methods

Messages by Date

2013/04/25 RE: Plasmid mix in the clone - the mechanism? Dunowska, Magda
2013/04/24 Re: Plasmid mix in the clone - the mechanism? Pow Joshi
2013/04/24 Re: Plasmid mix in the clone - the mechanism? Vladimir Gainullin
2013/04/21 Re: 5x sds sample buffer become brown color while preparing Dr Engelbert Buxbaum
2013/04/20 Re: 5x sds sample buffer become brown color while preparing Christian Praetorius
2013/04/20 Re: Any experiences with Geneamp 9700 cycler? Christian Praetorius
2013/04/19 Re: Any experiences with Geneamp 9700 cycler? Duncan Clark
2013/04/18 5x sds sample buffer become brown color while preparing Sudheer Sangeetham
2013/04/18 Re: Any experiences with Geneamp 9700 cycler? Duncan Clark
2013/04/17 Re: RNA isolation from Formaldehyde fixed samples Pow Joshi
2013/04/17 Re: RNA isolation from Formaldehyde fixed samples Shahrzad Jalali
2013/04/17 Re: RNA isolation from Formaldehyde fixed samples Pow Joshi
2013/04/17 Re: RNA isolation from Formaldehyde fixed samples Nick Theodorakis
2013/04/17 Re: RNA isolation from Formaldehyde fixed samples Dr Engelbert Buxbaum
2013/04/16 Re: RNA isolation from Formaldehyde fixed samples Pow Joshi
2013/04/16 How do I apply the genome scale reconstruction for E. coli in my experiments Yoginee Budhkar
2013/04/16 Re: Any experiences with Geneamp 9700 cycler? Michael Sullivan
2013/04/16 Fwd: RNA isolation from Formaldehyde fixed samples Pow Joshi
2013/04/16 Fwd: RNA isolation from Formaldehyde fixed samples Shahrzad Jalali
2013/04/16 Re: RNA isolation from Formaldehyde fixed samples Shahrzad Jalali
2013/04/15 RNA isolation from Formaldehyde fixed samples Pow Joshi
2013/04/15 Retroviral vector packaging compatibility Ed Siefker
2013/04/11 Re: protein estimation Dr Engelbert Buxbaum
2013/04/09 Re: protein estimation Vladimir Gainullin
2013/04/09 Re: protein estimation kaj . stenberg
2013/04/08 Re: protein estimation Nick Theodorakis
2013/04/08 protein estimation Sudheer Sangeetham
2013/04/04 Molisch test gives green layer? Gerchman
2013/03/15 RE: LiCl precipitation? Hiranya Roychowdhury
2013/03/15 Quantum Yield of Ethidium Bromide? Ayesha Ihsan
2013/03/14 RE: LiCl precipitation? Hiranya Roychowdhury
2013/03/12 RE: Sad CO2 Incubator... Deitiker, Philip R
2013/03/12 Re: Sad CO2 Incubator... Cathal Garvey
2013/03/12 Re: Sad CO2 Incubator... WS
2013/03/11 Did anyone here used plasmids or cDNA clones from Origene? Wenduo Ye
2013/03/11 Sad CO2 Incubator... Yvonne Couch
2013/03/11 RE: LiCl precipitation? Siefker, Ed B.
2013/03/11 Re: LiCl precipitation? Michael Sullivan
2013/03/10 RE: LiCl precipitation? Hiranya Roychowdhury
2013/03/10 Re: LiCl precipitation? Michael L. Sullivan
2013/03/10 Re: LiCl precipitation? Michael L. Sullivan
2013/03/09 RE: LiCl precipitation? Hiranya Roychowdhury
2013/03/08 Re: LiCl precipitation? Ed Siefker
2013/03/08 RE: LiCl precipitation? Hiranya Roychowdhury
2013/03/08 Running formaldehyde gels in 1x MOPS buffer? Ron
2013/03/07 DNA extraction and PCR query Sonal Patel
2013/03/07 Re: protein Irit Rappley
2013/03/07 protein akhatr hussain
2013/03/01 96 well inserts? Gerchman
2013/02/27 His tag CBP TAG Imran KHAN
2013/02/26 DNA Purification JEWELLE GLASGOW
2013/02/19 Re: Affinity chromatography on NHS activated agarose with small peptides WS
2013/02/19 Affinity chromatography on NHS activated agarose with small peptides WS
2013/02/18 Re: FFPE tissue rehydration - which xylene? Nick Theodorakis
2013/02/17 RE: Does Taq accespt ATP as substrate? Jayakumar, R
2013/02/17 RE: FFPE tissue rehydration - which xylene? Hiranya Roychowdhury
2013/02/17 FFPE tissue rehydration - which xylene? Gerchman
2013/02/15 Does Taq accespt ATP as substrate? WS
2013/02/11 phenol/CH3 extraction--no emulsion? Ed Siefker
2013/01/31 Re: protein extinction coefficient Toufic El Arnaout
2013/01/31 Re: protein extinction coefficient Toufic El Arnaout
2013/01/31 Re: protein extinction coefficient kaj . stenberg
2013/01/30 Re: protein extinction coefficient Irit Rappley
2013/01/30 protein extinction coefficient Sudheer Sangeetham
2013/01/28 Re: Incorporation efficiency of alpha-P32 dNTP in PCR Dr Engelbert Buxbaum
2013/01/28 qPCR & NGS 2013 - EARLY BIRD registration peroid & ABSTRACT CALL - deadline 31st Jan 2013 Editor www.Gene-Quantification.info
2013/01/25 Incorporation efficiency of alpha-P32 dNTP in PCR Vishal Chaudhari
2012/12/21 Re: Amplifying gene of interest from clinical isolate !!! Cathal Garvey
2012/12/21 Re: Gel Purifying tRNA Cathal Garvey
2012/12/17 Gel Purifying tRNA Rachel Schmidt
2012/12/14 Re: Homebrew Dark Reader amber screen? Ho Leung Ng
2012/12/14 Re: Calculating Protein Concentration Dr Engelbert Buxbaum
2012/12/13 Re: Homebrew Dark Reader amber screen? TS Nowlan
2012/12/13 Re: Homebrew Dark Reader amber screen? Cathal Garvey
2012/12/12 Re: Homebrew Dark Reader amber screen? Pow Joshi
2012/12/12 Re: Homebrew Dark Reader amber screen? WS
2012/12/12 Re: Homebrew Dark Reader amber screen? Pow Joshi
2012/12/12 Homebrew Dark Reader amber screen? WS
2012/12/05 Expression vector for GST-tagged TEV protease Phelan, Paul J.
2012/12/01 before thinking about the contamination dora
2012/11/30 Re: Kanamycin Stability, & Mysterious Orange Colonies shamrock
2012/11/30 Re: [DIYbio] Re: Kanamycin Stability, & Mysterious Orange Colonies Cathal Garvey
2012/11/30 Re: Kanamycin Stability, & Mysterious Orange Colonies shreyans chordia
2012/11/29 Re: Kanamycin Stability, & Mysterious Orange Colonies Xabier Vázquez Campos
2012/11/29 Where to obtain a copy of RAPDFst? ke
2012/11/29 Kanamycin Stability, & Mysterious Orange Colonies Cathal Garvey
2012/11/19 siRNA Transfection Reagent for THP-1 Babak Memari
2012/11/16 Re: Methods Digest, Vol 90, Issue 2 WS
2012/11/16 Re: Methods Digest, Vol 90, Issue 2 sudheer sangeetham
2012/11/16 Removing RNAse from resuable tubes Ed Siefker
2012/11/15 Re: Ni-NTA column turns yellow colour WS
2012/11/15 Ni-NTA column turns yellow colour sudheer sangeetham
2012/10/29 Re: Haunted Biologic DuoFlow system? Dr Engelbert Buxbaum
2012/10/20 qPCR NEWS - October 2012 - focus on RNA integrity Editor www.Gene-Quantification.info
2012/10/12 Re: Joining two PCR products together nazmiqueen
2012/10/11 Re: Recombinant protein with specific cleavage in fungi Cathal Garvey
2012/10/09 Re: Recombinant protein with specific cleavage in fungi (toufic el arnaout) Toufic Arnaout
2012/10/09 RE : RNA shipment (WS) Perreault, Jonathan
2012/10/07 Antibodies against short peptide sequences (5mer) WS
2012/10/07 Re: RNA shipment WS
2012/09/27 Haunted Biologic DuoFlow system? Phelan, Paul J.
2012/09/24 Re: chromatography columns Dr Engelbert Buxbaum
2012/09/21 RE: RNA shipment Jayakumar, R
2012/09/21 Re: RNA shipment Cathal Garvey
2012/09/21 qPCR NEWS September 2012 - focus on CNA (circulating nucleic acids) Editor www.Gene-Quantification.info
2012/09/19 RE: RNA shipment Jayakumar, R
2012/09/18 RE: RNA shipment Hiranya Roychowdhury
2012/09/17 chromatography columns WS
2012/09/14 RNA shipment Peter Ellis
2012/09/11 G418 selection Mukherjee, Sumit
2012/09/04 Rheumatoid arthritis (RA) biomarker and drug discovery assays and protocols kar . sanchayita
2012/08/25 Re: S35 Pulse Chase analysis of an endogenously expressed glycoprotein Dr Engelbert Buxbaum
2012/08/25 Re: Folic Acid solubility in bicarbonate solutions Dr Engelbert Buxbaum
2012/08/17 Folic Acid solubility in bicarbonate solutions Justin Washizu
2012/08/17 S35 Pulse Chase analysis of an endogenously expressed glycoprotein Vladimir Gainullin
2012/08/16 qPCR NEWS - August 2012 - focus on single-cell qPCR Editor www.Gene-Quantification.info
2012/08/13 new forum to buy and sell equipment Mary
2012/08/11 RE: (no subject) Irit Rappley
2012/08/11 (no subject) Chris McDermott-Roe
2012/08/07 RE: Overgrown (3day) LB plates ok to use? Jayakumar, R
2012/08/07 Overgrown (3day) LB plates ok to use? David Liu
2012/08/02 Southern Blot hybridization question Vladimir Gainullin
2012/08/02 Krebs buffer cloudy Gretha Van Oorschot
2012/08/01 Re: Hello...problem of detecting proteins with CBB staining Dr Engelbert Buxbaum
2012/07/31 Re: Methods Digest, Vol 86, Issue 8 sudheer sangeetham
2012/07/30 RE: Hello...problem of detecting proteins with CBB staining Daniel Prieto
2012/07/30 RE: Hello...problem of detecting proteins with CBB staining Jayakumar, R
2012/07/30 Hello...problem of detecting proteins with CBB staining sudheer sangeetham
2012/07/27 Re: pGEM-T contamination Cathal Garvey
2012/07/26 qPCR NEWS - July 2012 - focus on microRNA Editor www.Gene-Quantification.info
2012/07/26 pGEM-T contamination Miguel Azevedo
2012/07/24 regarding pngase f treatment divya teja
2012/07/24 regarding the PNGase F treatment divya teja
2012/07/18 hair root RNA isolation JAVIER RUIZ EDERRA
2012/07/05 PCR TAopt calculator Laurent Boulanger
2012/06/27 RE: pI shift be Phosphorylation? Mike Autry
2012/06/26 RE: pI shift be Phosphorylation? Hiranya Roychowdhury
2012/06/26 pI shift be Phosphorylation? Gerchman
2012/06/25 Best nuclease for removing DNA from bacterial lysates? Phelan, Paul J.
2012/06/23 Re: removal of DMSO from plant extract Joshua Silverstein
2012/06/23 qPCR NEWS June 2012 - first announcement qPCR & NGS 2013 Editor www.Gene-Quantification.info
2012/06/23 removal of DMSO from plant extract Dr Omar Hamarsheh
2012/06/23 Doubt regarding protein dialysis and r-protein purification???? sudheer sangeetham
2012/06/21 recommendation for Fermenter for Pichia Pastoris ab
2012/06/21 Removal of Ethidium Bromide from Skin shiwin k.k
2012/06/09 Re: DNA sequence alignment Peter Ellis
2012/06/07 Amino acid free media required Sonal Patel
2012/06/04 Re: GATEWAY CLONING WS
2012/06/03 GATEWAY CLONING Philip O'Brien
2012/05/24 Re: cloning oligos Zhonglin Chai
2012/05/24 Re: regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation WS
2012/05/24 cloning oligos Ed Siefker
2012/05/21 RE: regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation (divya teja) Nikola Wenta
2012/05/21 RE: regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation Jayakumar, R
2012/05/21 regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation divya teja
2012/05/19 Re: Protein concentration with PEG Dr Engelbert Buxbaum
2012/05/19 Re: Trypsin and chemotrypsin colorimetric assay Dr Engelbert Buxbaum
2012/05/19 Re: Ammonium sulfate precipitation from solutions with high sugar content Dr Engelbert Buxbaum
2012/05/18 RE: DNA sequence alignment Jayakumar, R
2012/05/18 DNA sequence alignment Elyaseen Daniel
2012/05/18 Re: DNA sequence alignment Don Gilbert
2012/05/16 RE: Regarding primary antibody binding in western blot Jayakumar, R
2012/05/16 Re: Regarding primary antibody binding in western blot Irit Rappley
2012/05/16 Regarding primary antibody binding in western blot divya teja
2012/05/15 BarSpec Chrom-A-Scope anyone? wally
2012/05/04 Re: Some funny (?) comics Michael Sullivan
2012/05/04 Re: Some funny (?) comics Pow Joshi
2012/05/03 Re: Some funny (?) comics Cathal Garvey
2012/05/03 Some funny (?) comics WS
2012/05/03 Re: Make Concentrated Solutions of hydrophobic amino acids WS
2012/05/02 RE: Inoue 1990 original paper for SEM transformation Jayakumar, R
2012/05/01 Make Concentrated Solutions of hydrophobic amino acids Sonal Patel
2012/05/01 Re: Methods Digest, Vol 84, Issue 1 Nikola Loncar
2012/05/01 Re: Ammonium sulfate precipitation from solutions with high sugar content Pow Joshi
2012/05/01 Re: Ammonium sulfate precipitation from solutions with high sugar content Pow Joshi
2012/05/01 Re: Ammonium sulfate precipitation from solutions with high sugar content WS
2012/05/01 RE: Ammonium sulfate precipitation from solutions with high sugar content Irit Rappley
2012/05/01 Re: Protein concentration with PEG AllisonH
2012/04/30 Membrane_protein_purification with_HIC Theresa H
2012/04/30 Re: Ammonium sulfate precipitation from solutions with high sugar content WS
2012/04/30 Re: Ammonium sulfate precipitation from solutions with high sugar content Michael Sullivan
2012/04/30 Re: Ammonium sulfate precipitation from solutions with high sugar content Pow Joshi
2012/04/30 Trypsin and chemotrypsin colorimetric assay Gerchman
2012/04/30 Ammonium sulfate precipitation from solutions with high sugar content WS
2012/04/30 Protein concentration with PEG WS
2012/04/29 qPCR Newsletter - April 2012 with focus on RNA integrity Editor www.Gene-Quantification.info
2012/04/20 Re: oligonucleotide-polypetide conjugation WS
2012/04/20 Re: oligonucleotide-polypetide conjugation alexandra balliou
2012/04/20 Re: oligonucleotide-polypetide conjugation alexandra balliou
2012/04/20 Re: oligonucleotide-polypetide conjugation WS
2012/04/19 oligonucleotide-polypetide conjugation alexandra balliou
2012/04/13 A minimal medium for S. aureus RN4220 Morooj
2012/04/12 Digestion problems with NaeI Edward Valera
2012/04/06 Error prone PCR with nucleotide analogs and high fidelity polymerase David Liu
2012/03/29 Strain requirements for routine cloning in phagemid M13 vector? (eg. pSEX81) David Liu
2012/03/27 Re: Methods Digest, Vol 82, Issue 6 sudheer sangeetham
2012/03/26 Re: trouble with membrane protein expression Ho Leung Ng
2012/03/26 trouble with membrane protein expression heraa rajandas
2012/03/09 Re: Methods Digest, Vol 82, Issue 4 Michael Sullivan
2012/03/09 Re: Methods Digest, Vol 82, Issue 4 sudheer sangeetham