Re: [Histonet] Ihc control tissues

Charles, I am in a research only lab as well. We create TMA's as control blocks using the same idea that Val uses. Multiple tissues per block for all IHC and also good for special stains. You can make nicely organized TMA's manually very cost effectively as well. Feel free to reach out to me if

Re: [Histonet] Ihc control tissues

Awesome thank you Val. I like the multi control option. Can save money in the end by using less reagents On Wed, May 8, 2024 at 10:52 AM Val T wrote: > Hey Charles- > > Our research lab prepares a “multimouse” control. We embed 10 organs on > the same slide and it serves as a +/- control for

Re: [Histonet] Ihc control tissues

Hey Charles- Our research lab prepares a “multimouse” control. We embed 10 organs on the same slide and it serves as a +/- control for just about every IHC marker. However, if you don’t want to do that- here are some tissues that would make nice controls. > BCL-2- spleen, lymph nodes >

[Histonet] Ihc control tissues

Hello all, I am trying to put together a positive and negative tissue control list for the following antibodies at my research core. I came over from a clinical pathology background and we used tonsils for a lot of these antibodies. Since rats and mice don't have tonsils I am looking for

Re: [Histonet] IHC Validation Question

Hi Kara, Use multi-tissue block controls with normal tissues that are treated exactly the same (pre-analytically speaking) as your routine surgical specimens. Consult NordiQC.org for recommended normal control tissue for each IHC marker. The most used multi-tissue control block in my lab has

[Histonet] IHC Validation Question

Morning Histo Friends. Got a quick question for you. I am getting ready to go over to a new start up lab that will be doing all IHC staining in house. I have set up a lab before and done validations but that was on equipment and special stains which I will be doing again and feel pretty good

[Histonet] IHC

Greetings Histonet community, I'm interested in learning if anyone has experience withperforming Von Willebrand factor and alpha smooth muscle actin IHC staining onpig tissues (FFPE). If you do, could you please share the protocol along withdetails about the antibodies used, including

[Histonet] IHC/Special staining

Does anyone know of any lab with excess capacity good quality and fast turnaround that could do IHC/special staining on the side? We are out of capacity, and we don't always have the antibodies that we need. Kevin kdea...@hotmail.com ___ Histonet

[Histonet] IHC CRO

Hello, Does anyone has a list of category can share that is used for IHC CRO comparison? I want to make a table to do comparison after talking to multiple CROs and I don't want to miss something. Just wondering if anyone did this before and can share your table or list? Thanks, Wendy

[Histonet] IHC wash buffer temperature?

Hello Histonetters, I work in research and we get too much non-specific background on our IHC-s. Anybody tried using wash solution at 37C instead of at room temperature? If not, why? Is it a bad idea? Thanks, Hanna Preiszner ETSU Pathology ___ Histonet

[Histonet] IHC QC Forms

Hi, I will be glad if you guys can share your IHC QC forms with me. Sincerely, Adesupo A ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC gunk on slides

gunk (Willis, Donna G) >3. Re: IHC gunk (Thomas Podawiltz) >4. Re: IHC gunk (Mac Donald, Jennifer) >5. Re: Histonet Digest, Vol 232, Issue 7 (Eddie Martin) > > > > -- Forwarded message -- > From: Karen Heckford CA-San Francisco > To: Histonet &g

Re: [Histonet] IHC gunk

Is it possible that it is hematoxylin precipitate? -Original Message- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 10:45 AM To: Histonet Subject: [Histonet] IHC gunk EXTERNAL SENDER - Exercise caution with requests, links, and attachments

Re: [Histonet] IHC gunk

University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -Original Message- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 12:45 PM To: Histonet Subject: {EXTERNAL} [Histonet] IHC gunk CAUTION

Re: [Histonet] IHC gunk

Francisco via Histonet Sent: Wednesday, March 22, 2023 12:45 PM To: Histonet Subject: {EXTERNAL} [Histonet] IHC gunk CAUTION:  This email originated outside of BSWH. The actual sender is (histonet-boun...@lists.utsouthwestern.edu) which may be different from the display address in the From

[Histonet] IHC gunk

Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may

Re: [Histonet] IHC staining of tendons and cartilage

Riley Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC staining of tendons and cartilage Hi Charles, Shirley Powell here in humid Georgia. I ran an IHC reference lab here for many years. I had a problem with using charged slides for a lot of the tissues I processed. I used

Re: [Histonet] IHC staining of tendons and cartilage

Phone: 478-301-2374 https://medicine.mercer.edu/ -Original Message- From: Charles Riley via Histonet Sent: Tuesday, March 21, 2023 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining of tendons and cartilage Hello all, I am in a new position

[Histonet] IHC staining of tendons and cartilage

Hello all, I am in a new position and it will potentially require doing a lot of IHC testing on cartilage and tendon samples. I have done some practice runs on my automated stainer and manually and am running into issues with the tissue sections falling off completely or folding over on itself

[Histonet] IHC systems

Dear all We are planning to purchase a new IHC system. May I request your valuable feedback, observation and troubleshooting encountered while handling Leica Bond 3? Would you prefer Leica or Roche for the quality of staining, consumables supply and user-friendly lean management with help in

[Histonet] IHC position in Gainesville FL

Hi Histonetters, Just wanted to let everyone know I am looking for an histotechnologist to work in the IHC area of the Histology lab here at UF Health Shands hospital. I am looking for an a person that is energetic, hardworking and willing to learn all aspects of the immunohistochemistry area.

Re: [Histonet] IHC background

你*** ---原始邮件--- 发件人：人生过客$ via Histonet"http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC background

？？？ ---原始邮件--- 发件人：人生过客$ via Histonet"http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC background

你理我不 ---原始邮件--- 发件人：Tony Henwood (SCHN) via Histonet"http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC background

Paula, What does the artefact look like? Another cause could be partial lifting of parts of the section, allowing reagents to become trapped under the section. You commonly see it in thyroids where the colloid traps antibody and/or detection reagents under the colloid giving a particulate

Re: [Histonet] IHC background

- From: Regan Fulton via Histonet Sent: Thursday, September 29, 2022 3:42 PM To: RENE MORALES Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC background *** This is an External Email *** Hi, The artifact you are seeing is a well-known artifact of the Leica covertile design

Re: [Histonet] IHC background

Hi, The artifact you are seeing is a well-known artifact of the Leica covertile design. It does limit the available footprint for placing samples. Hope this helps. Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360

Re: [Histonet] IHC background

Paula, Regardless of the position of the tissue: control/ patient ‘s tissue, most likely is the distribution of the reagents during staining, isn’t adequate. Syringe, staining reagents expiration, calibration, etc., Have you contacted service from Leica? Hope the solution is found, Thanks,

[Histonet] IHC background

Hello everyone, We have the Bond III and we ran an S100. We placed 2 serial sections vertically on the slide with the control tissue at the top for staining. The patient's section at the top underneath the control tissue had background staining, while the section on the bottom of that had no

[Histonet] IHC staining of cartilage

We use P8920 Sigma-Aldrich Poly-L-lysine solution. Great retention without excess background staining. You can prep a rack of slides in diluted solution but we put a gloved finger over the end of the bottle and and swipe it down the slide at full strength. Let the slide dry and move forward as

Re: [Histonet] IHC staining of cartilage

Sent: Tuesday, March 01, 2022 1:30 PM To: histonet Subject: Re: [Histonet] IHC staining of cartilage WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Yes

Re: [Histonet] IHC staining of cartilage

Hi, With regard to adhesion, we find TOMO slides are the best among many that we have tried. Interestingly, a creative histotech I once worked with used a diluted Gorilla Glue smear on the slides just prior to picking up a cartilage section. It was tricky to get the section on while the glue

Re: [Histonet] IHC staining of cartilage

Yes...a big problem for me, tooif you are using HIER?? Cartilage and bone are major tissue retention problems I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC I still get a % fall off but, using manual HIER at 90C I manage to get enough sections to get good results

[Histonet] IHC staining of cartilage

I recently tried running an automated ihc test on a few pieces of cartilage tissue. All of the sections fell off my slides. I am using the TOMO charged slides and have never had any issues. Do cartilage sections require longer drying times in order for the tissue to adhere better?

[Histonet] IHC Research Technician Kansas State Veterinary Diagnostic Lab

Hello HistoNetters, The Kansas State Veterinary Diagnostic Laboratory (KSVDL) within the College of Veterinary Medicine is seeking applicants for a Research Assistant. This position will have the primary responsibility of coordinating new incoming research projects and developing new tests

Re: [Histonet] IHC with zamboni's fixed tissues

onet@lists.utsouthwestern.edu] Sent: Friday, 26 March 2021 9:42 AM To: histonet-request Subject: [Histonet] IHC with zamboni's fixed tissues Hello Histonet, Can you give me feedback on trying to do IHC on samples fixed in Zamboni's fixative? I have an idea but need confirmation . Thank you in advance Col

[Histonet] IHC with zamboni's fixed tissues

Hello Histonet, Can you give me feedback on trying to do IHC on samples fixed in Zamboni's fixative? I have an idea but need confirmation . Thank you in advance Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455

[Histonet] IHC Disclaimer for immunofluorescence stains

Those labs doing diagnostic immunofluorescence for kidney or skin, do you put in the IHC disclaimer used for peroxidase staining on the reports? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

Re: [Histonet] IHC validation after a new tissue processer is installed

Hi martha, Prognostic markers must be re-validated (Eg.s Breast markers and CD117) as you described. Every Ab in your menu should be tested (as you would for a new a new lot) and do not forget to validate your H (with various tissue types) and SS as well. For the H, if possible do side-by-side

[Histonet] IHC validation after a new tissue processer is installed

Hello all, After a very long time our histology department is getting a new tissue processor, and hopefully several more in the near future. Of course for IHC that means a revalidation/reverification of our IHC stains. Since I have not had to do this before I wanted to get some guidance on

Re: [Histonet] IHC validations

2020 09:25:03 -0700 From: "Paula" To: Subject: [Histonet] IHC validations Hello, I see 10 positive and 10 negative cases for CAP guidelines, but what about for CLIA? What is their guideline to validate an IHC antibody? Thanks in adva

Re: [Histonet] IHC validations

Paula: Usually CLIA follows CAP's lead or has less strict guidelines. Following the CAP guidelines should be good for both. Claire From: Paula via Histonet Sent: Tuesday, June 2, 2020 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC

[Histonet] IHC validations

Hello, I see 10 positive and 10 negative cases for CAP guidelines, but what about for CLIA? What is their guideline to validate an IHC antibody? Thanks in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] IHC validation

involved in procedure validation, but this is my attempt to answer your initial question. Joe Myers, M.S., CT/QIHC(ASCP) *** Message: 2 Date: Wed, 6 May 2020 05:43:22 -0700 From: Kristy Castillo To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC validation Would like

[Histonet] IHC validation

Would like to know when validating for IHC for a dermatology lab and just for CLIA (no CAP), do you just need to show a shave, punch and excision lighting up? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] IHC control blocks, DAB kit

Kristy, this might be of some help too: https://www.fixationonhistology.com/post/control-tissue-faqs [https://static.wixstatic.com/media/128854_2fda1d8f8d5c48cdad7701b3e866d885~mv2.jpg/v1/fit/w_1000,h_728,al_c,q_80/file.png] Control

Re: [Histonet] IHC DAB

Research Assistant/Laboratory Manager Atherothrombosis and Vascular Biology Laboratory From: Joe Myers Sent: Saturday, 2 May 2020 4:49 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC DAB Kristy: I’m thinking along the same lines as Paula

Re: [Histonet] IHC DAB

of the antigen, pretreatment reagent and ‘labeling’ enzyme. Cheers, Joe Myers, M.S.,CT/QIHC(ASCP) From: Kristy Castillo via Histonet Sent: Friday, May 1, 2020 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Hi Histonetters, We are starting

Re: [Histonet] IHC DAB

Since you are newbies, I am just putting IHC 101 out there.  Permanent red is for alkaline phosphatase detection and DAB is for HRP. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp

Re: [Histonet] IHC DAB

Thanks for the tip! On Fri, May 1, 2020 at 8:53 AM Val Tolley wrote: > It’s difficult to know without your full protocol, but you can easily test > your DAB. > > Take a paper towel and add a drop of your secondary/HRP. Add a drop of > DAB to that. If it doesn’t immediately turn brown, your

Re: [Histonet] IHC DAB

It’s difficult to know without your full protocol, but you can easily test your DAB. Take a paper towel and add a drop of your secondary/HRP. Add a drop of DAB to that. If it doesn’t immediately turn brown, your hydrogen peroxide has likely degraded into water. Make a fresh batch of DAB and

Re: [Histonet] IHC DAB

Hydrogen Peroxide gone bad / or not being added??? From: Kristy Castillo via Histonet Sent: Friday, May 1, 2020 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Hi Histonetters, We are starting our IHC (fun times), we are having

[Histonet] IHC DAB

Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy ___ Histonet mailing list

Re: [Histonet] IHC troubleshooting

Nancy, You used the word “blot” in your reply to Charles, and while I can’t be certain that you did not mean the same...I would like say in the interest of clarity, that it is safer to carefully “wick” the water away from under each section prior to baking. Blotting (like we might do after a Gram

[Histonet] IHC question

I have a question regarding ihc I’m running some ihc using Dako linker 48, this gave me a notice of short reagent but when I made it my time was off and suspended. The slides are just being on peroxide block for 5 min. Can I just reload them again? Is this will not affect my staining Ir what is

[Histonet] IHC Troubleshooting and Water Baths

To: Histo List Subject: [Histonet] IHC troubleshooting EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone

Re: [Histonet] IHC troubleshooting

onet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, February 26, 2020 9:44 AM To: Histo List Subject: [Histonet] IHC troubleshooting EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Our pathologists are complaining that chunks of the dermis are mis

Re: [Histonet] IHC Troubleshooting

Hi Charles, It adhesion problems can arise from several sources, some have already been mentioned. Here are two that I have experienced: 1) Bad slides. Either a manufacturing defect such that the positive charge is insufficient or the slides were somehow compromised after opening them on the bench

Re: [Histonet] IHC troubleshooting

In my experience; If the the specimen is under-fixed or under-processed, the section will be sloughed off during IHC staining. I find proper fixation time and drying the slides at room temperature (before baking them in oven) optimal. Andy Abdulbasit Andijany (Andy) AMLS, BBMS, MSc, CT

Re: [Histonet] IHC troubleshooting

Do you float and pick up your sections from a water bath of distilled water with no adhesive added? If not, doing that will help. John On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet wrote: > Our pathologists are complaining that chunks of the dermis are missing from > IHC slides

[Histonet] IHC troubleshooting

Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. --

Re: [Histonet] IHC on Pig Tissues

erlab.com/> Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Lori Sorrells via Histonet Sent: Wednesday, January 29, 2020 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Pig Tissues Good afternoon- Has anyone run into problems

[Histonet] IHC on Pig Tissues

Good afternoon- Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers

[Histonet] IHC Application Specialist Job in Boston-

Hello and Happy Monday, I have an "off the bench" position available for an IHC Application Specialist. Looking for someone with extensive IHC experience and a research emphasis rather than clinical emphasis on experience. Bachelor's Degree at least. Ability to travel throughout Boston and the

Re: [Histonet] IHC mouse optomization

Subject: Re: [Histonet] IHC mouse optomization Biocare Medical has pure ready to use secondary.. Naira -Original Message- From: Blanca Lopez Sent: Wednesday, June 12, 2019 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC mouse optomization Morning, I need a good

Re: [Histonet] IHC mouse optomization

Biocare Medical has pure ready to use secondary.. Naira -Original Message- From: Blanca Lopez Sent: Wednesday, June 12, 2019 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC mouse optomization Morning, I need a good advice in optimizing Mouse antibodies

Re: [Histonet] IHC mouse optomization

Biocare Medical Promark series are also great animal polymer based detection that work very well. https://biocare.net/products/detection/ Colleen Forster HT(ASCP)WQIUC U of MN On Wed, Jun 12, 2019 at 10:42 AM Paula Keene Pierce via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Vector

Re: [Histonet] IHC mouse optomization

Vector Labs ImmPress kits rock for animal IHC. https://vectorlabs.com/browse/immpress-polymer-detection-kits-for-ihc Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Wednesday, June

[Histonet] IHC mouse optomization

Morning, I need a good advice in optimizing Mouse antibodies for research purposes. I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit HRP has a mixture of mouse and rabbit so we think that might be the reason become negative. Is there any tips for optimizing

[Histonet] IHC Specialists Needed CA, IL, AZ and New England.

Hi Histonetters! Just a quick note about some exciting new opportunities. I am recruiting for IHC Specialists nationwide. I have a number of roles ranging from in-house to field applications specialists. If you are interested please shoot me a quick email for details. And. If you refer someone

Re: [Histonet] IHC Control Slide Longevity

Hi Sandy, We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same morning as staining; case material as well). We've never had any problems with Synaptophysin; it's always worked very well on unstained controls that are up to a year old (stored in the fridge). Best regards, Jan

[Histonet] IHC Control Slide Longevity

Hello all, Do any of you have to cut any of you antibody controls "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We seldom do a Syanpt., and have found the fresh cut patient tissue has been positive while the not-so-fresh positive control slide is negative.

Re: [Histonet] IHC Billing - 88342 vs. 88341

- From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, January 21, 2019 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] IHC Billing - 88342 vs. 88341 For those of you doing clinical IHC testing, have you been able to automate

Re: [Histonet] IHC Billing - 88342 vs. 88341

Medical (914) 302-8424 ap...@cmmedical.com -Original Message- From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, January 21, 2019 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] IHC Billing - 88342 vs. 88341 For those

[Histonet] IHC Billing - 88342 vs. 88341

For those of you doing clinical IHC testing, have you been able to automate the billing of IHC (88342 vs. 88341) or do you still manual bill? Our IT staff wants to build-in a "88342" and a ""88341" for every antibody that we run and then have the pathologist select the appropriate one for

Re: [Histonet] Histonet - IHC Manual staining

Hello Dave, I still do a lot of manual work and I also use Biocare reagents. They work well and their customer service is very good. Colleen Forster On Fri, Jan 18, 2019 at 12:58 PM Jeanine Ronkowski via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Dave:Our lab has used reagents

[Histonet] Histonet - IHC Manual staining

Hi Dave:Our lab has used reagents from Biocare Medical for a very long time. They’re packaged in 6ml and 25 ml vials and ideal for hand staining. And their technical support is great tooJeanine Hello Histoneters - Does anyone still do manual IHC's? If so, which IHC staining kits would you

[Histonet] IHC Manual Staining

Hello Histoneters - Does anyone still do manual IHC's? If so, which IHC staining kits would you recommend? Thanks. Yours, Dave ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC-H HELP...

Hi Curt, You can de-stain the hematoxylin counter stain and then do H and all should be fine. Subsequent IHC is possible but obviously you would need a different color chromogen to differentiate the new stain from the previous one. There may be a problem getting the section stay on through a

Re: [Histonet] IHC-H HELP...

January 2019 4:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC-H HELP... Need help before I turn a mistake into an irreparable mistake... We have some unstained slides that were supposed to get stained with H but my guy stained them with IHC. It's complicated, we receive

[Histonet] IHC-H HELP...

Need help before I turn a mistake into an irreparable mistake... We have some unstained slides that were supposed to get stained with H but my guy stained them with IHC. It's complicated, we received slides and a block, the block was for IHC, the unstained slides were for H, he inverted the

Re: [Histonet] IHC Negative reagent controls

The article referenced below may be of interest. Standardization of Negative Controls in Diagnostic Immunohistochemistry: Recommendations From the International Ad Hoc Expert Panel

Re: [Histonet] IHC Negative reagent controls

4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Cayman Fleck via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 3 January 2019 1:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Negative reagent controls A question that came up

[Histonet] IHC Negative reagent controls

A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well

[Histonet] IHC control slides for EBNA

Does anyone know of a source of IHC control slides for EBV Nuclear Antigen? Thank you. Michele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC Stainer

ge- > From: Sandra Cheasty [mailto:sandra.chea...@wisc.edu] > Sent: Thursday, August 30, 2018 9:40 AM > To: Histonet (histonet@lists.utsouthwestern.edu) utsouthwestern.edu> > Subject: [Histonet] IHC Stainer > > Hello all, > We are in the market for a new IHC stainer. (O

Re: [Histonet] IHC Stainer

as well, instead of in the pressure cooker, but I haven't seen it in action yet. -Original Message- From: Sandra Cheasty [mailto:sandra.chea...@wisc.edu] Sent: Thursday, August 30, 2018 9:40 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Stainer Hello all

Re: [Histonet] IHC Stainer

Get a DAKO IHC stainer (even if it is "refurbished")René On Thursday, August 30, 2018 9:56 AM, Sandra Cheasty via Histonet wrote: Hello all,                 We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but

[Histonet] IHC Stainer

Hello all, We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides. Not interested in a closed system; it is for a veterinary histology lab, and we need the

[Histonet] IHC System from StatLab

I’m pleased to report that I was finally contacted by a representative from StatLab that provided me with the information I was seeking.  I realize that their system may not be fully released, but I didn’t think it would be that difficult for them to provide information.  Turns out that this

Re: [Histonet] IHC System from StatLab

All:For those of you who saw my posting last week and we’re wondering about the outcome, I’m sorry to say that I’ve received very little useful feedback.  Two individuals from StatLab sent me separate, cryptic messages indicating that they would be be happy to either meet with me or provide a

[Histonet] IHC System from StatLab

I’ve heard from a number of people that Stat Lab plans to release a new instrument for immunostaining and I was wondering if someone, including the folks at StatLab, might be able to provide more information.Thx,Jeanine Sent from Yahoo Mail for iPhone

[Histonet] IHC question

Does anyone run this antibody on their Leica bond platforms? BRAF V600E (VE1)? I am looing to purchase it from sigma aldrich ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Symposium in Quebec City

2018 cIQc Symposium: IHC Toolkits for Diagnostic Pathology Where: Quebec City at the Hotel Palace Royale (775 Boulevard Honoré-Mercier) When: September 20-21, 2018. Go to www.ciqc.ca for more information. The symposium is for Lab Scientists, Pathologists and

Re: [Histonet] IHC and Histogel

We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference

Re: [Histonet] IHC and Histogel

, May 10, 2018 10:20 AM To: Paula; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and Histogel We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet <histo

[Histonet] IHC and Histogel

Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] IHC validation

, this should be CAP compliant. Cae Aguilar, HTL (ASCP) Histology Supervisor 7079802801 -Original Message- From: Allan Wang [mailto:all...@biomax.us] Sent: Thursday, March 22, 2018 11:02 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC validation How are labs validating for rarer

[Histonet] IHC Platforms

Hello everyone. I was wondering if any small, GI labs do IHC's in house and if so what platforms you use. I am just researching right now to see if it would be worth it to do in house or not. I know a lot of these instruments and reagents are expensive so I would be looking for the most

[Histonet] IHC protocol help

Is anyone out there using the Leica Bond III and Bond Max to perform the following tests? ACD RNA ISH Probe EBV from Leica CDK4 and or MDM2 from ABCAM Amyloid P from Biocare Amyloid A from Epitomics I am trying to optimize and validate them our on machines and would like some help where to

  1   2   3   4   5   6   7   8   >