Re: [galaxy-user] FASTQ type change

2011-02-17 Thread Peter Cock
On Thu, Feb 10, 2011 at 6:42 PM, Peter wrote: On Thu, Feb 10, 2011 at 6:34 PM, Stephen Taylor wrote: Sounds like this should be added into the main release. It would save a lot of time/disk space instead of using Groomer. I agree. Maybe Dan can take care of it - I don't have bowtie setup

Re: [galaxy-user] Group and hide steps in history/workflow

2011-02-22 Thread Peter Cock
On Tue, Feb 22, 2011 at 1:23 PM, Felix Hammer hamm...@cip.ifi.lmu.de wrote: I've done that. The intermediate outputs are hidden. But all the steps are still displayed. I want to hide those, too. Felix I'm unclear which bit(s) you want to hide in the GUI. With the recent enhancements, the run

Re: [galaxy-user] Quality Score

2011-02-24 Thread Peter Cock
On Thu, Feb 24, 2011 at 8:47 PM, Amy Boddy abo...@med.wayne.edu wrote: Hello, I was wondering how Galaxy calculates the quality score for NGS data? Is there any documentation I could read on how this is calculated? Thanks! -Amy Boddy Which quality score are you talking about? Raw reads?

Re: [galaxy-user] Quality Score

2011-02-28 Thread Peter Cock
Great :) Don't forget to CC the mailing list in future ;) On Fri, Feb 25, 2011 at 4:46 PM, Amy Boddy abo...@med.wayne.edu wrote: Thank you, these articles helped! On Thu, Feb 24, 2011 at 5:47 PM, Peter Cock p.j.a.c...@googlemail.com wrote: On Thu, Feb 24, 2011 at 8:47 PM, Amy Boddy abo

Re: [galaxy-user] Is there any tool in Galaxy for trimming the Barcode in 454 sequencing data? Thanks.

2011-02-28 Thread Peter Cock
On Mon, Feb 28, 2011 at 9:28 PM, Yang Liu ywl5...@psu.edu wrote: Is there any tool in Galaxy for trimming the Barcode in 454 sequencing data? Thanks. Hi, The Roche off instrument applications cover this (working directly with the SFF files). Try asking Edward Kkirton if he would update his

Re: [galaxy-user] local directory

2011-03-12 Thread Peter Cock
On Fri, Mar 11, 2011 at 10:07 PM, Albert Ayoub albert.ay...@yale.edu wrote: I installed Galaxy on my own linux machine. I would like to perform analysis on local RNAseq files since they are too big to upload. How do we add files from a local directory or a network share? Providing you are a

Re: [galaxy-user] FASTQ to FASTQSanger using Groomer question

2011-03-21 Thread Peter Cock
On Mon, Mar 21, 2011 at 1:42 PM, David K Crossman dkcro...@uab.edu wrote: Hello!     I am fairly new to using Galaxy and have a question about the FASTQ Groomer feature.  I have 4 RNA-Seq raw data files that were just recently generated from Illumina’s NGS instruments. Very

[galaxy-user] Combining tables with different numbers of columns

2011-04-13 Thread Peter Cock
Hi all, I'm having a little trouble understanding the best way to perform some tabular file manipulations in Galaxy. I have several tabular files, which contain different numbers of columns, which I want to combine using a single column containing an identifier (which must match for the rows to

Re: [galaxy-user] BAM files

2011-04-20 Thread Peter Cock
On Wed, Apr 20, 2011 at 4:00 AM, Edward Dudley egd...@psu.edu wrote: Hi - I have a set of 454 reads that have been trimmed and converted to BAM format using Galaxy, and I can visualize the alignment with E. coli genomes using the UCSC browser.  Problem is, I'd like to display the alignment in

Re: [galaxy-user] quality stat.

2011-05-03 Thread Peter Cock
On Tue, May 3, 2011 at 10:00 AM, Robin Mjelle robinmje...@gmail.com wrote: Dear User, I am trying to use the tool Compute quality statistics in galaxy on Ilumina single reads. The file is 2.3 Gb, fastq format. I have performed Quality format converter on the data set and the format is now

Re: [galaxy-user] Text Manipulation: Filter out duplicates (uniq) from an plain text file ?

2011-05-06 Thread Peter Cock
On Fri, May 6, 2011 at 3:16 PM, Roman Valls brainst...@nopcode.org wrote: Well, having similarly basic tools (in Galaxy) that can be performed on the commandline, such as sort or cut I just wondered how come a uniq is not there on the tool panel in some form/name. Thanks for the feedback Rory

Re: [galaxy-user] (no subject)

2011-05-24 Thread Peter Cock
On Tue, May 24, 2011 at 12:40 AM, Aaron Jex a...@unimelb.edu.au wrote: Hi, Can’t seem to find an answer to this on your wiki site and it’s not in the tutorial.  I would like to filter my 454 reads for high quality regions, rename the resulting sequence fragments AND relink the new reads

Re: [galaxy-user] suggestion for multithreading

2011-06-02 Thread Peter Cock
2011/6/2 Nate Coraor n...@bx.psu.edu: Hi Louise-Amélie, I haven't done anything with this code yet, but I wanted to let you know that we'll eventually be adding it, I'm just going to change the implementation slightly.  I'd like to merge the functionality of the csv into an xml config I'm

Re: [galaxy-user] FTP and command line access in Galaxy

2011-06-22 Thread Peter Cock
On Wed, Jun 22, 2011 at 6:47 AM, Robert Curtis Hendrickson curt...@uab.edu wrote: Nate, The Galaxy’s ability to pull files with user/password from FTP sites as a client is great. However, I need to pull data from an HTTP site at a sequencing center with user/password (already tried to get

Re: [galaxy-user] blastn: NameError: name 'cmd' is not defined

2011-07-02 Thread Peter Cock
On Fri, Jul 1, 2011 at 4:35 PM, Sergei Ryazansky s.ryazan...@gmail.com wrote: Thanks for answer, but I have just updated the galaxy source code and blastn works fine! Sorry for any inconvenience :) It was an error in the wrapper script's error handler, already fixed. Peter

Re: [galaxy-user] BAM files loading and mandatory grooming

2011-07-27 Thread Peter Cock
2011/7/27 Louise-Amélie Schmitt louise-amelie.schm...@embl.de: Hello Jennifer, Aaaah so the grooming means creating the .bai file? Ok, I didn't know, thanks for the information. But that leaves me wondering why it is necessary to copy the data (unless the alignments are not sorted yet, which

Re: [galaxy-user] mi-tools- tools_fabfile.py permissions requirements (local install)

2011-08-01 Thread Peter Cock
Note: Local install questions are normally directed to the galaxy-dev list (CC'd) rather than galaxy-user On Mon, Aug 1, 2011 at 3:21 PM, Joseph Hargitai joseph.hargi...@einstein.yu.edu wrote: Enis, a few things we came across: a, local install R - not the correct download url, Which URL

Re: [galaxy-user] running cufflinks

2011-09-07 Thread Peter Cock
On Tue, Sep 6, 2011 at 9:29 PM, Peng, Tao tp...@fhcrc.org wrote: Hi I had 3 GTF files from ensemble, UCSC and NCBI for annotation; ONLY ensemble were recognized by GALAXY cufflinks as a GTF file although they all have .GTF. I am NOT sure why UCSC and NCBI GTF files were seen as GFF files?

Re: [galaxy-user] Installing Galaxy on my computer

2011-09-12 Thread Peter Cock
On Mon, Sep 12, 2011 at 5:20 PM, Lilach F lilac...@gmail.com wrote: Hi Jennifer, I already tried 2 weeks ago to upload my whole exome data by FTP, according to the instructions you sent below. The files are too big, it should take days for each file, and it always stops in the middle after 12 

Re: [galaxy-user] Getting (or setting) physical file name

2011-09-15 Thread Peter Cock
On Thu, Sep 15, 2011 at 6:11 PM, Paul-Michael Agapow paul-michael.aga...@hpa.org.uk wrote: So one of my colleagues has a script he wants to turn into a  Galaxy tool. The twist is that script: 1.   Looks for files with a fixed name (e.g. “params.txt”) 2.   Accepts other file names

Re: [galaxy-user] Galaxy and Ion Torrent

2011-09-28 Thread Peter Cock
2011/9/28 cuentasecuenciacion secuenciac...@ipb.csic.es: Hello!! Does anybody know whether Ion Torrent data is supported by Galaxy? Thank you!! IonTorrent data acts a lot like Roche 454, it even comes as SFF files (or FASTQ). So in principle anything Galaxy supports for 454 would be worth

Re: [galaxy-user] Patch for better FASTQ description handling

2011-10-19 Thread Peter Cock
On Wed, Oct 19, 2011 at 4:53 AM, Florent Angly florent.an...@gmail.com wrote: I have had the chance to try the patch on several datasets and it looks good :) I reiterate my suggestion to pull the patch in galaxy-central. Best, Florent It looks sensible, although I would add a comment to the

Re: [galaxy-user] Patch for better FASTQ description handling

2011-10-20 Thread Peter Cock
On Thu, Oct 20, 2011 at 2:15 PM, Eric Cabot ca...@biotech.wisc.edu wrote: I was not aware of this new naming. It seems like a terrible decision from Illumina because now both reads in a pair technically have the same ID (but a different description). This is not quite the case. Here are two

Re: [galaxy-user] tool.xml console redirection within command-tag

2011-10-24 Thread Peter Cock
On Mon, Oct 24, 2011 at 9:01 AM, Appelt, Uwe uwe.app...@nct-heidelberg.de wrote: Dear all, that has probably been asked 10e6 times, however, I don't find any comments on how to properly escape special characters in a toolxy.xml. What I want to achieve is the following: command

Re: [galaxy-user] tool.xml console redirection within command-tag

2011-10-24 Thread Peter Cock
On Mon, Oct 24, 2011 at 9:42 AM, Appelt, Uwe uwe.app...@nct-heidelberg.de wrote: Hi Peter, thanks a lot for your quick reply. However amp;gt; doesn't seem to work as well. Also, I do have to admit that two different doesn't work exist. Notations that are not accepted during parsing of

Re: [galaxy-user] tool.xml console redirection within command-tag

2011-10-24 Thread Peter Cock
Hi Uwe, On Mon, Oct 24, 2011 at 10:47 AM, Appelt, Uwe uwe.app...@nct-heidelberg.de wrote: Hi again, yes, you're right. The problem source is something else. As soon as I specify one of the following command interpreter=bash$hisapWrapperScript amp;gt; $logFile/command command

Re: [galaxy-user] Problem with bam and/or bai files

2011-10-27 Thread Peter Cock
Sending to galaxy-dev ... On Thu, Oct 27, 2011 at 5:51 AM, Jim Robinson jrobi...@broadinstitute.org wrote: Hi Mike, Someone from the Galaxy team can perhaps give some insight on what went wrong,  I can comment on the error message from IGV. That error is thrown from Picard, in every case

Re: [galaxy-user] fastq groomer

2011-11-01 Thread Peter Cock
On Tue, Nov 1, 2011 at 4:58 PM, Kevin Lam abou...@gmail.com wrote: actually Illumina 1.8+ has one more quality value higher than fastqsanger (see http://en.wikipedia.org/wiki/FASTQ_format ) my question now I guess is if I use fastqsanger would it break anything when it encounters the 'J' in

Re: [galaxy-user] selecting reads at random from fastq file

2011-11-08 Thread Peter Cock
On Tue, Nov 8, 2011 at 9:57 PM, Austin Paul austi...@usc.edu wrote: Hi, I am curious if anyone knows how to select random lines from a fastq file. There is a select random lines tool in text manipulation tools, but it does not treat fastq files specifically, so it will not group quality lines

Re: [galaxy-user] selecting reads at random from fastq file

2011-11-08 Thread Peter Cock
On Tue, Nov 8, 2011 at 10:26 PM, Austin Paul austi...@usc.edu wrote: Hi Peter, Thanks for the suggestion.  For example, I have a fastq file with 50 million reads and I want to randomly select 5 million of them. It seems biopython would very easily select a single or a handful of reads with

Re: [galaxy-user] Question about file formats

2011-11-10 Thread Peter Cock
On Thu, Nov 10, 2011 at 6:10 PM, Rena Zheng rzh...@mail.med.upenn.edu wrote: Hi, I uploaded a bed file to Galaxy and did some text manipulations.  I want to download the new file as a bed format that I can then open up in excel or a text editor.  However, when I save the data, it is a .tabular

Re: [galaxy-user] https://galaxy.jgi-psf.org/

2011-11-14 Thread Peter Cock
On Mon, Nov 14, 2011 at 10:08 PM, Chauhan, Archana achau...@utk.edu wrote: Dear Sir/Madam,     I am registered with galaxy http://main.g2.bx.psu.edu/. But I recently came across another link as  https://galaxy.jgi-psf.org/ . This has some very good applications especially

Re: [galaxy-user] EMBOSS fuzzpro tool pattern is not read correctly from the input

2011-12-12 Thread Peter Cock
On Mon, Dec 12, 2011 at 4:34 PM, domantas.motieju...@cropdesign.com wrote: Hello, I'm trying to run EMBOSS fuzzpro tool, however, I get Illegal character error '_', aparently from the fuzzpro tool itself. One of the input parameters is amino acid sequence pattern, for instance I submit

Re: [galaxy-user] How can I tell what programs are used by a tool?

2012-02-07 Thread Peter Cock
On Tue, Feb 7, 2012 at 9:52 PM, Beale, Holly (NIH/NHGRI) [F] holly.be...@nih.gov wrote: Hi all -- I'd like to know what programs are used when I run a tool on my data. Ideally I'd also like to know what parameters are passed to command line tools. The short answer is you can't, other than by

Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Peter Cock
On Thursday, February 9, 2012, Jennifer Jackson j...@bx.psu.edu wrote: Hi David, It sounds like you are using the public Galaxy server at http://usegalaxy.org (http://main.g2.bx.psu.edu)? A BLAST service is not available on the public instance. (Megablast is available, but for NGS query data

Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-10 Thread Peter Cock
On Thu, Feb 9, 2012 at 9:57 PM, Jennifer Jackson j...@bx.psu.edu wrote: Hi Peter, It looks to me like there are versions both, with the Tool Shed version having the more recent date stamp. But if those extra functions are not needed, then yes, you are correct, the version included in the

Re: [galaxy-user] Large local file of NGS for FASTAQ Groomer

2012-02-12 Thread Peter Cock
On Sun, Feb 12, 2012 at 10:28 PM, Arthur Zheng haoz...@gmail.com wrote: Hi, I have downloaded and installed a local instance of galaxy on the linux server using my user account according to here: http://main.g2.bx.psu.edu/ Then I ran the following command: sh run.sh and accessed galaxy

Re: [galaxy-user] Large local file of NGS for FASTAQ Groomer

2012-02-13 Thread Peter Cock
On Mon, Feb 13, 2012 at 2:17 AM, Arthur Zheng haoz...@gmail.com wrote: On Sun, Feb 12, 2012 at 4:34 PM, Peter Cock p.j.a.c...@googlemail.com wrote: On Sun, Feb 12, 2012 at 10:28 PM, Arthur Zheng haoz...@gmail.com wrote: Hi, I have downloaded and installed a local instance of galaxy

Re: [galaxy-user] wrapper question prefix for output files.

2012-02-23 Thread Peter Cock
On Thu, Feb 23, 2012 at 5:46 PM, Victor Ruotti ruo...@wisc.edu wrote: Hi, I hope someone can help me on how to implement this into a wrapper. This kind of question is normally redirected to the galaxy-dev list. We would like to add an option so the user can set a sample name which then be

Re: [galaxy-user] [galaxy-bugs] GI errors in the megablast table of results ?

2012-03-01 Thread Peter Cock
Hello all, Did this issue get resolved? If Sandrine was right about there being an off by one error in GI number in the BLAST tabular output, it could be a bug in 'legacy' blastall command. I say 'legacy' BLAST because that's what Galay's NGS 'megablast' tool is using internally (as opposed to

Re: [galaxy-user] Galaxy unable to set metadata for GFF files

2012-03-04 Thread Peter Cock
On Sun, Mar 4, 2012 at 6:34 PM, Hemarajata, Peera hemar...@bcm.edu wrote: Dear all, I’m been trying to get Galaxy to recognize this GFF from NCBI ( ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Lactobacillus_reuteri_JCM_1112_uid58875/NC_010609.gff) but it failed to recognize the format after I

Re: [galaxy-user] Metagenomics

2012-03-12 Thread Peter Cock
On Mon, Mar 12, 2012 at 6:28 PM, John Major john.e.major...@gmail.com wrote: A small warning re-the current cloud-Blast+ config. To properly use the metagenomic tools, if you use the blast+ galaxy tool, make sure to export in blast.XML, then you'll need a script to parse out the readID and

Re: [galaxy-user] Combining two workflows into one

2012-03-19 Thread Peter Cock
You forgot to CC the mailin list. On Mon, Mar 19, 2012 at 12:02 PM, Peter Cock p.j.a.c...@googlemail.com wrote: On Mon, Mar 19, 2012 at 4:53 PM, Innocent Onsongo onson...@umn.edu wrote: Galaxy Team, I would like to create a workflow by combining portions of two different workflows. How do

Re: [galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Peter Cock
2012/3/21 Makis Ladoukakis makis4e...@hotmail.com: Dear Galaxy users, I have been trying to upload a blastable database in my local instance of galaxy. I have used the nr database and generated all the nhr, nin, nsq, and nal files. I have also edited the blastdb.loc file in the

Re: [galaxy-user] Trouble with BLAST+ and .loc file

2012-03-21 Thread Peter Cock
On Wed, Mar 21, 2012 at 10:21 AM, Peter Cock p.j.a.c...@googlemail.com wrote: 2012/3/21 Makis Ladoukakis makis4e...@hotmail.com: Dear Galaxy users, I have been trying to upload a blastable database in my local instance of galaxy. I have used the nr database and generated all the nhr, nin, nsq

Re: [galaxy-user] Blast2GO local instance Re: Table with gene count reads

2012-04-03 Thread Peter Cock
On Mon, Apr 2, 2012 at 6:41 PM, Greg Von Kuster g...@bx.psu.edu wrote: On Mar 24, 2012, at 7:30 AM, Peter Cock wrote: Have you seen the README file that comes with the Blast2GO wrapper? Perhaps the 'install from toolshed' could be tweaked to make this kind of documentation more visible

Re: [galaxy-user] MegaBLAST output

2012-04-24 Thread Peter Cock
On Tue, Apr 24, 2012 at 10:24 PM, Jennifer Jackson j...@bx.psu.edu wrote: ..., using the BLAST+ BLASTN megablast wrapper that Peter authored, in a local or cloud instance, would be the best immediate remedy (this version has the standard 12 column output). Sequence length data could always be

Re: [galaxy-user] Running Cufflinks on Bacterial RNAseq data

2012-08-01 Thread Peter Cock
On Tue, Jul 31, 2012 at 7:35 PM, Jennifer Jackson j...@bx.psu.edu wrote: Hello Rachel, When datasets are in a grey waiting to run state this indicates that they are in the queue and in line to run. For the majority of cases, including yours, leaving the job alone and allowing it to run is the

Re: [galaxy-user] Help with Summary Statistics

2012-08-03 Thread Peter Cock
On Thu, Aug 2, 2012 at 7:50 PM, D. A. Cowart dac...@psu.edu wrote: Hello, I am attempting to use Galaxy to calculate the mean sequence read length and identify the range of read lengths for my 454 data. The data has already been organized and sorted by species. The format of the data is as

Re: [galaxy-user] Aggregate collections of files (example: Heatmaps)

2012-08-21 Thread Peter Cock
On Tue, Aug 21, 2012 at 4:46 PM, Ted Goldstein t...@soe.ucsc.edu wrote: I am developing several tools that will need to read and write multiple data files at once. For example, Eisen Cluster produces a heatmap which consists of three files: a .cdt file, .atr file and a gtr file which are

Re: [galaxy-user] Installing Galaxy and Hooking into a SGE Cluster

2012-08-23 Thread Peter Cock
On Thu, Aug 23, 2012 at 8:44 PM, mailing list margeem...@gmail.com wrote: Anyway, back to my question. Does anyone know? Would I be better off asking on the developer mailing list or perhaps Stack Overflow? Software licensing is a much broader issue that Galaxy, so yes, perhaps Stack Overflow

Re: [galaxy-user] git mirror

2012-09-04 Thread Peter Cock
On Tue, Sep 4, 2012 at 5:57 PM, Luca Pireddu pire...@crs4.it wrote: Hello list. A simple question: is there a git mirror of the Galaxy repositories? If not, what do git users here do to work with the Galaxy code base? Thanks, I don't think there is an official git mirror, but even if there

Re: [galaxy-user] blast tools on free public server

2012-10-03 Thread Peter Cock
On Wed, Oct 3, 2012 at 3:54 PM, John DeFilippo defilippo.j...@gmail.com wrote: We're using the free public Galaxy server (main.g2.bx. psu.edu). We uploaded a genome FASTA sequence via ftp, and want to do BLAST searches (e.g., tblastn) against protein sequences that we've also uploaded to

Re: [galaxy-user] Cannot install ncbi_blast_plus or other tools from Tool Shed: HTTP 500 error

2012-10-08 Thread Peter Cock
On Mon, Oct 8, 2012 at 8:26 AM, Joel Rosenberg thisisj...@hotmail.com wrote: So I'm trying to install the recently migrated ncbi_blast_plus tool from the Tool Shed to my local galaxy instance running in EC2 and am getting errors at the installation step. After clicking Install to local

Re: [galaxy-user] How to merge Fastq groomer files in Galaxy?

2012-10-17 Thread Peter Cock
On Tue, Oct 16, 2012 at 8:22 PM, Fang,Xiefan xiefanf...@ufl.edu wrote: Dear Galaxy users, Does anyone know how to merge several FASTQ groomer files by using Galaxy? If not, is there any other program that can achieve this? The size of one FASTQ groomer file is around 1GB. Thank you!

Re: [galaxy-user] stderr on Samtools sort

2012-11-14 Thread Peter Cock
On Wed, Nov 14, 2012 at 9:00 AM, Masaki MS msmorioka-...@umin.ac.jp wrote: Dear all, I'm trying to set up samtools sort command on local Galaxy server. But I can not success to get sorted files from its result. I thought Galaxy tried to keep SAM/BAM files sorted by default... First time, I

Re: [galaxy-user] Find out sense and antisense sequences

2012-11-26 Thread Peter Cock
On Mon, Nov 26, 2012 at 6:47 PM, Zhiqiang Shu z...@bio.fsu.edu wrote: Hi, Galaxy users! I have a question on how to find out sense and antisense sequence. I've got RNA seq data in the fastq format. The sequences inside are partially complementary to each other (complementary is 10nt, while

Re: [galaxy-user] combining two fastq files

2012-11-27 Thread Peter Cock
On Tue, Nov 27, 2012 at 9:51 PM, shamsher jagat kanwar...@gmail.com wrote: Is there an option in galaxy to combine two fastq files? Thanks kanwar Yes, but what do you mean by combine? Interleave? Concatenate? Peter ___ The Galaxy User

Re: [galaxy-user] User's information

2012-11-30 Thread Peter Cock
On Friday, November 30, 2012, Sachit Adhikari wrote: Where are the user's information stored in Galaxy? I can't find the information in universe_wsgi. Regards, Sachit It is in the database (PostgreSQL usually, but MySQL is also supported, and for simple test setups even SQLite mostly

Re: [galaxy-user] [galaxy-dev] History not updating for multiple instances of Galaxy

2013-02-21 Thread Peter Cock
On Thursday, February 21, 2013, wrote: Further, when I try “register” I add an email address and password, but the system still doesn’t log me in ** ** Maybe this is a database configuration issue? ** ** I didn’t think I’d need to state any specific entries in universe as I am

Re: [galaxy-user] Change format with edit attributes

2013-04-03 Thread Peter Cock
On Wed, Apr 3, 2013 at 3:40 PM, Gema Sanz Santos ge2sa...@gmail.com wrote: Hello, I'm trying to change the format to the output files from Barcode splitter from FASTA to FASTAQ so I can use them in Bowtie for Illumina. I've read that it can be done through the edit attributes, I go to

Re: [galaxy-user] GenBank database files

2013-04-28 Thread Peter Cock
On Sun, Apr 28, 2013 at 11:22 PM, Mike Dyall-Smith mike.dyallsm...@gmail.com wrote: This should be easy (but not for me so far). I want to do local blast searches, so I download the premade nr protein blast database from GenBank. It is split into 10 .tar.gz files. I've decompressed them

Re: [galaxy-user] GenBank database files

2013-04-29 Thread Peter Cock
On Mon, Apr 29, 2013 at 3:27 AM, Mike Dyall-Smith mike.dyallsm...@gmail.com wrote: Dear Peter Cock, thanks for your advice. Just to be clear, do I leave the files within their decompressed folders or do I put all the individual files into one folder? I assume the former, but want to be sure

Re: [galaxy-user] Filter Fastq

2013-05-09 Thread Peter Cock
On Thu, May 9, 2013 at 12:27 PM, Casey,Richard richard.ca...@colostate.edu wrote: Hi, We have two Filter FASTQ jobs running on the Galaxy public server. Both jobs have been running for more than four days. This seems like an excessive amount of runtime. Do Filter FASTQ jobs normally take

Re: [galaxy-user] Rename tool output file in Galaxy

2013-07-24 Thread Peter Cock
On Wed, Jul 24, 2013 at 5:21 AM, Sachit Adhikari sachit.techner...@gmail.com wrote: Hi group, The galaxy stores the output of the job on files/043/dataset_ID.dat. I have two questions here.: 1) Can I find the ID of my output from the Galaxy history? I tried Edit Attributes, Annotations,

Re: [galaxy-user] Rename tool output file in Galaxy

2013-07-24 Thread Peter Cock
On Wed, Jul 24, 2013 at 10:35 AM, Sachit Adhikari sachit.techner...@gmail.com wrote: Hi, By i do you mean Edit attributes button? There are four sub-headings on that: Attributes, Convert format, Datatype and permissions but I can't see data file's full path on any of them. What's wrong? No,

Re: [galaxy-user] Rename tool output file in Galaxy

2013-07-24 Thread Peter Cock
On Wed, Jul 24, 2013 at 11:15 AM, Sachit Adhikari sachit.techner...@gmail.com wrote: Yes, but unfortunately that didn't work too. I have already tried the solution you provided. I could edit the tags and annotations but it won't change the file name in my file system. The file is still named as

Re: [galaxy-user] viewing data live while tool is running

2013-07-30 Thread Peter Cock
On Tue, Jul 30, 2013 at 5:27 PM, Ketan Maheshwari ketancmaheshw...@gmail.com wrote: Hi, I am a relatively new Galaxy user. I am wondering if there is an example of a tool which allows users to view at the stdout/err of a tool live while it is still running to get a sense of progress it is

Re: [galaxy-user] Bowtie: Outputting unmapped reads

2013-07-31 Thread Peter Cock
On Wednesday, July 31, 2013, Mayank Tandon wrote: That's a neat trick, and I definitely wouldn't have thought of that approach, so thanks for that! After I finished writing this out, I realized it was super long. So here are the questions I'm asking up front, so you can choose whether or

Re: [galaxy-user] tool within a tool

2013-08-22 Thread Peter Cock
On Thu, Aug 22, 2013 at 12:07 AM, Ketan Maheshwari ketancmaheshw...@gmail.com wrote: Hi, I am wondering if it is possible in Galaxy to design a tool whose sole purpose is to run other tools. This is motivated by our desire to enhance execution capabilities of existing tools via a generic

Re: [galaxy-user] watching command line to a query

2013-08-27 Thread Peter Cock
On Tue, Aug 27, 2013 at 2:07 PM, lilach noy lilach...@gmail.com wrote: Thank you for the reply. Where can i find the log file? As an ordinary Galaxy user, I'm not sure if this is possible. You would have to be running your own Galaxy instance, look at the file paster.log in the main Galaxy

Re: [galaxy-user] generating pileup with max depth greater than 8000?

2013-09-26 Thread Peter Cock
On Wed, Sep 25, 2013 at 7:27 PM, Lauren Oldfield lm...@pitt.edu wrote: My question: How can I generate a pileup with an output of more than 8000 hits per base? I was generating pileups using the SAM tools -- Generate pileup and do not see an option to change the settings for output. In mpileup

Re: [galaxy-user] help for trim sequences

2013-11-23 Thread Peter Cock
On Fri, Nov 22, 2013 at 8:48 PM, Jennifer Jackson j...@bx.psu.edu wrote: Hi Seung Hee, I know we discussed this on the other list, but I didn't point you to the open development ticket to (potentially) extend the functions of the Cut tool. This is not being actively worked on right now, but

Re: [galaxy-user] fastqc and blast? trinity?

2013-12-14 Thread Peter Cock
On Sat, Dec 14, 2013 at 8:52 AM, Jorge Braun braun_...@hotmail.com wrote: Hello, of course, Jennifer is right for the first question . For the second question about blast ... I wonder if running after blast in galaxy I can remove sequences that can contaminate the data. It's possible? The

Re: [galaxy-user] usegalaxy.org is down?

2014-01-20 Thread Peter Cock
On Mon, Jan 20, 2014 at 3:53 PM, Leontovich, Alexey A., Ph.D. leontovich.ale...@mayo.edu wrote: Hello, It seems that usegalaxy.org is down. Is it? Thank you. Alexey Leontovich It was down yes, but should be OK now: @GalaxyProject on Twitter recently: After a short downtime we are back up

Re: [galaxy-user] special character $ gets converted to X in tool

2014-01-31 Thread Peter Cock
On Fri, Jan 31, 2014 at 5:13 PM, Ketan Maheshwari ketancmaheshw...@gmail.com wrote: Hi, In a test tool that I am working on, I need to enter text preceded by a $ sign to be interpreted as an environment variable by the underlying running script. However, it seems that the $ sign gets

Re: [galaxy-user] Galaxy + user program unable to locate files

2014-02-27 Thread Peter Cock
On Thu, Feb 27, 2014 at 9:32 AM, do kadya doka...@gmail.com wrote: Hi, Thank You for your kind reply. I am having problem in running command in Galaxy. my current command: tool_name file_having_multiple_file_name_tab_delimited.txt or .tab file1.bam file2.bam /usr/bin/x_tool

Re: [galaxy-user] Galaxy + user program unable to locate files

2014-02-27 Thread Peter Cock
On Thu, Feb 27, 2014 at 4:20 PM, do kadya doka...@gmail.com wrote: I don't think so that problem is in tool, because when I run same tool of normal terminal, it generate output. This is why I was asking about Perl environment variables - they may be different compared to when you run the tool.

Re: [galaxy-user] Unsubscribe

2014-03-14 Thread Peter Cock
On Fri, Mar 14, 2014 at 9:58 AM, Elvio Pederzolli epede...@bigpond.net.au wrote: Hi there can you please unsubscribe me from all Galaxy lists please Thank you Elvio Pederzolli You should be able to unsubscribe yourself here: http://lists.bx.psu.edu/listinfo/galaxy-user Regards, Peter

[galaxy-user] BioStar integration - merging accounts

2014-04-24 Thread Peter Cock
Hi all, I see that https://biostar.usegalaxy.org is live now, with lots of content mined from the galaxy-user mailing list (which may or may not result in this email starting a new question? We shall see...). Is it possible for an admin to merge accounts? e.g. there are all me:

Re: [galaxy-user] BioStar integration - merging accounts

2014-04-24 Thread Peter Cock
On Thu, Apr 24, 2014 at 2:40 PM, Peter Cock p.j.a.c...@googlemail.com wrote: Hi all, I see that https://biostar.usegalaxy.org is live now, with lots of content mined from the galaxy-user mailing list (which may or may not result in this email starting a new question? We shall see

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Peter Cock
Hi Scott, Could you clarify *which* Galaxy server you are using, and *which* megablast tool within Galaxy? Most BLAST options within Galaxy do NOT send the queries to the NCBI servers. (1) Main Public Galaxy I would guess you are using the main Galaxy Server, which only has this megablast

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Peter Cock
On Mon, Apr 28, 2014 at 9:01 PM, Scott Tighe scott.ti...@uvm.edu wrote: Peter Thank you for your detailed response and I should have noted that I was using the Main Public Galaxy. Thank you for confirming that it is down! I appreciate your input! Scott Hi Scott, Galaxy is back for me