version=1.2.3 install version=1.0 actions action

Re: [galaxy-dev] error installing dependency for tool shed repository.

2013-08-21 Thread Dave Bouvier
Curt, I'm happy to hear that everything is now working. If you encounter any further issues, please don't hesitate to contact us. --Dave B. On 2013-08-21 21:31, Curt Palm wrote: Hi Dave, I made changes giving the suggestions of Ross, I got a different error and that gave me insight as to