Re: [galaxy-user] Galaxy User List Being Retired on June 6, 2014.

2014-06-02 Thread Daniel Blankenberg
I think this is great. Do we have it clearly explained on the wiki somewhere about how to turn on mailing list mode within biostar? I didn’t see anything in a quick search, but it would probably help ease the transition for people that just love the mailing list approach. I also find it

Re: [galaxy-user] galaxy-user Digest, Vol 95, Issue 9

2014-05-24 Thread Les Dethlefsen
Actually, if the ones I ordered last year are expiring soon, I'd suggest people continue to use those before recently ordered kits. Les - Original Message - Send galaxy-user mailing list submissions to galaxy-user@lists.bx.psu.edu To subscribe or unsubscribe via the World

Re: [galaxy-user] Galaxy 101 tutorial

2014-04-21 Thread Jennifer Jackson
Hello Carrie, Yes. UCSC changed the way that they organized their track groups, breaking out Variation and Repeats into two distinct categories. When doing the Table browser query, simply pick Variation (instead of Variation and Repeats) from the group drop down menu. The dbSNP track will be

Re: [galaxy-user] Galaxy + user program unable to locate files

2014-02-27 Thread Peter Cock
On Thu, Feb 27, 2014 at 9:32 AM, do kadya doka...@gmail.com wrote: Hi, Thank You for your kind reply. I am having problem in running command in Galaxy. my current command: tool_name file_having_multiple_file_name_tab_delimited.txt or .tab file1.bam file2.bam /usr/bin/x_tool

Re: [galaxy-user] Galaxy + user program unable to locate files

2014-02-27 Thread do kadya
Respected Sir, I don't think so that problem is in tool, because when I run same tool of normal terminal, it generate output. What I am thinking is: Consider Galaxy is running from its own directory, my files are in */home/user/Documents/* While uploading file, I am choosing for Link to files

Re: [galaxy-user] Galaxy + user program unable to locate files

2014-02-27 Thread Peter Cock
On Thu, Feb 27, 2014 at 4:20 PM, do kadya doka...@gmail.com wrote: I don't think so that problem is in tool, because when I run same tool of normal terminal, it generate output. This is why I was asking about Perl environment variables - they may be different compared to when you run the tool.

Re: [galaxy-user] Galaxy job is waiting to run forever

2014-02-17 Thread Martin Čech
Hello Martina, how did you try to upload it and on what server? I assume it was usegalaxy.org but please confirm. DId you use the upload button on the main page or *Get Data / Upload File* from the tool menu? How big is the file? thanks Martin, Galaxy team On Mon, Feb 17, 2014 at 11:37 AM,

Re: [galaxy-user] Galaxy job is waiting to run forever

2014-02-17 Thread Martin Čech
Hi Martina, this is the actual URL of the file: ftp://ftp.ddbj.nig.ac.jp/ddbj_database/dra/fastq/SRA046/SRA046351/SRX099052/SRR349685.fastq.bz2 The URL that you were using is the link to the folder, not the file (you can tell e.g. by the suffix of the file - my link has it, yours does not).

Re: [galaxy-user] Galaxy job is waiting to run forever

2014-02-17 Thread Martin Čech
Martina: it seems that the server you are downloading from has troubles communicating with Galaxy. This is probably FTP issue that we cannot control. The workaround is to download it yourself to your computer from the site and then upload it to Galaxy directly. Let me know how it goes regards

Re: [galaxy-user] galaxy-user Digest, Vol 91, Issue 21

2014-02-13 Thread Gang Wang
Hi, I just notice that NGS FASTQ Trimmerhttps://usegalaxy.org/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Ffastq_trimmer%2Ffastq_trimmer%2F1.0.0by column can't detect the fastq file I loaded. Anyone knows why. thanks a lot. On Fri, Jan 24, 2014 at 11:00 AM,

Re: [galaxy-user] Galaxy Pages - importing/downloading embedded data.

2013-11-07 Thread Jennifer Jackson
Hi Mo, Datasets are a bit tricky for the download/import steps on Pages. Do this: 1 - click on the dataset name to expand it 2 - you will see a message This dataset is large and only the first megabyte is shown below. | Show all. 3 - Click on the Show all. On the resulting view, the import

Re: [galaxy-user] galaxy-user Digest, Vol 89, Issue 4

2013-11-05 Thread Cory Dunn
Dear Graham, Thanks for the info. However, my problem is that the Tool Version field is completely empty in my history items (eg. Tophat2, Cuffdiff). I suppose I can check the dependancies list you described, but it would be important to know precisely which version was run on any given query.

Re: [galaxy-user] galaxy-user Digest, Vol 89, Issue 4

2013-11-05 Thread Jeremy Goecks
Thanks for the info. However, my problem is that the Tool Version field is completely empty in my history items (eg. Tophat2, Cuffdiff). I suppose I can check the dependancies list you described, but it would be important to know precisely which version was run on any given query. If

Re: [galaxy-user] [galaxy-dev] Bam File

2013-10-29 Thread Jeremy Goecks
Hello, First, I've moved this question from the Galaxy development mailing list to the Galaxy user mailing list; in the future, please send questions about using Galaxy to the galaxy-user list. To answer your question, files larger than 2GB must be uploaded via FTP to Galaxy. This is

Re: [galaxy-user] Galaxy available disk space bug

2013-10-08 Thread Jennifer Jackson
Hi Itys, The public server has been undergoing upgrades, and this included some data transfers. This has been going on behind the scenes for several months - I am sorry that your account was impacted and that we did not hear about it/solve it when the issue first began! When usegalaxy.org

Re: [galaxy-user] galaxy cloud: location of imported and converted fastq's

2013-10-07 Thread Dannon Baker
Hi PT, What exactly do you mean by Galaxy on the Cloud appears to expect that the imported and converted fastq files are in the same S3 bucket where the fastq.gz files were? Once imported from S3, you're correct in that everything will be on the local EBS volume. -Dannon On Sun, Oct 6, 2013

Re: [galaxy-user] [galaxy-dev] Adding a tool to Galaxy Main Server

2013-10-07 Thread Jennifer Jackson
Hello, New tools go into the Tool Shed, links with instructions are here: http://usegalaxy.org/toolshed http://wiki.galaxyproject.org/Tool%20Shed We tweet new tool additions to let the community know about them, info and recent tweets are here:

Re: [galaxy-user] Galaxy Main - Files not Found, Server Error

2013-09-23 Thread Jennifer Jackson
Hello Nathan, The view history issue has been resolved. However, there were known failures over the weekend. These jobs should be re-run. The NGS cluster is down at this moment due to a datacenter problem, but is expected to be restored shortly. Queued jobs will process when back up as

Re: [galaxy-user] Galaxy main server down?

2013-09-10 Thread Jennifer Jackson
Hello Ben, The public Main Galaxy server experienced issues leading to unexpected downtime over the weekend. These have since been resolved. Please let us know if problems persist. Our apologies for the inconvenience, Jen Galaxy team On 9/9/13 6:44 AM, Ben Ben wrote: Co-ask : When will

Re: [galaxy-user] Galaxy main server down?

2013-09-10 Thread Jennifer Jackson
Hello Boaz, The public Main Galaxy server experienced issues leading to unexpected downtime over the weekend. These have since been resolved. Please let us know if problems persist. Our apologies for the inconvenience, Jen Galaxy team On 9/9/13 2:03 AM, Boaz Shaanan wrote: Hi, Any idea

Re: [galaxy-user] Galaxy main server down?

2013-09-09 Thread Ben Ben
Co-ask : When will the Galaxy Server work again? Thanks for Galaxy Team's work and support! On Mon, Sep 9, 2013 at 5:03 AM, Boaz Shaanan bshaa...@bgu.ac.il wrote: Hi, Any idea when the server is going to be up again? Boaz Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion

Re: [galaxy-user] Galaxy Tool Version Question

2013-08-28 Thread Bjoern Gruening
Hi Scott, please click on the 'i' info icon in one of your datasets (next to the download button) you will find many additional information's, including the wrapper version and the version of the underlying tool. Cheers, Bjoern To Whom it May Concern, I was trying to find the version

Re: [galaxy-user] [galaxy-dev] Connecting to new Galaxy Cloudman by FTP

2013-08-27 Thread Enis Afgan
FTP issues are fixed now so things will be functional out of the box without any of these workarounds. On Tue, Jul 16, 2013 at 4:04 PM, Daniel Blankenberg d...@bx.psu.edu wrote: Hey Mo, You can use ssh to connect to the Galaxy machine. If you used cloudlaunch to create your instance, it

Re: [galaxy-user] galaxy-user Digest, Vol 86, Issue 17

2013-08-21 Thread Jun Fan
Hi Jen, Maybe I have not explained my question cleared which caused the confusion: I did not mean the name of dataset. Actually I asked about the display of the tool in the graphic view from the default value (guess it is the name attribute in the tool element of the wrapper) to something

Re: [galaxy-user] galaxy-user Digest, Vol 86, Issue 17

2013-08-21 Thread Dannon Baker
Hi Jun, Thanks for the clarification. Workflow step annotations would best suit your needs, I think. When you're in the editor, click on any step and on the right side you'll see a field Annotation / Notes which will be displayed when the workflow is viewed. This can be used to clear up any

Re: [galaxy-user] Galaxy server down?

2013-08-05 Thread Politz, Samuel M.
From: galaxy-user-boun...@lists.bx.psu.edu [galaxy-user-boun...@lists.bx.psu.edu] on behalf of Anto Praveen Rajkumar Rajamani [a...@hum-gen.au.dk] Sent: Monday, August 05, 2013 7:53 AM To: Elizabeth Clare; galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] Galaxy

Re: [galaxy-user] Galaxy error

2013-07-25 Thread Toni Delorey
Hello, Thanks for your response. I can almost never get anything done on the public instance of Galaxy. It can take an entire day to even load a.bed file. When I was encountering the error I was on the Broad Instance. Marco, I tried following the instructions above to ensure that genome was

Re: [galaxy-user] Galaxy error

2013-07-25 Thread Jennifer Jackson
Toni, As a quick work-around until set up natively, you may be able to use the Extract DNA tool with a custom reference genome: http://wiki.galaxyproject.org/Support#Custom_reference_genome See the example in: Tools on the Main server, should work on any Galaxy set up as a production server

Re: [galaxy-user] Galaxy error

2013-07-23 Thread Björn Grüning
Hi Toni, to convert a bed file to a fasta file you need the genomic sequence. You can specify a genome-build for every bed file in your history. You probably don't have a genome build specified or its specified as sacCer2. sacCer2 is not configured I would guess, so no genome is available. Try to

Re: [galaxy-user] Galaxy error

2013-07-23 Thread Jennifer Jackson
Hi Toni, Bjoern is correct - if you are using a local Galaxy that does not have this reference genome available, it needs to be added. The Extract DNA tool requires a .2bit version of the genome available and the alignseq.loc file configured. More is here, section LASTZ and EXTRACT Genomic

Re: [galaxy-user] [galaxy-dev] Connecting to new Galaxy Cloudman by FTP

2013-07-16 Thread Daniel Blankenberg
Hey Mo, You can use ssh to connect to the Galaxy machine. If you used cloudlaunch to create your instance, it should display an example connection string that will work from e.g. a linux/mac shell, something like: 'ssh -i cloudman_keypair.pem ubuntu@IP', after your instance launched. Once

Re: [galaxy-user] galaxy online difficulty?

2013-07-15 Thread Jennifer Jackson
Hello Elizabeth, Are you still having problems today, when using the public Main Galaxy instance at https://main.g2.bx.psu.edu/ (usegalaxy.org)? The server was very busy last week and wait times were variable depending on the job type. Allowing jobs to queue and run is still the best

Re: [galaxy-user] [galaxy-dev] Connecting to new Galaxy Cloudman by FTP

2013-07-15 Thread Mohammad Heydarian
Hey Nate, Thanks for the response and instructions. I understand the last three steps of your protocol, but the first step is difficult for me to understand. I'm guessing that, 1. Set 'use_pbkdf2 = False' in universe_wsgi.ini anywhere in the [app:main] section, is telling me to change a setting

Re: [galaxy-user] galaxy-user Digest, Vol 84, Issue 27

2013-06-28 Thread Batool Akhtar-Zaidi
Please unsubscribe Sent from my iPhone On Jun 28, 2013, at 12:01 PM, galaxy-user-requ...@lists.bx.psu.edu galaxy-user-requ...@lists.bx.psu.edu wrote: Send galaxy-user mailing list submissions to galaxy-user@lists.bx.psu.edu To subscribe or unsubscribe via the World Wide Web, visit

Re: [galaxy-user] Galaxy Error

2013-06-07 Thread Jennifer Jackson
Hello Delong ZHOU, On 6/7/13 8:23 AM, Delong, Zhou wrote: Hello, I'm using the main galaxy server to analyse some data and then I got this messege: An error occurred while getting updates from the server. Please contact a Galaxy administrator if the problem persists. This message indicates

Re: [galaxy-user] [galaxy-dev] Information for Galaxy Event Horizon

2013-06-03 Thread Dave Clements
Hi Sarah, We'd be happy to put this on the Galaxy Event Horizon page on the wiki. Since the event is over, we would list it in the past events section. Is there a web page for the workshop or slides that we can link to? And, for future reference, wiki update requests can be sent directly to

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-25 Thread Hans-Rudolf Hotz
Hi David I have not used or tested it myself yet, but: have you looked into Bio-Linux ? The latest release (Bio-Linux 7) comes with Galaxy pre-installed, see: http://nebc.nerc.ac.uk/tools/bio-linux/bio-linux-7-info Regards, Hans-Rudolf On 04/25/2013 12:52 AM, David Joly wrote: Thanks

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-25 Thread David Joly
I was pretty much interested in Bio-Linux. However, the version of Galaxy that comes with it is already outdated and there is no easy way to update it. It is possible to delete it and re-install a newer version, this would be another possibility... Le 2013-04-25 à 05:50, Hans-Rudolf Hotz a

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-24 Thread Dave Clements
Hi David, Using Galaxy to teach undergraduates is a long term interest of mine. Which, unfortunately, does not mean I have yet put a lot of thought into it. However, lack of thought hasn't stopped me yet. First, this topic was discussed in a breakout at last year's GCC:

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-24 Thread David Joly
Thanks Dave! The focus is clearly more like your second description. The course is not intended to show them how to use command lines, or how to navigate in a UNIX environment and how to program. In fact, I think some would call what I have in mind more of a computational biology course than a

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-24 Thread Dave Clements
Hi David, Galaxy does sound like a great match for this course. It could also play a (smaller) part in the other course you are considering. Part of the course could include installing Galaxy and wrapping other tools to put into it. We are a Python based framework I do strongly recommend you

Re: [galaxy-user] Galaxy Main jobs not running?

2013-03-20 Thread Nicole Cloonan
Thanks Jen, that's really good to know. Cheers, Nicole. From: Jennifer Jackson [mailto:j...@bx.psu.edu] Sent: Thursday, 21 March 2013 12:19 AM To: Nicole Cloonan Cc: galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] Galaxy Main jobs not running? Hello Nicole, The public Main Galaxy

Re: [galaxy-user] [galaxy-dev] not enough memory space on my galaxy session

2013-03-08 Thread Jeremy Goecks
Hello, Apologies for the slow reply. I've moved this thread to the galaxy-user mailing list because it centers on using Galaxy rather than developing it. 2. deleted the first files, like the first fastq files, but I'm affraid to have an error messages Deleting your fastq files after you

Re: [galaxy-user] [galaxy-dev] History not updating for multiple instances of Galaxy

2013-02-21 Thread Peter Cock
On Thursday, February 21, 2013, wrote: Further, when I try “register” I add an email address and password, but the system still doesn’t log me in ** ** Maybe this is a database configuration issue? ** ** I didn’t think I’d need to state any specific entries in universe as I am

Re: [galaxy-user] Galaxy on one computer not showing latest runs in history

2013-02-13 Thread Dannon Baker
Dikla, After refreshing the browser, the most up-to-date view of the history should definitely be available. Are you positive you're looking at the same history in both places? I would verify that there's nothing in Saved Histories that looks like what you're expecting. -Dannon On Feb 13,

Re: [galaxy-user] galaxy-user submission

2013-02-13 Thread Jennifer Jackson
Hello Oya, This wiki has links to where you can add yourself to the list. http://wiki.galaxyproject.org/MailingLists#Subscribing_and_Unsubscribing Other general info about mailing lists: http://wiki.galaxyproject.org/Support#Mailing_Lists Best, Jen Galaxy team On 2/13/13 11:07 PM, Oya

Re: [galaxy-user] Galaxy Join takes too long?

2013-02-12 Thread Jennifer Jackson
Hello Milad, I am not sure if you are using the public Galaxy Main server (https://main.g2.bx.psu.edu) or your own local computer, or if the jobs are yellow and running or still grey and in the waiting-to-run stage. If using Galaxy Main, and if actually running (dataset is yellow), this

Re: [galaxy-user] Galaxy Join takes too long?

2013-02-12 Thread Milad Bastami
Shahid Beheshti's university of Medical Science  From: Jennifer Jackson j...@bx.psu.edu To: Milad Bastami mi.bast...@yahoo.com Cc: galaxy-user@lists.bx.psu.edu galaxy-user@lists.bx.psu.edu Sent: Tuesday, February 12, 2013 6:56 PM Subject: Re: [galaxy-user

Re: [galaxy-user] Galaxy Help

2013-02-03 Thread Jennifer Jackson
Hi John, The current link is: http://wiki.galaxyproject.org/FTPUpload We changed our wiki base and many of the older links do not redirect. I'll let our wiki admin Dave know about this and see what can be done. If this comes up again soon, the base always redirects to

Re: [galaxy-user] [galaxy-dev] Workflows

2013-01-18 Thread Geert Vandeweyer
Hi, I've put together some exempels for activating and using the api. You can find them here: http://geertvandeweyer.zymichost.com/index.php?page=readid=29 Best regards, Geert vandeweyer Op 16-jan.-2013 om 02:52 heeft neil.burd...@csiro.au het volgende geschreven: Hi Sorry if these

Re: [galaxy-user] [galaxy-dev] Workflows

2013-01-18 Thread Dannon Baker
Geert, this is great stuff! One small correction -- the API is enabled by default as of revision 7022:8376ad08ae41 (April 2012). -Dannon On Jan 16, 2013, at 1:17 AM, Geert Vandeweyer geertvandewe...@gmail.com wrote: Hi, I've put together some exempels for activating and using the api.

Re: [galaxy-user] [galaxy-dev] Workflows

2013-01-16 Thread James Taylor
Neil, .../api/workflows is not a path in the galaxy directory, but a URL that you should access over HTTP. For example, if you login to Galaxy Test, and then access https://test.g2.bx.psu.edu/api/workflows you will get a json dict of all of your workflows, including the workflow id that you are

Re: [galaxy-user] galaxy test dataset listing

2013-01-04 Thread James Taylor
You are probably seeing the mtDNA re-sequencing datasets associated with this paper: http://genomebiology.com/2011/12/6/R59 -- James Taylor, Assistant Professor, Biology/CS, Emory University On Fri, Jan 4, 2013 at 10:46 AM, Sun, Wenping [USA] sun_wenp...@bah.com wrote: Hello, It is good

Re: [galaxy-user] galaxy cloudman login

2013-01-02 Thread Dannon Baker
To be clear, your admin user still works on this instance but another one does not? If this is the case, can you log in as the admin user (and, if not, simply register a new user and make it an admin) and take a look at the user list in the admin panel? To get to the user list when in Galaxy,

Re: [galaxy-user] galaxy cluster nodes from the cloudlaunch site

2012-12-27 Thread dannonbaker
The root size for the worker nodes doesn't matter. The only thing relevant to Galaxy here are the available compute units and memory. The only storage and working space used is actually attached to the master node (the EBS volume you configure at initial launch) and shared over NFS with the

Re: [galaxy-user] galaxy cloudman login and aws instance

2012-12-20 Thread Dannon Baker
It looks like you've worked on both the public galaxy site we provide (main.g2.bx.psu.edu) and your own cloud instance. Data on the public instance won't be automatically migrated to the cloud instance and vice versa. All work done on the cloud site will remain there and persist after

Re: [galaxy-user] Galaxy Question

2012-12-17 Thread Jennifer Jackson
Hello Dominique, Would the tool 'NGS: QC and manipulation - Barcode Splitter' meet your needs? Please see the tool's help for usage. Another option is to first covert the file to tabular with 'FASTA manipulation -FASTA-to-Tabular', then use the 'Filter and Sort - Filter' tool. The match

Re: [galaxy-user] Galaxy Question

2012-12-17 Thread Jennifer Jackson
Hi Dominique, This means that there were no results. My fault - the Filter tool is the wrong choice. The Select tool, as you were starting with, is better for this case. The issues you had originally were most likely with format or the regular expression. So, be sure to do the following this

Re: [galaxy-user] [galaxy-dev] How to add to add hg16 index into the build-in index / reference gnome list?

2012-12-12 Thread Jennifer Jackson
Hello Sachit, Instructions for setting up local indexes are in our wiki here: http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup Bowtie2 is not specifically listed, but the instructions for Bowtie and Bowtie2 are nearly identical. The same indexes are used for Bowtie2 and Tophat2. When

Re: [galaxy-user] [galaxy-dev] Problem in Manage data Library

2012-12-06 Thread Anthonius deBoer
I have a similar problem in the latest galaxy-dist version from Dec 3rd.When I run BWA or BOWTIE2 (but it probably is not limited to those) the resulting SAM/BAM file is shown to be empty and none of the metadata has been updated.When I do a manual "Auto-detect" in the "Edit Attributes" section of

Re: [galaxy-user] Galaxy server configuration - what is [server:main]?

2012-11-16 Thread Dannon Baker
That's just the section demarcation stating that everything following (until the next such bracketed line) is a property of the 'server:main' section -- there should be no need to change that, though you can certainly change any of the properties within the section as needed. -Dannon On Nov

Re: [galaxy-user] [galaxy-dev] Account had be deleted ?

2012-11-09 Thread Nate Coraor
On Nov 7, 2012, at 5:47 AM, Diam Hsu wrote: Dear Sir or Madam, I used to use Galaxy to analyze NGS data, but these two days I could't login my Galaxy account, it also told me that my account has been marked deleted, and asked me to contact the Galaxy administrator to restore my

Re: [galaxy-user] galaxy MAIN data problem

2012-10-18 Thread Jennifer Jackson
Hello Kshama, We would like to help. I looked for an account under your email address kasw...@masonlive.gmu.edu on the public Main Galaxy instance at http://main.g2.bx.psu.edu (http://usegalaxy.org), and did not find one, so I will tell you how to check for the data yourself, then you can

Re: [galaxy-user] Galaxy not showing my reference genome in Drop down for BowTie

2012-10-10 Thread greg
Thanks Jen. But it looks like the datatype was already set to fasta. I tried setting it again and saving but it didn't seem to help. Is there anything else I can try? thanks, Greg On Tue, Oct 9, 2012 at 9:10 PM, Jennifer Jackson j...@bx.psu.edu wrote: Hi Greg, Nice pic, it helps! My guess

Re: [galaxy-user] Galaxy not showing my reference genome in Drop down for BowTie

2012-10-10 Thread Dannon Baker
Looking at your screenshot, you have Use a built-in index selected. If you change this to Use one from the history, do you see your fasta file listed? -Dannon On Oct 10, 2012, at 9:02 AM, greg margeem...@gmail.com wrote: Thanks Jen. But it looks like the datatype was already set to fasta.

Re: [galaxy-user] Galaxy not showing my reference genome in Drop down for BowTie

2012-10-10 Thread greg
Yes that was it! I knew I was missing something obvious. Thanks, Greg On Wed, Oct 10, 2012 at 9:06 AM, Dannon Baker dannonba...@me.com wrote: Looking at your screenshot, you have Use a built-in index selected. If you change this to Use one from the history, do you see your fasta file

Re: [galaxy-user] Galaxy not showing my reference genome in Drop down for BowTie

2012-10-09 Thread Jennifer Jackson
Hi Greg, Nice pic, it helps! My guess is that the datatype is not assigned as fasta. To do this, click on the pencil icon for the custom reference genome dataset (#1) to bring up the Edit Attributes forms in the center panel. Then click on the Datatype tab and change this to be fasta and

Re: [galaxy-user] Galaxy in cloud (not Amazon)

2012-10-04 Thread Enis Afgan
Hi Anatoliy, If you're going to keep that cluster alive continuously, just set it up as per the page whose link you provided and you should be ok. If you plan on having multiple users have their own cloud clusters with Galaxy, you'll probably want to do what I suggested in my previous email. Good

Re: [galaxy-user] Galaxy error: can't fine fasta file

2012-09-17 Thread Jennifer Jackson
Hello Kenneth, Are you using BLAST+ in a local install or cloud instance? The problem may be that the query dataset needs to have the datatype assigned as fasta. To do this, click on the pencil icon for the dataset to reach the Edit Attributes form. Then either scroll down to (or click on the

Re: [galaxy-user] Galaxy error: can't fine fasta file

2012-09-17 Thread Jennifer Jackson
Hi Kenneth, Yes, target databases require indexes and *.loc file set-up. Please see this wiki for details. For Genbank data such as NR, FTP the pre-built indexes and use those (generating them with formatdb is not necessary). http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup See the

Re: [galaxy-user] Galaxy error: can't fine fasta file

2012-09-17 Thread Jennifer Jackson
Hi Kenneth, It is likely that the path is wrong in the .loc file. It has to point to the actual files, not just the directory. Inside here /9720/genome_references/ncbi/nr-protein-db is where all the nr.* files are? In that case, the path should be

Re: [galaxy-user] Galaxy CloudMan - Nodes can't make their own qsub calls?

2012-09-15 Thread Enis Afgan
Added: worker nodes are now submit hosts as well: https://bitbucket.org/galaxy/cloudman/changeset/b4c66783b42fb559b35b689363b34826d00ced34 This will be generally available with the next release of CloudMan is made (however, there is no immediate plans for doing so as quite a bit of other details

Re: [galaxy-user] Galaxy CloudMan - Nodes can't make their own qsub calls?

2012-09-13 Thread James Taylor
You probably need to sudo to sgeadmin (cloudman guys correct me if you have this setup differently). I don't see any reason not to make worker nodes submit hosts by default in a future cloudman release. -- jt On Thu, Sep 13, 2012 at 4:17 PM, greg margeem...@gmail.com wrote: As a follow up I

Re: [galaxy-user] Galaxy-installed - Localhost Not opening

2012-08-17 Thread Scott McManus
This is slightly different from Masaki's question since you cannot access Galaxy from the same machine that it's running on. What are Galaxy's host and port settings in universe_wsgi.ini? -Scott - Original Message - Hi all I've installed Galaxy on my Mac. The sh run.sh, run but

Re: [galaxy-user] galaxy-user Digest, Vol 74, Issue 15

2012-08-16 Thread De Kumar, Bony
-- Message: 6 Date: Wed, 15 Aug 2012 11:28:37 -0700 From: Jennifer Jackson j...@bx.psu.edu To: Mahtab Mirmomeni m.mirmom...@student.unimelb.edu.au Cc: galaxy-u...@bx.psu.edu Subject: Re: [galaxy-user] Galaxy toolshed-vcftools Message-ID: 502bea55.3090...@bx.psu.edu Content-Type: text/plain; charset

Re: [galaxy-user] Galaxy toolshed-vcftools

2012-08-15 Thread Jennifer Jackson
Hello Mahtab, Some vcftools are in development status on our test server under VCF Tools at: http://test.g2.bx.psu.edu/ Adding these and others (including the ones you mention) to the Tool Shed is under consideration by the Galaxy team, but a firm timeline is not set at this time. If your

Re: [galaxy-user] galaxy wiki error

2012-07-29 Thread Jennifer Jackson
Hello, Nice catch! I made the corrections (this time) but next time we encourage you to jump right in and contribute to the wiki directly! Create an account and become an editor... http://wiki.g2.bx.psu.edu/VettedEditorGroup Feel free to double check big content edits/changes you are thinking

Re: [galaxy-user] Galaxy Help!!

2012-07-12 Thread Jennifer Jackson
Hello Robby, It sounds like you are using the Galaxy instance run by the Huttenhower Lab at Harvard? At this link? http://huttenhower.org/galaxy/ If so, then at the bottom of the home page is a link you can use to contact the lab members about issues with the instance. (They probably have

Re: [galaxy-user] Galaxy main not running jobs..

2012-06-18 Thread Nate Coraor
On Jun 16, 2012, at 7:38 PM, Jayaraman, Shyam wrote: Hi, My username is shyamsundar19...@gmail.com Galaxy main is not running jobs since today morning. They stay queued (gray) forever. I deleted and then re queued them twice. Still nothing.. Is the server down or is something wrong

Re: [galaxy-user] Galaxy Reference Genome

2012-06-14 Thread Loraine, Ann
...@gmail.commailto:kristen.r...@gmail.com Cc: galaxy-u...@bx.psu.edumailto:galaxy-u...@bx.psu.edu Subject: Re: [galaxy-user] Galaxy Reference Genome Hello Kristen, Our RNA-seq tutorial and FAQ can help out with the general workflow: https://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise https

Re: [galaxy-user] Galaxy in AWS AMI

2012-06-13 Thread Lilach Friedman
and I terminated a created from the beginning new instanced for the last several hours (at least 5 different instances). All don't work. Lilach 2012/6/14 Lilach Friedman lilac...@gmail.com Hi, Can somebody please help me? I'm trying for hours to connect to Galaxy on Amazon EC2. I did

Re: [galaxy-user] Galaxy Reference Genome

2012-06-13 Thread Jennifer Jackson
Hello Kristen, Our RNA-seq tutorial and FAQ can help out with the general workflow: https://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise https://main.g2.bx.psu.edu/u/jeremy/p/transcriptome-analysis-faq And an iGenomes reference annotation GTF dataset for mm9 is in the Shared

Re: [galaxy-user] Galaxy in AWS AMI

2012-06-13 Thread Enis Afgan
Hi Lilach, It seems you ran into a bug that was introduced in the most recent CloudMan update. It's been fixed now. If you still have your instance up, the way to get around the issue is to simply strip off '/cloud' part of the URL and you'll get to Galaxy. Any new instances will work fine.

Re: [galaxy-user] Galaxy Reference Genome

2012-06-13 Thread Jennifer Jackson
mailto:j...@bx.psu.edu Date: Wed, 13 Jun 2012 16:12:27 -0700 To: Kristen Roop kristen.r...@gmail.com mailto:kristen.r...@gmail.com Cc: galaxy-u...@bx.psu.edu mailto:galaxy-u...@bx.psu.edu Subject: Re: [galaxy-user] Galaxy Reference Genome Hello Kristen, Our RNA-seq tutorial and FAQ can help out

Re: [galaxy-user] [galaxy-dev] Create and Transfer Galaxy Page

2012-04-19 Thread Jeremy Goecks
Todd, There's not an ideal solution for your situation. My suggestion: (a) set up a cloud instance with your tools + a Page and use the share-an-instance feature so that others can access your data, tools, histories, and page in a single place ( http://wiki.g2.bx.psu.edu/Admin/Cloud ); (b) put

Re: [galaxy-user] [galaxy-dev] Create and Transfer Galaxy Page

2012-04-17 Thread Jeremy Goecks
Hi Todd, [Not sure if this is better suited to galaxy-dev or -user, so I'm sending to both]. galaxy-user is most appropriate for this question because it related to usage of Galaxy; galaxy-dev is for local installation and tool development questions. My question is - can I create a

Re: [galaxy-user] Galaxy logo: licence? (blog article project)

2012-04-11 Thread Jennifer Jackson
Hello L-A, Galaxy is open-source for all components, the license is here: http://bitbucket.org/galaxy/galaxy-central/src/3f12abbd2394/LICENSE.txt And in case you haven't seen this wiki, section 3 lists some preferred link URLs: http://wiki.g2.bx.psu.edu/Citing%20Galaxy We'll send more

Re: [galaxy-user] Galaxy logo: licence? (blog article project)

2012-04-11 Thread Louise-Amélie Schmitt
Hello Jen, That helped a lot, thank you! I didn't know the license also applied to the images. Best, L-A Le 11/04/2012 15:45, Jennifer Jackson a écrit : Hello L-A, Galaxy is open-source for all components, the license is here:

Re: [galaxy-user] Galaxy Server running error in fetching sequences

2012-04-04 Thread Jennifer Jackson
Hello Hong, There is likely a problem with the formatting of your input file. BED and GFF format are very different, and this may be the problem. You will want to input a strict BED6 file to this tool: http://wiki.g2.bx.psu.edu/Learn/Datatypes#Bed

Re: [galaxy-user] Galaxy Server running error in fetching sequences

2012-04-04 Thread Jennifer Jackson
for the reply. I'll try BED6 and see in Galaxy. I would also want to try ENSEMBL API, because one of our publication is based on ENSEMBL database. Best, Hong Xu From: Jennifer Jacksonj...@bx.psu.edu Reply-To: To: hongxuhon...@zju.edu.cn Subject: Re: [galaxy-user] Galaxy Server running error

Re: [galaxy-user] Galaxy Server running error in fetching sequences

2012-04-03 Thread Anton Nekrutenko
Hong: I'm forwarding this to our official user mailing list. Please, use it in the future for your inquiries. Thanks! anton Anton Nekrutenko http://nekrut.bx.psu.edu http://usegalaxy.org On Apr 3, 2012, at 4:37 PM, xu hong wrote: Hi Anton, I'm a biological student using Galaxy sever

Re: [galaxy-user] [galaxy-dev] Tophat error

2012-03-20 Thread Jennifer Jackson
Hi David, I don't know if you are still having this problem or not, but I did a web search and found this thread on seqanswers from 2/16 that seems like a good match to the problem you were having: http://seqanswers.com/forums/showthread.php?p=65085 These scientists resolved the problem by

Re: [galaxy-user] [galaxy-dev] Welcome.html page

2012-03-12 Thread Greg Edwards
Hi, I've found that I need to be really aggressive with forcing a purge refresh of the browser when changing things like static/welcome.html and static/images/whatever.png. It might be a function of the nginx light-weight HTML renderer within Galaxy caching things on top of the normal caching.

Re: [galaxy-user] Galaxy unable to set metadata for GFF files

2012-03-05 Thread Jennifer Jackson
Hi Peera, I downloaded the file, stripped off extra comment lines (extra two at top starting with #! and one at bottom ##). I loaded this to Galaxy as text, and when I attempted to set datatype as GFF3 ran into the metadata issues. This links at GMOD have a GFF3 format specification:

Re: [galaxy-user] Galaxy unable to set metadata for GFF files

2012-03-04 Thread Peter Cock
On Sun, Mar 4, 2012 at 6:34 PM, Hemarajata, Peera hemar...@bcm.edu wrote: Dear all, I’m been trying to get Galaxy to recognize this GFF from NCBI ( ftp://ftp.ncbi.nih.gov/genomes/Bacteria/Lactobacillus_reuteri_JCM_1112_uid58875/NC_010609.gff) but it failed to recognize the format after I

Re: [galaxy-user] [galaxy-bugs] GI errors in the megablast table of results ?

2012-03-01 Thread Peter Cock
Hello all, Did this issue get resolved? If Sandrine was right about there being an off by one error in GI number in the BLAST tabular output, it could be a bug in 'legacy' blastall command. I say 'legacy' BLAST because that's what Galay's NGS 'megablast' tool is using internally (as opposed to

Re: [galaxy-user] galaxy - table browser fasta and bed format

2012-02-22 Thread Jennifer Jackson
Hello Rita, The UCSC Table Browser has a limit on the amount of output that can be extracted in any single query. Without seeing your history, my initial suspicion is that both of the queries timed out, the first sooner than the last. Comparing the number of items between the original UCSC

Re: [galaxy-user] [galaxy-bugs] GI errors in the megablast table of results ?

2012-01-25 Thread Guru Ananda
Dear Sandrine, Thanks for pointing out this issue. The BLAST databases we have on Galaxy are from last year, while those on NCBI website are the latest (Jan 2012). As pointed out on NCBI website ( http://www.ncbi.nlm.nih.gov/Sitemap/sequenceIDs.html), it appears that each time any change is made

Re: [galaxy-user] Galaxy Bowtie alignment stats report

2012-01-18 Thread Jennifer Jackson
Hello Eric, There are a few choices: flagstat BAM Index Statistics SAM/BAM Alignment Summary Metrics Use Options - Show Tool Search and enter the tool name (in the left tool panel) to locate these and other tools quickly. Best, Jen Galaxy team On 12/8/11 4:02 PM, Eric Guo wrote: Hello,

Re: [galaxy-user] Galaxy Cufflinks update?

2012-01-12 Thread Jennifer Jackson
Hello Sheena, Our apologies, Cufflinks was updated to version 1.3.0 this week and we did a poor job of giving notice to our community. You can expect to see a tweet and a news item today and an updated wiki by the end of the week. Again, we are very sorry that you had to discover this on

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