Re: [galaxy-dev] cufflinks libz error/warning

2011-03-17 Thread Jeremy Goecks
Hi all - I'm running Galaxy on my Redhat linux cluster. When I run cufflinks from the shell I get the error/warning: cufflinks: /usr/lib64/libz.so.1: no version information available (required by cufflinks) but it still seems to run ok. It looks like Galaxy is choking on this. I tried

Re: [galaxy-dev] Missing Page Editor Components

2011-03-17 Thread Jeremy Goecks
However, when I tried to use the Edit Pages function, there is no Embed Galaxy Object and Insert Link to Galaxy Object in the Page Editor. I have set the enable_pages = True in universe_wsgi.ini. Wonder what else can cause the problem? Charlie, This bug has been fixed in galaxy-central

Re: [galaxy-dev] cufflinks libz error/warning

2011-03-18 Thread Jeremy Goecks
It looks like the output only occurs when cufflinks can't run on the provided dataset. I checked my inputs, and I think that is the problem...its a sam file from bowtie, not from tophat, hence not a sorted sam file. Perhaps this stderr message doesn't matter after all, its just

Re: [galaxy-dev] Error when trying to download workflow

2011-04-07 Thread Jeremy Goecks
Hi Sarah, I created your workflow on my Galaxy instance and was able to export it successfully, so updating your Galaxy instance may solve your problem. If not, let us know and we'll take a closer look. Finally, please direct your questions to one of our mailing lists rather than individual

Re: [galaxy-dev] Question about cuffcompare tool implementation

2011-04-11 Thread Jeremy Goecks
Assaf, Assuming cuffcompare generates a TMAP and REFMAP file for each input file, does this mean the wrapper tool retrieves only the first and second input files' TMAP and REFMAP files ? Yes, this is the way it works currently. This is clearly problematic, but it meets most users' needs

Re: [galaxy-dev] Error when trying to download workflow

2011-04-14 Thread Jeremy Goecks
workflow whose download fails. I recreated the workflow at the public instance at http://main.g2.bx.psu.edu and shared it with you. There the download also fails for me. Best regards, Sarah On 04/14/2011 02:55 PM, Jeremy Goecks wrote: Sarah, Can you make your server available to me

Re: [galaxy-dev] Email notification and Workflow sharing for all users

2011-04-18 Thread Jeremy Goecks
Paul, Sorry for the slow reply. We've been discussing this issue internally: For the project that I'm currently working on, I need to have a link to one particular workflow appear in the Tools left-hand side panel/frame for every user that logs in to Galaxy. They shouldn't have to import

Re: [galaxy-dev] Update problem

2011-04-18 Thread Jeremy Goecks
Olen, This is a runtime error, not an update error; it is occurring because we mistakenly committed code that is not compliant with python 2.4 Here are your options to fix this error: (1) update your python version from 2.4 to 2.5 or 2.6; (2) update your installation from galaxy-central;

Re: [galaxy-dev] question about Filtering Cufflink files

2011-05-03 Thread Jeremy Goecks
Hello, This is a bug and has been fixed. I reran your analyses on our test server and they completed successfully, so you should be good to go. Best, J. On May 3, 2011, at 6:08 AM, shamsher jagat wrote: Jeremy, I have been trying to follow the steps in filtering Cufflink out put files

Re: [galaxy-dev] Update of cufflinks

2011-05-10 Thread Jeremy Goecks
Sarah, We recently updated the Cufflinks/compare/diff wrappers to be compatible with v1.0.* ; the wrappers are available in galaxy-central and should be available in galaxy-dist in the next couple weeks as we're planning another release soon. New features in v1.0.* have not been implemented in

Re: [galaxy-dev] Update of cufflinks

2011-06-06 Thread Jeremy Goecks
. -gordon Jeremy Goecks wrote, On 05/10/2011 09:02 AM: We recently updated the Cufflinks/compare/diff wrappers to be compatible with v1.0.* ; the wrappers are available in galaxy-central and should be available in galaxy-dist in the next couple weeks as we're planning another release soon

Re: [galaxy-dev] question/bug with cuffcompare wrapper

2011-06-07 Thread Jeremy Goecks
Assaf, Thanks for the very helpful information. We largely sync our tool wrappers to the tool version used on our public server. We're currently running v1.0.1 of Cufflinks on main; hence, as you discovered, the wrappers are compatible with that version of Cufflinks but (sadly) incompatible

Re: [galaxy-dev] CuffDiff output mis-named ?

2011-06-07 Thread Jeremy Goecks
Based on the naming convention of the other outputs (and the output files from cuffdiff), I think the label of the first one should be renamed to CDS Expression only (assuming the relevant input file cds_exp.diff). Correct. That said, I've been planning to reorder and rename the Cuffdiff

[galaxy-dev] Fwd: [galaxy-user] Galaxy tool error report from joh...@ibms.sinica.edu.tw

2011-06-09 Thread Jeremy Goecks
John, Questions about local installs are best directed to galaxy-dev, so I've moved the question to this list. While executing cuffcompare, I encountered this error message: Error running cuffcompare. [Errno 2] No such file or directory: 'cc_output' I was practicing RNA-seq analysis

[galaxy-dev] Fwd: a Trackster Question

2011-06-16 Thread Jeremy Goecks
Matt, I'm moving your Trackster question onto the galaxy-dev list: one issue I keep running into is that it's not super clear how to go about configuring the dynamic tracks you showed off in your talk. Is there some guidance somewhere that I am missing on this? I confess my own motive

Re: [galaxy-dev] BAM display at Trackster

2011-07-07 Thread Jeremy Goecks
Yes, all BAM fails are failing with the same error message. Before updating my instance I did not try to visualize through Trackster. Samtools path are available to Galaxy. I am not seeing any error/warning message in my log file. Try this in the main interface: (1) click the pencil

Re: [galaxy-dev] Multiple fastq files per forward/reverse mates (tophat and related tools)

2011-07-11 Thread Jeremy Goecks
I recommend to use the repeat tag (see http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Config%20Syntax) around the existing parameter to make it possible to select multiple files. Sarah's suggestion is spot on. See the Cuffcompare wrapper for an example of how to use this tag. Gus, if you end

Re: [galaxy-dev] Multiple fastq files per forward/reverse mates (tophat and related tools)

2011-07-15 Thread Jeremy Goecks
, 2011 at 8:51 AM, Jeremy Goecks jeremy.goe...@emory.edu wrote: I recommend to use the repeat tag (see http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Config%20Syntax) around the existing parameter to make it possible to select multiple files. Sarah's suggestion is spot on. See the Cuffcompare

Re: [galaxy-dev] Newer Version of Cufflinks Wrapper

2011-07-20 Thread Jeremy Goecks
Ilya, Despite the wrapper version numbers (which I'll update shortly to avoid further confusion), the current Cufflinks wrapper in both galaxy-central and galaxy-dist supports Cufflinks v1.0.3 However, not all new options for v1.0.3 are implemented the current wrappers; should you decide to

Re: [galaxy-dev] Extracting regions of sequences given table of co-ords

2011-08-11 Thread Jeremy Goecks
It turns out there sort of is, extract/extract_genomic_dna.xml However, as written it isn't clear to me how to make this work with general tabular files, and how it would behave with proteins (which in principle can be process the same way). It also appears to insist on having both

Re: [galaxy-dev] Updated Cufflinks Wrapper

2011-08-11 Thread Jeremy Goecks
Hi Ilya, Thanks for the contribution; most of it has been committed in changeset 0ef81af1de09 A couple notes: *I couldn't transplant your changes because your commit did not include recent changes to the Cufflinks wrapper. It appears you didn't merge correctly with the main repository at

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-12 Thread Jeremy Goecks
Actual result: Red error against the gff file, Unspecified genome build, click the pencil icon in the history item to set the genome build The fact I'm using a FASTA file from my history should mean the genome build is irrelevant as that only applies to locally cached genomes (right?).

Re: [galaxy-dev] trackster reference genome

2011-08-14 Thread Jeremy Goecks
Colin, How is it possible to preinstall a reference genome for trackster (hg19) within galaxy. i tried to find a dedicated help page but could not find any. Could you please link me to relevant information? See the section titled 'Setup for local instances' on this page:

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-14 Thread Jeremy Goecks
Great. Could you try that example on the latest galaxy-central please? I have revision 06f0bca6de24 and get the following error using the steps given earlier: Traceback (most recent call last): File /home/pjcock/repositories/galaxy-central/tools/extract/extract_genomic_dna.py, line

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-14 Thread Jeremy Goecks
I think I've found another problem in exploring possible workarounds, 1. Goto http://usegalaxy.org (or a local Galaxy running galaxy-dist or galaxy-central) 2. Import this GFF3 file, ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Nanoarchaeum_equitans_Kin4_M_uid58009/NC_005213.gff 3. Click on

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-14 Thread Jeremy Goecks
Well, sort of. After converting that GFF3 file into BED, the strand column isn't set in the metadata. That seems important! We'll look into this. Also a point of clarification, I had the wrong URL for the FASTA file. This is the whole genome, although to actually proceed with this

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-15 Thread Jeremy Goecks
However, the FASTA output uses different names because it embeds the start/end co-ordindates as is. Thus using GFF3 features, the sequence name includes _883_2691_ while using BED features the same sequence has instead _882_2691_ for its name. I propose this be harmonised by always using

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-15 Thread Jeremy Goecks
Sorry -more questions - could you explain what the Interpret features when possible setting in the Extract Genomic DNA is meant to do? The tool's help text doesn't say anything (other than it is only for GTF/GFF files). In the NC_005213.1 example turning Interpret features when possible on

Re: [galaxy-dev] Extract Genomic DNA insisting on build for GFF3 file

2011-08-16 Thread Jeremy Goecks
One idea to address both of these issues is to embed the original format in the fasta name so that it's clear whether the coords are BED or GFF (e.g. hg17_BED_chr1_147962192_147962580). Or hg17_gtf_chr1_147962192_147962580 etc. That certainly seems better than the current situation.

Re: [galaxy-dev] trackster problem with IE8 and IE7 when using June 23, 2011 release

2011-08-19 Thread Jeremy Goecks
Hi Hans, We haven't done much testing of Trackster with IE yet, and I wouldn't be surprised if it's broken because IE doesn't follow standards in many important areas. Trackster should work with Chrome, Safari, and Firefox in all cases. We'll continue to work to improve Trackster functionality

Re: [galaxy-dev] filtering BED files by score in trackster

2011-08-25 Thread Jeremy Goecks
The option to filter a BED file by the score in the visualization tool is brilliant! There is just one draw back: you can only filter by integers. Hence a score of 1.1 is the same as of score of 1.3. How difficult would it be to change the code in order to allow floats for the filter?

Re: [galaxy-dev] cuffcompare wrapper

2011-09-08 Thread Jeremy Goecks
a problem as far as I have tested. We don’t want to have our code base differ to much from galaxy-central. Thanks, Ilya From: Jeremy Goecks [mailto:jeremy.goe...@emory.edu] Sent: Wednesday, September 07, 2011 6:26 PM To: Chorny, Ilya Cc: galaxy-dev@lists.bx.psu.edu Subject: Re

Re: [galaxy-dev] Loading Histories etc

2011-09-22 Thread Jeremy Goecks
Hi Shaun, I have built up a large collection of Galaxy data on my local account and I am now finding the following operations very slow: Showing saved histories I just committed a fix in galaxy-central that should help out considerably:

Re: [galaxy-dev] Data transfer between two galaxy instances

2011-09-28 Thread Jeremy Goecks
Len, This a beta feature with multiple usability issues. That said, it should work; here's how: Galaxy provides a URL when you export a history; take note of this URL as this is the one that you'll when importing the link. If Galaxy doesn't provide a link, update the history somehow (e.g.

Re: [galaxy-dev] Bug in cufflinks_wrapper.xml?

2011-10-05 Thread Jeremy Goecks
Curt, You are correct, this is a bug. It was fixed in this changeset: https://bitbucket.org/galaxy/galaxy-central/changeset/ff7c8c0bef60 We'll be updating galaxy-dist in the next week and this change will be available then. Thanks, J. On Oct 5, 2011, at 5:54 PM, Curt Palm wrote: I have

[galaxy-dev] hardware for local galaxy install

2011-10-12 Thread Jeremy Goecks
David, Please direct questions about local installations to the galaxy-dev mailing list (cc'd); there are a large number of experienced people of this list that can likely help with this and similar questions. Just a quick question about the compute infrastructure of the setup running the

Re: [galaxy-dev] Some changes to tophat and cufflinks wrappers for indexing loc files

2011-10-12 Thread Jeremy Goecks
I made some changes to the cufflinks and tophat wrapper to pull gtf files from an indexed loc file as opposed to from history. The diff’s are attached. I made it an optional feature. Please let me know if this makes its way into galaxy-central. Ilya, This looks like a good start. A

Re: [galaxy-dev] Version String

2011-10-13 Thread Jeremy Goecks
Shaun, version_command is what you want: version_commandtophat --version/version_command A couple things to note: *tool path isn't needed if the tool is in your galaxy user's path; *your tool must have a --version command line option; if not, you'll have to modify the

Re: [galaxy-dev] Importing Historys in other Galaxy instances

2011-10-26 Thread Jeremy Goecks
Hi Ross, I checked the following: wget link works, I can download the file. It's not saved as Historyname.tar.gz but as export_archive?id=a69ee3e00cb4d02c. Anyway, I can unpack the archive with tar xfvz. hg head: Änderung:5955:949e4f5fa03a Marke: tip Nutzer:

Re: [galaxy-dev] Importing Historys in other Galaxy instances

2011-10-26 Thread Jeremy Goecks
-- select id, state, command_line, stdout, stderr from job where tool_id='__EXPORT_HISTORY__' order by id desc; -- The query should actually be: select id, state, command_line, stdout, stderr from job where tool_id='__IMPORT_HISTORY__' order by id desc; Thanks, J.

Re: [galaxy-dev] cufflinks version

2011-11-02 Thread Jeremy Goecks
David, Wrapper supports v1.0.0 to v1.1.0 I'll update the wiki momentarily. J. On Nov 2, 2011, at 2:04 PM, David Hoover wrote: What version of cufflinks do the current Galaxy wrappers support? The wiki (http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Dependencies) says 1.0.1, but our local

Re: [galaxy-dev] Local galaxy Cufflinks problem

2011-11-16 Thread Jeremy Goecks
Chris, The problem is likely a bug in the Cufflinks wrapper that was recently fixed in galaxy-central: https://bitbucket.org/galaxy/galaxy-central/changeset/ff7c8c0bef60 Try updating your Galaxy instance to include this changeset; if this doesn't fix the problem, let us know. Good luck, J.

Re: [galaxy-dev] Broken published history in a local installation

2011-11-17 Thread Jeremy Goecks
Nico, Does this happen when publishing new histories or just for histories that have been published previously? If you're pulling from galaxy-central, you're running a very old version of Galaxy. Currently tip is 6292:9b7d5c1c0be6 ; updating your instance to tip may solve the problem as well.

Re: [galaxy-dev] Broken published history in a local installation

2011-11-17 Thread Jeremy Goecks
- Postfach 10.2209 69102 Heidelberg, Germany --- On 17 Nov 2011, at 14:59, Jeremy Goecks wrote: Nico, Does this happen when publishing new histories or just for histories that have been published previously

Re: [galaxy-dev] Question about from_work_dir

2011-11-28 Thread Jeremy Goecks
Aurelien, The particular behavior that you need isn't currently supported because the value of from_work_dir isn't treated as a template. To work around this limitation, you can do the move/copy operations in your tool wrapper; see the wrappers for Bowtie and Sicer for examples on how to do

Re: [galaxy-dev] Can't view data files from published histories, only imported histories

2011-11-28 Thread Jeremy Goecks
pas présenter, copier, distribuer ou utiliser le contenu de ce message. Si ce courriel est reçu par erreur, veuillez nous en excuser, veuillez détruire toutes copies de votre système nous informer à supp...@dnalandmarks.ca. From: Jeremy Goecks jeremy.goe...@emory.edu To: Liisa Koski

Re: [galaxy-dev] Question about from_work_dir

2011-11-29 Thread Jeremy Goecks
Aurelien, The particular behavior that you need isn't currently supported because the value of from_work_dir isn't treated as a template. To work around this limitation, you can do the move/copy operations in your tool wrapper; see the wrappers for Bowtie and Sicer for examples on how to

Re: [galaxy-dev] galaxy core services vs wrapper.py duplication caching of .fai files

2011-11-29 Thread Jeremy Goecks
Curtis, [curtish@cheaha galaxy]$ find . -name *.py | xargs grep sam_fa_indices.loc ./tools/samtools/sam_pileup.py:seqFile = '%s/sam_fa_indices.loc' % GALAXY_DATA_INDEX_DIR ... ./tools/ngs_rna/cufflinks_wrapper_without_gtf.py: cached_seqs_pointer_file = os.path.join(

Re: [galaxy-dev] extract_genomic_dna.py checks alignseq.loc?

2011-11-30 Thread Jeremy Goecks
Hi Holger, I went to the respective file and found that the line which defines seq_file is commented out: (l.38 in def check_seq_file()) ## seq_file = %s/alignseq.loc % GALAXY_DATA_INDEX_DIR This seems to be a bug in the current version of the file. There is clearly something amiss. In

Re: [galaxy-dev] Can't view data files from published histories, only imported histories

2011-11-30 Thread Jeremy Goecks
. From: Jeremy Goecks jeremy.goe...@emory.edu To: Liisa Koski liisa.ko...@dnalandmarks.ca Cc: galaxy-dev@lists.bx.psu.edu Date: 2011-11-28 14:46 Subject: Re: [galaxy-dev] Can't view data files from published histories, only imported histories Liisa, I'm not able to reproduce

Re: [galaxy-dev] [galaxy-bugs] Problem with indexing a genome through tophat on a local copy of Galaxy...

2011-12-05 Thread Jeremy Goecks
Hi David, There should be additional information in the galaxy database about why the job failed; take a look at stderr column of the failed job using some SQL like this: -- select * from job where state='error' and tool_id='tophat' and stderr like '%indexing reference%' order by id desc; --

Re: [galaxy-dev] [galaxy-bugs] Problem with indexing a genome through tophat on a local copy of Galaxy...

2011-12-06 Thread Jeremy Goecks
Lecturer in Virology Room E49 Department of Cellular and Molecular Medicine, School of Medical Sciences University Walk, University of Bristol Bristol. BS8 1TD U.K. Tel. +44 117 3312058 Fax. +44 117 3312091 d.a.matth...@bristol.ac.uk On 5 Dec 2011, at 21:00, Jeremy Goecks

Re: [galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-10 Thread Jeremy Goecks
David, What is the full stack trace that you're seeing? The stack trace is the text below Traceback and identifies the exact location where the problem occurs. Are you following these guidelines: http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup for setting up large genomes in Galaxy?

Re: [galaxy-dev] How and where to install tool dependencies

2011-12-10 Thread Jeremy Goecks
Josh, It appears that there are shell directories for the tools under ~/galaxy-dist/tools/ with basic wrapper scripts but without the corresponding executables (very few that I've noticed have the tools already in them). Is the intent to download the dependency tools and (building from

Re: [galaxy-dev] Hidden history item

2011-12-19 Thread Jeremy Goecks
Alex, I just implemented this functionality in galaxy-central changset 303741fda0ec and documented it on the wiki. https://bitbucket.org/galaxy/galaxy-central/changeset/303741fda0ec http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Config%20Syntax#A.3Coutputs.3E_tag_set Best, J. Hi all, by

Re: [galaxy-dev] embedded code into Pages

2011-12-20 Thread Jeremy Goecks
I would like to modify Pages a lot. For example, I would like to create a galaxy Page with rss-reader written in PHP (or javascript) in it. I found where content is stored for Pages, but not the script who read this content. Pages uses the WYMEditor to render Pages:

Re: [galaxy-dev] Written tutorial

2011-12-29 Thread Jeremy Goecks
, Thank you for the link. I have already seen those files, but I was hoping for written instructions and the files for the video tutorials. Jeff On 12/29/2011 09:19 AM, Jeremy Goecks wrote: Jeff, Here are some tutorials on basic NGS analyses in Galaxy: http://main.g2.bx.psu.edu/u/james/p

Re: [galaxy-dev] Written tutorial

2011-12-29 Thread Jeremy Goecks
Jeff, Here are some tutorials on basic NGS analyses in Galaxy: http://main.g2.bx.psu.edu/u/james/p/exercises Best, J. On Dec 22, 2011, at 11:54 AM, Rosenfeld, Jeffrey wrote: Hi, I am trying to teach some people to use Galaxy, and I was looking for a written tutorial that went

Re: [galaxy-dev] defining genome assembler in galaxy

2011-12-29 Thread Jeremy Goecks
. Could you please guide me to the link/webpage that talks about checking the job manually using top/qstat/similar. Happy New Year, Bassam Tork. On Thu, Dec 29, 2011 at 9:18 AM, Jeremy Goecks jeremy.goe...@emory.edu wrote: My idea was to pass all above including main.bash as parameters

Re: [galaxy-dev] [galaxy-user] (OperationalError) unable to open database file

2012-01-05 Thread Jeremy Goecks
The manual highly recommend postgresql, is there any critical point to use it, instead of mysql? I am just more familiar with mysql. Thanks. The Galaxy team has found that, when running Galaxy, postgres is more stable and robust than mysql. Also, we use postgres ourselves, so it's

Re: [galaxy-dev] [galaxy-user] Make Galaxy continue running when i close the browser

2012-01-05 Thread Jeremy Goecks
Efthymois, You'll want to run Galaxy as a daemon process. Run % sh run.sh --help to get more information on running Galaxy as a daemon. Also, please direct questions about running/configuring a local Galaxy instance to galaxy-dev (cc'd) rather than galaxy-user, which is for tool and analysis

Re: [galaxy-dev] Fwd: tool restrict access

2012-01-05 Thread Jeremy Goecks
Ivan, #if $__user_email__ == displayYou are not authorized to use this tool/display #else command interpreter=python data_source.py $output $__app__.config.output_size_limit /command To make this approach work, the email check should go in the command tag. More

Re: [galaxy-dev] Fwd: tool restrict access

2012-01-09 Thread Jeremy Goecks
Ivan, tool name=UCSC Main id=ucsc_table_direct1 tool_type=data_source descriptiontable browser/description command interpreter=python #if $__user_email__ == displayYou are not authorized to use this tool/display #else data_source.py $output

Re: [galaxy-dev] User name / public name confusion.

2012-01-11 Thread Jeremy Goecks
Good catch Jeroen. It's fixed on the development branch in changeset 0d1d62b8be2e J. On Jan 11, 2012, at 9:04 AM, J. F. J. Laros wrote: Dear developers, Subject: Minor issue - Confusion about public / user name. Description: When creating an account or managing user information, the

Re: [galaxy-dev] Fwd: tool restrict access

2012-01-13 Thread Jeremy Goecks
May you suggest me how to sketch a simple python script that prints in the central section of the galaxy window a message like you are not authorized to execute this tool ? This isn't possible right now, hence my reference to the open Bitbucket issue regarding this limitation. The best you

Re: [galaxy-dev] An error occurred...

2012-01-16 Thread Jeremy Goecks
Milos, Can you share your history with me (Options - Share or Publish - share with a user -- my email address) and I'll take a look. Thanks, J. On Jan 16, 2012, at 12:46 PM, Milos Busarcevic wrote: Hi, I've got this message after trying to run Cufflinks on the Main instance: Server

Re: [galaxy-dev] An error occurred...

2012-01-17 Thread Jeremy Goecks
try to run cufflinks has changed into: Error executing tool: 'hg19'. Thanks, Milos On Mon, Jan 16, 2012 at 7:21 PM, Jeremy Goecks jeremy.goe...@emory.edu wrote: Milos, Can you share your history with me (Options - Share or Publish - share with a user -- my email address) and I'll

Re: [galaxy-dev] problems with location of filter bar in trackster

2012-01-18 Thread Jeremy Goecks
Hans, A couple questions: (a) What browser/OS are you using when seeing this problem? (b) Can you replicate this behavior on our public server? A couple notes: depending on your Web server setup, it may be necessary to use the packed Trackster script rather than the full one. Also, we're

Re: [galaxy-dev] problems with location of filter bar in trackster

2012-01-18 Thread Jeremy Goecks
it works fine on the public server Good, this narrows down the problem to installing the -central script on your instance b/c main's copy of the script is very similar to -central's. what do you mean by packed Trackster script All JavaScript files are compressed in

Re: [galaxy-dev] naming of history steps

2012-01-31 Thread Jeremy Goecks
Brad, But I think it might be a lot easier to manage if step names were based on the titles of the history items instead of data 2 or whatever. Has this been tried and rejected for some reason? It's been tried and rejected because dataset names get very long and unwieldy. E.g. Sam/Bam

Re: [galaxy-dev] naming of history steps

2012-02-02 Thread Jeremy Goecks
Ususally the most important information is the first and last step E.g. The TopHat run should be called TopHat on SOLiD 24A The alignment stats should be SAM/BAM Summary Metrics of Solid 24A With the rest of the tools in the chain identified in the more information box. This would

Re: [galaxy-dev] Feature requests - viewing data and annotations

2012-02-05 Thread Jeremy Goecks
Hi Shaun, Restricting datasets to show only the first megabyte is a great idea when dealing with large data files but sometimes I want to see the end of a file to check it is complete or to check the progress of a running job. At the moment I use the helper script to find the file on the

Re: [galaxy-dev] advanced parameters: all or nothing?

2012-02-10 Thread Jeremy Goecks
On Thu, Feb 9, 2012 at 11:27 PM, Andrew Warren anwar...@vbi.vt.edu wrote: Hello, I was recently adjusting advanced parameters when running Tophat in Galaxy and noticed that when advanced parameters are used, every field is converted and submitted as command line parameter to the tool at

Re: [galaxy-dev] advanced parameters: all or nothing?

2012-02-10 Thread Jeremy Goecks
Wouldn't it be better not to have the default value 40 (or 20) in the wrapper at all? i.e. Leave it out, so that by default that argument isn't used, so that tophat uses the default it wants to use. This would work but limits reproducibility and transparency because Galaxy wouldn't have a

Re: [galaxy-dev] generate dynamic select list based on other input dataset

2012-02-13 Thread Jeremy Goecks
Holger, Have you looked at how dynamic options work and whether they would be sufficient for your use? See thefilter tag syntax for details: http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Config%20Syntax#A.3Cfilter.3E_tag_set To specifically address your problem: can you determine the particular

Re: [galaxy-dev] Error executing tool: 'hg19'

2012-02-13 Thread Jeremy Goecks
Hello Joachim, After a quick try with visualising track in Trackster (importing one chromosome of hg19 - which did not succeed BTW), none of the tools in my local galaxy appear to work. What are the steps you're taking to produce this issue? They all send this error message: Error

Re: [galaxy-dev] Error executing tool: 'hg19'

2012-02-13 Thread Jeremy Goecks
This bug has been fixed in galaxy-central but, as per one of the threads you found, has not made it to galaxy-dist yet. Try this: 1. assuming you haven't made any changes to datatypes_conf.xml, copy datatypes_conf.xml.sample to datatypes_conf.xml 2. visit the custom builds page (User tab --

Re: [galaxy-dev] Error executing tool: 'hg19'

2012-02-14 Thread Jeremy Goecks
with that user. FYI, the reset of the datatypes_conf.xml did not do the trick: following error appears on the custom builds page: NoConverterException: Conversion from 'fasta' to 'len' not possible Thanks, Joachim On 02/13/2012 06:02 PM, Jeremy Goecks wrote: This bug has been fixed

Re: [galaxy-dev] advanced parameters: all or nothing?

2012-02-21 Thread Jeremy Goecks
So is the behavior of the optional=true tag that if the field form is left empty for that parameter then it will not be passed to the tool? Optional is enforced at the UI level; a wrapper can choose to handle optional parameters differently. I am wondering if a more sophisticated behavior

Re: [galaxy-dev] Trackster traceback on C. Cerevisiae mapped data

2012-03-01 Thread Jeremy Goecks
Alex, Generally cPickle errors when loading summary trees are a result of indexing gone bad. Here are a couple notes that should save you some work: *your bowtie indices and 2bit files do not need to be rebuilt; they are not generating these errors; *converting your SAM files to BAM may

Re: [galaxy-dev] Trackster cannot display INTERVAL or GATK-INTERVAL files???

2012-03-19 Thread Jeremy Goecks
Thon, It seems that trackster does not know how to display INTERVAL files? Is that true? Yes, this is currently a limitation. However, most of the work is done that is necessary to make this happen, so it should be done soon, definitely in the next month or two. Surely there is an easy way

Re: [galaxy-dev] refresh_on_change is broken?

2012-03-20 Thread Jeremy Goecks
Leandro, refresh_on_change is used quite a bit throughout the tool UI and appears to work fine both on a local instance and on the public instance. E.g. look at Tophat and change settings from 'Use Defaults' to 'Full Parameter List' -- the refresh_on_change works on reload the tool with more

Re: [galaxy-dev] refresh_on_change is broken?

2012-03-21 Thread Jeremy Goecks
Maybe we don't understand the feature in the documentation, but this isn't the refresh_on_change functionality. In the Tophat tool XML there are no refresh_on_change=true attributes, you are talking about a conditional tag which when changing will refresh the page in order to expose

Re: [galaxy-dev] potential bug in DefaultToolAction collecting chrom info

2012-03-22 Thread Jeremy Goecks
Andrew, You are correct. I've made this change in galaxy-central changeset 4d48285fcaa6 Thanks, J. On Mar 20, 2012, at 9:18 PM, Andrew Warren wrote: Hello, In the file lib/galaxy/tools/actions/__init__.py for the execute function of the DefaultToolAction class line 198 has the following

Re: [galaxy-dev] refresh_on_change is broken?

2012-03-23 Thread Jeremy Goecks
A final pointer: to see tools that use dynamic options, take a look at Extract GFF features and Filter GFF dataset by feature count Good luck, J. On Mar 22, 2012, at 12:15 PM, Greg Von Kuster wrote: It's always best to look at examples in the code base for technical details like this rather

Re: [galaxy-dev] Formatting has lost the horizontal rule dividers in new Galaxy style

2012-03-27 Thread Jeremy Goecks
Greg, 1. The horizontal rule dividers specified by a - in the text have disappeared. Fixed in galaxy-central changeset cb5ff5b1bd67 2. Also I spotted a change where this .. - line 1 of some bullets - line 2 of some bullets Following line. .. lost the blank line after the line

Re: [galaxy-dev] Cufflinks inconsistency ...

2012-03-27 Thread Jeremy Goecks
Kory, Cufflinks quantifies transcripts in parallel, and the inconsistencies that you're seeing are likely the result of these processes finishing in different order during different runs. To address this issue, you can use the Galaxy sort tool. Ultimately, this is not a Galaxy issue but an

Re: [galaxy-dev] Create and Transfer Galaxy Page

2012-04-17 Thread Jeremy Goecks
Hi Todd, [Not sure if this is better suited to galaxy-dev or -user, so I'm sending to both]. galaxy-user is most appropriate for this question because it related to usage of Galaxy; galaxy-dev is for local installation and tool development questions. My question is - can I create a

Re: [galaxy-dev] Potential database corruption with local galaxy instance

2012-04-17 Thread Jeremy Goecks
Simon, Error executing tool: (ProgrammingError) column params of relation job does not exist This is your problem. This column should have been created in migration script 93, but for some reason it wasn't. The first step is to determine if subsequent migration scripts failed as well. Run

Re: [galaxy-dev] How to specify RUM output dir?

2012-04-18 Thread Jeremy Goecks
I’m good with everything except the output dir. I can’t figure out how to pass that directory information from Galaxy to the RUM_runner.pl in such a way that Galaxy will be able to subsequently capture the data. Does anybody have any advice, or can someone point me towards an existing

Re: [galaxy-dev] Displaying genomic sequences in Trackster

2012-04-18 Thread Jeremy Goecks
Hi Naharajan, My best guess is that you've found some bugs. Fortunately, they are likely fixed in galaxy-central and will soon be available in galaxy-dist (we're planning an update in the next couple days). Once galaxy-dist is updated, please try updating your Galaxy instance and seeing if

Re: [galaxy-dev] Potential database corruption with local galaxy instance

2012-04-18 Thread Jeremy Goecks
% sh manage_db.sh downgrade 92 % sh manage_db.sh upgrade The downgrade to 92 and upgrade created job.params. This is progress. You should be able to run jobs again, yes? Unfortunately, we are still getting errors about duplicate key values. The debug output when I try to export a history

Re: [galaxy-dev] Default annotation track

2012-04-19 Thread Jeremy Goecks
Alex, It's not clear what the problem is. Trackster will handle an arbitrarily large number of contigs (albeit somewhat clumsily). What contig annotation data are you trying to preserve and/or provide access to? J. On Apr 19, 2012, at 11:30 AM, Oleksandr Moskalenko wrote: I needed to add a

Re: [galaxy-dev] How to specify RUM output dir?

2012-04-19 Thread Jeremy Goecks
yes: standard output: Broken pip yes: write error I know what this means, generally, but not in the context of Galaxy. Is this a telltale symptom, or is it too generic to say? Hard to say, but it's coming from your script and is indeed causing your job to fail. You might find it easier to

Re: [galaxy-dev] linking Galaxy and Integrated Genome Browser

2012-04-19 Thread Jeremy Goecks
Hiral, I've cc'd the Galaxy development mailing list, which includes folks with experience in all areas of Galaxy. Can you be clear about what you're trying to do and what approach you're taking? Once it's clear what the issue is, someone can chime in with suggestions. Best, J. On Apr 19,

Re: [galaxy-dev] putting up a SNP module on galaxy

2012-04-23 Thread Jeremy Goecks
Hi Xiangyu, This question is best directed to the galaxy development list, which I've cc'd. In general, to integrate a tool into Galaxy, you'll want to write a tool wrapper: http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Config%20Syntax and then upload it to the Galaxy toolshed:

Re: [galaxy-dev] ERROR on History export

2012-04-26 Thread Jeremy Goecks
Hi Todd, History export is a beta feature and hasn't been fully tested yet. We'll look into this, but it's difficult to diagnose a bug on a local instance. Can you reproduce on either our main or test server? Thanks, J. On Apr 25, 2012, at 8:08 PM, Todd Oakley wrote: Hello, I'd am

Re: [galaxy-dev] New Tool - Can provide a template file to user

2012-04-26 Thread Jeremy Goecks
Either user can upload a spreadsheet for that or I shall provide a template file to fill up the data. When I checked there is no provision for uploading a speadsheet in my galaxy instance. Two simple options: (1) have users convert their spreadsheet data to csv/tabular format; Galaxy

Re: [galaxy-dev] refresh_on_change is broken?

2012-05-07 Thread Jeremy Goecks
Let me give an example of something that I do. I have the user select a file after which I, using dynamic_options, extract the column names and present those to the user with a select input parameter. With the selected column name I then present a new select input parameter with all the

Re: [galaxy-dev] Error when exporting history to file

2012-05-07 Thread Jeremy Goecks
Sarah, What tool was used to create the problematic dataset? Thanks, J. On May 7, 2012, at 12:17 PM, Sarah Diehl wrote: Hi all, I get the following error when I try to export a specific history to file: 10.1.5.190 - - [07/May/2012:18:01:08 +0200] GET /history/export_archive HTTP/1.1

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