Re: [ccp4bb] Improve diffraction ...any ideas?

, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Improve diffraction ...any ideas?

. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Improve diffraction ...any ideas?

On May 24, 2013, at 8:01 AM, Patrick Shaw Stewart patr...@douglas.co.uk wrote: Use random microseeding to pick up new conditions, and work with those. See http://www.douglas.co.uk/mms.htm and http://www.douglas.co.uk/MMS_proc.htm for theory, references and practical

Re: [ccp4bb] Protein concentration for crystallization

the sender by reply e-mail message and destroy all copies of the original message (including attachments). -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http

Re: [ccp4bb] structural homologs as cross seeds

, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] seeding, reproducibility

suggestions Mahesh -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg

Re: [ccp4bb] seeding, reproducibility

that it was so big. Have a nice weekend ! Mahesh On Fri, Jan 17, 2014 at 6:18 PM, Patrick Shaw Stewart patr...@douglas.co.uk wrote: Mahesh, this is a very interesting and slightly controversial question. One approach is to mix together all of the crystals that you have in the initial screen

Re: [ccp4bb] First stucture of FCFV

...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] PDB passes 100,000 structure milestone

. -- Ethan A Merritt Biomolecular Structure Center, K-428 Health Sciences Bldg MS 357742, University of Washington, Seattle 98195-7742 -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw

Re: [ccp4bb] PDB passes 100,000 structure milestone

concluded that it was, more likely than not, faked [1] [1] http://www.nature.com/news/2009/091222/full/462970a.html On 15 May 2014 18:00, Nat Echols nathaniel.ech...@gmail.com wrote: On Thu, May 15, 2014 at 9:53 AM, Patrick Shaw Stewart patr...@douglas.co.uk wrote: It seems to me

Re: [ccp4bb] PDB passes 100,000 structure milestone

the necessary wikitext references/). See *http://proteopedia.org/w/Help:Editing#Citing_Literature_References http://proteopedia.org/w/Help:Editing#Citing_Literature_References* and Proteopedia pages for actual examples. best regards, Jaime Joel On 15May, 2014, at 13:48, Patrick Shaw Stewart patr

Re: [ccp4bb] Off-topic: Supplying PDB file to reviewers

...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] zinc with HEPES/seeding

this Zn in not compatible with HEPES. Could you please tell me why is this? I appreciate your help. Thanks Rajesh -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw

Re: [ccp4bb] Crystallization with antibody

...@douglas.co.uk    Douglas Instruments Ltd.  Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk  Tel: 44 (0) 148-864-9090    US toll-free 1-877-225-2034  Regd. England 2177994, VAT Reg. GB 480 7371 36

[ccp4bb] Punctuation etc.

help people to understand what you're asking. Giving your message a meaningful title is also very helpful! Best wishes to all, Patrick -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw

Re: [ccp4bb] Improvement in crystal quality

Laboratory,Pune,India -- The most difficult phase of life is not when No one understands you;It is when you don't understand yourself -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart

Re: [ccp4bb] Improving microcrystals

Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Off-topic: Best Scripting Language

programming language is beautiful. James -- James Stroud http://www.jamesstroud.com -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0

Re: [ccp4bb] off topic: reproducing hits from organic solvents

, Peter -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB

[ccp4bb] Plate crystals

than routine optimization method in the lab? Thanks in advance. Jahan -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090

Re: [ccp4bb] Stabilization of crystals and ligand exchange

Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

[ccp4bb] inflluence of pH for crystallization on protein 3-D structure

Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -- patr...@douglas.co.ukDouglas

Re: [ccp4bb] Problems in crystallization

, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Glass Capillaries

Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Equilibrium Relative Humidity matching

(NH4+ and (NH4SO4)-) and not 3 as might be expected). This means that relatvie humidity is a colligative property: http://en.wikipedia.org/wiki/Colligative_properties Doesn't it? So it should not be too surprising. Boaz On 22/11/2012 18:32, Patrick Shaw Stewart wrote: Matt

Re: [ccp4bb] To cryo or not to cryo...

. best, -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg

Re: [ccp4bb] protein crystals or salt crystals

, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] protein crystals or salt crystals

1mM. best regards Amr -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd

Re: [ccp4bb] salt or not?

. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] crystal friendly solvents that are useful for dissolving hydrophobic small molecules?

) microbatch-under-oil. The oil acts as a reservoir that contains excess small molecule that (you hope) will be fed into the crystals. Patrick Shaw Stewart -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock

Re: [ccp4bb] Question about glove boxes for protein crystallization

Hi Mathews One of our customers, Bret Dillard at the University of Georgia, has had great success with one of our robots in a Bactron X anaerobic chamber. In this case, Bret mainly used the microbatch-under-oil method, which works very well for anaerobic work: (1) the oil protects the sample

Re: [ccp4bb] Main topic of the day: Protein crystallization

Ronaldo I have a database of crystallization conditions extracted from the PDB. I was able to parse the data to extract the concentration of protein in around 900 cases. The lowest 9 protein concentrations were as follows: PDB ID TYPEDATEProtein conc, mg/ml 1EYMISOMERASE.

[ccp4bb] Demonstration and support specialist

. No salesmen please. Please reply to the address below, or e-mail. Patrick Shaw Stewart --  [EMAIL PROTECTED] Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://douglas.co.uk or http

Re: [ccp4bb] Help with reducing crystal mosaicity

25.7%. You can see the data at www.douglas.co.uk/top14.htm I thought this information could be useful if you want to replace some of the MPD with another precipitant (or cryoprotectant). Best wishes Patrick Shaw Stewart --  [EMAIL PROTECTED] Douglas Instruments Ltd.  DouglasHouse

[ccp4bb] Fwd: [ccp4bb] crystallisation robot

One thing that people often overlook is that quite a lot of protein can be lost by denaturation on the surface of the drop. This is more significant for smaller drops. Two suggestions: (1) increase the proportion of protein in the - technical term - teeny drop to say two thirds and (2) cover the

Re: [ccp4bb] isopropanol as a precipitant

know if anyone wants samples. Patrick -- [EMAIL PROTECTED]Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart, James Smith http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1

Re: [ccp4bb] about microbatch

, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jiamu Du Sent: 26

Re: [ccp4bb] Query on program for creating random crystallization screen

, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

, please join our bulletin board at http://groups.google.com/group/oryx_group/members_invite?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148

Re: [ccp4bb] microbatch vs hanging drop

Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board

Re: [ccp4bb] Scaling up from an Intelliplate to Linbro Plate

://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034

Re: [ccp4bb] turn granular to crystal

, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of rui Sent: 25 August

[ccp4bb] Fab purification and crystallization

Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp

Re: [ccp4bb] crystallizing a complex that's sensitive to ionic strength

, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Reproducing crystals.

? Please help me. Thanks in advance. -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England

Re: [ccp4bb] small lysozyme crystals?

this before? -James Holton MAD Scientist --  patr...@douglas.co.uk    Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk  Tel: 44 (0) 148-864-9090    US toll-free 1-877-225-2034  Regd

Re: [ccp4bb] Fab:antigen complex crystallization!!!

. Thanks ivan -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg

Re: [ccp4bb] Fab:antigen complex crystallization!!!

to make water is crazy?     Julian, King of Lemurs --  patr...@douglas.co.uk    Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk  Tel: 44 (0

Re: [ccp4bb] Crystals with Organic solvents

.  Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk  Tel: 44 (0) 148-864-9090    US toll-free 1-877-225-2034  Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] UV imaging of crystals

, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Complex seeding

Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] neutron data measurement

University of Toledo -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT

Re: [ccp4bb] Protein melting temperatures

, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Protein melting temperatures

at is if your transition is sharp (the steeper the better). Taking all this together you can judge if your protein is happy or not. Hope this helps you! Linda Patrick Shaw Stewart wrote: I actually think you *can *make comparisons between different proteins. We heard a very nice talk

Re: [ccp4bb] IUCr committees, depositing images

-- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480

Re: [ccp4bb] Disappearing crystals

, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] No diffraction

with mass spec. Any suggestions to improve diffraction would be welcome. Thanking you in advance. Theresa -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http

Re: [ccp4bb] Dye for protein affinity measurement

,thank kind-hearted people in advance! Regards Jiahong -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US

Re: [ccp4bb] Membrane Protein Crystallization Plates

-- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Desalting columns

Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] microseeding

if you could advise and share some practical ways to further my experiment. Thanks in advance Regards, Rajesh -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http

Re: [ccp4bb] Unable to reproduce robot tray hits in hand trays

screening. There's a page on our web site which explains this in much more detail, see http://www.douglas.co.uk/Scaling_Up.htm Best wishes Patrick -- Forwarded message -- From: Patrick Shaw Stewart patr...@douglas.co.uk Date: 19 March 2012 22:29 Subject: Re: [ccp4bb] microseeding

Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

! There's more info at http://www.douglas.co.uk/rat_des.htm Patrick --  patr...@douglas.co.uk    Douglas Instruments Ltd.  DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK  Directors: Peter Baldock, Patrick Shaw Stewart  http://www.douglas.co.uk/  Tel: 44 (0) 148-864-9090    US toll

Re: [ccp4bb] How to improve crystal which is twinning?

, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board

Re: [ccp4bb] anaerobic crystallization

patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] stabilizing solution

?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480

[ccp4bb] Vapor diffusion calculator

Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jacob Keller Sent

Re: [ccp4bb] 24-well sitting-drop plates

protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http

Re: [ccp4bb] freezing crystals grown in isopropanol condition

to deal with isopropanol evaporation during mounting? Many thanks and best wishes, Chris -- patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148

Re: [ccp4bb] control of nucleation

Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of zq deng Sent: 06 May 2010 09:04 To: CCP4BB@JISCMAIL.AC.UK

Re: [ccp4bb] frozen pellet insoluble protein

for Structural Biology Imperial College London -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1

Re: [ccp4bb] fastening crystal formation

Addlagatta, Senior Scientist, CSIR-IICT, Tarnaka, Hyderabad, India-57 Mobile: +918886922975 -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Off-topic - Crystallisation in anaerobic glove box

://www.mrc.ac.uk/About/informationandstandards/documentmarking/index.htm . -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090

Re: [ccp4bb] Off-topic - Crystallisation in anaerobic glove box

, but I would think they also dissolve O2 readily. eab On 03/18/2015 08:02 AM, Patrick Shaw Stewart wrote: Hi Steve I have one more comment for this thread. The microbatch-under-oil method is very handy for anaerobic work: 1. You can keep the microbatch stock solutions

Re: [ccp4bb] Choice of stereomicroscope

. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] how to reduce protein solubility

Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] handling crystals in volatile solvents

7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Crystals grow at the bottom of the tube.

om > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Slightly OT: crystallization teaching resources for kids

; Mayo Clinic Cancer Center > > Griffin Cancer Research Building > > 4500 San Pablo Road > > Jacksonville, FL 32224 > > tel: 904-953-6372 > > fax: 904-953-0277 > > > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] crystallization optimization

7 06:28, Vicky Tsirkone wrote: >> >> Dear Patrick, >> >> You may reduce the protein concentation, as well. >> Another option could be the *streak seeding* by exploiting the drop of >> your initial condition. >> >> Good luck, >> >> V.T. &g

Re: [ccp4bb] crystallization optimization

acie.univ-paris5.fr%2F> > , http://lcrbw.pharmacie.univ-paris5.fr/spip.php?article18 > <https://clicktime.symantec.com/a/1/SiKnIzlCgLoTAR5pxIlBBAWAsBmNDNdJ41f4ue88mvg=?d=ydHuK2RzVboI0S1f0-VMFO47v0jLw-bGOuPIOXlvgxVovpKjpVbqlqHHe92eD9EXP1yEiYalPcDJ38NT0DPj0JHU1ay-7q9vqP6cG6Jh_Y2NFwbuGut6uA1GqbEf11-ROhtHIifuMGSuiUO4Yxf9ZKoKqiIJZmSrtwlOii1OALADi7UtKkI_mrwwLYA4QugHgmtOUOK5Mw1HMEYTM0eLrh1UB5KlzbR1gdvNDPZRm1oDPNOSmHAOma5Mdveve6ZlC5WH6mgCC5qcLQd_xWchIlNmFOVWl0_LVlkIZxPEbwq

Re: [ccp4bb] crystallization optimization

ectors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Granada Crystallization Boxes?

glas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Protein or DNA crystals

las Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] crystallization screen for protein-protein complex

to try? > > Looking for your help... > Regards, > Hena. -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US to

Re: [ccp4bb] Regarding Patents

hts is to publish it so the world can > benefit from it. > > Gert > > > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Na-Binding Protein?

; 765-494-1607 > -- > *Research Lab Contact Information:* > Hockmeyer Hall of Structural Biology > Room 311 > <https://maps.google.com/?q=311+240+S.+Martin+Jischke+Drive+West+Lafayette,+IN+47907=gmail=g> > 240

Re: [ccp4bb] crystals that dont diffract :( :(

> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK D

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

from mobile Patrick Shaw Stewart +44 7901 548 201 On Wed, 15 Aug 2018, 08:41 Yvonne Thielmann, wrote: > Dear Thomas, > > maybe you can try to overlay your drop with LV CryoOil from Jena > Bioscience. Then the evaporation of the solvent is slowed down and the > crystals are d

Re: [ccp4bb] Oxford University Press

rof. George M. Sheldrick FRS > Dept. Structural Chemistry, > University of Goettingen, > Tammannstr. 4, > D37077 Goettingen, Germany > Tel. +49-551-39-33021 or +49-5594-227312 > > > -- > > To unsubscribe from the CCP4BB list, click the following

Re: [ccp4bb] Please share your experience about "ugly" crystals showing good diffraction

he following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > -- patr...@douglas.co.ukDouglas Ins

Re: [ccp4bb] suggestions on a microscope for Crystallography

olarization, light source (fiber optics), crosshairs and > camera mount. What Models and make? > > Thanks in advance > > Chandra > > > > > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Direc

Re: [ccp4bb] Anderson?Evans polyoxotungstate (TEW)

8 > 69120 Heidelberg > Germany > Phone: +49 6221 54 4796 > Email: <nikolay.dob...@bzh.uni-heidelberg.de > > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

c.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > > > > > > > > > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > > > > > > #### > &

Re: [ccp4bb] Optimization from needle shaped crystals

gt; To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Sha

Re: [ccp4bb] Intergrown crystals and excess of nucleation

ttps://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-fr

Re: [ccp4bb] Importance of temperature during initial crystallization screening

e following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel:

Re: [ccp4bb] challenges in structural biology

I take the view that I'm trying to communicate with as many people as possible, without distracting them with my spelling . . . So go for US spellings. Sent from mobile On Tue, 23 Jul 2019, 22:39 Goldman, Adrian, wrote: > ..and responding in the same vein: > > my OED says that its etymology

Re: [ccp4bb] challenges in structural biology

gt; > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Gars

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